Next Article in Journal
Palladium-Catalyzed Heck Coupling Reaction of Aryl Bromides in Aqueous Media Using Tetrahydropyrimidinium Salts as Carbene Ligands
Previous Article in Journal
Essential Oil Composition of Stems and Fruits of Caralluma europaea N.E.Br. (Apocynaceae)
 
 
Font Type:
Arial Georgia Verdana
Font Size:
Aa Aa Aa
Line Spacing:
Column Width:
Background:
Article

New Neolignans and a Phenylpropanoid Glycoside from Twigs of Miliusa mollis

1
Department of Pharmacognosy, Faculty of Pharmaceutical Sciences, Chulalongkorn University, Bangkok 10330, Thailand
2
Nationaal Herbarium Nederland, Leiden University, The Netherlands
*
Author to whom correspondence should be addressed.
Molecules 2010, 15(2), 639-648; https://doi.org/10.3390/molecules15020639
Submission received: 8 December 2009 / Revised: 20 January 2010 / Accepted: 22 January 2010 / Published: 28 January 2010

Abstract

:
From the twigs of Miliusa mollis Pierre, three new compounds including (2S,3S)-2,3-dihydro-2-(4-methoxyphenyl)-3-methyl-5-[1(E)-propenyl]benzofuran, (7S,8S)- threo8′-4-methoxyneolignan and tyrosol-1-O-β-xylopyranosyl-(1→6)-O-β-gluco-pyranoside were isolated, along with seven known compounds. Their structures were elucidated through analysis of their spectroscopic data.

Graphical Abstract

1. Introduction

The genus Miliusa Lesch. ex A.DC. (Annonaceae) comprises 30–40 species, which occur from India and South China to North Australia [1]. So far, there have been only a few reports on the constituents of plants in this genus, describing the presence of aporphine alkaloids, terpenoids, flavonoids, phenylpropanoids, styrylpyrones, bis-styryls and homogentistic acid derivatives [2,3,4,5,6,7,8,9,10,11,12]. Miliusa mollis Pierre, is a shrub found in the northern and central regions of Thailand where it is locally known as Ching-chap [13]. Prior to this investigation, no studies had been done to examine the chemical components of this plant. The current paper describes the isolation and structural elucidation of three new compounds from the twigs of M. mollis.

