Overexpression of MsDREB1C Modulates Growth and Improves Forage Quality in Tetraploid Alfalfa (Medicago sativa L.)
Abstract
:1. Introduction
2. Results
2.1. Msa0891980 Encodes a DREB1C in Alfalfa
2.2. MsDREB1C Responses to Multiple Abiotic Stresses
2.3. Nuclear Protein MsDREB1C Has Self-Transactivation Activity
2.4. Overexpression of MsDREB1C in Alfalfa Affects Plant Growth
2.5. Overexpression of MsDREB1C Reduced Gibberellin (GA) Synthesis
2.6. Overexpression of MsDREB1C in Alfalfa Improves Forage Quality by Hindering Lignification in Stem
3. Discussion
4. Materials and Methods
4.1. Plant Material and Growth Conditions
4.2. Total RNA Isolation, Reverse Transcription, and Quantitative RT-PCR Analysis
4.3. Plasmid Construction
4.4. Bioinformatic Analysis
4.5. Plant Transformation and Infiltration
4.6. Self-Transactivation Assay in Yeast
4.7. Measurement of Growth-Related Indicators
4.8. Measurement of Gibberellin (GA) Content
4.9. Lignification Analysis
4.10. Forage Quality Measurement
4.11. Statistical Analysis
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Data Availability Statement
Conflicts of Interest
References
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Zhang, Y.; Wang, Z.; Zhang, F.; Wang, X.; Li, Y.; Long, R.; Li, M.; Li, X.; Wang, Q.; Yang, Q.; et al. Overexpression of MsDREB1C Modulates Growth and Improves Forage Quality in Tetraploid Alfalfa (Medicago sativa L.). Plants 2024, 13, 1237. https://doi.org/10.3390/plants13091237
Zhang Y, Wang Z, Zhang F, Wang X, Li Y, Long R, Li M, Li X, Wang Q, Yang Q, et al. Overexpression of MsDREB1C Modulates Growth and Improves Forage Quality in Tetraploid Alfalfa (Medicago sativa L.). Plants. 2024; 13(9):1237. https://doi.org/10.3390/plants13091237
Chicago/Turabian StyleZhang, Yangyang, Zhen Wang, Fan Zhang, Xue Wang, Yajing Li, Ruicai Long, Mingna Li, Xianyang Li, Quanzhen Wang, Qingchuan Yang, and et al. 2024. "Overexpression of MsDREB1C Modulates Growth and Improves Forage Quality in Tetraploid Alfalfa (Medicago sativa L.)" Plants 13, no. 9: 1237. https://doi.org/10.3390/plants13091237