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Article

A Cluster of Peronospora parasitica 13-like (NBS-LRR) Genes Is Associated with Powdery Mildew (Erysiphe polygoni) Resistance in Mungbean (Vigna radiata)

by
Pitsanupong Waengwan
1,
Kularb Laosatit
1,
Yun Lin
2,
Tarika Yimram
1,
Xingxing Yuan
2,
Xin Chen
2 and
Prakit Somta
1,*
1
Department of Agronomy, Faculty of Agriculture at Kamphaeng Saen, Kasetsart University, Kamphaeng Saen 73140, Thailand
2
Institute of Industrial Crops, Jiangsu Academy of Agricultural Sciences, Nanjing 210014, China
*
Author to whom correspondence should be addressed.
Plants 2024, 13(9), 1230; https://doi.org/10.3390/plants13091230
Submission received: 4 April 2024 / Revised: 24 April 2024 / Accepted: 25 April 2024 / Published: 29 April 2024
(This article belongs to the Special Issue Advances in Legume Crops Research)
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Abstract

:
Powdery mildew (PM) caused by Erysiphe polygoni is an important foliar disease in mungbean (Vigna radiata). A previous study showed that QTL qPMRUM5-2 is a major locus for PM resistance in mungbean accession RUM5 (highly resistant). Bioinformatics analysis revealed that flanking markers of the qPMRUM5-2 covered a region of 1.93 Mb. In this study, we conducted fine mapping for the qPMRUM5-2 using the F2 population of 1156 plants of the cross between Chai Nat 60 (CN60; highly susceptible) and RUM5. PM resistance evaluation was performed under field conditions using F2:3 lines grown in three different environments. QTL analyses consistently located the qPMRUM5-2 to a 0.09 cm interval on linkage group 6 between InDel markers VrLG6-InDel05 and VrLG6-InDel10, which corresponded to a 135.0 kb region on chromosome 8 containing nine predicted genes of which five were NBS-LRR-type genes Recognition of Peronospora parasitica 13-like protein (RPP13L). Whole-genome re-sequencing of RUM5 and CN60 showed polymorphisms in four RPP13L genes predictively cause substantial amino acid changes, rendering them important candidate genes for PM resistance. The InDel markers VrLG6-InDel05 and VrLG6-InDel10 flanking to the qPMRUM5-2 would be useful for marker-assisted breeding of PM resistance in the mungbean.

1. Introduction

Mungbean (Vigna radiata [L.] Wilczek) is an important legume crop grown in tropical and subtropical regions of Asia. It is widely grown in South and Southeast Asia, with a production area of more than 7.0 ha [1]. Mungbean seeds contain high proteins and carbohydrates of about 20–28% and 65–75%, respectively. The seeds are cooked and consumed or processed into several products, such as bean sprouts, starch, and protein isolates/concentrates. At present, mungbean has become a popular source of plant-based proteins. Major producers of mungbean include India, Myanmar, China, Indonesia, Pakistan, Bangladesh, Thailand, Cambodia, the Philippines, and Australia. The crop is now also popularly grown in several countries in Africa and America. Mungbean is fast growing, has an early maturity duration (60–75 days), and relative drought tolerance, and thus, it is generally grown as a sole crop following cereals such as rice, corn, and wheat or as a component in several cropping systems such as intercropping with sugar cane [1]. However, the average yield of mungbean is very low, being about 720 kg, although the potential yield of improved cultivars is about 2 tons/ha or higher.
Plant diseases are major factors causing low seed yield in the mungbean. Powdery mildew (PM), Cercospora leaf spot, and yellow mosaic virus are common foliar diseases of mungbean grown in Asia. PM disease caused by the fungus Erysiphe polygoni D.C. is an important foliar disease of mungbean [2]. It causes yield losses of up to 20–40% in susceptible cultivars [2]. This disease generally prevails in the cool–dry season of the tropical and subtropical region, which is a major season for the production of high-quality mungbean in some South and Southeast Asian countries [3]. Therefore, breeding of PM-resistant cultivars is one of the important goals in mungbean breeding programs. Mungbean germplasm identified for PM resistance and genetics of the resistance have been well documented. All the sources of PM resistance are from India. Among the resistant germplasm reported, the accession “RUM5” is the most interesting as it has been reported to be immune to PM disease in India [4,5], Taiwan [6], Thailand [7], and China (Xin Chen, personal observation).
A classical genetic study showed that the PM resistance in RUM5 is governed by two dominant loci: Pm-1 and Pm-2 [4]. Genotypes possessing both Pm-1 and Pm-2 are immune to the disease, while those possessing Pm-1 are highly resistant, and those with Pm-2 are moderately resistant [4]. Quantitative trait locus (QTL) analysis using F2 and backcross populations derived from crosses between RUM5 and PM-susceptible cultivar “Chai Nat 60” (CN60) revealed three QTLs, including qPMRUM5-1, qPMRUM5-2, and qPMRUM5-3, controlling the PM resistance in RUM5 [7]. qPMRUM5-2 and qPMRUM5-3 were major loci and were consistently identified in both F2 and backcross populations, while qPMRUM5-1 was a minor locus and was only identified in one population [7]. Recently, Yundaeng et al. [3] showed that the gene VrMLO12 (LOC106773784), a homolog to AtMLO1 in Arabidopsis thaliana, on mungbean chromosome 9 encoding a Mildew Locus O protein is the candidate gene for the resistance at qPMRUM5-3. The MLO protein is well known for its involvement in powdery mildew disease in plants. The qPMRUM5-2 was localized between simple sequence repeat (SSR) markers CEDG169 and CEDG121 on the mungbean linkage group (LG) 6 [7]. Comparative linkage analysis showed that qPMRUM5-2 possibly be the same as the QTL qPMR-1 conferring PM resistance in the mungbean breeding line “VC6468-11-1A” that was reported by Kasettranan et al. [7,8].
To date, molecular function(s) and mechanism(s) of qPMRUM5-2 are not yet known. In this study, we report the molecular characterization of the qPMRUM5-2 in RUM5, which will be useful for the genetic improvement in PM resistance in mungbean by molecular breeding. The objective of this study is to finely map the qPMRUM5-2 and identify candidate genes for PM resistance.

