Functional Characterization of the MeSSIII-1 Gene and Its Promoter from Cassava
Abstract
:1. Introduction
2. Results
2.1. Isolation and Characterization of MeSSIII-1
2.2. Subcellular Localization of MeSSIII-1 Protein
2.3. Expression Patterns of MeSSIII-1 in Cassava Tissues
2.4. Isolation and Cis-Element Analysis of MeSSIII-1 Promoter
2.5. Expression Profiles of MeSSIII-1 Gene under Hormone Treatments
2.6. Analysis of MeSSIII-1 Promoter Activity
2.7. Histochemical Localization of proMeSSIII-1 in Cassava
2.8. Activities of MeSSIII-1 Promoter in Response to MeJA, ABA, and ET Hormones
3. Discussion
4. Materials and Methods
4.1. Plant Materials and Growth Conditions
4.2. Bioinformatics Analysis of the MeSSIII-1 Gene and Promoter
4.3. Cloning of MeSSIII-1 Gene and Promoter of Cassava
4.4. Subcellular Localization of MeSSIII-1 Protein
4.5. Expression Pattern of MeSSIII-1 Gene
4.6. Construction of Truncated MeSSIII-1 Promoter Vectors and Tobacco Leaves Injection
4.7. GUS Staining and Determination of GUS Activity
4.8. Genetic Transformation and Identification of proMeSSIII-1 Promoter in Cassava
4.9. Hormone Treatments in proMeSSIII-1 Transgenic Cassava
4.10. Statistical Analysis
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Cis-Elements | Number | Function | Core Sequence |
---|---|---|---|
ABRE | 1 | cis-acting element involved in the abscisic acid responsiveness | ACGTG |
ERE | 6 | ethylene-responsive element | ATTT TAAA |
CGTCA-motif | 2 | cis-acting regulatory element involved in the MeJA-responsiveness | CGTCA |
TGACG-motif | 2 | cis-acting regulatory element involved in the MeJA-responsiveness | TGACG |
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Lu, X.-H.; Wang, Y.-J.; Zhen, X.-H.; Yu, H.; Pan, M.; Fu, D.-Q.; Li, R.-M.; Liu, J.; Luo, H.-Y.; Hu, X.-W.; et al. Functional Characterization of the MeSSIII-1 Gene and Its Promoter from Cassava. Int. J. Mol. Sci. 2024, 25, 4711. https://doi.org/10.3390/ijms25094711
Lu X-H, Wang Y-J, Zhen X-H, Yu H, Pan M, Fu D-Q, Li R-M, Liu J, Luo H-Y, Hu X-W, et al. Functional Characterization of the MeSSIII-1 Gene and Its Promoter from Cassava. International Journal of Molecular Sciences. 2024; 25(9):4711. https://doi.org/10.3390/ijms25094711
Chicago/Turabian StyleLu, Xiao-Hua, Ya-Jie Wang, Xing-Hou Zhen, Hui Yu, Mu Pan, Dong-Qing Fu, Rui-Mei Li, Jiao Liu, Hai-Yan Luo, Xin-Wen Hu, and et al. 2024. "Functional Characterization of the MeSSIII-1 Gene and Its Promoter from Cassava" International Journal of Molecular Sciences 25, no. 9: 4711. https://doi.org/10.3390/ijms25094711