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Article

Synthesis and Phytotoxic Activity of New Pyridones Derived from 4-Hydroxy-6-Methylpyridin-2(1H)-one

by
Antonio Jacinto Demuner
1,
Vania Maria Moreira Valente
1,
Luiz Cláudio Almeida Barbosa
1,2,*,
Akshat Rathi
2,
Timothy J. Donohoe
2 and
Amber L. Thompson
2
1
Department of Chemistry, Federal University of Vicosa, Av. P. H. Rolfs, s/n, 36570-000, Vicosa, MG, Brazil
2
Department of Chemistry, University of Oxford, 12 Mansfield Road, Oxford OX1 3TA, UK
*
Author to whom correspondence should be addressed.
Molecules 2009, 14(12), 4973-4986; https://doi.org/10.3390/molecules14124973
Submission received: 5 November 2009 / Revised: 24 November 2009 / Accepted: 30 November 2009 / Published: 1 December 2009
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Abstract

:
Commercial dehydroacetic acid was converted into 4-hydroxy-6-methylpyridin-2(1H)-one (3), which was then condensed with several aliphatic aldehydes to produce seven new title compounds in variable yields (35–92%). Reaction of 3 with α,β-unsaturated aldehydes resulted in the formation of condensed pyran derivatives 4g’ and 4h’. A mechanism is proposed to explain the formation of such compounds. The effects of all methylpyridin-2(1H)-one derivatives on the development of the dicotyledonous species Ipomoea grandifolia and Cucumis sativus and the monocotyledonous species Sorghum bicolor were evaluated. At the dose of 6.7 × 10-8 mol a.i./g substrate the compounds showed some phytotoxic selectivity, being more active against the dicotyledonous species. These compounds can be used as lead structures for the development of more active phytotoxic products.

1. Introduction

Pyridin-2(1H)-ones are known to possess a range of biological activities such as analgesic, antifungal, antimalarial, antiinflammatory, antibacterial, anti-HIV, phytotoxic, antitumoral and antiviral properties [1,2,3,4,5,6,7,8,9]. Functionalized pyridin-2(1H)-ones have been used as versatile intermediates in the synthesis of a wide range of nitrogen-containing heterocycles, such as pyridine, quinolizidine, and indolizidine alkaloids [10,11]. Fluridone and sapinopyridione are representative examples of pyridinones that possess phytotoxic activity [5,7]. This class of compounds has been used to formulate lead compounds for the synthesis of new pyridiones [1,3,4,12,13], including the bis(pyridyl)methanes, which present antitumoral activity [8,9]. Recently, we have been utilizing natural products as models for the development of new compounds with phytotoxic activity [14,15,16,17,18,19,20,21,22,23,24]. In continuing our research in this direction, we now report the synthesis of pyridone derivatives 4a-h and some studies on their phytotoxic activities.

2. Results and Discussion

2.1. Synthesis of bis-pyridones

The hydrolysis of dehydroacetic acid 1 [25] with sulfuric acid afforded compound 2 in 86% yield. Compound 2 was then reacted with aqueous ammonium hydroxide to produce the corresponding pyridone 3 in 80% yield. Treatment of 3 under basic conditions in the presence of several aldehydes resulted in the condensed compounds 4a-h in variable yields (Scheme 1).
Molecules 14 04973 g004 550
Scheme 1. Reagents and conditions for the synthesis of pyridones 4a-h.
Scheme 1. Reagents and conditions for the synthesis of pyridones 4a-h.
Molecules 14 04973 g004
It was found that formaldehyde reacted very quickly and efficiently, resulting in a 92% yield of the condensed bis-pyridone 4a. In the cases of acetaldehyde and propanaldehyde the reactions were not clean, giving the desired condensation compounds in much lower yields (4b, 35%; 4c, 56%) and several side products were observed by TLC analysis of the crude reaction mixtures. To study the influence on the biological activity of an unsaturated group at the methine carbon, acrolein was reacted with compound 3 under the same conditions described. In this case the required bis-pyridone was not isolated and only polymerized compounds were detect by ESI-MS and NMR of the crude reaction mixture.
In order to further explore the Structure Activity Relationships (SAR), the preparation of condensed bis-pyridone containing aromatic groups linked to the methine carbon bridge was attempted. Thus, the reaction of benzaldehyde and 2-phenylpropanaldehyde with 3 resulted in the formation of the corresponding bis-pyridones 4e and 4f in 56% and 75% yields, respectively. The structures of all condensed products were determined by spectroscopic analysis. In the case of compound 4f, the structure was also determined by X-ray analysis (Figure 1).
Molecules 14 04973 g001 550
Figure 1. Single crystal structure of compound 4f. Atomic displacement parameters shown at 50% probability and the disorder are omitted for clarity.
Figure 1. Single crystal structure of compound 4f. Atomic displacement parameters shown at 50% probability and the disorder are omitted for clarity.
Molecules 14 04973 g001
Unexpectedly the reaction of pyridione 3 with cinnamaldehyde and (E)-2-methylcinnamaldehyde did not give the desired condensed products. In case of the cinnamaldehyde adduct the HR-MS showed a peak at m/z =387.1301, corresponding to the molecular formula C21H20NaN2O4. The 13C-NMR spectrum showed signals corresponding to two CH3, one CH2, six CH, two C=O and seven non-hydrogenated carbons. For the (E)-2-methylcinnamaldehyde adduct, the product isolated as a white solid with HR-MS showed a peak at m/z = 276.0095 corresponding to the molecular formula C16H15NaNO2. The 13C-NMR spectrum showed signals corresponding to two CH3, six CH, one C=O and five non-hydrogenated carbons. Single crystal diffraction data were collected for both 4g’ and 4h’ yielding the structures shown in Scheme 2, Figure 2, Figure 3. Both compounds were formed as a racemic mixture. Formation of a compound similar to 4h’ has been reported by Lee et al. [26], however in none of the published examples a compound with the structure of 4g’ was observed.
Although no mechanistic investigation was carried out, a pathway is proposed that leads to the formation of 4g’ and 4h’ (Scheme 2). Initially, an aldol condensation of pyridone 3 with cinnamaldehyde results in the intermediate (a). Depending on the nature of the R group (H or CH3) the product formed in the next step is different. In case of cinnamaldehyde a 1,6-addition of a second molecule of the pyridone resulting in the intermediate (c) occurs. This intermediate can be in equilibrium with (d), which under basic conditions cyclises to yield 4g’. In case of (E)-2-methylcinnamaldehyde, steric hindrance involving the methyl group and the oxygen favors the formation of (b). This intermediate then undergoes a 3,3-sigmatropic rearrangement to give 4h’. This rearrangement ensures that a second addition is impossible. At this point we should observe that according to the proposed mechanism a concentration of pyridine could have some influence on the results of the reactions, leading to different products, but this is open to further investigation.
Molecules 14 04973 g005 550
Scheme 2. Proposed mechanism for formation of 4g’ and 4h’.
Scheme 2. Proposed mechanism for formation of 4g’ and 4h’.
Molecules 14 04973 g005
Molecules 14 04973 g002 550
Figure 2. Single crystal structure of compound 4g’. Atomic displacement parameters shown at 50% probability, one equivalent and the disordered DMSO are omitted for clarity.
Figure 2. Single crystal structure of compound 4g’. Atomic displacement parameters shown at 50% probability, one equivalent and the disordered DMSO are omitted for clarity.
Molecules 14 04973 g002
Molecules 14 04973 g003 550
Figure 3. Single crystal structure of 4h’ showing the hydrogen bonding amide dimer. Atomic displacement parameters are shown at 50% probability.
Figure 3. Single crystal structure of 4h’ showing the hydrogen bonding amide dimer. Atomic displacement parameters are shown at 50% probability.
Molecules 14 04973 g003

