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Article

Synthesis and Natural Distribution of Anti-inflammatory Alkamides from Echinacea

1
Iowa State University Department of Chemistry, Ames, IA 50011, USA
2
Iowa State University Department of Genetics, Development and Cell Biology, Ames, IA 50011, USA
*
Author to whom correspondence should be addressed.
Molecules 2006, 11(10), 758-767; https://doi.org/10.3390/11100758
Submission received: 2 September 2006 / Revised: 10 October 2006 / Accepted: 16 October 2006 / Published: 18 October 2006

Abstract

:
The synthesis of the alkamides 2Z,4E-undeca-2,4-dien-8,10-diynoic acid isobutyl amide (1) and 2Z,4E-undeca-2,4-dien-8,10-diynoic acid isobutyl amide (5) was accomplished by organometallic coupling followed by introduction of the doubly unsaturated amide moiety. The distribution of these two amides in accessions of the nine species of Echinacea was determined. 

Introduction

Echinacea angustifolia, Echinacea pallida and Echinacea purpurea are the main medicinal Echinacea species and have long been used to treat infections, to aid in wound healing and to enhance the immune system [1]. In 2005, Echinacea products ranked among the top botanical supplements sold in the United States. In recent years, treatment of rhinoviruses has been the focus of several studies, a number of which have failed to show the efficacy of Echinacea [2]. Commercial Echinacea products often are mixtures of the three main medicinal species and there is no regulation of the concentrations of the chemical constituents. Among the chemical constituents of Echinacea species, the alkamides, caffeic acid derivatives such as chicoric acid and the polyphenols are considered important for biological activity [3].
Alkamide levels differ significantly among roots, stems, and flowers of E. purpurea. The roots had higher levels of the dodeca-2,4-diene-8,10-diyne alkamides, whereas levels of the dodecatetraene alkamides and nonadeca-2,4-diene-8,10-diynes were highest in stems [4]. Accessions from different geographical regions often show different chemical fingerprints. Additionally, preliminary studies on the stability of alkamide compounds in E. angustifolia revealed a 13% loss of alkamide levels over two months [5]. The effects of storage time and temperature on alkamide levels in E. purpurea roots showed that levels of all alkamides fell by over 80% during storage at 24° C for 64 weeks [6]. The ready availability of authentic standards of select alkamides would not only facilitate standardization for the purposes of medical studies, but would also permit biological evaluation of individual components.
The recent discovery that dodecadiendiynoic amide 1 from E. purpurea and E. pallida inhibited LPS-mediated activation of a murine macrophage line, RAW264.7, suggests that this alkamide may have anti-inflammatory activity [7].
Molecules 11 00758 i001
Using male rats, an in vivo study examined the immunomodulatory effects of alkamides purified from Echinacea purpurea. These results suggest that the alkamides are among the active constituents of E. purpurea plants. At a dose level of approximately 12 μg/kg body weight/day they effectively stimulated alveolar macrophage function in healthy rats [8]. Alkamides isolated from Echinacea angustifolia had inhibitory activity in in vitro cyclooxygenase (sheep microsomes) and 5-lipoxygenase (porcine leukocytes) assays [9]. Hexane extracts of Echinacea variably inhibit growth of yeast strains of Saccharomyces cerevisiae, Candida shehata, C. albicans, and C. tropicalis under near UV irradiation and to a lesser extent without irradiation [10]. Synergistic antioxidant effects were found when cichoric acid was combined with a natural mixture of alkamides [11].

Results and Discussion

Synthesis of alkamides

Despite the potential importance of the alkamides, few reports of synthesis of authentic standards have been reported. Crombie and co-workers have reported elegant syntheses of natural amides using organometallic coupling reactions [12]. Bohlmann synthesized 1 and 5 in low overall yields, in part because the Wittig reactions that installed the cis-amide moiety produced the cis-isomers in only 13-14% yields [13]. Kraus and Bae have reported syntheses of amides 2 and 3 and ketone 4 [14,15]. We report herein the preparation of diacetylenic amides 1 and 5. The improved overall yields in our syntheses are the result of recent advances in synthetic methodology and the ready availability of larger quantities of these bioactive amides should accelerate the study of the scope of activity of these novel compounds.
Molecules 11 00758 i002
The general procedure for synthesis of amides 1 and 5 is shown in Scheme 1 below. Copper chloride-mediated coupling of 6 [16] followed by oxidation and a Wittig reaction provide 7a or 7b. Reduction of the ester to an aldehyde with DIBAL followed by Swern oxidation and a cis-selective Wittig reaction [17] generated 1 in 38 % overall yield from 7b. Removal of the silyl group with tetra-n-butylammonium fluoride afforded 5 in 45 % overall yield from 7a.
Scheme 1.
Scheme 1.
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Characterization and distribution of the diacetylenic isobutylamides in Echinacea

