Design, Synthesis and Biological Evaluation of Hydroxamic Acid Derivatives as Potential High Density Lipoprotein (HDL) Receptor CLA-1 Up-Regulating Agents

Abstract

Trichostatin A (TSA) and suberoylanilide hydroxamic acid (SAHA) were reported in our recent publication as novel human high density lipoprotein (HDL) receptor CD36 and Lysosomal integral membrane protein-II Analogous-1 (CLA-1) up-regulators. As part of a broader effort to more fully explore the structure-activity relationships (SAR) of CLA-1 up-regulators, we synthesized a series of hydroxamic acid derivatives and evaluated their CLA-1 up-regulating activities in HepG2 cells. Some compounds exhibited over 10-fold up-regulation of CLA-1 expression in HepG2 cells at 10 μg/mL concentration. The compound 1g showed the best potency, with a lower EC₅₀ than TSA (EC₅₀ = 0.32 μM versus 1.2 μM). These compounds provide early new CLA-1 up-regulators with potential for treating atherosclerosis.

1. Introduction

Atherosclerosis is one of the leading causes of mortality in industrialized and developing nations. It is a progressive disease that is characterized by the accumulation of lipid-rich plaques within the walls of arteries [1]. Long-term clinical studies have shown that plasma concentrations of high density lipoprotein (HDL) cholesterol (HDL-C) are inversely proportional to the risk for atherosclerotic cardiovascular disease. One of the major atheroprotective actions of HDL particles involves the transport of excess cholesterol from peripheral tissues to the liver for excretion, a process known as reverse cholesterol transport (RCT) [2]. HDL-mediated RCT represents a major target for the development of innovative antiatherogenic strategies to reduce the risk of atherosclerotic cardiovascular disease.

Scavenger receptor class B type I (SR-BI) is the first molecularly well-defined HDL receptor in mice [3], and its human homologue is CD36 and Lysosomal integral membrane protein-II Analogous-1 (CLA-1) [4]. SR-BI/CLA-1 plays an important role in RCT by mediating selective uptake of cholesteryl ester from peripheral tissues to the liver. The discovery of up-regulators of CLA-1 expression may benefit the further study of the mechanism of action of CLA-1 in human atherosclerotic cardiovascular diseases and might have pharmacologic applications [5].

To obtain active compounds that can increase CLA-1 transcriptional level in liver cells, we developed a cell-based reporter assay applicable for high-throughput screening (HTS) [6]. Using this assay trichostatin A (TSA) was found to prominently up-regulate CLA-1 transcriptional activity [7]. Suberoylanilide hydroxamic acid (SAHA), an analogue of TSA, was also found to up-regulate CLA-1 transcriptional activity [7]. TSA and SAHA have some common pharmacophore characteristics, which can be segmented into four parts: (i) a terminal aromatic unit (TAU); (ii) a connecting unit (CU); (iii) a linker domain; and (iv) the hydroxamic acid group (HAG) (Figure 1) [8,9]. Small molecular structure changes have been found to have significant impact on up-regulating activity. When the hydroxamic acid group was replaced by a carboxyl and an acylamide, the two corresponding analogues of TSA showed a 10-fold and a 250-fold decrease in maximal up-regulating fold compared with TSA, respectively [10]. To better understand the SAR of hydroxamic acid compounds, to lay out a foundation for potential up-regulators of CLA-1 expression, and to further explore the mechanism of CLA-1/SR-BI promoter up-regulation we embarked on the design and synthesis of analogues library of TSA and SAHA.

Figure 1. Structures of TSA, SAHA and designed compounds.

Figure 1. Structures of TSA, SAHA and designed compounds.

Based on TSA and SAHA, primarily the hydroxamic acid group was retained and the lead change program focused on the linker and terminal aromatic units. In TSA the chain length between the CU and the hydroxamic acid group is five atoms and it is six atoms for SAHA. Firstly we adjusted the length of the linker to 4–5 carbon atoms based on SAHA to obtain compounds 1a-i. TSA has two methyl groups on the linker part, so piperazine ring was introduced in different locations in the linker in 2a-e, mainly based on SAHA, to investigate space tolerance. Furthermore we introduced a benzylamino group to the linker part to synthesize compounds 3a-o to investigate its effect on up-regulating activity. Compounds 12a-d with a carboxyl group replacing the hydroxamic acid group of 3a-d were also investigated.

1.1. Chemistry

Scheme 1 shows the synthetic routes used to prepare the three series of hydroxamic acid compounds 1a-i, 2a-e and 3a-o. Reactions of adipic acid monoethyl ester or pimelic acid monoethyl ester 4 with relevant amines using 1-ethyl-3-(3-dimethyllaminopropyl)carbodiimide hydrochloride (EDC·HCl) as a coupling agent gave acylamides 5 [11], which were then reacted with hydroxylamine in the presence of NaOH, yielding the desired targets 1a-i [12]. Bromoacetic acid or 3-bromopropionic acid 6 were reacted with relevant amines using EDC·HCl to obtain acylamides 7, which were in turn reacted with 1-BOC-piperazine in the presence of K₂CO₃ to give 8. The BOC groups of compounds 8 were deprotected with 3 N HCl to afford 9 [13], which were reacted with ethyl bromoacetate or ethyl 3-bromopropionate in the presence of K₂CO₃ to give 10 [14]. Compounds 10 were finally converted into 2a-e in the same way as described for 1a-i. Acylamides 7 with methyl 4-(aminomethyl)benzoate hydrochloride in the presence of KHCO₃ furnished 11, which were then converted to 3a-o according to the synthetic method used for 1a-i.

Scheme 1. The synthesis route of objective compounds.

Scheme 1. The synthesis route of objective compounds.

2. Results and Discussion

As exploratory screening of the synthesized compounds, we first evaluated the up-regulating of CLA-1 expression activities in HepG2 cells at 10 μg/mL concentration. Table 1, Table 2, Table 3 summarize the activity data and inhibition rates of HDAC at 500 nM for the synthesized compounds as well as the known up-regulators TSA and SAHA as positive controls.