2. Results

2.1. PM Disease Reaction in the F2 Population

The reaction of PM resistance of the F2 population was evaluated using 1156 F2:3 lines grown in an augmented design in Chai Nat (CN), Nakhon Pathom (NP), and Petchabun (PB). The results of the PM resistance evaluation are summarized in Table 1. Disease scores of the F2:3 lines in these locations ranged between 1.00 and 8.81, 1.00 and 7.58, and 1.00 and 7.50 with means of 4.28, 3.70, and 3.56, respectively. Average disease scores of the F2:3 lines in the three locations (combined data) varied from 1.00 to 7.44 with a grand mean of 3.85. The parents, RUM5 and CN60, were shown to be highly different in PM resistance. The disease score in RUM5 was 1.00 at all three locations, while that in CN60 was 7.63, 5.77, and 5.72 in that order, with a mean across the locations of 6.37. The frequency distribution of disease scores of the F2:3 lines is shown in Figure 1.

2.2. Physical Location of the qPMRUM5-2 and Development of Novel DNA Markers for Fine Mapping

To determine the location of qPMRUM5-2 on the reference genome of mungbean cultivar Sulv1, a BLASTN search was conducted to identify the location of the SSR markers CEDG169 and CEDG121 on LG6 that were previously identified as flanking markers to the qPMRUM5-2. The BLASTN search demonstrated that these SSR markers were physically on chromosome 8 at the positions 1,532,506 bp and 3,460,036 bp, respectively. Thus, the two markers covered a region of 1.928 Mb. In total, 191 new markers, including 161 SSR and 38 InDel markers, were developed from this 1.928 Mb region (Table S1). Marker screening in the RUM5 and CN60 showed that 98 markers revealed polymorphisms, while 14 SSR markers failed to amplify the DNA of these two mungbean accessions (Table S1). Nonetheless, 14 polymorphic markers (VrLG6-SSR02, VrLG6-SSR10, VrLG6-SSR15, VrLG6-SSR29, VrLG6-SSR40, VrLG6-SSR47, VrLG6-SSR54, VrLG6-SSR67, VrLG6-SSR78, VrLG6-SSR96, VrLG6-InDel04, VrLG6-InDel05, and VrLG6-InDel10) were selected and used to genotype the F2 population.