2. Results and Discussion

In this study, we report the isolation of two new neolignans including (2S,3S)-2,3-dihydro-2-(4-methoxyphenyl)-3-methyl-5-[1(E)-propenyl] benzofuran (1) and (7S,8S)- threo8′-4-methoxyneolignan (3), and a new glycosidic phenylpropanoid, namely tyrosol-1-O-β-xylopyranosyl-(1→6)-O-β-glucopyranoside (10), together with seven known compounds: (2R,3R)-2,3-dihydro-2-(4-hydroxy-3-methoxyphenyl)-3-methyl-5-(E)-propenylbenzofuran (2) [14], conocarpan (4) [14,15,16], (−)-epicatechin (5) [17,18], liriodenine (6) [19,20], asimilobine (7) [21,22], (−)-norushinsunine (8) [23] and icariside D2 (9) [24] (Figure 1). The structures of these known compounds were identified by comparison of their spectral data with those reported in the literature.
Compound 1 was obtained as a colorless oil. The positive HRESITOFMS exhibited an [M+Na]+ ion at m/z 303.1280, suggesting the molecular formula C19H20O2. The UV spectrum showed two absorption maxima at 228 and 274 nm, and the IR spectrum exhibited absorption bands for conjugated unsaturation (1,515 and 1,486 cm−1), and ether (1,243 cm−1) functionalities. The 1H-NMR signals at δ 5.09 (1H, d, J = 9.0 Hz, H-2), 3.39 (1H, m, H-3) and 1.39 (3H, d, J = 6.6 Hz, Me-3) and the 13C-NMR resonances at δ 92.6, 45.2, and 17.8 are characteristic features of the trans-2-aryl-3-methyl-2,3-dihydrobenzofuran system [14]. This was supported by the NOESY interactions of Me-3 protons with H-2. In the structure of 1, a methoxy group [δH 3.81 (3H, s); δC 55.3] was present at C-4′, as indicated from the HMBC correlations from the protons at δ 3.81 to C-4′ (δ 158.3), and from H-2′(6′) (δ 7.35, 2H, d, J = 8.7 Hz) to C-2 (δ 92.6) and C-4′. In addition, a 2-propenyl moiety [δH 6.37 (1H, d, J = 15.8 Hz, H-8), 6.09 (1H, dq, J = 15.8, 6.3 Hz, H-9), 1.86 (3H, d, J = 6.3 Hz, Me-10); δC 130.8 (C-8), 122.9 (C-9), 18.3 (C-10)] was located at C-5 (δ 131.2), as evidenced by the 3J-coupling from C-5 to H-9 (δ 6.09). These spectral data appeared to be superimposable on those reported for synthetic (±)-trans-2,3-dihydro-2-(4-methoxyphenyl)-3-methyl-5-(1(E)-propenyl)benzofuran [25]. It is known that a trans-2-aryl-3-methyl-2,3-dihydrobenzofuran structure with 2R,3R configuration shows a positive Cotton effect at about 260 nm in the CD spectrum, whereas the reverse is true for the 2S,3S-isomer [14]. Since 1 showed a negative optical rotation ( [ α ] D 20 13.22 ) and its CD curve exhibited a negative Cotton effect at 264 nm, the structure of 1 was determined as (2S,3S)-2,3-dihydro-2-(4-methoxyphenyl)-3-methyl-5-[1(E)-propenyl]benzofuran (Figure 1). Figure 2 shows the CD curve of 1, in contrast with that of 4, which is in the 2R,3R series. It should be noted that although the antipodal isomer of 1 was earlier mentioned [26,27], its spectroscopic data were not provided.
Compound 3 gave an [M+Na]+ ion at m/z 321.1375 in the HRESITOFMS, indicating a molecular formula of C19H22O3. The UV spectrum showed absorption maxima at 227 and 275 nm, and the IR spectrum demonstrated absorption bands for hydroxyl (3,448 cm−1), conjugated unsaturation (1,509 cm−1), and ether (1,243 cm−1) functionalities. The 13C-NMR spectrum of 3 (Table 1) showed a nineteen-carbon structure with two p-disubstituted benzene rings. In support of this, two pairs of doublets appeared at δ 7.32 (2H, d, J = 8.6 Hz, H-2 and H-6) and 6.88 (2H, d, J = 8.6 Hz, H-3 and H-5), and at δ 7.09 (2H, d, J = 8.4 Hz, H-2′ and H- 6′) and 6.87 (2H, d, J = 8.4 Hz, H-3′ and H-5′) in the 1H-NMR spectrum. In the HMQC spectrum, two tertiary oxygenated carbon signals appearing at δ 77.7 (C-7) and 79.3 (C-8) showed direct coupling with protons at δ 4.62 (1H, d, J = 7.7 Hz, H-7) and 4.34 (1H, dq, J = 7.7, 6.2 Hz, H-8), respectively. These two methine protons constituted an ABX coupling system with the Me protons at δ 1.07 (3H, d, J = 6.2 Hz, Me-9) in the COSY spectrum.