2.2. Phytotoxicity assay

All the compounds were evaluated against S. bicolor, C. sativus and I. grandifolia, using sand as a substrate. Although the compounds could have different adsorption properties on sand and this could affect the biological activity, this aspect was beyond the scope of the current work. The effect of compounds 4a-c, 4e-f, 4g’ and 4h’ on the radicle growth of Sorghum bicolor and Cucumis sativus was evaluated at 5 × 10-4 mol L-1 concentration (Table 1). All the compounds caused less than 50% inhibition of both tested plants. The most active compounds were 4g’ and 4h’, should be causing around 45% inhibition of the root growth of S. bicolor and C. sativus.
Table
Table 1. Effects of compounds 4a-c and 4e-h (5 × 10-4 mol L-1) on the radicle growth of S. bicolor and C. sativus seedlings after 72 hours.
Table 1. Effects of compounds 4a-c and 4e-h (5 × 10-4 mol L-1) on the radicle growth of S. bicolor and C. sativus seedlings after 72 hours.
CompoundC. sativus inhibition (%)*S. bicolor inhibition (%)
4a27.6 Ca27.1 Ba
4b31.2 BCa23.9 Ba
4c30.2 BCa26.9 Ba
4e 36.5 ABCa18.9 Bb
4f38.5 ABCa25.0 Bb
4g’41.0 ABa44.3 Aa
4h’45.3 Aa44.3 Aa
* Mean values in the same column with the same capital letters are not significant at P = 0.05% by Tukey’s test; Mean values in the same line with the same small letter are not significant at P = 0.05% by Tukey’s test.
Once all compounds showed some activity on the Petri dish test they were submitted to a 21 day assay in a greenhouse using S. bicolor, C. sativus and the weed Ipomoea grandifolia as test plants (Table 2).
Table
Table 2. Effect of compounds 4a-c, e-h (6.7 × 10-8 mol a.i./g substrate) on the biomass production of radicle growth of S. bicolor and C. sativus seedlings after 21 days.
Table 2. Effect of compounds 4a-c, e-h (6.7 × 10-8 mol a.i./g substrate) on the biomass production of radicle growth of S. bicolor and C. sativus seedlings after 21 days.
-S. bicolorC. sativusI. grandifolia
aerial part*rootsaerial partrootsaerial partroots
4a22.3 CDc37.4 ABb42.6 Bb65.0 BCa64.4 Aa38.6 DEb
4b31.8 BCc48.0 Ab63.7 Aa80.8 Aa49.8 Bb45.5 CDb
4c51.6 Ab44.2 Ab63.5 Aa63.9 BCa68.4 Aa72.8 Aa
4e18.1 Db31.7 Bb34.9 BCa67.5 BCa32.8 Ca32.8 Eb
4f40.2 ABa38.6 ABb38.7 BCa57.6 Ca45.6 BCa44.3 CDEb
4g’42.7 ABb46.8 Ac38.6 BCb74.5 ABa56.8 ABa58.2 Bb
4h’38.1 Bb42.8 ABb27.5 Cc63.8 BCa49.3 Ba55.3 BCa
* Mean values in the same column with the same capital letters are not significant at P = 0.05% by Tukey’s test; Mean values in the same line with the same small letter are not significant at P = 0.05% by Tukey’s test.
In the case of S. bicolor compounds 4c, 4f and 4g’ caused higher inhibitory effect on the development of the aerial parts (51.6%, 40.2% and 42.7%, respectively). On the other hand, 4b, 4c, and 4g’ caused greatest root inhibition development (48.0%, 44.2% and 46.8%, respectively). The results observed for compound 4g’ confirm its higher activity found in the Petri dish test in relation to the other compounds.
For C. sativus, a dicotyledonous species, compounds 4b and 4c most severely inhibited the development of the aerial parts (63.7% and 63.5%, respectively). All the other compounds, including the pyrans 4g’ and 4h’, caused less than 50% inhibition on the aerial parts. The development of the roots of C. sativus was most affected by compounds 4b (80.8% inhibition) and 4g’ (74.5%). All the other compounds were also very active against this dicotyledonous species, causing increases inhibition of the development of their roots compared to the aerial parts.
Having confirmed the phytotoxicity of the pyridone derivatives against the monocotyledon and dicotyledonous test species, their effect on the development of the important dicotyledonous weed species I. grandifolia were also evaluated. For this species, compounds 4a, 4c and 4g’ inhibited the development of the aerial part by 64.4%, 68.4% and 56.8%, respectively. Compound 4c was by far the most effective in inhibiting the roots of I. grandifolia 72.8%), followed by compounds 4g’ (58.2% inhibition) and 4h’ (55.3%). All the remaining compounds inhibited the development of the roots of I. grandifolia less than 50%.
The compound 4g’ serendipitously emerged as the most potent inhibitor, showing moderate to high inhibition (38.6% to 74.5%) against all the species tested. Although a clear structure-activity relationship cannot be secured from these data, it is evident that the pyran ring is somehow associated with the activity. It may be concluded that presence of one less pyridone moiety in the molecule does not reduce the phytotoxicity, but rather increases it and that the presence of an aromatic ring at any position (closer or farther from the pyridone ring) encourages higher activity than an alkyl substituent.