The distribution of these two amides in accessions of the nine species of Echinacea (E. angustifolia, E. purpurea, E. pallida, E. sanguinea, E. simulata, E. tennesseensis, E. atrorubens, E. laevigata and E. paradoxa) was determined by HPLC. Amides 1 and 5 elute at 19.55 min and 23.28 min respectively. These two amides display very similar UV spectra, all with an absorption maximum at 260 nm, which agrees with that reported [3].
Figure 1. Representative HPLC chromatogram obtained from a 95% ethanolic extract of 6-month-old E. purpurea roots, indicating amides 1 and 5.
Figure 1. Representative HPLC chromatogram obtained from a 95% ethanolic extract of 6-month-old E. purpurea roots, indicating amides 1 and 5.
Molecules 11 00758 g001
The mean levels of amides 1 and 5 in roots, flowers and leaves from 6-month-old plants from nine Echinacea species are presented in Figure 2. These two amides are distributed widely in Echinacea. Our results show that of the nine Echinacea species examined, amide 1 is present in at least six species (E. purpurea, E. pallida, E. sanguinea, E. simulate, E. laevigata and E. paradoxa), while amide 5 is present in at least eight species (E. angustifolia, E. purpurea, E. pallida, E. sanguinea, E. simulata, E. tennesseensis, E. laevigata and E. paradoxa). The highest concentrations of both amide 1 (0.818 ± 0.06 mg g-1 fr. wt) and amide 5 (0.826 ± 0.1mg g-1 fr. wt) were found in E. purpurea roots, which have significantly higher amount of these two amides compared with all other species that contain these compounds. The species that is deficient in both amide 1 and amide 5 is E. atrorubens. Although not typically used for commercial medicinal preparations, some Echinacea species (e.g. E. paradoxa, E. laevigata and E. simulata) have fairly high contents of these amides.
We also found that the abundance of these two amides varied considerably with organ type. Generally speaking, they are present mainly in roots, displaying reduced abundance or even being not detectable in flowers and they are not detectable (the limit of HPLC detection for both amides is approximately 0.02 μg mL−1) in leaves. Interestingly, there is one exception, the E. paradoxa species, whose leaves have the highest quantity of amide 5 (0.303 ± 0.052 mg g-1 fr. wt), whereas flowers have less (0.147 ± 0.052 mg g-1 fr. wt ) and roots have the least (0.145 ± 0.019 mg g-1 fr. wt). This indicates that the biosyntheses of these amides may be regulated differently in different organs and species.

Conclusions

We have presented an improved synthesis of diacetylenic amides 1 and 5 which should pove useful for the preparation of appropriate standards. Investigation of their distribution in different Echinacea species revealed that both genetic source and organ type of the plant materials used in Echinacea preparations need to be considered for product standardization. The information reported in this work will be very useful for the standardization of Echinacea products.
Figure 2. Concentration of amides 1 and 5 in roots (A), flowers (B), and leaves (C) from 6-month-old plants of nine species of Echinacea. Error bars indicate standard deviations of means of triplicate experiments. For each amide, different letters (a - g) indicate a significant difference (p < 0.05).
Figure 2. Concentration of amides 1 and 5 in roots (A), flowers (B), and leaves (C) from 6-month-old plants of nine species of Echinacea. Error bars indicate standard deviations of means of triplicate experiments. For each amide, different letters (a - g) indicate a significant difference (p < 0.05).
Molecules 11 00758 g002

Experimental

General

Unless stated otherwise, all reactions were magnetically stirred and monitored by thin-layer chromatography (TLC) using 0.25 mm precoated silica gel F254 plates (Sigma-Aldrich). Column or flash chromatography were performed with the indicated solvents using silica gel (230-400 mesh) purchased from Dynamic Adsorbents, LLC. All melting points were obtained on a Laboratory Devices capillary melting point apparatus and are uncorrected. 1H- and 13C-NMR spectra were recorded on a Bruker VXR-300 (300 MHz) or a Bruker VXR-400 (400 MHz) spectrometer. Chemical shifts are reported relative to internal chloroform (1H, 7.26 ppm; 13C, 77.23 ppm). High resolution mass spectra were performed at the Iowa State University Mass Spectrometry Laboratory.