Moreover, H-2 and H-6 exhibited 3-bond coupling with C-7, whereas H-8 showed HMBC connectivity to C-4′ through an ether linkage (Table 1). These spectral data of 3 were similar to those of previously reported 8-O-4′neolignans [28]. Compound 3 should have a methoxy group (δH 3.79, 3H, s; δC 55.3) at C-4 and an allyl moiety [(δH 3.32 (2H, br d, J = 6.6 Hz), 5.05 (2H, dd, J = 10.2, 16.8 Hz) and 5.93 (1H, m); δC 39.3, 115.5 and 137.7) at C-1′. The placement of the MeO group at C-4 was supported by the HMBC correlation from the MeO-4 protons (δ 3.79) to C-4 (159.6), which in turn showed 3J-coupling with H-2 and H-6. In accordance with this proposed structure, HMBC correlations were observed from C-1′ to H-3′(5′) and H-7′. It is known that for neolignans of this skeleton, the large coupling constant (J = 7.7 Hz) for H-7 and H-8, which was due to the intramolecular hydrogen bonding of the benzylic hydroxyl and the aryloxyl group, suggested a threo relative configuration [29,30]. On the basis of the negative and positive peaks at 276 and 233 nm, respectively in the CD spectrum (Figure 3), the absolute configurations at C-7 and C-8 of 3 were both assigned to be S [30]. Based on the above evidence, the structure of 3 was determined to be (7S,8S)-threo8′-4-methoxyneolignan.
Compound 10 was obtained as a colorless amorphous powder. It has a molecular formula of C19H28O11, as indicated by the [M+Na]+ ion peak at m/z 455.1619 in the HRESITOFMS. The compound showed UV absorptions at 223 and 273 nm, and IR bands at 3,366 (hydroxyl), 1,510 (conjugated unsaturation), and 1,071 and 1,043 (ether) cm−1. Compound 10 appeared to be a glycoside with tyrosol (4-hydroxyethylphenol) [31] as the aglycon, as suggested from the aromatic proton resonances at δ 7.10 (2H, d, J = 8.6 Hz, H-3 and H-5) and 6.95 (2H, d, J = 8.6 Hz, H-2 and H-6), and the aliphatic proton signals at δ 2.64 (2H, t, J = 6.5 Hz, H-7) and 3.54 (2H, t, J = 6.5 Hz, H-8) (Table 2). This was supported by the 13C-NMR signals at δ 155.7 (C-1), 132.7 (C-4), 129.7 (C-3 and C-5), and 116.2 (C-2 and C-6), 38.2 (C-7) and 62.4 (C-8) [31].
Apart from the tyrosol moiety, compound 10 possessed two sugar units, as evidenced by two anomeric protons at δ 4.73 (1H, d, J = 7.3 Hz, H-1′) and 4.17 (1H, d, J = 7.6 Hz, H-1″), which were correlated to the carbons at δ 100.7 (C-1′) and 103.8 (C-1″), respectively, in the HMQC spectrum. The inner sugar was β-glucopyranose [δH 4.73 (1H, d, J = 7.3 Hz, H-1′), 3.22 (2H, m, H-2′ and H-3′), 3.14 (1H, t, J = 8.8 Hz, H-4′), 3.48 (1H, dd, J = 8.8, 6.6 Hz, H-5′), 3.55 (1H, dd, 10.9, 6.6 Hz, H-6′a) and 3.93 (1H, dd, 10.9, 8.8 Hz, H-6′b); δC δ 100.7 (C-1′), 73.2 (C-2′), 76.5 (C-3′), 69.6 (C-4′), 75.8 (C-5′) and 68.2 (C-6′)] [32], and its connection to the aglycon through an arylether bond was demonstrated by the HMBC correlation from H-1′ to C-1 (δ 155.7) and the NOESY interaction of H-1′ with H-2(6). The other sugar unit was β-xylopyranose [δH 4.17 (1H, d, J = 7.6 Hz, H-1″), 2.96 (1H, dd, J = 8.7, 7.6 Hz, H-2″), 3.06 (1H, t, J = 8.7 Hz, H-3″), 3.22 (1H, m, H-4″), 3.65 (1H, dd, J = 11.3, 5.3 Hz, H-5″b), 2.94 (1H, t, J = 11.3 Hz, H-5″a); δC δ 103.8 (C-1″), 73.4 (C-2″), 76.5 (C-3″), 69.6 (C-4″), 65.6 (C-5″)], with its anomeric carbon linked to C-6′ of the glucose moiety through an ether bridge [30,33]. This linkage was further confirmed by the HMBC correlations between C-1″ and H2-6′, and between C-6′ and H-1″. Thus, the structure of 10 was determined to be tyrosol-1-O-β-xylopyranosyl-(1→6)-O-β-glucopyranoside.
It should be noted that although neolignans are frequently identified from the Annonaceae, they were not previously found in the genus Miliusa, and this is the first time that neolignans were isolated from a plant of this genus.