2.3. Fine Mapping of qPMRUM5-2

A partial linkage map of LG6 for the F2 was constructed using 13 polymorphic markers developed in this study. The LG6 was 5.47 cM in length (Figure 2). QTL analysis for the PM resistance in different locations and combined data consistently located the qPMRUM5-2 in the same location, which was 1.6 cM on the map between the InDel markers VrLG6-InDel10 and VrLG6-InDel05 (Table 1 and Figure 2). The qPMRUM5-2 accounted for 29.61, 22.79, 13.22, and 32.40% of the disease score variation in CN, NP, PB, and the combined data, respectively. It expressed additive and dominant effects of 1.27 and 0.10 in CN, 1.11 and 0.00 in NP, 0.60 and 0.15 in PB, and 1.00 and 0.00 across the three locations, respectively.

2.4. Identification and Analysis of Candidate Genes for the qPMRUM5-2

We determined physical locations of the markers on the LG6 by BLASTN analysis. The results are illustrated in Figure 3. Orders of markers on LG6 perfectly agreed with their physical locations on the chromosome 8. Markers VrLG6-InDel10 and VrLG6-InDel05 flanking the qPMRUM5-2 were only 135.0 Kb apart and were at the positions 2.192 Mb and 2.327 Mb on chromosome 8 in that order. There were nine predicted genes between the markers VrLG6-InDel10 and VrLG6-InDel05, in which five of them, including EVM0008427, EVM0028537, EVM0032804, EVM0016936, and EVM0018688 encoded putative Recognition of Peronospora parasitica 13-like protein (RPP13L), a disease resistance protein. In addition, two other genes, EVM0000318 and EVM0031008, encoding RPP13L protein, located very next to the 135.0 Kb QTL region. We considered these seven RPP13L genes as the candidate genes for the PM resistance at the qPMRUM5-2. They were designated VrRPP13L3, VrRPP13L4, VrRPP13L5, VrRPP13L6, VrRPP13L7, VrRPP13L1, and VrRPP13L2, respectively.
To investigate the sequence variations in the candidate genes between the resistant and susceptible parents, their genomes were re-sequenced. In total, 23.5 and 35.2 Gb raw re-sequencing data were obtained for CN60 and RUM5, respectively. The Q20 values of sequences of both CN60 and RUM5 reached >93%, while the Q30 values of sequences of both CN60 and RUM5 reached >83%. After aligning the sequences to the Sulv1 mungbean reference genome, 20.26 Gb and 29.04 Gb clean data of CN60 and RUM5, respectively, were used for further analysis. The data equaled genome coverage of ~38.7× and ~47.9×, respectively.
After filtration, a total of 3,876,952 high-quality SNPs and 375,548 InDels were identified between CN60 and RUM5. However, there existed 863 homozygous SNPs and 50 homozygous InDels in the 135.0 Kb QTL region (Tables S2 and S3). Of these SNPs, 428 (49.59%) were in the exons of six genes. Among these exonic SNPs, 471 (97.43%) presented in the five VrRPP13L genes: many nonsynonymous SNPs occurred in all the five RPP13L1 genes, while two stop-gain SNPs existed in the VrRPP13L4 gene, and three stop-gain SNPs presented in the VrRPP13L5 and VrRPP13L6 genes (Table 2, Table S2 and S3). In the case of the InDels, 11 (22.0%) of them were in the exons of four genes (Table 3). All except one InDel presented in three RPP13L1 genes, including VrRPP13L3, VrRPP13L5, and VrRPP13L6. Five frameshift InDels and one stop-gain InDel occurred in the VrRPP13L3 gene, while one each frameshift InDel presented in the genes VrRPP13L5 and VrRPP13L6 (Table 3). All the stop-gain SNPs and InDels occurred in RUM5.
In addition to the 135.0 Kb QTL region, the alignment also revealed 151 exonic SNPs and 4 exonic InDels in the VrRPP13L2 gene (Table 2, Table 3, Table S2 and S3) located only 1.84 Kb away from the gene VrRPP13L3 (Figure 3). Of the 151 SNPs, 6 cause nonsynonymous SNPs, and 3 were splice-site SNPs. Among the four InDels, one was frameshift InDel, and one was stop-gain InDel.