Plant material and extraction

Nine species of Echinacea, E. angustifolia (Accession 631267), E. purpurea (Accession 631307), E. pallida (Accession 631293), E. sanguinea (Accession A23878), E. simulata (Accession 631249), E. tennesseensis (Accession 631325), E. atrorubens (Accession 631262), E. laevigata (Accession 631312) and E. paradoxa (Accession 631301), provided by Dr. Mark P. Widrlechner at the USDA-ARS North Central Regional Plant Introduction Station, were studied to evaluate the natural distribution of amides 1 and 5 in Echinacea species. Six-month-old roots, flowers and leaves from each species/accessions were used. Specific plant growth conditions, plant material harvest and extraction method are the same as those in our previously published work [15]. 7-Hydroxy-(E)-N-isobutylundeca-2-ene-8,10-diynamide (C15H21O2) was added as an internal standard prior to extraction for quantification purposes. All experiments were performed in triplicate on independently extracted plant samples from three individual plants.

HPLC analysis

Ethanol extract (15 μL) was injected into a YMC-Pack ODS-AM RP C18 (250 x 4.6 mm, 5 μm) column (Waters, MA) on a Beckman Coulter HPLC equipped with a 508 autosampler, 126 pump control and 168 UV-photodiode array detector (PDA) controlled by 32karat TM software (Version 5.0). The solvent system used was CH3CN/H2O at a flow rate of 1.0 mL/min following a linear gradient of 40→80% CH3CN in H2O over 45 min. Online UV spectra were collected between 200–400 nm. Compound quantification was carried out by calculating the UV response relative to the internal standard 7-hydroxy-(E)-N-isobutylundeca-2-ene-8,10-diynamide (C15H21O2), which has been found suitable for use as an internal standard for these two amides because it was not found in Echinacea plants and does not overlap with any other metabolites found in Echinacea. Amides 1 and 5 were quantified at UV 260 nm with respect to the internal standard, using relative response factors to correct for absorbance differences between these two amides and the standard. These relative response factors for amides 1 and 5 were calculated at UV 260 nm. Various amount of authentic amide 1 or amide 5 (0.625 – 3.125 µg) with internal standard (2.5 µg) were injected to give average relative response factors of 0.0677 (R2 = 0.99) for amide 1, and 0.0669 (R2 = 0.99) for amide 5, respectively. The internal quantification method used here can account for variations in extraction efficiencies in different extracts. The HPLC detection limit for both of the amides was approximately 0.02 μg mL−1.

Statistical analysis

Statistical analyses were performed using SAS software version 8.02 (SAS Institute Inc., Cary, NC). One-way analysis of variance followed by the Tukey test was used to compare means. Significance of difference was defined at p < 0.05.

Ethyl 9-trimethylsilylnona-2-ene-6,8-diynoate (7a).