3. Experimental

3.1. General

All the chemicals were purchased from Sigma Aldrich (Milwaukee, WI, USA) and used without purification. The 1H- and 13C-NMR spectra were recorded on a Varian Mercury 300 instrument (300 MHz and 75 MHz respectively), using deuterated DMSO as a solvent and tetramethylsilane (TMS) as internal standard (δ = 0). IR spectra were obtained using a Perkin Elmer Paragon 1000 FTIR spectrophotometer, using potassium bromide (1% v/v) disks, scanning from 600 to 4000 cm-1. Low-resolution mass spectra were recorded on a Fisons Platform instrument under ESI conditions. Chemical ionisation accurate mass spectra were recorded on an Apex III FT-ICR-MS spectrometer with high resolution (resolution = 100,000 FWHM). The lock used for calibration was chloropentafluorobenzene. Values quoted are reported as a ratio of mass to charge in Daltons. Melting points are uncorrected and were obtained from MQAPF-301 melting point apparatus (Microquimica, Brazil). Analytical thin layer chromatography analysis was conducted on aluminum backed precoated silica gel plates. Column chromatography was performed over silica gel (60–230 mesh).

3.2. Synthesis

3.2.1. Synthesis of 4-hydroxy-6-methyl-2H-pyran-2-one (2)

Dehydroacetic acid 1 (1 mmol) and 92% sulfuric acid aqueous solution (5 mmol) were placed in a 25 mL flask and the mixture was heated to 130 °C for 10 minutes. While still warm, the mixture was poured into a beaker containing chopped ice. The resulting precipitate formed was filtered off and washed with cold water, leading to the isolation of 4-hydroxy-6-methylpyran-2-one (2) as a white solid in 86% yield. Mp 186.5–187.7 °C; IR: 3,440, 3,097, 2,962, 2,821, 2,733, 2,622, 1,717, 1,657, 1,626, 1,586, 1,540, 1,509, 1,493, 1,344, 1,303, 1,256, 1,191, 1,149, 1,042, 989, 878, 839, 814, 591, 526, and 498 cm-1; 1H-NMR (DMSO-d6) δ: 2.13 (d, 3H, J = 0.3 Hz, CH3), 5.19 (d, 1H, J = 2.1 Hz, H3), 5.93 (m, 1H, H5 ), 11.58 (bs, 1H, OH); 13C-NMR (DMSO-d6) δ: 20.1 (6-CH3), 88.8 (C3), 100.9 (C5), 164.0 (C6), 164.6 (C4), 171.2 (C2); MS, m/z (%) 126 (M+, 33), 111 (15), 98 (70), 85 (33), 69 (100), 55 (25).

3.2.2. Synthesis of 4-hydroxy-6-methylpyridin-2(1H)-one (3)

4-Hydroxy-6-methylpyran-2-one (2, 1 mmol) and 28% ammonium hydroxide (5 mmol) were added under stirring to a 25 mL flask. The mixture was heated to 100 °C for 6 h, after which it was cooled in an ice bath and the resultant white solid was filtered off and washed with cold water to provide 4-hydroxy-6-methylpyridin-2-one (3) in 80% yield; Mp > 300 °C; IR: 3,445, 3,268, 3,091, 2,894, 2,796, 2,716, 1,653, 1,634, 1,603, 1,550, 1,400, 1,381, 1,352, 1,267, 1,232, 1,175, 1,042, 903, 842, 830, 627, 595, and 535 cm-1; 1H-NMR (DMSO-d6) δ: 2.06 (d, 3H, J = 0.3 Hz, 6-CH3), 5.32 (d, 1H, J = 2.1 Hz, H3), 5.58 (m, 1H, H5), 10.97 (bs, 1H, OH), 10.41 (bs, 1H, NH); 13C-NMR (DMSO-d6): δ 19.1 (6-CH3), 96.4 (C3), 99.0 (C5), 146.6 (C6), 165.53 (C2), 168.3 (C4); MS, m/z (%) 125 (M+, 77), 96 (22), 84 (100), 69 (17), 68 (17), 55 (16).