3. Discussion

PM caused by E. polygoni in mungbean is among the plant biotic stresses that were subjected to gene mapping research at the beginning of the crop genomics era 30 years ago [9]. Nonetheless, due to the limited genomic tools and resources in this crop, little of the molecular basis of the PM resistance in mungbean is known at present. The QTLs qPMRUM5-2 and qPMRUM5-3 have been previously identified as major loci controlling the PM resistance in the mungbean accession RUM5 [7]. The molecular basis of the resistance conferred by the qPMRUM5-3 was investigated recently in which MLO12 was identified as the candidate gene for the resistance at this QTL [3].
Previously, the qPMRUM5-2 was localized between the SSR markers CEDG169 and CEDG121 [7]. Based on the genome sequence of the mungbean cultivar Sulv1 [10], these two markers were nearly 2.0 Mb apart, and there were more than 150 genes in this region. In this study, by exploiting the Sulv1 genome sequence to develop new SSR and InDel markers and using a large F2 population for fine mapping, we successfully narrowed down the qPMRUM5-2 to a genome region of 135.0 Kb on chromosome 8 of Sulv1 (Figure 3). Since QTL analysis using data of PM resistance obtained from three different environments consistently identified the qPMRUM5-2 at the same position (Table 1 and Figure 2), the position of the qPMRUM5-2 detected in this study is highly accurate. Based on the reference genome sequence of Sulv1, the qPMRUM5-2 was located in a genomic region containing a cluster of seven VrRPP13L genes (VrRPP13L1—VrRPP13L7). These genes spanned a region of 146.4 Kb (Figure 3), and they were considered the candidate genes for the powdery mildew resistance in RUM5.
VrRPP13L is a homolog to the RPP13 gene, which is a plant disease resistance gene (R gene) that encodes proteins with nucleotide-binding site (NBS) and leucine-rich repeat (LRR) domains (NBS–LRR proteins). In Arabidopsis thaliana L., RPP13 is a simple locus conferring a broad resistance to downy mildew disease caused by the fungus Hyaloperonospora parasitica (Gaum.) (synonym Peronospora parasitica (Pers. ex. Fr.)) by providing hypersensitive response [11]. RPP13L genes have been shown to be associated with resistance to several diseases in various crops. For example, resistance to powdery mildew caused by Bgt in wheat (Aegilops biuncialis Vis.) cultivar [12] and by Blumeria graminis f.sp. tritici (Bgt) in barley (Hordeum vulgare L.) [13,14], root-knot disease caused by Meloidogyne incognita in common bean (Phaseolus vulgaris L.), and clover rot disease caused by Sclerotinia trifoliorum Bain & Essary in red clover (Trifolium pratense L.) [15]. Interestingly, a RPP13L gene, Phavu_004G036200g, has been identified as a candidate gene for a locus conferring resistance to powdery mildew caused by E. polygoni in common bean (Phaseolus vulgaris L.), a legume species genetically close to mungbean [16]. We conducted BLASTP analysis and found that Phavu_004G036200g is an ortholog to the VrRPP13L genes identified in our study.
Since the seven VrRPP13L genes are clustered in a small region of only 146.4 kb, and six of the genes (VrRPP13L2—VrRPP13L7) showed exonic sequence polymorphisms that may alter the function of RPP13L proteins between the mapping parents (Supplementary Table S2), it will be difficult to determine which one of them, all of them, or any combinations of them provide powdery mildew resistance in RUM5. Additional study using a large population size to further identify the causal gene(s) for the resistance at this gene cluster is necessary. Nonetheless, the diversity and evolution of the R genes and their loci (R genes cluster) in plants can stem from recombination, duplications/deletions, and positive selection [17,18]. Due to the fact that the sequences of individual VrRPP13L genes in the R-genes cluster identified in this study are highly homologous, those VrRPP13L may have evolved via duplications. In addition, clustering of those VrRPP13L genes can lead to the evolution of novel resistance specificities to E. polygoni by unequal crossing over and/or gene conversion. It is noteworthy that as compared to the Sulv1 reference genome, 88.4% of the sequence polymorphisms found in the seven VrRPP13L genes between RUM5 and CN60 were contributed by RUM5 (Supplementary Table S2). The high diversity of VrRPP13L genes in RUM5 may provide the high and broad resistance of this mungbean germplasm against the fungus E. polygoni.
RUM5 is a valuable genetic resource for breeding of PM resistance in mungbean because it showed high and stable resistance in India, Thailand, Taiwan, and China. qPMRUM5-2 and qPMRUM5-3 are the QTLs for the PM resistance in RUM5 [7]. In this study, we demonstrated that a cluster of VrRPP13L genes, R-gene loci, is associated with the resistance at the qPMRUM5-2. Yundaeng et al. (2020) [3] showed that VrMLO12 encoding a protein belonging to MLO clade II is responsible for the resistance at the qPMRUM5-3. MLO is known as a susceptibility gene (S-gene), whose loss of function leads to recessively inherited resistance [19]. Hence, it appears that the high and broad resistance in RUM5 is a combined effect of polymorphisms in both S-gene and R-gene. The VrMLO12 and the VrRPP13L genes cluster in RUM5 would be useful for genetic improvement of PM resistance in mungbean. An InDel marker, VrMLO12-Indel3, specific to the VrMLO12 gene from RUM5, was developed [3]. The SSR marker VrLG6-SSR29, together with InDel markers VrLG6-InDel-05 and VrLG6-InDel-10, flanked the VrRPP13L gene cluster (Figure 3). These four markers can be utilized for breeding PM resistance in mungbean by pyramiding the VrRPP13L genes cluster and VrMLO12 gene from RUM5 into susceptible mungbean cultivar(s) via marker-assisted selection.