3. Experimental

3.1. General

Optical rotations were measured on a Perkin-Elmer 341 polarimeter, and the CD spectra were recorded on a JASCO J-715 spectropolarimeter. UV spectra were obtained on a Shimadzu UV-160A UV/vis spectrometer and IR spectra on a Perkin-Elmer FT-IR 1760X spectrophotometer. Mass spectra were recorded on a Micromass LCT mass spectrometer or a Thermo-Finnigan Polaris Q mass spectrometer. NMR spectra were obtained with a Bruker Avance DPX-300 FT-NMR spectrometer (300 MHz) or a JEOL JMN-A 500 NMR spectrometer (500 MHz). Vacuum-liquid column chromatography (VLC), column chromatography (CC) and medium pressure liquid chromatography (MPLC) were performed with silica gel 60 (Merck, Kieselgel 60, 70-230 mesh), silica gel 60 (Merck, Kieselgel 60, 230-400 mesh), Diaion HP20SS (Mitsubishi Chemical Co.) and Sephadex LH-20 (25–100 μm, Pharmacia Fine Chemical Co. Ltd.). Preparative TLC was carried out with silica gel plate (Merck, Kieselgel 60 F254).

3.2. Plant Material

The twigs of Miliusa mollis Pierre were collected in Bangkok, Thailand by one of us (T.C.) and identified by R. W. J. M. van der Ham, as previously described [1].