To a solution of trimethylsilylacetylene (0.5 mL, 3.51 mmol) and 5-iodo-4-pentynol 6 (0.281 g, 1.34 mmol) in degassed piperidine (2 mL) was added CuCl (0.014 g, 0.14 mmol) at 0 oC. The mixture was stirred at rt for 0.5 h and then quenched with sat. NH4Cl (aq) (6 mL) and extracted with Et2O (3 x 10 mL). The combined organic layers were washed with brine (2 x 20 mL), dried (MgSO4), filtered and concentrated. The crude residue was purified via flash chromatography to give the alcohol (0.188 g, 78 % yield).
Dimethylsulfoxide (0.766 mL, 10.8 mmol) was added dropwise at -78 oC to a solution of oxalyl chloride (0.471 mL, 5.4 mmol) in CH2Cl2 (10 mL). The mixture was stirred at same temp. for 20 min and triethylamine (2.25 mL, 16.2 mmol) was added dropwise and stirred at same temp. for 20 min. To the mixture was added the alcohol synthesized above (0.487 mg, 2.7 mmol) at -78 oC and stirred for 80 min while slowly warmed to room temperature. The reaction was quenched with sat NH4Cl (aq) and the aqueous layer was extracted with CH2Cl2 (2 x 20 mL). The combined organic layers were washed with water (2 x 10 mL), dried (MgSO4), filtered and concentrated in vacuo. The crude residue was purified via flash column chromatography to give the aldehyde (0.409 g, 85 % yield).
To a solution of carbethoxymethyl(triphenyl)phosphonium bromide (3.94 g, 9.19 mmol) in THF (30 mL) was added n-BuLi (3.67 mL, 2.5 M soln in hexane) at 0 oC under Ar. The mixture was stirred for 20 min at 0 oC and added the above aldehyde (0.409 g, 2.29 mmol) at the same temperature. After 1 h of stirring at room temperature, reaction was quenched with sat. NH4Cl (aq) and extracted with Et2O (3x30 mL), dried (MgSO4), filtered and concentrated in vacuo. The crude residue was purified via flash column chromatography to give compound 7a (0.465 g, 82% yield). 1H-NMR (300 MHz, CDCl3) δ 6.94-6.89(m, 1H), 5.86 (d, J= 15.2 Hz, 1H), 4.17 (q, J= 7.2 Hz, 2H), 2.43 (m, 4H), 1.28 (t, J= 7.2 Hz, 3H), 0.18 (s, 9H).

2Z,4E-undeca-2,4-dien-8,10-diynoic acid isobutyl amide (5).

To a solution of compound 7a (0.341 g, 1.37 mmol) in 10 mL of THF was added DIBAL (4.12 mL, 1 M soln) at -78 oC in Ar. After stirring for 2 h at -78 oC, the reaction was quenched with EtOAc (30 mL) at -78 oC and the reaction wixture was warmed to rt, washed with 10% HCl(aq), brine, dried (MgSO4), filtered and concentrated in vacuo. The crude residue was purified via flash chromatography to give allyl alcohol (0.260 g, 92 % yield)
Dimethylsulfoxide (0.178 mL, 2.46 mmol) was added dropwise at -78 oC to a solution of oxalyl chloride (0.110 mL, 1.23 mmol) in CH2Cl2 (5 mL). The mixture was stirred at the same temperature for 20 min and triethylamine (0.526 mL, 3.69 mmol) was added dropwise and stirred at same temperature for 20 min. To the mixture was added the above alcohol (0.127 mg, 0.616 mmol) at -78 oC and stirred for 80 min while slowly warming to rt. The reaction was quenched with sat NH4Cl (aq) and aqueous layer was extracted with CH2Cl2 (2x 10 mL). The combined organic layers were washed with water (2x 10 mL), dried (MgSO4), filtered and concentrated in vacuo. The crude residue was purified via flash column chromatography to give aldehyde (0.106 g, 81% yield.)
To a solution of diphenylphosphonoacetamide (0.187 g, 0.539 mmol) in 10 mL of THF was added NaHMDS (0.735 mL, 1M soln in THF) at -78 oC and stirred at same temperature for 20 min. To the mixture was added above aldehyde (0.1 g, 0.49 mmol) in THF (2 mL) by cannula and the resulting mixture was warmed to 10 oC over 2 h. The reaction was quenched with NH4Cl (aq), washed with water, brine, dried (MgSO4), filtered and concentrated in vacuo. The crude residue was purified via flash column chromatography to give (2Z, 4E) amide (0.090 g, 62 % yield). 1H-NMR (300 MHz, CDCl3) δ 7.49 (dd, J= 15.3, 11.4 Hz, 1H), 6.37 (t, J= 11.4 Hz, 1H), 6.05-5.90 (m, 1H), 5.58 (brs, 1H), 5.52 (d, J= 12.9 Hz, 1H), 3.12 (t, J= 6.9 Hz, 2H), 2.39-2.38 (m, 4H), 1.84-1.75 (m, 1H), 0.92 (d, J= 6.9 Hz, 6H), 0.18 (s, 9H); 13C-NMR (75 MHz, CDCl3) δ 166.5, 140.9, 140.0, 128.5, 119.9, 88.5, 82.3, 79.1, 66.2, 46.9, 31.6, 28.8, 20.4, 19.4, -0.13; HRMS m/e (EI) for C18H27NOSi (M)+ calcd 301.1862, measured 301.1843
To a solution of the above (2Z, 4E) amide (0.032 g, 0.106 mmol) in THF (2 mL) was added TBAF (0.159 mL, 1.159 mmol) at 0 oC. The mixture was stirred for 1h at rt and the solvent was removed. The crude residue was purified via flash column chromatography to give compound 5 (0.024 g, 99 % yield) 1H-NMR (300 MHz, CDCl3) δ 7.51 (dd, J= 14.7, 11.4 Hz, 1H), 6.37(t, J= 11.4 Hz, 1H), 6.02-5.89 (m, 1H), 5.63 (brs, 1H), 5.53 (d, J= 11.4 Hz, 1H), 3.12 (t, J= 6.6 Hz, 2H), 2.49-2.31 (m, 4H), 1.97 (s, 1H), 1.84-1.74 (m, 1H), 0.91 (d, J= 6.6 Hz, 6H);13C-NMR (75 MHz, CDCl3) δ 166.5, 140.9, 139.8, 128.5, 119.9, 82.3, 77.5, 65.2, 65.1, 46.9, 31.4, 28.8, 20.4, 19.1; HRMS m/e (EI) for C15H19NO (M)+ calcd 229.1467, measured 229.1579.