4. Materials and Methods

4.1. Plant Materials and DNA Extraction

An F2 population comprising 1156 plants was developed from a cross between RUM5 (male parent) and CN60 (female parent). CN60 is a cultivar from Thailand and is highly susceptible to PM, whereas RUM5 is immune or highly resistant to PM [3,7]. In total, 1156 F2 plants and their parents were grown under a field condition and self-pollinated to produce F2:3 seeds. The total genomic DNA of the F2 plants and the parental plants was extracted from young leaves following a CTAB method described by Lodhi et al. [20]. The DNA was assessed for quality and quantity using a NanoDrop™ 2000 spectrophotometer (Thermo Fisher Scientific, Waltham, MA, USA).

4.2. Evaluation for Powdery Mildew Disease

The F2:3 lines and the parents were evaluated for PM resistance under field conditions in three locations of Thailand, viz. (1) Chai Nat Field Crops Research Center, Sappaya, Chai Nat province, (2) Kasetsart University, Kamphaeng Saen Campus, Kamphaeng Saen, Nakhon Pathom province, and (3) Ta Daeng, Nong Phai, Petchabun province. The evaluation at Chai Nat was conducted from late December 2020 to early March 2021, while the evaluation at Nakhon Pathom and Petchabun was carried out from late December 2021 to early March 2022. At all locations, the evaluation was performed using augmented design [21]. RUM5 and CN60 were used as “check” cultivars. CN60 was planted around the field as a natural spreader of the PM disease. The F2:3 lines and the parental lines were grown in a single row of 1.5 m with two plants per hill (24 plants/row). The intra-row and inter-row spacings were 12.5 cm and 50 cm, respectively.
At 20, 25, and 30 days after planting (DAP), plants were inoculated with spore suspensions prepared from PM-infected leaves. At 60 DAP, 10 plants of each entry were randomly selected and visually scored for PM disease reaction using a scale of 1 to 9, where 1 had no disease infection; 3 had 1–25% leaf area infected; 5 had 26–50% leaf area infected, 7 had 51–75% leaf area infected, and 9 had 76–100% leaf area infected [7,9]. The disease scoring was carried out by three members of staff.

4.3. Determination of the Physical Position of qPMRUM5-2 and Development of Novel DNA Markers for Fine Mapping

Since the qPMRUM5-2 was previously mapped to a region between SSR markers CEDG169 and CEDG121 [7], we identified chromosomal locations of the two markers by conducting a BLASTN search of their primer sequences against the genome sequence of mungbean cultivar Sulv1 [10] in order to determine the location of qPMRUM5-2 on the reference genome. The genome sequence between markers CEDG169 and CEDG121 was detected for SSRs using SSRIT [22], and then, primers were designed for the SSRs utilizing Primer3 [23]. The primers were screened for polymorphism between RUM5 and CN60. Polymerase chain reaction (PCR) was conducted in a total volume of 10 μL containing 5 ng of genomic DNA, 0.2 mM dNTPs, 1 U Taq DNA polymerase 2 mM MgCl2, and 1 × Taq buffer (Thermo Fisher Scientific Inc., Waltham, MA, USA), and 2.5 μM each of forward and reverse primers. Amplification was performed using SimpliAmp™ Thermal Cycler (Thermo Fisher Scientific, Waltham, MA, USA) programmed as follows: 94 °C for 2 min followed by 35 cycles of 94 °C for 30 s, 55 °C for 30 s, 72 °C for 30 s, and 72 °C for 10 min. PCR products were run on 5% polyacrylamide gel electrophoresis. DNA bands were visualized by silver staining. In total, 24 markers showing clear polymorphism were used to genotype all the F2 plants.
In addition, InDel markers were developed based for the InDel polymorphisms detected between CN60 and RUM by whole-genome resequencing and alignment (see Section 4.5). Primers design and polymorphisms screening for the InDel markers were performed using the same procedures described above for the SSR markers.