3.3. Extraction and Isolation

The dried and powdered plant material (380 g) was extracted with MeOH (3 × 3L) to give 24 g of an extract, which was then subjected to VLC on silica gel using solvent mixtures of increasing polarity (n-hexane, CH2Cl2, EtOAc and MeOH) to give eight fractions (A-H).
Fraction D (98 mg) was further separated by CC on silica gel (15.4 g) with gradient elution (n-hexane-CH2Cl2) to give seven fractions (D1–D7). Fraction D4 (33 mg) was purified on Sephadex LH-20 (CH2Cl2-MeOH 1:1) to give 1 (22 mg).
Fraction E (321 mg) was separated by CC on silica gel (21.62 g) with n-hexane-CH2Cl2 gradient elution to give ten fractions (E1-E10). Fraction E6 (184 mg) was separated on Sephadex LH-20 (CH2Cl2-MeOH 1:1) to give 2 (100 mg).
Fraction F (1.9 g) was separated by MPLC (silica gel, n-hexane-CH2Cl2 gradient elution) to give eleven fractions (F1–F11). Fraction F7 (171.9 mg) was separated with Sephadex-LH-20/CH2Cl2-MeOH (1:1) to give six fractions (F7-1 to F7-6). Fraction F7-4 (7 mg) was purified by preparative TLC (silica gel, n-hexane-EtOAc-acetone 90:8:2) to yield 2 mg of 3. Fraction F8 (379 mg) was separated with Sephadex LH-20/CH2Cl2-MeOH (1:1) to give 4 (339.0 mg).
Fraction G (1.6 g) was separated by MPLC (silica gel, n-hexane-EtOAc gradient elution) to give twelve fractions (G1–G12). Fraction G9 (433.4 mg) was separated on Sephadex LH-20 (acetone) to give 5 (163 mg).
Fraction H (16.5 g) was fractionated on Diaion HP20SS, eluted with H2O-MeOH (100:0–0:100) to give seven fractions (H1-H7). Fraction H7 (405.9 mg) was separated on silica gel (21.0 g) with EtOAc-MeOH-H2O gradient elution to give fourteen fractions (H7-1 to H7-14). Fraction H7-2 (25 mg) was further purified with Sephadex-LH-20/CH2Cl2-MeOH (1:1) to give 6 (2 mg). Fraction H7-6 (52 mg) was separated on Sephadex LH-20, eluted with CH2Cl2-MeOH (1:1) to give four fractions (H7-6-1 to H7-6-4). Fraction H7-6-4 (10 mg) was further purified by CC (silica gel, CH2Cl2-acetone 1:4) to give 7 (2 mg). Fraction H7-7 (38 mg) was separated with Sephadex LH-20/CH2Cl2-MeOH (1:1) to give four fractions (H7-7-1 to H7-7-4). Fraction H7-7-4 (15 mg) was further purified by CC on silica gel, eluted with CH2Cl2-acetone (1:4) to give 2 mg of 7 and 5 mg of 8. Fraction H4 (282 mg) was separated by CC on silica gel (19.6 g) with EtOAC-MeOH-H2O gradient elution to give twelve fractions (H4-1 to H4-12). Fraction H4-3 (16 mg) was separated on Sephaddex LH-20(MeOH) to give three fractions (H4-3-1 to H4-3-3). Fraction H4-3-1 (14 mg) was further purified by preparative TLC (silica gel) with EtOAc-MeOH-H2O (92:6:2) to yield 10 mg of 9. Fraction H4-6 (39 mg) was separated on Sephadex LH-20 (MeOH) to give six fractions (H4-6-1 to H4-6-6). Fraction H4-6-4 (26 mg) was further purified by CC (silica gel, EtOAc-MeOH-H2O 80:12:8) to give 10 (3 mg).
(2S,3S)-2,3-Dihydro-2-(4-methoxyphenyl)-3-methyl-5-[1(E)-propenyl]benzofuran (1): colorless oil; [ α ] D 20 13.22 (c 0.42, MeOH); CD (MeOH, c 0.001): [θ]300 −401, [θ]264 −2,296, [θ]226 +1,791; EI-MS m/z 281 [M+1]+, 280 [M]+, 265, 251, 157, 148, 135, 131, 115, 103, 91; HRESITOFMS m/z 303.1280 [M+Na]+ (calcd. for C19H20O2Na, 303.1361); UV λmax (MeOH) nm (log ε) 228 (3.76), 274 (3.26); IR νmax (film): 1515, 1486, 1243 cm-1; 1H-NMR (300 MHz, CDCl3): δ 7.35 (2H, d, J = 8.7 Hz, H-2′, H-6′), 7.14 (1H, br s, H-4), 7.12 (1H, d, J = 8.1 Hz, H-6), 6.91 (2H, d, J = 8.7 Hz, H-3′, H-5′), 6.76 (1H, d, J = 8.1 Hz, H-7), 6.37 (1H, d, J = 15.8 Hz, H-8), 6.09 (1H, dq, J = 15.8, 6.3 Hz, H-9), 5.09 (1H, d, J = 9.0 Hz, H-2), 3.81 (3H, s, MeO), 3.39 (1H, m, H-3), 1.86 (3H, d, J = 6.3 Hz, Me-10), 1.39 (3H, d, J = 6.6 Hz, Me-3); 13C-NMR (75 MHz, CDCl3): δ 159.6 (C-7a), 158.3 (C-4′), 132.7 (C-3a), 132.4 (C-1′), 131.2 (C-5), 130.8 (C-8), 127.6 (C-2′, C-6′), 126.3 (C-6), 122.9 (C-9), 120.7 (C-4), 114.0 (C-3′,C-5′), 109.2 (C-7), 92.6 (C-2), 45.2 (C-3), 55.3 (MeO), 18.3 (C-10), 17.8 (Me-3).
(7S,8S)-threo-Δ8-4-methoxyneolignan (3): colorless oil; [ α ] D 20 + 10.0 (c 0.05, MeOH); CD (MeOH, c 0.002): [θ]276 −1,932, [θ]233 +2,392; EI-MS m/z 298 [M]+, 281, 162, 161, 137, 133, 121, 115, 105, 91, 77; HRESITOFMS m/z 321.1375 [M+Na]+ (calcd. for C19H22O3Na, 321.1468); UV λmax (MeOH) nm (log ε) 227 (4.18), 275 (3.48); IR νmax (film): 3448 (br), 1509, 1243 cm-1; 1H-NMR (300 MHz, CDCl3) and 13C-NMR (75 MHz, CDCl3): see Table 1.
Tyrosol-1-O-β-xylopyranosyl-(1→6)-O-β-glucopyranoside (10): colorless amorphous powder; [ α ] D 20 48.75 (c 0.08, MeOH); EI-MS m/z 414, 207, 167, 149, 138, 107, 77; HRESITOFMS m/z 455.1619 [M+Na]+ (calcd. for C19H28O11Na, 455.1529); UV λmax (MeOH) nm (log ε) 223 (3.51), 273 (2.77); IR νmax (film): 3366 (br), 1510, 1071, 1043 cm-1; 1H-NMR (500 MHz, DMSO-d6) and 13C-NMR (125 MHz, DMSO-d6): see Table 2.