Ethyl deca-2-ene-6,8-diynoate (7b).

Degassed piperidine (5.5 mL), 5-iodo-4-pentynol (1.74 g, 8.49 mmol) and CuCl (0.086 g, 0.85 mmol) were mixed in a sealed tube. The mixture was cooled to -78 oC and excess propyne gas (condensed to liquid, 2 mL) was added by blowing along the wall of the tube. The mixture was slowly warmed to room temperature. After stirring for 2 h at rt, the mixture was cooled to -78 oC and the sealed tube was opened then slowly warmed to rt to evaporate excess propyne. NH4Cl (aq) (20 mL) was added to the mixture then extracted with Et2O (3 x 20 mL). The organic layer was washed with water, brine, dried (MgSO4), filtered and concentrated in vacuo. The crude residue was purified via flash column chromatography to give alcohol (0.847 g, 82 % yield)
Dimethylsulfoxide (1.63 mL, 22.9 mmol) was added dropwise at -78 oC to a solution of oxalyl chloride (1 mL, 11.5 mmol) in 60 mL of CH2Cl2. The mixture was stirred at the same temperature for 20 min and triethylamine (4.78mL, 34.4mmol) was added dropwise and stirred at same temperature for 20 min. To the mixture was added above alcohol (0.70 g, 5.73 mmol) at -78 oC and stirred for 80 min while slowly warmed to rt. The reaction was quenched with sat NH4Cl (aq) (10 mL) and aqueous layer was extracted with CH2Cl2 (2x 30 mL). The combined organic layers were washed with water (2x 20 mL), dried (MgSO4), filtered and concentrated in vacuo. The crude residue was purified via flash column chromatography to give aldehyde (0.55 g, 80 % yield). 1H-NMR (300 MHz, CDCl3) δ 9.76 (s, 1H), 2.68 (t, J= 6.6 Hz, 2H), 2.54 (t, J=6.6 Hz, 2H), 1.89 (s, 3H); 13C-NMR (75 MHz, CDCl3) δ 199.9, 74.4, 74.2, 66.5, 64.4, 42.4, 12.6, 4.3.
To a solution of carbethoxymethyl(triphenyl)phosphonium bromide (5.26 g, 12.37 mmol) in THF (40 mL) was added n-BuLi (4.95 mL, 2.5 M soln in hexane) at 0 oC under Ar. The mixture was stirred for 20 min at 0 oC and the aldehyde (0.59 g, 4.95 mmol) was added at same temperature. After 1 h of stirring at rt, the reaction was quenched with sat NH4Cl (aq) and extracted with ethyl ether (3x30 mL), dried (MgSO4), filtered and concentrated in vacuo. The crude residue was purified via flash column chromatography to give compound 7b (0.73 g, 78 % yield). 1H-NMR (300 MHz, CDCl3) δ 7.01-6.85 (m, 1H), 5.86 (d, J= 15.6Hz, 1H), 4.18 (q, J= 7.2 Hz, 2H), 2.43-2.40 (m, 4H), 1.90 (s, 3H), 1.26 (t, J= 7.2 Hz, 3H).

2Z,4E-undeca-2,4-dien-8,10-diynoic acid isobutyl amide (1).