3.2.3. General method of preparation of bis(pyridyl)methanes 4a-h

To a 100 mL round-bottomed flask, 95% ethanol (20 mL), methanol (20 mL), 4-hydroxy-6-methylpyridin-2-one (3, 1.5 mmol), piperidine (15 μL) and pyridine (45 μL) were added. The reaction mixture was kept under stirring for 5 minutes at 25 °C. The corresponding aldehyde (1 mmol) was added and the mixture kept under reflux. On completion of the reaction, as determined by TLC analysis, the volume of solvent was reduced under reduced pressure in a rotatory evaporator and the precipitate formed was filtered and washed with methanol, followed by ethyl ether. This led to the formation of 4a-h in high purity, without need of any further purification.
3,3’-Methylenebis(4-hydroxy-6-methylpyridin-2(1H)-one) (4a). Reflux 1.5 h, White solid; yield: 92%; Mp > 300 °C; IR: 3,445, 3,284, 3,059, 3,023, 2,899, 2,644, 1,640, 1,568, 1,442, 1,389, 1,368, 1,267, 1,196, 1,132, 910, 835, 805, 611, and 546 cm-1; 1H-NMR (DMSO-d6) δ: 2.10 (s, 6H, 6-CH3 and 6’-CH3), 3.41 (s, 2H, H7), 5.86 (s, 2H, H5 and H5’), 11.74 (s, 2H, H1 and H1’), 12.11 (bs, 1H, 4-OH and 4’-OH); 13C-NMR (DMSO-d6) δ: 18.7 (C7), 18.9 (6-CH3 and 6’-CH3), 102.1 (C5 and C5’), 109.1 (C3 and C3’), 144.3 (C6 and C6’), 165.6 (C2 and C2’), 167.0 (C4 and C4’); HRMS (ESI TOF-MS): Calcd. for C13H14N2O4 262.0954; found: 262.0951.
3,3'-(Ethane-1,1-diyl)bis(4-hydroxy-6-methylpyridin-2(1H)-one) (4b). Reflux 90 h, White solid; yield: 35%; Mp > 300 °C; IR: 3,446, 3,264, 2,891, 2,610, 1,644, 1,558, 1,451, 1,390, 1,335, 1,265, 1,198, 1,165, 915, 816, 641, and 553 cm-1; 1H-NMR (DMSO-d6) δ: 1.58 (d, 3H, J = 7.5 Hz, H8), 2.09 (s, 6H, 6-CH3 and 6’-CH3), 4.49 (q, 1H, J = 7.5 Hz, H7), 5.81 (s, 2H, H5 and H5’), 11.63 (s, 2H, H1 and H1’); 13C-NMR (DMSO-d6) δ: 15.3 (C8), 18.7 (6-CH3 and 6’-CH3), 25.3 (C7), 102.7 (C5 and C5’), 112.9 (C3 and C3’), 144.0 (C6 and C6’), 166.5 (C2 and C2’), 166.7 (C4 and C4’); HRMS (ESI TOF-MS): Calcd. for C14H16N2O4 276.1110; found: 276.1110.
3,3´-(Propane-1,1-diyl)bis(4-hydroxy-6-methylpyiridin-2(1H)-one) (4c). Reflux 90 h, White solid; yield 56%; Mp > 300 °C; IR: 3,447, 3,057, 2,955, 2,872, 2,622, 1,637, 1,460, 1,393, 1,361, 1,265, 1,252, 1,193, 835, 817, 624, and 557 cm-1; 1H-NMR (DMSO-d6) δ: 0.73 (t, 3H, J = 7.2 Hz, CH3), 2.03-2.20 (m, 8H, H8, 6-CH3 and 6’-CH3), 4.18 (t, 1H, J = 8.1 Hz, H7), 5.81 (s, 2H, H5 and H5’), 11.60 (s, 2H, H1 and H1’); 13C-NMR (DMSO-d6) δ: 13.9 (C9), 18.7 (6-CH3 and 6’-CH3), 21.4 (C8), 33.4 (C7), 102.7 (C5 and C5’), 111.6 (C3 and C3’), 144.2 (C6 and C6’), 166.1 (C2 and C2’), 166.2 (C4 and C4’); HRMS (ESI TOF-MS): Calcd. for C15H18N2O4 290.1267; found: 290.1263.
3,3'-(Phenylmethylene)bis(4-hydroxy-6-methylpyridin-2(1H)-one) (4e). Reflux 40 h, White solid; yield: 56%; Mp > 300 °C; IR: 3,445, 3,280, 3,062, 2,898, 1,632, 1,453, 1,394, 1,361, 1,284, 1,192, 924, 817, 772, and 583 cm-1; 1H-NMR (DMSO-d6) δ: 2.15 (s, 6H, 6-CH3 and 6’-CH3), 5.87 (bs, 3H, H-7, H5 and H5’), 6.99 (d, 2H, J = 7.5 Hz, H2” and H6”), 7.12 (t, 1H, J1 = J2 = 7.5 Hz, H4”), 7.20 (t, 2H, J = 7.5 Hz, H3” and H5”), 11.69 (s, 2H, H1 and H1’), 12.31 (bs, 1H, 4-OH and 4’-OH); 13C-NMR (75 MHz, DMSO-d6) δ: 18.9 (6-CH3 and 6’-CH3), 34.5 (C7), 102.5 (C5 and C5’), 110.2 (C3 and C3’), 126.0 (C4”), 126.9 (C2” and C6”), 128.5 (C3” and C5”), 139.6 (C1”), 144.8 (C6 and C6’), 167.7 (C2 and C2’), 167.5 (C4 and C4’); HRMS (ESI TOF-MS): Calcd. for C19H18N2O4 338.1267; found: 338.1265.