4. Conclusion

Three new compounds including (2S,3S)-2,3-dihydro-2-(4-methoxyphenyl)-3-methyl-5-[1(E)-propenyl]benzofuran, (7S,8S)- threo8′-4-methoxyneolignan and tyrosol-1-O-β-xylopyranosyl-(1→6)-O-β-glucopyranoside were isolated from the twigs of Miliusa mollis Pierre. The presence of neolignans in the genus Miliusa was reported for the first time in this study.

Acknowledgements

K.S. acknowledges the financial support from The Thailand Research Fund through the Royal Golden Jubilee Ph.D. Program (PHD/0261/2549). She is also a recipient of Chulalongkorn University Graduate Scholarship in Commemoration of HM King Bhumibol Adulyadej’s 72nd Anniversary.

References

  1. Chaowasku, T.; Mols, J.; van der Ham, R.W.J.M. Pollen morphology of Miliusa and relatives (Annonaceae). Grana 2008, 47, 175–184. [Google Scholar] [CrossRef]
  2. Harrigan, G.G.; Gunatilaka, A.A.L.; Kingston, D.G.I.; Chan, G.W.; Johnson, R.K. Isolation of bioactive and other oxoaporphine alkaloids from two annonaceous plants, Xylopia aethiopica and Miliusa cf. banacea. J. Nat. Prod. 1994, 57, 68–73. [Google Scholar] [CrossRef] [PubMed]
  3. Jumana, S.; Hasan, C.M.; Rashid, M.A. Alakaloids from the stem bark of Miliusa velutina. Biochem. Syst. Ecol. 2000, 28, 483–485. [Google Scholar] [CrossRef]
  4. Wu, R.; Ye, Q.; Chen, N.Y.; Zhang, G.L. A new norditerpene from Miliusa balansae Finet et Gagnep. Chin Chem Lett 2001, 12, 247–248. [Google Scholar]
  5. Kamperdick, C.; Hong Van, N.; Van Sung, T. Constituents from Miliusa balansae (Annonaceae). Phytochemistry 2002, 61, 991–994. [Google Scholar] [CrossRef]
  6. Chen, B.; Feng, C.; Li, B.G.; Zhang, G.L. Two new alkaloids from Miliusa cuneata. Nat. Prod. Res. 2003, 17, 397–402. [Google Scholar] [CrossRef] [PubMed]
  7. Huong, D.T.; Kamperdick, C.; Van Sung, T. Homogentisic acid derivatives from Miliusa balansae. J. Nat. Prod. 2004, 67, 445–447. [Google Scholar] [CrossRef] [PubMed]
  8. Brophy, J.J.; Goldsack, R.J.; Forster, P.I. The leaf oils of the Australian species of Miliusa (Annonaceae). J. Essent. Oil Res. 2004, 16, 253–255. [Google Scholar] [CrossRef]
  9. Huong, D.T.; Luong, D.V.; Thao, T.T.P.; Sung, T.V. A new flavone and cytotoxic activity of flavonoid constituents isolated from Miliusa balansae (Annonaceae). Pharmazie 2005, 60, 627–629. [Google Scholar] [CrossRef] [PubMed]
  10. Zhang, H.J.; Ma, C.Y.; Van Hung, N.; Cuong, N.M.; Tan, G.T.; Santarsiero, B.D.; Mesecar, A.D.; Soejarto, D.D.; Pezzuto, J.M.; Fong, H.H.S. Miliusanes, a class of cytotoxic agents from Miliusa sinensis. J. Med. Chem. 2006, 49, 693–708. [Google Scholar] [CrossRef] [PubMed]
  11. Lei, Y.; Wu, L.J.; Shi, H.M.; Tu, P.F. Three new glycosides from the stems of Miliusa balansae. Helv. Chim. Acta 2008, 91, 495–500. [Google Scholar] [CrossRef]
  12. Huong, D.T.; Van, N.T.H.; Kamperdick, C.; Anh, N.T.H.; Sung, T.V. Two new bis-styryl compounds from Miliusa balansae. Z. Naturforsch. B. 2008, 63, 335–338. [Google Scholar] [CrossRef]
  13. Smitinand, T. Thai Plant Names, revised Ed.; Prachachon Co. Ltd.: Bangkok, Thailand, 2001; p. 359. [Google Scholar]
  14. Achenbach, H.; Grob, J.; Domínguez, X.A.; Cano, G.; Star, J.V.; Brussolo, L.D.C.; Munoz, G.; Salgado, F.; LÓpez, L. Lignans, neolignans and norneolignans from Krameria cystisoides. Phytochemistry 1987, 26, 1159–1166. [Google Scholar] [CrossRef]