To a solution of compound 7b (0.437 g, 2.3 mmol) in 20 mL of THF was added DIBAL (4.6 mL,1.0M soln in THF) at -78 oC in Ar. After stirring for 2 h at -78oC, the reaction was quenched with ethyl acetate (30 mL) at -78 oC and warmed to rt. The mixture was washed with 10% HCl (aq) (10 mL), brine, dried (MgSO4), filtered and concentrated in vacuo. The crude residue was purified via flash column chromatography to give the allylic alcohol (0.28 g, 81 % yield).
Dimethylsulfoxide (0.530 mL, 7.48 mmol) was added dropwise at -78 oC to a solution of oxalyl chloride (0.326 mL, 3.74 mmol) in 20 mL of CH2Cl2. The mixture was stirred at the same temp for 20 min and triethylamine (1.56 mL, 11.2 mmol) was added dropwise and stirred at same temperature for 20 min. The above alcohol (0.277 g, 1.87 mmol) was added to the mixture at -78 oC and stirred for 80 min while slowly warming to rt. The reaction was quenched with sat NH4Cl (aq) and aqueous layer was extracted with CH2Cl2 (2x 10 mL). Combined organic layer was washed with water (2x 10 mL), dried (MgSO4), filtered and concentrated in vacuo. The crude residue was purified via flash column chromatography to give aldehyde (0.229 g, 84 % yield). 1H-NMR (300 MHz, CDCl3) δ 9.49 (d, J= 7.8 Hz, 1H), 6.83 (dt, J= 15.6, 6.0 Hz, 1H), 6.14 (dd, J= 15.6, 7.8 Hz, 1H), 2.58-2.40 (m, 4H), 1.86 (s, 3H); 13C-NMR (75 MHz, CDCl3) δ 193.9, 155.4, 134.0, 74.3, 74.3, 67.0, 60.6, 31.4, 18.2, 4.3.
To a solution of diphenylphosphonoacetamide (0.370 g, 1.06 mmol) in THF (10 mL) was added NaHMDS (1.06 mL, 1 M soln in THF) at -78 oC and stirred at same temp for 20 min. To the mixture was added the above aldehyde (0.140 g, 0.97 mmol) in THF (2 mL) via cannula and the resulting mixture was warmed to 10 oC over 2h. The reaction was quenched with NH4Cl (aq), washed with water, brine, dried (MgSO4), filtered and concentrated in vacuo. The crude residue was purified via flash column chromatography to give amide 1 (0.131g, 56% yield). 1H-NMR (300 MHz, CDCl3) δ 7.47 (dd, J= 15.3, 11.4 Hz, 1H), 6.34 (t, J= 11.4 Hz, 1H), 5.99-5.87 (m, 1H), 5.78 (brs, 1H), 5.52 (d, J= 11.4 Hz, 1H), 3.09 (t, J= 6.6 Hz, 2H), 2.37-2.32 (m, 4H), 1.87 (s, 3H), 1.82-1.73 (m, 1H), 0.89 (d, J= 6.6 Hz, 6H); 13C-NMR (75 MHz, CDCl3) δ 166.7,140.9, 140.3, 128.4, 119.9, 75,8, 73,7, 66.2, 64.7, 46.9, 31.8, 28.8, 20.4, 19.3, 4.4.

Acknowledgments

We thank the National Institutes of Health (grant P01 ES12020) and the Office of Dietary Supplements for partial financial support through the Center for Research on Botanical Dietary Supplements at Iowa State University.

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  • Sample availability: Milligram quantities of compounds 1 and 5 are available from the authors.

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MDPI and ACS Style

Kraus, G.A.; Bae, J.; Wu, L.; Wurtele, E. Synthesis and Natural Distribution of Anti-inflammatory Alkamides from Echinacea. Molecules 2006, 11, 758-767. https://doi.org/10.3390/11100758

AMA Style

Kraus GA, Bae J, Wu L, Wurtele E. Synthesis and Natural Distribution of Anti-inflammatory Alkamides from Echinacea. Molecules. 2006; 11(10):758-767. https://doi.org/10.3390/11100758

Chicago/Turabian Style

Kraus, George A., Jaehoon Bae, Lankun Wu, and Eve Wurtele. 2006. "Synthesis and Natural Distribution of Anti-inflammatory Alkamides from Echinacea" Molecules 11, no. 10: 758-767. https://doi.org/10.3390/11100758

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