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Genes
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Genomics and Bioinformatics in Microbial Science
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Genomics and Bioinformatics in Microbial Science

A special issue of Genes (ISSN 2073-4425). This special issue belongs to the section "Microbial Genetics and Genomics".

Deadline for manuscript submissions: 25 October 2024 | Viewed by 450

Special Issue Editor

Dr. Vagner Fonseca

E-Mail Website
Guest Editor
Department of Exact and Earth Sciences, University of the State of Bahia, Salvador 41150-000, Brazil
Interests: bioinformatics; viral evolution; molecular epidemiology; mathematical models; phylogenetic and phylodynamic trees
Special Issues, Collections and Topics in MDPI journals

Special Issue Information

Dear Colleagues,

Genomic research has been pivotal in uncovering the microbial world and shedding light on its evolution, diversity and functionality. We are excited to announce this Special Issue of Genes aiming to untangle genetic intricacies in microorganisms, with a specific focus on bioinformatics advancements.

Bioinformatics tools are crucial in functional genomics, analysing gene expression and regulatory networks in microbial communities. Coupling metagenomics with bioinformatics allows for the exploration of entire microbial ecosystems, unveiling their functional potential and diversity.

We welcome original research, review and methodological papers on bioinformatics applications in microbial research, microbial evolution genomics and epidemiological investigations in the microbial world.

Dr. Vagner Fonseca
Guest Editor

Manuscript Submission Information

Manuscripts should be submitted online at www.mdpi.com by registering and logging in to this website. Once you are registered, click here to go to the submission form. Manuscripts can be submitted until the deadline. All submissions that pass pre-check are peer-reviewed. Accepted papers will be published continuously in the journal (as soon as accepted) and will be listed together on the special issue website. Research articles, review articles as well as short communications are invited. For planned papers, a title and short abstract (about 100 words) can be sent to the Editorial Office for announcement on this website.

Submitted manuscripts should not have been published previously, nor be under consideration for publication elsewhere (except conference proceedings papers). All manuscripts are thoroughly refereed through a single-blind peer-review process. A guide for authors and other relevant information for submission of manuscripts is available on the Instructions for Authors page. Genes is an international peer-reviewed open access monthly journal published by MDPI.

Please visit the Instructions for Authors page before submitting a manuscript. The Article Processing Charge (APC) for publication in this open access journal is 2600 CHF (Swiss Francs). Submitted papers should be well formatted and use good English. Authors may use MDPI's English editing service prior to publication or during author revisions.

Keywords

  • genomic research
  • microbial genetics
  • bioinformatics
  • functional genomics
  • metagenomics
  • microbial evolution

Published Papers (1 paper)

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Research

14 pages, 3937 KiB  
Article
Genetic Analysis of the ts-Lethal Mutant Δpa0665/pTS-pa0665 Reveals Its Role in Cell Morphology and Oxidative Phosphorylation in Pseudomonas aeruginosa
by Jiayin Zhu, Hulin Zhao and Zhili Yang
Genes 2024, 15(5), 590; https://doi.org/10.3390/genes15050590 - 7 May 2024
Viewed by 364
Abstract
Pa0665 in Pseudomonas aeruginosa shares homologous sequences with that of the essential A-type iron–sulfur (Fe-S) cluster insertion protein ErpA in Escherichia coli. However, its essentiality in P. aeruginosa and its complementation with E. coli erpA has not been experimentally examined. To fulfill this [...] Read more.
Pa0665 in Pseudomonas aeruginosa shares homologous sequences with that of the essential A-type iron–sulfur (Fe-S) cluster insertion protein ErpA in Escherichia coli. However, its essentiality in P. aeruginosa and its complementation with E. coli erpA has not been experimentally examined. To fulfill this task, we constructed plasmid-based ts-mutant Δpa0665/pTS-pa0665 using a three-step protocol. The mutant displayed growth defects at 42 °C, which were complemented by expressing ec.erpA. Microscopic observations indicated a petite cell phenotype for Δpa0665/pTS-pa0665 at 42 °C, correlated with the downregulation of the oprG gene. RNA sequencing revealed significant transcriptional changes in genes associated with the oxidative phosphorylation (OXPHOS) system, aligning with reduced ATP levels in Δpa0665/pTS-pa0665 under 42 °C. Additionally, the ts-mutant showed heightened sensitivity to H2O2 at 42 °C. Overall, our study demonstrates the essential role of pa0665 for OXPHOS function and is complemented by ec.erpA. We propose that the plasmid-based ts-allele is useful for genetic analysis of essential genes of interest in P. aeruginosa. Full article
(This article belongs to the Special Issue Genomics and Bioinformatics in Microbial Science)
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