  15. Chauret, D.C.; Bernard, C.B.; Arnason, J.T.; Durst, T.; Krishnamurty, H.G.; SanchezVindas, P.; Moreno, N.; San Roman, L.; Poveda, L. Insecticidal neolignans from Piper decurrens. J. Nat. Prod. 1996, 59, 152–155. [Google Scholar] [CrossRef] [PubMed]
  16. Achenbach, H.; Utz, W.; Lozano, B.; Touché, E.M.G.; Moreno, S. Lignans and neolignans from Krameria parvifolia. Phytochemistry 1996, 43, 1093–1095. [Google Scholar] [CrossRef]
  17. Shahat, A.A. Procyanidins from Adansonia digitata. Pharm. Biol. 2006, 44, 445–450. [Google Scholar] [CrossRef]
  18. Foo, L.Y.; Newman, R.; Waghorn, G.; McNabb, W.C.; Ulyatt, M.J. Proanthocyanidins from Lotus corniculatus. Phytochemistry 1996, 41, 617–624. [Google Scholar] [CrossRef]
  19. Pang, S.Q.; Wang, G.Q.; Huang, B.K.; Zhang, Q.Y.; Qin, L.P. Isoquinoline alkaloids from Broussonetia papyrifera fruits. Chem. Nat. Compd. 2007, 43, 100–102. [Google Scholar] [CrossRef]
  20. Zhang, Z.Z.; ElSohly, H.N.; Jacob, M.R.; Pasco, D.S.; Walker, L.A.; Clark, A.M. New sesquiterpenoids from the root of Guatteria multivenia. J. Nat. Prod. 2002, 65, 856–859. [Google Scholar] [CrossRef] [PubMed]
  21. Fischer, D.C.H.; Gonçalves, M.I.; Oliveira, F.; Alvarenga, M.A. Constituents from Siparuna apiosyce. Fitoterapia 1999, 70, 322–323. [Google Scholar] [CrossRef]
  22. Zanin, S.M.W.; Lordello, A.L.L. Alcalóides aporfinóides do gênero Ocotea (Lauraceae). Quim. Nova 2007, 30, 92–98. [Google Scholar] [CrossRef]
  23. Lo, W.L.; Wu, Y.C.; Hsieh, T.J.; Kuo, S.H.; Lin, H.C.; Chen, C.Y. Chemical constituents from the stems of Michelia compressa. Chin. Pharm. J. 2004, 56, 69–75. [Google Scholar]
  24. Miyase, T.; Ueno, A.; Takizawa, N.; Kobayashi, H.; Oguchi, H. Ionone and lignan glycosides from Epimedium diphyllum. Phytochemistry 1989, 28, 3483–3485. [Google Scholar] [CrossRef]
  25. Snider, B.B.; Han, L.N.; Xie, C.Y. Synthesis of 2,3-dihydrobenzofurans by Mn(OAc)3-based oxidative cycloaddition of 2-cyclohexenones with alkenes. Synthesis of (±)-conocarpan. J. Org. Chem. 1997, 62, 6978–6984. [Google Scholar] [CrossRef]
  26. Achenbach, H.; Utz, W.; Usubillaga, A.; Rodriguez, H.A. Lignans from Krameria ixina. Phytochemistry 1991, 30, 3753–3757. [Google Scholar] [CrossRef]
  27. Achenbach, H.; Utz, W.; Sánchez, H.; Touché, E.M.G.; Verde, J.; Dominguez, X.A. Neolignans, nor-neolignans and other compounds from roots of Krameria grayi. Phytochemistry 1995, 39, 413–415. [Google Scholar] [CrossRef]
  28. Braga, A.C.H.; Zacchino, S.; Badano, H.; Sierra, M.G.; Rúveda, E.A. 13C NMR spectral and conformational analysis of 8-O-4′ neolignans. Phytochemistry 1984, 23, 2025–2028. [Google Scholar] [CrossRef]
  29. Morais, S.K.R.; Teixeira, A.F.; Torres, Z.E.D.S.; Nunomura, S.M.; Yamashiro-Kanashiro, E.H.; Lindoso, J.A.L.; Yoshida, M. Biological activities of lignoids from amazon Myristicaceae species: Virola michelii, V. mollissima, V. pavonis and Iryanthera juruensis. J. Brazil. Chem. Soc. 2009, 20, 1110–1118. [Google Scholar] [CrossRef]
  30. Huo, C.H.; Liang, H.; Zhao, Y.Y.; Wang, B.; Zhang, Q.Y. Neolignan glycosides from Symplocos caudata. Phytochemistry 2008, 69, 788–795. [Google Scholar] [CrossRef] [PubMed]
  31. Sommart, U.; Rukachaisirikul, V.; Sukpondma, Y.; Phongpaichit, S.; Towatana, N.H.; Graidist, P.; Hajiwangoh, Z.; Sakayaroj, J.A. Cyclohexenone derivative from Diaporthaceous fungus PSU-H2. Arch. Pharm. Res. 2009, 32, 1227–1231. [Google Scholar] [CrossRef] [PubMed]