Table 1. Up-regulating activities of CLA-1 expression in HepG2 cells and inhibition rate of HDAC of compounds 1a-i.

Cpd. a	Ar	n	Up-regulating fold b	Inhibition rate
			(SD c)	of HDAC (%) d
1a		1	5.4 (1.4)	8.8
1b		1	9.4 (1.3)	18.8
1c		1	5.9 (1.8)	18.8
1d		1	3.6 (0.3)	9.6
1e		2	13.8 (2.1)	34.9
1f		2	9.2 (2.3)	23.2
1g		2	13.3 (3.4)	92.1
1h		2	10.4 (1.4)	51.2
1i		2	15.7 (2.5)	39.9
SAHA			15.5 (0.5)	72.9
TSA			35.7 (1.8)	95.0

^a All compounds tested were >95% pure by HPLC; ^b All compounds were tested at 10 μg/mL except that SAHA was at 2.5 μM and TSA at 3.0 μM; ^c Standard deviation; ^d All compounds were tested at 500 nM.

Table 1. Up-regulating activities of CLA-1 expression in HepG2 cells and inhibition rate of HDAC of compounds 1a-i.

Cpd. a	Ar	n	Up-regulating fold b	Inhibition rate
			(SD c)	of HDAC (%) d
1a		1	5.4 (1.4)	8.8
1b		1	9.4 (1.3)	18.8
1c		1	5.9 (1.8)	18.8
1d		1	3.6 (0.3)	9.6
1e		2	13.8 (2.1)	34.9
1f		2	9.2 (2.3)	23.2
1g		2	13.3 (3.4)	92.1
1h		2	10.4 (1.4)	51.2
1i		2	15.7 (2.5)	39.9
SAHA			15.5 (0.5)	72.9
TSA			35.7 (1.8)	95.0

^a All compounds tested were >95% pure by HPLC; ^b All compounds were tested at 10 μg/mL except that SAHA was at 2.5 μM and TSA at 3.0 μM; ^c Standard deviation; ^d All compounds were tested at 500 nM.

Table 2. Up-regulating activities of CLA-1 expression in HepG2 cells and inhibition rate of HDAC of compounds 2a-e.

Cpd.	R	n	m	Up-regulating fold	Inhibition rate
				(SD)	of HDAC (%)
2a	H	2	1	1.2 (0.1)	5.4
2b	2,4-(OCH3)	2	1	1.1 (0.08)	7.2
2c	H	1	1	1.1 (0.1)	5.6
2d	4-N(CH3)2	1	1	1.1 (0.2)	5.9
2e	H	1	2	1.1 (0.1)	8.6

Table 2. Up-regulating activities of CLA-1 expression in HepG2 cells and inhibition rate of HDAC of compounds 2a-e.

Cpd.	R	n	m	Up-regulating fold	Inhibition rate
				(SD)	of HDAC (%)
2a	H	2	1	1.2 (0.1)	5.4
2b	2,4-(OCH3)	2	1	1.1 (0.08)	7.2
2c	H	1	1	1.1 (0.1)	5.6
2d	4-N(CH3)2	1	1	1.1 (0.2)	5.9
2e	H	1	2	1.1 (0.1)	8.6

Table 3. Up-regulating activities of CLA-1 expression in HepG2 cells and inhibition rate of HDAC of compounds 3a-o, 12a-d.

Cpd.	Ar’	n	R’	Up-regulating fold	Inhibition rate
				(SD)	of HDAC (%)
3a		1	NHOH	10.1 (0.8)	23.3
3b		1	NHOH	6.6 (0.02)	23.7
3c		1	NHOH	0.89 (0.05)	34.2
3d		1	NHOH	12.4 (1.1)	28.0
3e		1	NHOH	10.5 (0.6)	28.3
3f		1	NHOH	2.9 (0.4)	21.3
3g		1	NHOH	8.1 (0.3)	9.8
3h		1	NHOH	4.6 (0.1)	29.8
3i		1	NHOH	5.0 (0.4)	16.5
3j		1	NHOH	1.9 (0.4)	10.9
3k		2	NHOH	8.2 (1.6)	35.0
3l		2	NHOH	13.4 (4.8)	45.7
3m		2	NHOH	5.2 (1.1)	25.5
3n		2	NHOH	1.4(0.3)	13.0
3o		2	NHOH	7.2 (1.8)	27.7
12a		1	COOH	1.5 (0.3)	-
12b		1	COOH	1.1 (0.02)	-
12c		1	COOH	1.1 (0.1)	-
12d		1	COOH	1.2 (0.1)	-

Table 3. Up-regulating activities of CLA-1 expression in HepG2 cells and inhibition rate of HDAC of compounds 3a-o, 12a-d.

Cpd.	Ar’	n	R’	Up-regulating fold	Inhibition rate
				(SD)	of HDAC (%)
3a		1	NHOH	10.1 (0.8)	23.3
3b		1	NHOH	6.6 (0.02)	23.7
3c		1	NHOH	0.89 (0.05)	34.2
3d		1	NHOH	12.4 (1.1)	28.0
3e		1	NHOH	10.5 (0.6)	28.3
3f		1	NHOH	2.9 (0.4)	21.3
3g		1	NHOH	8.1 (0.3)	9.8
3h		1	NHOH	4.6 (0.1)	29.8
3i		1	NHOH	5.0 (0.4)	16.5
3j		1	NHOH	1.9 (0.4)	10.9
3k		2	NHOH	8.2 (1.6)	35.0
3l		2	NHOH	13.4 (4.8)	45.7
3m		2	NHOH	5.2 (1.1)	25.5
3n		2	NHOH	1.4(0.3)	13.0
3o		2	NHOH	7.2 (1.8)	27.7
12a		1	COOH	1.5 (0.3)	-
12b		1	COOH	1.1 (0.02)	-
12c		1	COOH	1.1 (0.1)	-
12d		1	COOH	1.2 (0.1)	-