4.4. Linkage and QTL Analyses

The linkage map for the F2 population was constructed using the software QTL IciMapping 4.2 [24]. Markers were grouped with logarithm of odds (LOD) values of 5.0 and then ordered using REcombination Counting and ORDering (RECORD) method [25]. Distances between markers were calculated using Kosambi’s mapping function [26].
QTL for PM resistance was localized on the linkage map using the inclusive composite interval mapping method (ICIM) [27] by QTL IciMapping 4.2. A significant LOD threshold for declaring QTL was calculated by a 1000 permutation test at the probability of 0.001.

4.5. Candidate Genes Analysis

Markers flanking the qPMRUM5-2 were used to BLAST against the reference genome of mungbean cultivar Sulv1 [10] to determine of the QTL region. Genes located within and near the QTL region were considered candidate genes for the PM resistance in RUM5. In order to discover the DNA polymorphism(s) in the candidate genes, genomes of RUM5 and CN60 were re-sequenced as described by Arai-Kichise et al. [28]. In brief, Illumina pair-end sequencing (PE150) was performed. At least 1μg DNA of RUM5 and CN60 was used for the construction of paired-end sequencing libraries with insert sizes of ~450 bp. Purified DNA is sheared into smaller fragments with a desired size using Adaptive Focused Acoustics® technology (Covaris, Woburn, MA, USA) and T4 DNA polymerase was applied to generate blunt ends. After adding an ‘A’ base to the 3′ end of the blunt phosphorylated DNA fragments, adapters are ligated to the ends. The desired fragments were purified via gel electrophoresis and then selectively enriched and amplified by PCR. Index tags were introduced into the adapter at the PCR stage, followed by a library quality test. The amplified products were quantified by TBS-380 Fluorometer (Promega, Madison, WI, USA). Subsequently, the paired-end libraries were sequenced using Illumina NovaSeq PE150 (Illumina, San Diego, CA, USA) by Shanghai Biozeron Biotechnology Co., Ltd. (Shanghai, China). After sequencing, the raw paired-end reads were trimmed and quality controlled by Trimmomatic (http://www.usadellab.org/cms/?page=trimmomatic, accessed on 28 February 2023) with default parameters.
High-quality sequencing reads were aligned against the reference genome of Sulv1 [10] using BWA (http://bio-bwa.sourceforge.net, accessed on 20 February 2023) software. Sequence Alignment/Map (SAM) format files [29] were imported into SAMtools [29] (http://samtools.sourceforge.net, accessed on 28 February 2023) for sorting and merging and into Picard (http://broadinstitute.github.io/picard/, version 1.92, accessed on 28 February 2023) to remove duplicated reads. BAM file was used to identify SNPs and short InDel (1–10 bp) using Genome Analysis Toolkit (GATK) version 4.1.2.0 (McKenna et al., 2010) [30] with “UnifiedGenotyper” function (https://gatk.broadinstitute.org, accessed on 28 February 2023).

Supplementary Materials

The following supporting information can be downloaded at https://www.mdpi.com/article/10.3390/plants13091230/s1. Table S1: Details of SSR and Indel markers used in this study; Table S2: Single-nucleotide polymorphisms between Chai Nat 60 (CN60) and RUM5 in RPP13L1 genes located in and near the qPMRUM5-2 region; Table S3: InDel polymorphisms between Chai Nat 60 (CN60) and RUM5 in RPP13L1 genes located in and near the qPMRUM5-2 region.