  32. Agrawal, P.K. NMR spectroscopy in the structural elucidation of oligosaccharides and glycosides. Phytochemistry 1992, 31, 3307–3330. [Google Scholar] [CrossRef]
  33. Schroeder, C.; Lutterbach, R.; Stöckigt, J. Preparative biosynthesis of natural glucosides and fluorogenic substrates for β-glucosidases followed by in vivo 13C NMR with high density plant cell cultures. Tetrahedron 1996, 52, 925–934. [Google Scholar] [CrossRef]
Sample Availability: Samples of the compounds are available from the authors.
Figure 1. Compounds isolated from Miliusa mollis.
Figure 1. Compounds isolated from Miliusa mollis.
Molecules 15 00639 g001
Figure 2. CD data of compounds 1 and 4.
Figure 2. CD data of compounds 1 and 4.
Molecules 15 00639 g002
Figure 3. CD of data compound 3.
Figure 3. CD of data compound 3.
Molecules 15 00639 g003
Table 1. 1H- (300 MHz) and 13C-NMR (75 MHz) data of 3 (CDCl3, δ in ppm and J in Hz) and HMBC correlations.
Table 1. 1H- (300 MHz) and 13C-NMR (75 MHz) data of 3 (CDCl3, δ in ppm and J in Hz) and HMBC correlations.
Position1H13CHMBC (correlation with 1H)
1-132.0 (s)3, 5 and 7
27.32 (1H, d, 8.6)128.5 (d)6 and 7
36.88 (1H, d, 8.6)113.9 (d)5
4-159.6 (s)2, 6 and MeO
56.88 (1H, d, 8.6)113.9 (d)3
67.32 (1H, d, 8.6)128.5 (d)2 and 7
74.62 (1H, d, 7.7)77.7 (d)8 and 9
84.34 (1H, dq, 7.7, 6.2)79.3 (d)9
91.07 (3H, d, 6.2)15.7 (q)
1′-133.1 (s)3′, 5′ and 7′
2′7.09 (1H, d, 8.4)129.7 (d)6′ and 7′
3′6.87 (1H,d, 8.4)116.4 (d)
4′-156.1 (s)8, 2′ and 6′
5′6.87 (1H,d, 8.4)116.4 (d)
6′7.09 (1H, d, 8.4)129.7 (d)2′ and 7′
7′3.32 (2H, br d, 6.6)39.3 (t)
8′5.93 (1H, m)137.7 (d)7′
9′5.05 (2H, dd, 10.2, 16.8)115.5 (t)7′
MeO-43.79 (3H, s)55.3 (q)-
Table 2. 1H- (500 MHz) and 13C-NMR (125 MHz) data of 10 (DMSO-d6, δ in ppm and J in Hz) and HMBC correlations.
Table 2. 1H- (500 MHz) and 13C-NMR (125 MHz) data of 10 (DMSO-d6, δ in ppm and J in Hz) and HMBC correlations.
Position1H13CHMBC (correlation with 1H)
1-155.7 (s)2, 3, 5, 6 and 1′
26.95 (1H, d, 8.6)116.2 (d)3 and 6
37.10 (1H, d, 8.6)129.7 (d)2, 5 and 7
4-132.7 (s)2, 6, 7 and 8
57.10 (1H, d, 8.6)129.7 (d)3, 6 and 7
66.95 (1H, d, 8.6)116.2 (d)2 and 5
72.64 (2H, t, 6.5)38.2 (t)3, 5 and 8
83.54 (2H, t, 6.5)62.4 (t)7
1′4.73 (1H, d, 7.3)100.7 (d)5′
2′3.22 (1H, m)73.2 (d)3′
3′3.22 (1H, m)76.5 (d)1′
4′3.14 (1H, t, 8.8)69.6 (d)2′,3′, 5′ and 6′b
5′3.48 (1H, dd, 8.8, 6.6)75.8 (d)1′ and 6′a
6′a3.55 (1H, dd, 10.9, 6.6)68.2 (t)-
6′b3.93 (1H, dd, 10.9, 8.8)-5′ and 1″
4.17 (1H, d, 7.6)103.8 (d)5″a and 5″b, 6′a, 6′b
2.96 (1H, dd, 8.7, 7.6)73.4 (d)1″ and 3″
3.06 (1H, t, 8.7)76.5 (d)2″, 5″a and 5″b
3.22 (1H, m)69.6 (d)2″, 3″, 5″a and 5″b
a2.94 (1H, t, 11.3)65.6 (t)-
b3.65 (1H, dd, 11.3, 5.3)-1″

Share and Cite

MDPI and ACS Style

Sawasdee, K.; Chaowasku, T.; Likhitwitayawuid, K. New Neolignans and a Phenylpropanoid Glycoside from Twigs of Miliusa mollis. Molecules 2010, 15, 639-648. https://doi.org/10.3390/molecules15020639

AMA Style

Sawasdee K, Chaowasku T, Likhitwitayawuid K. New Neolignans and a Phenylpropanoid Glycoside from Twigs of Miliusa mollis. Molecules. 2010; 15(2):639-648. https://doi.org/10.3390/molecules15020639

Chicago/Turabian Style

Sawasdee, Kanokporn, Tanawat Chaowasku, and Kittisak Likhitwitayawuid. 2010. "New Neolignans and a Phenylpropanoid Glycoside from Twigs of Miliusa mollis" Molecules 15, no. 2: 639-648. https://doi.org/10.3390/molecules15020639

Article Metrics

Back to TopTop