Compounds 1a-i with a linker chain of four or five carbon atoms showed promising up-regulating activity. In general, compounds 1e-i with five carbon atoms chain showed more powerful activity than 1a-d with 9.2~15.7 fold up-regulation. From the structural perspective, the carbon chain length has great influence on up-regulating activity; the longer chain with five carbon atoms is more active than the shorter one. Compounds 1a-e, 1g, 1i were selected for further studies to obtain their activity EC₅₀ values. Similarly compounds with five carbon atoms chain showed better activities than those with four carbon atoms chain. Figure 2 shows that compound 1g with a 3-chloro substituent on the phenyl ring exhibited the best up-regulating activity in terms of EC₅₀, suggesting that the substitution of the phenyl ring seemed to have significant impact on the up-regulating activity. Compound 1g has an EC₅₀ value of 0.32 μM, which is much lower than those of the reference compounds TSA (EC₅₀ = 1.2 μM) and SAHA (EC₅₀ = 2.1 μM), and more importantly, it showed much better up-regulating activity than both reference compounds at low concentrations (at and below 1 μM).

Figure 2. The dose-response curves of compound 1g and SAHA.

Figure 2. The dose-response curves of compound 1g and SAHA.

In contrast, 2a-e incorporating a piperazine group on the linker part exhibited no up-regulating activity, suggesting that the position 1 to 2 carbon atoms away from hydroxamic acid can’t tolerate the bulky piperazine ring group.

To determine if the CLA-1 expression level was increased by 1g due to the up-regulation of transcriptional activity, flow cytometry was performed to investigate the abundance of CLA-1 protein levels in HepG2 cells with and without 1g treatment. The result showed that with treatment of 0.3 μM 1g, the protein level of CLA-1 was increased by 224.6% (Figure 3A), which was higher than 0.3 μM SAHA (63.5%). To test whether 1g enhanced the selective uptake of lipids from HDL by increasing the expression of CLA-1, fluorescence-labeled DiI-HDL uptake after 12 h incubation with HepG2 cells was measured in the presence or absence of 1g. 0.3 μM 1g promoted the uptake of DiI-HDL into the HepG2 cells by 62.5% which was higher than 0.3 μM SAHA (24.1%) (Figure 3B).

Figure 3. Effect of compound 1g on CLA-1 expression and DiI-HDL uptake.

Figure 3. Effect of compound 1g on CLA-1 expression and DiI-HDL uptake.

TSA was reported as a potent specific reversible inhibitor of mammalian histone deacetylase (HDAC), leading to hyperacetylation of chromatin-associated histones and thereby open promoter loci to permit interaction with transcription factors to promote gene expression [15]. SAHA was the first HDAC inhibitor approved for treating cutaneous T cell lymphoma by FDA in 2006. It was reported that there was a 30-fold increase in HDAC inhibitory activity progressing from aceto- to benzoyl hydroxamic acid, with IC₅₀ values of 625 and 25 μM, respectively (Figure 4) [16,17]. In addition, MS-275 is under clinical trials and K-182 is powerful as HDAC inhibitors [18]. They all possess a benzylamino group on the linker part. We also tried to introduce a benzylamino moiety into the linker part to synthesize compounds 3a-o. Compounds 3a-o showed moderate activity. When n = 1, phenyl 3a, p-chloro phenyl 3d, p-methoxyphenyl 3e showed activity, with over 10-fold up-regulation at 10 μg/mL concentration, while 3b and 3c with chloro group substitution in the ortho and meta positions show a great decline in activity. Likewise, 3,4-dimethoxyphenyl compound 3f also displayed reduced activity. Benzyl or phenylethyl compounds 3h-3j were inferior to those with phenyl groups. When n = 2, p-chlorophenyl derivative 3l was more potent than the o-chlorophenyl one 3k. The 3,4-dimethoxy-phenyl compound 3n showed a fourfold decrease in activity compared with the similar p-methoxy-phenyl analog 3m.

Figure 4. Structures of HDAC inhibitors.

Figure 4. Structures of HDAC inhibitors.

For 12a-d, the most notable observation is the lack of activity of all the carboxylic acid derivatives tested; conversely, nearly all hydroxamic acid analogs showed promising up-regulating activity. Consistent with our previous results [10], these results indicated that the hydroxamic acid group is indispensable. For this series of compounds, substitution at the para-position of the phenyl ring is preferred for good activity.

At present, there are only some clues that several known HDAC inhibitors such as TSA, SAHA and sodium butyrate are active on CLA-1 up-regulating activity [7]. However, TSA up-regulated CLA-1 transcription with EC₅₀ = 1.2 μM, which is much higher than its HDAC inhibition IC₅₀ values (usually at nanomolar level). As the relationship between HDAC inhibition and CLA-1 transcriptional upregulation is quite intriguing, we further detected the HDAC inhibitory activity of our compounds using a HDAC Fluorimetric Assay kit (Enzo Life Science). At 10 nM, none of all 29 compounds showed HDAC inhibition (<5% inhibition) except compound 1g (~25% inhibition), whereas TSA exhibited ~60% inhibition at the same concentration (data not shown). At 500 nM, 1a-i and 3a-o showed different HDAC inhibition rates ranging from 8.8% to 92.1% and compounds 2a-e showed little inhibition (<8.6%) (Table 1, Table 2, Table 3). Compound 1g showed the highest HDAC inhibition rate of 92.1% whereas TSA and SAHA exhibited 72.9% and 95.0% inhibition, respectively (Table 1, Table 2, Table 3). The HDAC inhibition activity of the compounds synthesized in the present study correlated positively to their CLA-1 up-regulation activity on the whole, suggesting CLA-1 up-regulation may be dependent on the HDAC inhibition. However, the activity of some compounds towards HDAC inhibition did not correlate with the degree of up-regulation of CLA-1 promoter. This suggests that some of the hydroxamic acid derivatives may affect CLA-1 transcription through mechanisms other than HDAC inhibition in HepG2 cells. It will be interesting to determine the detailed relationship between HDAC inhibition and the induction of CLA-1 transcription in future studies.