Author Contributions

Conceptualization, P.S., K.L. and X.C.; methodology, P.W. and P.S.; validation, P.W.; formal analysis, P.W. and Y.L.; investigation, P.W., T.Y., K.L., Y.L. and X.Y.; resources, P.S. and C.X; data curation, P.S. and Y.L; writing—original draft preparation, P.S.; writing—review and editing, P.S.; visualization, P.W.; supervision, P.S.; project administration, T.Y., K.L. and X.Y.; funding acquisition, P.S. and X.C. All authors have read and agreed to the published version of the manuscript.

Funding

This research was funded by the NSRF via the Program Management Unit for Human Resources and Institutional Development, Research and Innovation (grant number B16F640185), and the China Agriculture Research System of MOF and MARA-Food Legumes (CARS-08).

Data Availability Statement

The whole-genome resequencing data generated in this study were deposited to the National Center for Biotechnology Information (https://www.ncbi.nlm.nih.gov) (Project accession number “PRJNA977077”). Other datasets generated during and/or analyzed during the current study are available from the corresponding author upon reasonable request.

Acknowledgments

This research was supported in part by the Graduate Program Scholarship from The Graduate School, Kasetsart University.

Conflicts of Interest

The authors declare no conflicts of interest. The funders had no role in the design of the study; in the collection, analyses, or interpretation of data; in the writing of the manuscript; or in the decision to publish the results.