3,3'-(3-Phenylpropane-1,1-diyl)bis(4-hydroxy-6-methylpyridin-2(1H)-one) (4f). Reflux 120 h, White solid; yield 75%; Mp 292.5–293.4 °C; IR: 3,446, 3,284, 3,058, 3,042, 3,024, 2,905, 2,622, 1,635, 1,559, 1,455, 1,437, 1,396, 1,257, 1,177, 816, 697, and 552 cm-1; 1H-NMR (DMSO-d6) δ: 2.10 (s, 6H, 6-CH3 and 6’-CH3), 2.4 (m, 2H, H8), 2.48 (m, 2H, H9), 4.31 (t, 1H, J = 7.5 Hz, H7), 5.83 (s, 2H, H5 and H5’), 7.06-7.24 (m, 5H, H2”, H3”, H4”, H5” and H6”), 11.65 (s, 2H, NH-1 and NH-1’), 11.86 (s, 2H, 4-OH and 4’-OH); 13C-NMR (DMSO-d6) δ: 18.8 (6-CH3 and 6’-CH3), 30.7 (C8), 30.8 (C7), 35.0 (C9), 102.7 (C5 and C5’), 111.5 (C3 and C3’), 126.3 (C4”), 128.9 (C3” and C5”), 129.0 (C2” and C6”), 137.5 (C1”), 142.5 (C6 and C6’), 167.1 (C2 and C2’), 167.2 (C4 and C4’); HRMS (ESI+) C21H22N2NaO4 (MNa+) required 389.1472, found 389.1479.
(±)-2-(4-Hydroxy-6-methyl-2-oxo-1,2-dihydropyridin-3-yl)-7-methyl-4-phenyl-3,4-dihydro-2H-pyrano-[3,2-c]pyridin-5(6H)-one (4g’). Reflux 140 h, White solid; yield 57%; Mp 254.8–255.6 °C; IR: 3,418, 3,132, 3,058, 2,932, 1,616, 1,579, 1,455, 1,390, 1,363, 1,321, 1,260, 1,181, 1,155, 1,113, 1,039, 971, 872, 821, 765, 701, 663, and 598 cm-1; 1H-NMR (DMSO-d6) δ: 1.66 (d, 1H, J = 13.8 Hz, H8), 2.02 (s, 3H, 6-CH3), 2.09 (s, 3H, 6’-CH3), 3.10 (dt, 1H, J = 13.8, 4.8 Hz, H8’), 4.01 (d, 1H, J = 4.8 Hz, H7), 5.12 (dd, 1H, J = 12.3, 1.8 Hz, H9), 5.62 (s, 1H, H5), 5.65 (s, 1H, H5’), 7.08 (d, 2H, J = 7.2 Hz, H2” and H6”), 7.16 (t, 1H, J = 7.2 Hz, H4”), 7.26 (t, 2H, J = 7.2 Hz, H3” and H5”), 10.44 (s, 1H, 4’-OH); 11.02 (s, 2H, NH-1 and NH-1’); 13C-NMR (DMSO-d6) δ: 19.0 (6-CH3), 19.1 (6’-CH3), 32.5 (C8), 35.4 (C7), 67.2 (C9), 98.5 (C5), 98.7 (C5’), 104.6 (C3), 105.5 (C3’), 126.4 (C4”), 128.6 (C2” and C6”), 128.7 (C3” and C5”), 143.8 (C1”), 146.4 (C6 and C6’), 164.0 (C2), 164.3 (C2’), 164.9 (C4), 166.4 (C4’); HRMS (ESI+) C21H20N2NaO4 (MNa+) required 387.1315, found 387.1301.
(±)-2-(4-Hydroxy-6-methyl-2-oxo-1,2-dihydropyridin-3-yl)-7-methyl-4-phenyl-3,4-dihydro-2H-pyrano-[3,2-c]pyridin-5(6H)-one (4g’). Reflux 140 h, White solid; yield 57%; Mp 254.8–255.6 °C; IR: 3,418, 3,132, 3,058, 2,932, 1,616, 1,579, 1,455, 1,390, 1,363, 1,321, 1,260, 1,181, 1,155, 1,113, 1,039, 971, 872, 821, 765, 701, 663, and 598 cm-1; 1H-NMR (DMSO-d6) δ: 1.66 (d, 1H, J = 13.8 Hz, H8), 2.02 (s, 3H, 6-CH3), 2.09 (s, 3H, 6’-CH3), 3.10 (dt, 1H, J = 13.8, 4.8 Hz, H8’), 4.01 (d, 1H, J = 4.8 Hz, H7), 5.12 (dd, 1H, J = 12.3, 1.8 Hz, H9), 5.62 (s, 1H, H5), 5.65 (s, 1H, H5’), 7.08 (d, 2H, J = 7.2 Hz, H2” and H6”), 7.16 (t, 1H, J = 7.2 Hz, H4”), 7.26 (t, 2H, J = 7.2 Hz, H3” and H5”), 10.44 (s, 1H, 4’-OH); 11.02 (s, 2H, NH-1 and NH-1’); 13C-NMR (DMSO-d6) δ: 19.0 (6-CH3), 19.1 (6’-CH3), 32.5 (C8), 35.4 (C7), 67.2 (C9), 98.5 (C5), 98.7 (C5’), 104.6 (C3), 105.5 (C3’), 126.4 (C4”), 128.6 (C2” and C6”), 128.7 (C3” and C5”), 143.8 (C1”), 146.4 (C6 and C6’), 164.0 (C2), 164.3 (C2’), 164.9 (C4), 166.4 (C4’); HRMS (ESI+) C21H20N2NaO4 (MNa+) required 387.1315, found 387.1301.
(±)-3,7-Dimethyl-2-phenyl-2H-pyrano[3,2-c]pyridin-5(6H)-one (4h'). Reflux 190 h, White solid; yield 49%; Mp 222.4–223.3 °C; IR: 3,446, 3,058, 2,963, 2,897, 2,746, 1,669, 1,647, 1,630, 1,601, 1,565, 1,485, 1,453, 1,394, 1,263, 1,251, 1,145, 1,035, 951, 907, 869, 803, 770, 763, 696, 663, 649, 627, and 539 cm-1; 1H-NMR (DMSO-d6) δ: 1.61 (s, 3H, 8-CH3), 2.04 (s, 3H, 6-CH3), 5.59 (s, 1H, H5), 5.77 (s, 1H, H7), 6.40 (s, 1H, H9), 7.33-7.37 (m, 5H, H2’, H3’, H4’, H5’ and H6’), 11.20 (s, 1H, NH-1); 13C-NMR (DMSO-d6) δ: 19.2 (6-CH3), 20.0 (8-CH3), 81.0 (C7), 98.0 (C5), 104.4 (C3), 114.5 (C9), 127.0 (C8), 128.1 (C2’ and C6’), 129.4 (C3’ and C5’), 129.5 (C4’), 139.5 (C1’), 145.6 (C6), 160.2 (C2), 161.3 (C4); HRMS (ESI+) C16H15NNaO2 (MNa+) required 276.0995, found 276.0995.