3. Experimental

3.1. General

All reagents and solvents were reagent grade or were purified by standard methods before use. Melting points were determined in open capillaries on a RT-1 melting point apparatus (Tianjin Fenxi Yiqichang, Tianjin, China) and are uncorrected. Column chromatography was carried out on flash MCI GEL 20Y. ¹H-NMR spectra analysis was performed on a Varian Inova 400 MHz spectrometer (Varian, Palo Alto, CA, USA), using DMSO-d₆ as solvent and Me₄Si as the internal standard. Chemical shifts (δ values) and coupling constants (J values) are given in ppm and Hz, respectively. ESI high-resolution mass spectra (HRMS) analysis was recorded on an Autospec Ultima-TOF mass spectrometer (Micromass UK Ltd., Manchester, UK). All the HRMS data were within ±5 ppm of calculated values.

3.2. General Procedure for the Amide Bond Formation (Scheme 1, step a)

A solution of aniline (1.86 g, 20.0 mmol) in CH₂Cl₂ (10.0 mL) was added dropwise to a cooled (−5 °C) solution of 3-bromopropionic acid (3.06 g, 20.0 mmol) and EDC·HCl (3.84 g, 20.0 mmol) in CH₂Cl₂ (20.0 mL). The mixture was allowed to warm at room temperature and stirred overnight. The progress of the reaction was monitored by Thin-Layer Chromatography (TLC; eluent: dichloromethane/ethyl acetate 5:1). After disappearance of the starting material, the mixture was washed with 1 mol/L HCl (30 mL), 5% solution of Na₂HCO₃ (30 mL) and brine. The organic layer was dried over anhydrous sodium sulfate and concentrated to give the crude material (about 60% yield) which was used for the next reaction.

3.3. General Procedure for the Amine Bond Formation (Scheme 1, step c)

The corresponding bromide (10.0 mmol), methyl 4-(aminomethyl)benzoate hydrochloride (2.02 g, 10.0 mmol) and potassium bicarbonate (1.50 g, 15.0 mmol) were dissolved in CH₃CN (100 mL). The solution was stirred at reflux for 3–4 h, and then concentrated under reduced pressure. To the residue was added water and ethyl acetate. HCl solution (1 mol/L) was added to organic layer and precipitate was filtered. The sediment was dried (about 50% yield) and used for the next reaction.

3.4. General Procedure for Boc Deblocking (Scheme 1, step d)

Concentrated HCl (15 mL) was dropped into a solution of the corresponding BOC protected compound (5.82 mmol) in ethyl alcohol (50 mL), and the mixture was stirred for 16 h at room temperature. Saturated solution of NaHCO₃ was added to pH > 8. The solution was concentrated under reduced pressure and the residue was extracted with ethyl acetate. The organic layer was concentrated under reduced pressure and provided the desired product as a yellow oil (about 80% yield).

3.5. General Procedure for the Synthesis of Hydroxamic Acids from Methyl Esters (Scheme 1, step b)

The corresponding methyl or ethyl esters 5, 10, 11 (5.0 mmol), hydroxylamine hydrochloride (280 mg, 4 mmol) were dissolved in MeOH (50 mL), and then 8 mol/L NaOH solution (10 mL) was added. The solution was stirred at ambient temperature for 1~2 h. To the solution was added 1 mol/L HCl (50 mL) at 0 °C until pH = 7~8, then the solution was concentrated under reduced pressure. The residue was purified by flash chromatography MCI GEL 20Y (water/methanol) to get the target compounds 1a-i, 2a-e, 3a-o.

Adipoyl anilide hydroxamic acid (1a). A yellowish solid, 670 mg, yield: 59.2%; m.p. 180–181.5 °C; ¹H-NMR: 1.52 (4H, m, 2CH₂), 1.96 (2H, t, J = 6.8, CH₂), 2.28 (2H, t, J = 6.8, CH₂), 7.00 (1H, t, J = 8.0, Ar-H), 7.27 (2H, t, J = 8.0, Ar-H), 7.55 (2H, d, J = 8.0, Ar-H), 8.65 (1H, s, CONHOH), 9.84 (1H, s, NHCO), 10.34 (1H, s, CONHOH); MS (ESI) m/z: 237 (M+H)⁺; HRMS (ESI) m/z: (M+H)⁺ calcd. for C₁₂H₁₇N₂O₃, 237.1234; found, 237.1227.

Adipoyl (3-chlorophenyl)amide hydroxamic acid (1b). A khaki solid, 712 mg, yield: 67.4%; m.p. 169–171 °C; ¹H-NMR: 1.52 (4H, m, 2CH₂), 1.96 (2H, t, J = 6.8, CH₂), 2.29 (2H, t, J = 6.8, CH₂), 7.07 (1H, d, J = 8.0, Ar-H), 7.30 (1H, t, J = 8.0, Ar-H), 7.42 (1H, d, J = 8.0, Ar-H), 7.81 (1H, s, Ae-H), 8.65 (1H, s, CONHOH), 10.05 (1H, s, NHCO), 10.34 (1H, s, CONHOH). MS (ESI) m/z: 271 (M+H)⁺; HRMS (ESI) m/z: (M+H)⁺ calcd. for C₁₂H₁₆ClN₂O₃, 271.0849; found, 271.0839.

