Comprehensive Proteome Profiling of a Xanthomonas campestris pv. Campestris B100 Culture Grown in Minimal Medium with a Specific Focus on Nutrient Consumption and Xanthan Biosynthesis
Abstract
:1. Introduction
2. Materials and Methods
2.1. Cultivation of Xcc
2.2. Xcc Harvesting
2.3. Xanthan Gum, Glucose, and Nitrate Determination
2.4. Whole-Cell Protein Isolation and Digestion
2.5. Sample Preparation for LC-MS/MS Analysis
2.6. LC-MS/MS Analysis
2.7. Data Analysis
3. Results
3.1. Nutrient Consumption and Xanthan Production of Suspension Cultures of Xcc B100 over a Time Course
3.2. The Abundance of Ribosomal Proteins Varies Per Growth Phase
3.3. The Effect of Nitrate Depletion on the Abundance of Proteins Involved in Nitrogen Metabolism
3.4. Proteins Involved in the Synthesis of Sugar Nucleotide Precursors and Xanthan
4. Discussion
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Conflicts of Interest
References
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Struck, B.; Wiersma, S.J.; Ortseifen, V.; Pühler, A.; Niehaus, K. Comprehensive Proteome Profiling of a Xanthomonas campestris pv. Campestris B100 Culture Grown in Minimal Medium with a Specific Focus on Nutrient Consumption and Xanthan Biosynthesis. Proteomes 2024, 12, 12. https://doi.org/10.3390/proteomes12020012
Struck B, Wiersma SJ, Ortseifen V, Pühler A, Niehaus K. Comprehensive Proteome Profiling of a Xanthomonas campestris pv. Campestris B100 Culture Grown in Minimal Medium with a Specific Focus on Nutrient Consumption and Xanthan Biosynthesis. Proteomes. 2024; 12(2):12. https://doi.org/10.3390/proteomes12020012
Chicago/Turabian StyleStruck, Ben, Sanne Jitske Wiersma, Vera Ortseifen, Alfred Pühler, and Karsten Niehaus. 2024. "Comprehensive Proteome Profiling of a Xanthomonas campestris pv. Campestris B100 Culture Grown in Minimal Medium with a Specific Focus on Nutrient Consumption and Xanthan Biosynthesis" Proteomes 12, no. 2: 12. https://doi.org/10.3390/proteomes12020012