3.2.4. Single crystal structure determination

The crystal structures of 4f, 4g’ and 4h’ were determined from single crystal X-ray diffraction data collected at 150 K with an Oxford Cryosystems Cryostream N2 open-flow cooling device [27]. Data were collected using an Enraf-Nonius KappaCCD diffractometer (Mo-Kα radiation (λ = 0.71073 Å) and processed using the DENZO-SMN package [28], including inter-frame scaling (which was carried out using SCALEPACK within DENZO-SMN). The structures were solved using SIR92 [29]. Refinement was carried out using full-matrix least-squares within the CRYSTALS suite [30] on F2. A small amount of disorder was present in 4f (alkyl bridge) and 4g’ (disordered DMSO) which was modeled as detailed in the supplementary information (CIF). Refinement results are included in Table 3 and full crystallographic data have also been deposited with the Cambridge Crystallographic Data Centre, CCDC 755517 – 755519. Copies of these data can be obtained free of charge from The Cambridge Crystallographic Data Centre via www.ccdc.cam.ac.uk/data_request/cif.
Table
Table 3. Crystal data and structure refinements for compounds 4f, 4g’ and 4h’.
Table 3. Crystal data and structure refinements for compounds 4f, 4g’ and 4h’.
4f4g’4h’
Chemical FormulaC21H22N2O4C21H20N2O4·0.421(C2H6OS)C16H15NO2
Fw366.41396.98253.11
Crystal system / Space groupC2/cP21/nP 21/c
Crystal colourColourlessColourlessColourless
Crystal size (mm)0.22 x 0.16 x 0.120.20 x 0.18 x 0.180.60 x 0.06 x 0.04
a (Å)21.848020.936513.8876
b (Å)12.12579.853905.7745
c (Å)16.740521.416016.4267
α (°)90.00090.00090.000
β (°)121.6650113.2622104.791
γ (°)90.00090.00090.000
V (Å3)3774.714059.081273.67
Z884
Dcalc (g/cm3)1.2891.3001.321
T (K)150150150
μ( Mo Kα) (mm-1)0.090.130.09
Reflections collected (Rint)4264 (0.0289)9268 (0.025)2923 (0.030)
Data/Restraints/Parameters2471/0/2549247/60/5522130/0/173
R1 (I>2σ(I))0.0590.0840.050
R1 / wR2 (all data)0.1420.1230.079

3.3. Plant growth inhibition assays

In order to evaluate the growth regulatory potential of the synthesized bis(pyridyl)methanes (4a-h), two different bioassays were carried out.

3.3.1. Radicle elongation assay on filter paper

The solutions of bis(pyridyl)methanes 4a-h were prepared by dissolving the compounds in dimethylsufoxide (150 µL). After addition of the surfactant Tween 80 (72 µL), the resulting suspension was transferred to a volumetric flask and diluted with water to 50 mL, to obtain the final concentration 5 × 10-4 mol L-1. These suspensions were sonicated for 5 min, resulting in complete dissolution of the test compound. Then 4 mL aliquots were used to imbibe two sheets of filter paper (Whatman nº 1) placed in 100 × 15 mm glass Petri dishes. To each dish were added 20 seeds of Sorghum bicolor L. Moench (Geneze Company, Paracatu, Minas Gerais State, Brazil) or Cucumis sativus L. (purchased from a local market). The plates were incubated at 25 ºC under fluorescent light (8 × 40 W) for 72 h. Radicle length was then measured, and total germination recorded. Seeds were considered to have germinated if a radicle protruded at least 1 mm. Treatments were carried out in a completely randomized design with five replications. The data, expressed as percentage of radical growth inhibition with respect to untreated controls, were analyzed using Tukey’s test at 0.05 probability level.

3.3.2. Greenhouse trials

Plastic pots (0.13 L) were filled with acid-washed sand, which was saturated with the solution of the test compound (60 mL/450 g of sand, corresponding to 6.7 × 10-8 mol a.i./g substrate). Four seeds of Ipomoea grandifolia, C. sativus or S. bicolor were placed in each pot. Seedlings were grown in a greenhouse, and watered as required with tap water or, twice a week, with half-strength Hoagland solution, to maintain the humidity at 13.3% w/w. Twenty-one days after sowing, plants were harvested, and the roots and aerial parts were separated and weighted. Tissues were then dried at 60 °C until there was no further weight loss, and the corresponding dry mass was determined. The percentage of root and aerial part growth inhibition was calculated in relation to the mass of the respective control. Data were expressed and analyzed as previously described [31].

4. Conclusions

We have described the preparation of a variety of functionalized bis(pyridyl)methanes, starting from dehydroacetic acid. We have demonstrated that the condensation of 4-hydroxypyridone 3 with cinnamaldehydes results in the formation of pyran derivatives. In addition, we have described the effect of the pyridone derivatives prepared on the aerial parts and on the radicle growth of the dicotyledonous species Ipomoea grandifolia and Cucumis sativus and the monocotyledonous species Sorghum bicolor. In general the compounds showed some phytotoxic selectivity, being more active against the dicotyledonous species. It was observed that all tested compounds inhibited the development of the roots of C. sativus by more than 50% and three of them inhibit the development of the aerial parts of I. grandifolia by more than 50%. Although no concrete structure-activity relationship analysis could be derived due to the limited number of compounds prepared, the phytotoxicity reported for this class of bis(pyridyl)methane substances is an indication of the potential of these substances as lead structures for the development of new compounds with increased activity. Work is currently underway to achieve this goal.