Adipoyl (4-chlorophenyl)amide hydroxamic acid (1c). A white solid, 543 mg, yield: 71.0%; m.p. 176–177 °C; ¹H-NMR: 1.52 (4H, m, 2CH₂), 1.96 (2H, t, J = 6.8, CH₂), 2.28 (2H, t, J = 6.8, CH₂), 7.32 (2H, d, J = 8.8, Ar-H), 7.60 (2H, d, J = 8.8, Ar-H), 8.65 (1H, s, CONHOH), 9.99 (1H, s, NHCO), 10.33 (1H, s, CONHOH); MS (ESI) m/z: 271 (M+H)⁺; HRMS (ESI) m/z: (M+H)⁺ calcd. for C₁₂H₁₆ClN₂O₃, 271.0849; found, 271.0836.

Adipoyl (4-methoxybenzyl)amide hydroxamic acid (1d). A khaki solid, 398 mg, yield: 35.9%; m.p. 140–142 °C; ¹H-NMR: 1.46 (4H, m, 2CH₂), 1.93 (2H, t, J = 6.8, CH₂), 2.09 (2H, t, J = 6.8, CH₂), 3.71 (3H, s, OCH₃), 4.16 (2H, d, J = 6.0, CH₂NH), 6.86 (2H, d, J = 8.4, Ar-H), 7.14 (2H, d, J = 8.4, Ar-H), 8.21 (1H, t, J = 6.0, NHCO), 8.65 (1H, s, CONHOH), 10.34 (1H, s, CONHOH); MS (ESI) m/z: 281 (M+H)⁺; HRMS (ESI) m/z: (M+H)⁺ calcd. for C₁₄H₂₁N₂O₄, 281.1496; found, 281.1487.

Pimeloyl anilide hydroxamic acid (1e). A khaki solid, 666 mg, yield: 75.8%; m.p. 148–150 °C; ¹H-NMR: 1.26 (2H, m, CH₂), 1.53 (4H, m, 2CH₂), 1.93 (2H, t, J = 7.2, CH₂), 2.27 (2H, t, J = 7.2, CH₂), 7.00 (1H, t, J = 8.0, Ar-H), 7.26 (2H, t, J = 8.0, Ar-H), 7.57 (2H, d, J = 8.0, Ar-H), 8.64 (1H, s, CONHOH), 9.83 (1H, s, NHCO), 10.31 (1H, s, CONHOH); MS (ESI) m/z: 251 (M+H)⁺; HRMS (ESI) m/z: (M+H)⁺ calcd. for C₁₃H₁₈N₂O₃, 251.1390; found, 251.1385.

Pimeloyl (2-chlorophenyl)amide hydroxamic acid (1f). A white solid, 359 mg, yield: 43.8%; m.p. 62–64 °C; ¹H-NMR: 1.28 (2H, m, CH₂), 1.47 (4H, m, 2CH₂), 1.92 (2H, m, CH₂), 2.33 (2H, m, CH₂), 7.17 (1H, m, Ar-H), 7.30 (1H, t, J = 7.2, Ar-H), 7.47 (1H, m, Ar-H), 7.65 (1H, d, J = 7.2, Ar-H), 8.64 (1H, s, CONHOH), 9.43 (1H, s, NHCO), 10.32 (1H, s, CONHOH); MS (ESI) m/z: 285 (M+H)⁺; HRMS (ESI) m/z: (M+H)⁺ calcd. for C₁₃H₁₈ClN₂O₃, 285.1001; found, 285.0995.

Pimeloyl (3-chlorophenyl)amide hydroxamic acid (1g). A white solid, 723 mg, yield: 77.2%; m.p. 138–140 °C; ¹H-NMR: 1.25 (2H, m, CH₂), 1.53 (4H, m, 2CH₂), 1.93 (2H, t, J = 7.2, CH₂), 2.29 (2H, t, J = 7.2, CH₂), 7.06 (1H, dd, J = 8.0, 2.0, Ar-H), 7.30 (1H, t, J = 8.0, Ar-H), 7.42 (1H, d, J = 8.0, Ar-H), 7.81 (1H, t, J = 2.0, Ar-H), 8.64 (1H, s, CONHOH), 10.04 (1H, s, NHCO), 10.31 (1H, s, CONHOH); MS (ESI) m/z: 285 (M+H)⁺; HRMS (ESI) m/z: (M+H)⁺ calcd. for C₁₃H₁₈ClN₂O₃, 285.1001; found, 285.0994.

Pimeloyl (4-chlorophenyl)amide hydroxamic acid (1h). A white solid, 219 mg, yield: 53.3%; m.p. 161–162 °C; ¹H-NMR: 1.25 (2H, m, CH₂), 1.53 (4H, m, 2CH₂), 1.93 (2H, t, J = 7.6, CH₂), 2.28 (2H, t, J = 7.6, CH₂), 7.32 (2H, d, J = 8.8, Ar-H), 7.60 (2H, d, J = 8.8, Ar-H), 8.63 (1H, s, CONHOH), 9.98 (1H, s, NHCO), 10.31 (1H, s, CONHOH); MS (ESI) m/z: 285 (M+H)⁺; HRMS (ESI) m/z: (M+H)⁺ calcd. for C₁₃H₁₈ClN₂O₃, 285.1001; found, 285.0995.

Pimeloyl (4-methoxybenzyl)amide hydroxamic acid (1i). A white solid, 441 mg, yield: 41.7%; m.p. 133–135 °C; ¹H-NMR: 1.20 (2H, m, CH₂), 1.47 (4H, m, 2CH₂), 1.91 (2H, t, J = 7.2, CH₂), 2.08 (2H, t, J = 7.2, CH₂), 3.71 (3H, s, OCH₃), 4.16 (2H, d, J = 5.6, CH₂NH), 6.86 (2H, d, J = 8.8, Ar-H), 7.14 (2H, d, J = 8.8, Ar-H), 8.22 (1H, t, J = 5.6, NHCO); MS (ESI) m/z: 295 (M+H)⁺; HRMS (ESI) m/z: (M+H)⁺ calcd. for C₁₅H₂₃N₂O₄, 295.1657; found, 295.1645.