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Plants 13 01230 g001
Figure 1. Frequency distribution of disease scores for resistance to powdery mildew disease caused by E. polygoni in 1156 F2:3 lines derived from the cross CN60 (susceptible) × RUM5 (resistant). The F2:3 lines and the parents were evaluated for resistance in three locations, viz. Nakhon Pathom (a), Chai Nat (b), and Petchabun (c). Average data of the disease scores from the three locations are shown in (d).
Figure 1. Frequency distribution of disease scores for resistance to powdery mildew disease caused by E. polygoni in 1156 F2:3 lines derived from the cross CN60 (susceptible) × RUM5 (resistant). The F2:3 lines and the parents were evaluated for resistance in three locations, viz. Nakhon Pathom (a), Chai Nat (b), and Petchabun (c). Average data of the disease scores from the three locations are shown in (d).
Plants 13 01230 g001
Plants 13 01230 g002
Figure 2. Logarithm of odds (LOD) graphs for the QTL qPMRUM5-2 controlling powdery mildew disease resistance detected by inclusive composite interval mapping in the F2:3 population of the cross CN60 (susceptible) × RUM5 (resistant) grown in three locations. Markers flanking the qPMRUM5-2 are highlighted in red.
Figure 2. Logarithm of odds (LOD) graphs for the QTL qPMRUM5-2 controlling powdery mildew disease resistance detected by inclusive composite interval mapping in the F2:3 population of the cross CN60 (susceptible) × RUM5 (resistant) grown in three locations. Markers flanking the qPMRUM5-2 are highlighted in red.
Plants 13 01230 g002
Plants 13 01230 g003
Figure 3. A comparative genome map illustrating the location of qPMRUM5-2 detected by this study (left) and the candidate genes for the qPMRUM5-2 on chromosome 8 of the mungbean reference genome (Sulv1) (right). Markers flanking the qPMRUM5-2 are highlighted in red. The candidate genes are bolded and highlighted in blue or green.
Figure 3. A comparative genome map illustrating the location of qPMRUM5-2 detected by this study (left) and the candidate genes for the qPMRUM5-2 on chromosome 8 of the mungbean reference genome (Sulv1) (right). Markers flanking the qPMRUM5-2 are highlighted in red. The candidate genes are bolded and highlighted in blue or green.
Plants 13 01230 g003
Table 1. Details of the qPMRUM5-2 on linkage group 6 conferring to powdery mildew disease caused by Erysiphe polygoni. The qPMRUM5-2 was detected using an F2 population of 1152 individuals derived from the cross-Chai Nat 60 × RUM5.
Table 1. Details of the qPMRUM5-2 on linkage group 6 conferring to powdery mildew disease caused by Erysiphe polygoni. The qPMRUM5-2 was detected using an F2 population of 1152 individuals derived from the cross-Chai Nat 60 × RUM5.
LocationYearPosition
(cM)		Flanking MarkerLOD
Score		PVE aAdd bDom c
Chai Nat20191.6VrLG6-InDel10—VrLG6-InDel0587.7129.611.270.10
Phetchabun20201.6VrLG6-InDel10—VrLG6-InDel0565.5222.791.110.00
Nakhon Pathom20201.6VrLG6-InDel10—VrLG6-InDel0534.2013.220.600.15
Combined 1.6VrLG6-InDel10—VrLG6-InDel0598.5332.401.000.00
a PVE = percentage of variance explained by the QTL; b Add = additive gene effect; c Dom = dominant gene effect.
Table 2. Summary of single-nucleotide polymorphisms identified between Chai Nat 60 (CN60) and RUM5 in RPP13L genes located in and near the qPMRUM5-2 region.
Table 2. Summary of single-nucleotide polymorphisms identified between Chai Nat 60 (CN60) and RUM5 in RPP13L genes located in and near the qPMRUM5-2 region.
GeneRegionNumber of SNPsTypes of Mutation
EVM0008427upstream5-
(VrRPP13L3)exonic229nonsynonymous SNP (158) and synonymous SNP (71)
3′UTR9-
intergenic71-
EVM0028537upstream4-
(VrRPP13L4)exonic51nonsynonymous SNP (31), synonymous SNP (18) and stop-gain SNP (2)
intronic39
EVM0032804upstream1-
(VrRPP13L5)5′UTR3-
exonic77nonsynonymous SNP (52), synonymous SNP (22), and stop-gain SNP (3)
intergenic53-
EVM0016936upstream20-
(VrRPP13L6)exonic35nonsynonymous SNP (21), synonymous SNP (11), and stop-gain SNP (3)
downstream21-
intergenic22-
EVM0018688upstream49-
(VrRPP13L7)5′UTR1-
exonic25nonsynonymous SNP (16) and synonymous SNP (9)
3′UTR3-
downstream21-
intergenic75-
EVM0000318
(VrRPP13L1)		intergenic9-
EVM0031008
(VrRPP13L2)		exonic151nonsynonymous SNP (102), synonymous SNP (46), and splicing-relevant SNP (3)
intronic75-
downstream23-
Table 3. Summary of insertions/deletions (InDels) identified between Chai Nat 60 (CN60) and RUM5 in RPP13L genes located in and near the qPMRUM5-2 region.
Table 3. Summary of insertions/deletions (InDels) identified between Chai Nat 60 (CN60) and RUM5 in RPP13L genes located in and near the qPMRUM5-2 region.
GeneRegionNumber of InDelsTypes of Mutation
EVM0008427exonic8nonframeshift InDel (2), frameshift InDel (5) and stop-gain InDel (1)
(VrRPP13L3)intronic1-
3′UTR1-
EVM0032804exonic1frameshift InDel
(VrRPP13L5)intergenic5-
EVM0016936upstream2-
(VrRPP13L6)exonic1frameshift InDel
downstream2-
intergenic4-
EVM0018688upstream10-
(VrRPP13L7)downstream5-
intergenic8-
EVM0031008exonic4nonframeshift InDel (2), frameshift InDel (1) and stop-gain InDel (1)
(VrRPP13L2)intronic7-
downstream2-
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Waengwan, P.; Laosatit, K.; Lin, Y.; Yimram, T.; Yuan, X.; Chen, X.; Somta, P. A Cluster of Peronospora parasitica 13-like (NBS-LRR) Genes Is Associated with Powdery Mildew (Erysiphe polygoni) Resistance in Mungbean (Vigna radiata). Plants 2024, 13, 1230. https://doi.org/10.3390/plants13091230

AMA Style

Waengwan P, Laosatit K, Lin Y, Yimram T, Yuan X, Chen X, Somta P. A Cluster of Peronospora parasitica 13-like (NBS-LRR) Genes Is Associated with Powdery Mildew (Erysiphe polygoni) Resistance in Mungbean (Vigna radiata). Plants. 2024; 13(9):1230. https://doi.org/10.3390/plants13091230

Chicago/Turabian Style

Waengwan, Pitsanupong, Kularb Laosatit, Yun Lin, Tarika Yimram, Xingxing Yuan, Xin Chen, and Prakit Somta. 2024. "A Cluster of Peronospora parasitica 13-like (NBS-LRR) Genes Is Associated with Powdery Mildew (Erysiphe polygoni) Resistance in Mungbean (Vigna radiata)" Plants 13, no. 9: 1230. https://doi.org/10.3390/plants13091230

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