Acknowledgements

We are grateful to the following Brazilian agencies: CNPq (Conselho Nacional de Desenvolvimento Científico e Tecnológico) for financial support and research fellowships (AJD, LCAB) and FAPEMIG (Fundação de Amparo à Pesquisa do Estado de Minas Gerais) for financial support and a student fellowship (VMMV). We also thank the Indian trusts: Narotam Sekhsaria Foundation and J. N. Tata Endowment for financial support (AHR).
  • Sample Availability: Samples of the compounds 4a, 4b, 4c, 4e, 4f, 4g’ and 4h’ are available from the authors.

References and Notes

  1. Öztürk, G.; Erol, D.D.; Uzbay, T.; Aytemir, M.D. Synthesis of 4(1H)-pyridinone derivatives and investigation of analgesic and antiinflammatory activities. Farmaco 2001, 56, 251–256. [Google Scholar] [CrossRef]
  2. Findlay, J.A.; Tam, W.H.J.; Krepinsky, J. The chemistry of some 6-methyl-4-hydroxy-2-pyridones. Can. J. Chem. 1978, 56, 613–616. [Google Scholar] [CrossRef]
  3. Abadi, A.; Al-Deeb, O.; Al-Afify, A.; El-Kashef, H. Synthesis of 4-alkyl (aryl)-6-aryl-3-cyano-2(1H)-pyridinones and their 2-imino isosteres as nonsteroidal cardiotonic agents. Farmaco 1999, 54, 195–201. [Google Scholar] [CrossRef]
  4. Storck, P.; Aubertin, A.; Grierson, D.S. Tosylation/mesylation of 4-hydroxy-3-nitro-2-pyridinones as an activation step in the construction of dihydropyrido[3,4-b]benzo[f][1,4]thiazepin-1-one based anti-HIV agents. Tetrahedron Lett. 2005, 46, 2919–2922. [Google Scholar] [CrossRef]
  5. Macdonald, G.E.; Puri, A.; Shillinget, D.G. Interactive effect of photoperiod and fluridone on growth, reproduction, and biochemistry of dioecious hydrilla (Hydrilla verticillata). Weed Sci. 2008, 56, 189–195. [Google Scholar] [CrossRef]
  6. Wakabayashi, K.; Böger, P. Phytotoxic sites of action for molecular design of modern herbicides (Part 2): Amino acid, lipid and cell wall biosynthesis, and other targets for future herbicides. Weed Biol. Manag. 2004, 4, 59–70. [Google Scholar] [CrossRef]
  7. Evidente, A.; Fiore, M.; Bruno, G.; Sparapano, L.; Motta, A. Chemical and biological characterisation of sapinopyridione, a phytotoxic 3,3,6-trisubstituted-2,4-pyridione produced by Sphaeropsis sapinea, a toxigenic pathogen of native and exotic conifers, and its derivatives. Phytochemistry 2006, 67, 1019–1028. [Google Scholar]
  8. Cocco, M.T.; Congiu, C.; Onnis, V. Synthesis and antitumour activity of 4-hydroxy-2-pyridone derivatives. Eur. J. Med. Chem. 2000, 35, 545–552. [Google Scholar] [CrossRef]
  9. Cocco, M.T.; Congiu, C.; Onnis, V. New bis(pyridyl)methane derivatives from 4-hydroxy-2-pyridones: synthesis and antitumoral activity. Eur. J. Med. Chem. 2003, 38, 37–47. [Google Scholar] [CrossRef]
  10. Torres, M.; Gil, S.; Parra, M. New synthetic methods to 2-Pyridone rings. Curr. Org. Chem. 2005, 9, 1757–1780. [Google Scholar] [CrossRef]
  11. Jauasinghe, L.; Abbas, H.K.; Jacob, M.R.; Herath, W.H.M.W.; Nanayakkara, N.P.D. N-Methyl-4-hydroxy-2-pyridinone Analogues from Fusarium oxysporum. J. Nat. Prod. 2006, 69, 439–442. [Google Scholar] [CrossRef]
  12. Devi, N.S.; Perumal, S. A facile four-component tandem protocol for the synthesis of novel 2,6-diaryl-2,3-dihydro-1H-pyridin-4-ones. Tetrahedron Lett. 2007, 48, 5627–5629. [Google Scholar] [CrossRef]
  13. Pei, L.X.; Huang, S.L.; Shen, Y.D.; An, L.Q.; Huang, Z.S.; Li, Y.M.; Gu, L.Q.; Bu, X.Z.; Chan, A.S.C. Synthesis of novel dihydrofuro[b]-pyridinone derivatives: Oxidation coupling of 3-hydroxy-4(1H)-pyridinone with β-dicarbonyl compounds. Tetrahedron Lett. 2005, 46, 5085–5088. [Google Scholar]
  14. Barbosa, L.C.A.; Demuner, A.J.; Maltha, C.R.A.; Teixeira, R.R.; Souza, K.A.P.; Bicalho, K.U. Phytogrowth activity of 3-(3-chlorobenzyl)-5-arylidenofuran-2(5H)-ones. Z. Naturforsch. Sect. B 2009, 64, 245–251. [Google Scholar]
  15. Barbosa, L.C.A.; Nogueira, L.B.; Maltha, C.R.A.; Teixeira, R.R.; Silva, A.A. Synthesis and phytogrowth properties of oxabicyclic analogues related to helminthosporin. Molecules 2009, 14, 160–173. [Google Scholar] [CrossRef]
  16. Barbosa, L.C.A.; Teixeira, R.R.; Forlani, G.; Veloso, D.P.; Carneiro, J.W.M. Synthesis of photosynthesis-inhibiting nostoclide analogues. J. Agric. Food Chem. 2008, 56, 2321–2328. [Google Scholar]
  17. Barbosa, L.C.A.; Rocha, M.E.; Teixeira, R.R.; Maltha, C.R.A.; Forlani, G. Synthesis of 3-(4-bromobenzyl)-5-(aryl methylene)-5H-furan-2-ones and their activity as inhibitors of the photosynthetic electron transport chain. J. Agric. Food Chem. 2007, 55, 8562–8569. [Google Scholar]
  18. Barbosa, L.C.A.; Costa, A.V.; Demuner, A.J.; Silva, A.A. Synthesis and herbicidal activity of 2α,4α-dimethyl-8-oxabiciclo[3.2.1]oct-6-en-3-one derivatives. J. Agric. Food Chem. 2000, 47, 4807–4814. [Google Scholar]
  19. Lima, L.S.; Barbosa, L.C.A.; Alvarenga, E.S.; Demuner, A.J.; Silva, A.A. Synthesis and phytotoxicity evaluation of substituted para-benzoquinones. Aust. J. Chem. 2003, 36, 625–630. [Google Scholar]
  20. Alvarenga, E.S.; Barbosa, L.C.A.; Saliba, W.A.; Arantes, F.F.P.; Demuner, A.J.; SILVA, A.A. Síntese e avaliação da atividade fitotóxica de derivados da α-santonina. Quím. Nova 2009, 32, 401–406. [Google Scholar] [CrossRef]
  21. Barbosa, L.C.A.; Ferreira, M.L.; Demuner, A.J.; Silva, A.A.; Pereira, R.C. Preparation and phytotoxicity of sorgoleone analogues. Quím. Nova 2001, 24, 751–755. [Google Scholar] [CrossRef]
  22. Costa, A.V.; Barbosa, L.C.A.; Demuner, A.J.; Silva, A.A. Synthesis and Herbicidal activity of 2α,4α-dimethyl-8-oxabiciclo[3.2.1]oct-6-en-3-one derivatives. J. Agric. Food Chem. 1999, 47, 4807–4814. [Google Scholar] [CrossRef]
  23. Barbosa, L.C.A.; Chaves, F.C.; Demuner, A.J.; Silva, A.A. Synthesis of new helminthosporal analogues with plant-growth regulatory properties via oxyallyl cation. Z. Naturforsch. Sect. B 2006, 61, 1287–1291. [Google Scholar]
  24. Barbosa, L.C.A.; Demuner, A.J.; Maltha, C.R.A.; Silva, P.S. Síntese e avaliação da atividade fitotóxica de novos análogos oxigenados do ácido helmintospórico. Quím. Nova 2003, 26, 655–660. [Google Scholar] [CrossRef]
  25. Findlay, J.A.; Krepinsky, J.; Shum, F.Y.; Tam, W.H.J. Reactions of 4-hydroxy-6-methyl-2-pyridone with aldehydes. Can. J. Chem. 1976, 54, 270–274. [Google Scholar] [CrossRef]
  26. Lee, Y.R.; Kweon, H.; Koh, W.S.; Min, K.R.; Kim, Y.; Lee, S.H. One-Pot Preparation of pyranoquinolinones by ytterbium(III) trifluoromethanesulfonate-catalyzed reactions: Efficient synthesis of flindersine, N-methylflindersine, and zanthosimuline natural products. Synthesis 2001, 12, 1851–1855. [Google Scholar]
  27. Cosier, J.; Glazer, A.M. Anitrogen-gas-stream cryostat for general X-ray diffraction studies. J. Appl. Cryst. 1986, 19, 105–107. [Google Scholar] [CrossRef]
  28. Otwinowski, Z.; Minor, W. Methods in Enzymology; Carter, C.W., Jr., Sweet, R.M., Eds.; Academic Press: New York, NY, USA, 1997; Volume 276, p. 307. [Google Scholar]
  29. Altomare, A.; Cascarano, G.; Giacovazzo, G.; Guagliardi, A.; Burla, M.C.; Polidori, G.; Camalli, M. SIRPOW.92—A program for automatic solution of crystal structures by direct methods optimized for powder data. J. Appl. Cryst. 1994, 27, 435–436. [Google Scholar]
  30. Betteridge, P.W.; Carruthers, J.R.; Cooper, G.I.; Prout, C.K.; Watkin, D.J. CRYSTALS version 12: Software for guided crystal structure analysis. J. Appl. Cryst. 2003, 36, 1487–1487. [Google Scholar] [CrossRef]
  31. Dias, L.C.; Demuner, A.J.; Valente, V.M.M.; Barbosa, L.C.A.; Martins, F.T.; Dorigueto, A.C.; Ellena, J. Preparation of achiral and chiral E-enaminopyran-2,4-diones and their phytotoxic activity. J. Agric. Food Chem. 2009, 57, 1399–1405. [Google Scholar] [CrossRef]