N-Hydroxy-2-(4-phenylcarbamoylethyl-piperazin-1-yl)-acetamide (2a). A white solid, 35 mg, yield: 62.4%; m.p. 75–77 °C. ¹H-NMR: 2.42–2.61 (12H, m, 6CH₂), 3.28 (2H, s, CH₂), 7.01 (1H, t, J = 7.6, Ar-H), 7.26 (2H, m, Ar-H), 7.55 (2H, d, J = 8.0, Ar-H), 10.06 (1H, m, NHCO); MS (ESI) m/z: 307 (M+H)⁺; HRMS (ESI) m/z: (M+H)⁺ calcd. for C₁₅H₂₃N₄O₃, 307.1765; found, 307.1754.

N-Hydroxy-2-{4-[(3,4-dimethoxyphenylcarbamoyl)-ethyl]piperazin-1-yl}acetamide (2b). A yellowish solid, 21 mg, yield: 54.9%; m.p. 69–71 °C; ¹H-NMR: 2.39–2.58 (12H, m, 6CH₂), 3.28 (2H, s, CH₂), 3.69 (3H, s, OCH₃), 3.70 (3H, s, OCH₃), 6.85 (1H, d, J = 8.8, Ar-H), 7.04 (1H, m, Ar-H), 7.27 (1H, d, J = 2.0, Ar-H), 9.94 (1H, m, NHCO); MS (ESI) m/z: 367 (M+H)⁺; HRMS (ESI) m/z: (M+H)⁺ calcd. for C₁₇H₂₇N₄O₅, 367.1976; found, 367.1966.

N-Hydroxy-2-(4-phenylcarbamoylmethylpiperazin-1-yl)acetamide (2c). A yellowish solid, 13 mg, yield: 79.8%; m.p. 56–58 °C; ¹H-NMR: 2.41–2.73 (8H, m, 4CH₂), 3.09 (2H, s, CH₂), 3.10 (2H, s, CH₂), 7.04 (1H, t, J = 7.6, Ar-H), 7.26 (2H, m, Ar-H), 7.55 (2H, d, J = 8.0, Ar-H), 9.64 (1H, s, NHCO); MS (ESI) m/z: 293 (M+H)⁺; HRMS (ESI) m/z: (M+H)⁺ calcd. for C₁₄H₂₁N₄O₃, 293.1608; found, 293.1598.

N-Hydroxy-2-{4-[(4-dimethylaminophenylcarbamoyl)methyl]piperazin-1-yl}acetamide (2d). A white solid, 33 mg, yield: 44.4%; m.p. 217–219 °C; ¹H-NMR: 2.48 (4H, m, 2CH₂), 2.83 (10H, m, 2CH₂, 2CH₃), 3.03 (2H, s, CH₂), 3.30 (3H, s, OCH₃), 6.67 (2H, d, J = 9.2, Ar-H), 7.39 (2H, d, J = 9.2, Ar-H), 9.33 (1H, s, NHCO); MS (ESI) m/z: 336 (M+H)⁺; HRMS (ESI) m/z: (M+H)⁺ calcd. for C₁₆H₂₆N₅O₃, 336.2030; found, 336.2018.

N-Hydroxy-3-(4-phenylcarbamoylmethylpiperazin-1-yl)propionamide (2e). A white solid, 29 mg, yield: 65.1%; m.p. 55–57 °C; ¹H-NMR: 2.23 (2H, m, CH₂), 2.51–2.64 (10H, m, 5CH₂), 3.18 (2H, s, CH₂), 7.06 (1H, t, J = 7.6, Ar-H), 7.31 (2H, m, Ar-H), 7.55 (2H, d, J = 7.6, Ar-H), 8.77 (1H, s, CONHOH), 9.75 (1H, s, NHCO), 10.46 (1H, s, CONHOH); MS (ESI) m/z: 307 (M+H)⁺; HRMS (ESI) m/z: (M+H)⁺ calcd. for C₁₅H₂₃N₄O₃, 307.1765; found, 307.1753.

N-Hydroxy-4-{[(phenylcarbamoylmethyl)amino]methyl}benzamide (3a). A white solid, 67 mg, yield: 47.7%; m.p. 124–126 °C. ¹H-NMR: 3.26 (2H, s, CH₂CO), 3.77 (2H, s, NHCH₂), 7.01–7.71 (9H, m, Ar-H), 9.80 (1H, s, CONH); MS (ESI) m/z: 300 (M+H)⁺; HRMS (ESI) m/z: (M+H)⁺ calcd. for C₁₆H₁₈N₃O₃, 334.0953; found, 334.0952.

N-Hydroxy-4-{[(2-chlorophenylcarbamoylmethyl)amino]methyl}benzamide (3b). A white solid, 72 mg, yield: 57.0%; m.p. 160–162 °C; ¹H-NMR: 3.31 (2H, s, CH₂CO), 3.82 (2H, s, NHCH₂), 7.12 (1H, m, Ar-H), 7.33 (1H, m, Ar-H), 7.45 (2H, d, J = 8.0, Ar-H), 7.50 (1H, m, Ar-H), 7.71 (2H, d, J = 8.0, Ar-H), 8.24 (1H, m, Ar-H), 8.98 (1H, s, CONHOH), 10.05 (1H, s, NHCO), 11.15 (1H, s, CONHOH); MS (ESI) m/z: 334 (M+H)⁺; HRMS (ESI) m/z: (M+H)⁺ calcd. for C₁₆H₁₇ClN₃O₃, 334.0953; found, 334.0952.

N-Hydroxy-4-{[(3-chlorophenylcarbamoylmethyl)amino]methyl}benzamide (3c). A yellowish solid, 45 mg, yield: 66.4%; m.p. 127–129 °C; ¹H-NMR: 3.27 (2H, s, CH₂CO), 3.76 (2H, s, NHCH₂), 7.09 (1H, d, J = 8.0, Ar-H), 7.32 (1H, t, J = 8.0, Ar-H), 7.41 (2H, d, J = 8.0, Ar-H), 7.48 (1H, d, J = 8.0, Ar-H), 7.70 (2H, d, J = 8.0, Ar-H), 7.84 (1H, s, Ar-H), 9.99 (1H, s, NHCO); MS (ESI) m/z: 334 (M+H)⁺; HRMS (ESI) m/z: (M+H)⁺ calcd. for C₁₆H₁₇ClN₃O₃, 334.0953; found, 334.0957.

N-Hydroxy-4-{[(4-chlorophenylcarbamoylmethyl)amino]methyl}benzamide (3d). A white solid, 67 mg, yield: 31.9%; m.p. 162–163 °C; ¹H-NMR: 3.26 (2H, s, CH₂CO), 3.77 (2H, s, NHCH₂), 7.34 (2H, d, J = 8.4, Ar-H), 7.42 (2H, d, J = 8.0, Ar-H), 7.64 (2H, d, J = 8.4, Ar-H), 7.70 (2H, d, J = 8.0, Ar-H), 8.97 (1H, s, CONHOH), 9.94 (1H, s, NHCO), 11.14 (1H, s, CONHOH); MS (ESI) m/z: 334 (M+H)⁺; HRMS (ESI) m/z: (M+H)⁺ calcd. for C₁₆H₁₇ClN₃O₃, 334.0953; found, 334.0953.

N-Hydroxy-4-{[(4-methoxyphenylcarbamoylmethyl)amino]methyl}benzamide (3e). A white solid, 38 mg, yield: 68.9%; m.p. 156–157 °C; ¹H-NMR: 2.79 (1H, brs, NH), 3.22 (2H, s, CH₂CO), 3.71 (3H, s, OCH₃), 3.76 (2H, s, NHCH₂), 6.86 (2H, d, J = 8.8, Ar-H), 7.42 (2H, d, J = 8.0, Ar-H), 7.50 (2H, d, J = 8.8, Ar-H), 7.70 (2H, d, J = 8.0, Ar-H), 8.96 (1H, s, CONHOH), 9.66 (1H, s, NHCO), 11.14 (1H, s, CONHOH); MS (ESI) m/z: 330 (M+H)⁺; HRMS (ESI) m/z: (M+H)⁺ calcd. for C₁₇H₂₀N₃O₄, 330.1448; found, 330.1448.

N-Hydroxy-4-{[(3,4-dimethoxyphenylcarbamoylmethyl)amino]methyl}benzamide (3f). A white solid, 35 mg, yield: 57.3%; m.p. 175–177 °C; ¹H-NMR: 3.16 (2H, s, CH₂CO), 3.70 (3H, s, OCH₃), 3.71 (3H, s, OCH₃), 3.78 (2H, s, NHCH₂), 6.86 (1H, d, J = 8.8, Ar-H), 7.12 (1H, dd, J = 8.8, 2.0, Ar-H), 7.30 (1H, d, J = 2.0, Ar-H), 7.43 (2H, d, J = 8.0, Ar-H), 7.71 (2H, d, J = 8.0, Ar-H), 8.97 (1H, s, CONHOH), 9.65 (1H, s, NHCO), 11.15 (1H, s, CONHOH); MS (ESI) m/z: 360 (M+H)⁺; HRMS (ESI) m/z: (M+H)⁺ calcd. for C₁₈H₂₂N₃O₅, 360.1554; found, 360.1543.

N-Hydroxy-4-{[(4-dimethylaminephenylcarbamoylmethyl)amino]methyl}benzamide (3g). A white solid, 17 mg, yield: 21.0%; m.p. 116–118 °C; ¹H-NMR: 2.83 (6H, s, 2NCH₃), 3.20 (2H, s, NHCH₂), 3.76 (2H, s, NHCH₂), 6.67 (2H, d, J = 9.2, Ar-H), 7.41 (4H, m, Ar-H), 7.70 (2H, d, J = 8.0, Ar-H), 8.97 (1H, s, NHCO), 9.50 (1H, s, CONHOH), 11.19 (1H, s, CONHOH); MS (ESI) m/z: 343 (M+H)⁺; HRMS (ESI) m/z: (M+H)⁺ calcd. for C₁₈H₂₃N₄O₃, 343.1765; found, 343.1777.

N-Hydroxy-4-{[(4-methoxybenzylcarbamoylmethyl)amino]methyl}benzamide (3h). A white solid, 31 mg, yield: 60.2%; m.p. 71–73 °C; ¹H-NMR: 3.09 (2H, s, CH₂CO), 3.69 (2H, s, NHCH₂), 3.71 (3H, s, OCH₃), 4.22 (2H, d, J = 6.0, CH₂), 6.86 (2H, d, J = 8.4, Ar-H), 7.16 (2H, d, J = 8.4, Ar-H), 7.38 (2H, d, J = 8.4, Ar-H), 7.68 (2H, d, J = 8.4, Ar-H), 8.22 (1H, t, J = 6.0, NHCO), 8.96 (1H, s, CONHOH), 11.14 (1H, s, CONHOH); MS (ESI) m/z: 344 (M+H)⁺; HRMS (ESI) m/z: (M+H)⁺ calcd. for C₁₈H₂₂N₃O₄, 344.1605; found, 344.1605.

N-Hydroxy-4-{[(phenethylcarbamoylmethyl)amino]methyl}benzamide (3i). A yellow solid, 53 mg, yield: 57.7%; m.p. 114–116 °C; ¹H-NMR: 2.72 (2H, t, J = 7.2, NHCH₂CH₂), 3.02 (2H, s, CH₂CO), 3.31 (2H, t, J = 7.2, CH₂CH₂Ph), 3.62 (2H, s, NHCH₂), 7.15–7.40 (7H, m, Ar-H), 7.68 (2H, d, J = 8.0, Ar-H), 7.84 (1H, m, NH), 8.97 (1H, s, CONHOH), 11.15 (1H, s, CONHOH); MS (ESI) m/z: 328 (M+H)⁺; HRMS (ESI) m/z: (M+H)⁺ calcd. for C₁₈H₂₂N₃O₃, 328.1656; found, 328.1651.