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MDPI and ACS Style

Jacinto Demuner, A.; Moreira Valente, V.M.; Almeida Barbosa, L.C.; Rathi, A.; Donohoe, T.J.; Thompson, A.L. Synthesis and Phytotoxic Activity of New Pyridones Derived from 4-Hydroxy-6-Methylpyridin-2(1H)-one. Molecules 2009, 14, 4973-4986. https://doi.org/10.3390/molecules14124973

AMA Style

Jacinto Demuner A, Moreira Valente VM, Almeida Barbosa LC, Rathi A, Donohoe TJ, Thompson AL. Synthesis and Phytotoxic Activity of New Pyridones Derived from 4-Hydroxy-6-Methylpyridin-2(1H)-one. Molecules. 2009; 14(12):4973-4986. https://doi.org/10.3390/molecules14124973

Chicago/Turabian Style

Jacinto Demuner, Antonio, Vania Maria Moreira Valente, Luiz Cláudio Almeida Barbosa, Akshat Rathi, Timothy J. Donohoe, and Amber L. Thompson. 2009. "Synthesis and Phytotoxic Activity of New Pyridones Derived from 4-Hydroxy-6-Methylpyridin-2(1H)-one" Molecules 14, no. 12: 4973-4986. https://doi.org/10.3390/molecules14124973

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