N-Hydroxy-4-{[(3,4-dimethoxyphenethylcarbamoylmethyl)amino]methyl}benzamide (3j). A white solid, 21 mg, yield: 37.9%; m.p. 123–125 °C. ¹H-NMR: 2.65 (2H, t, J = 7.2, NHCH₂), 3.02 (2H, s, CH₂CO), 3.30 (2H, t, J = 7.2, ArCH₂), 3.61 (2H, s, NHCH₂), 3.69 (3H, s, OCH₃), 3.71 (3H, s, OCH₃), 6.69 (1H, m, Ar-H), 6.80 (2H, m, Ar-H), 7.31 (1H, d, J = 2.0, Ar-H), 7.80 (1H, t, J = 5.6, NHCO); MS (ESI) m/z: 388 (M+H)⁺; HRMS (ESI) m/z: (M+H)⁺ calcd. for C₂₀H₂₆N₃O₅, 388.1867; found, 388.1856.

N-Hydroxy-4-{[(2-chlorophenylcarbamoylethyl)amino]methyl}benzamide (3k). A white solid, 29 mg, yield: 71.5%; m.p. 57–59 °C; ¹H-NMR: 2.48 (2H, t, J = 6.0, COCH₂CH₂), 2.79 (2H, t, J = 6.0, CH₂CH₂NH), 3.80 (2H, s, NHCH₂), 7.11 (1H, t, J = 7.6, Ar-H), 7.29 (1H, t, J = 7.6, Ar-H), 7.41 (2H, d, J = 8.0, Ar-H), 7.45 (2H, d, J = 8.0, Ar-H), 7.69 (2H, d, J = 8.0, Ar-H), 7.98 (2H, d, J = 8.0, Ar-H), 8.96 (1H, s, CONHOH), 10.51 (1H, s, CONH), 11.14 (1H, s, CONHOH); MS (ESI) m/z: 348 (M+H)⁺; HRMS (ESI) m/z: (M+H)⁺ calcd. for C₁₇H₁₉ClN₃O₃, 348.1110; found, 348.1123.

N-Hydroxy-4-{[(4-chlorophenylcarbamoylethyl)amino]methyl}benzamide (3l). A khaki solid, 47 mg, yield: 66.0%; m.p. 161–163 °C; ¹H-NMR: 2.46 (2H, t, J = 6.8, COCH₂CH₂), 2.76 (2H, t, J = 6.8, CH₂CH₂NH), 3.72 (2H, s, NHCH₂), 7.34 (4H, m, Ar-H), 7.59 (2H, d, J = 8.8, Ar-H), 7.69 (2H, d, J = 8.0, Ar-H), 10.17 (1H, s, CONH); MS (ESI) m/z: 348 (M+H)⁺; HRMS (ESI) m/z: (M+H)⁺ calcd. for C₁₇H₁₉ClN₃O₃, 348.1109; found, 348.1102.

N-Hydroxy-4-{[(4-methoxy-phenylcarbamoyl-ethyl)-amino]methyl}benzamide (3m). A white solid, 13 mg, yield: 59.9%; m.p. 173–175 °C; ¹H-NMR: 2.43 (2H, t, J = 6.4, COCH₂CH₂), 2.76 (2H, t, J = 6.4, CH₂CH₂NH), 3.71 (3H, s, OCH₃), 3.75 (2H, s, NHCH₂), 6.85 (2H, d, J = 9.2, Ar-H), 7.39 (2H, d, J = 8.0, Ar-H), 7.47 (2H, d, J = 9.2, Ar-H), 7.69 (2H, d, J = 8.0, Ar-H), 8.97 (1H, s, CONHOH), 9.87 (1H, s, CONH), 11.14 (1H, s, CONHOH); MS (ESI) m/z: 344 (M+H)⁺; HRMS (ESI) m/z: (M+H)⁺ calcd. for C₁₈H₂₂N₃O₄, 344.1605; found, 344.1594.

N-Hydroxy-4-{[(3,4-dimethoxyphenylcarbamoylethyl)amino]methyl}benzamide (3n). A white solid, 26 mg, yield: 44.3%; m.p. 175–176 °C; ¹H-NMR: 2.72 (2H, t, J = 7.2, NHCH₂CH₂), 3.02 (2H, s, CH₂CO), 3.31 (2H, t, J = 7.2, CH₂CH₂Ph), 3.62 (2H, s, NHCH₂), 7.15–7.40 (7H, m, Ar-H), 7.68 (2H, d, J = 8.0, Ar-H), 7.84 (1H, m, NH), 8.97 (1H, s, CONHOH), 11.15 (1H, s, CONHOH); MS (ESI) m/z: 374 (M+H)⁺; HRMS (ESI) m/z: (M+H)⁺ calcd. for C₁₉H₂₄N₃O₅, 374.1711; found, 374.1713.

N-Hydroxy-4-{[(4-methoxybenzylcarbamoylethyl)amino]methyl}benzamide (3o). A khaki solid, 32 mg, yield: 52.9%; m.p. 152–154 °C; ¹H-NMR: 2.28 (2H, t, J = 6.8, COCH₂CH₂), 2.69 (2H, t, J = 6.8, CH₂CH₂NH), 3.70 (2H, s, NHCH₂), 3.71 (3H, s, OCH₃), 4.17 (2H, d, CH₂NHCO), 6.84 (2H, d, J = 8.8, Ar-H), 7.15 (2H, d, J = 8.8, Ar-H), 7.34 (2H, d, J = 8.0, Ar-H), 7.68 (2H, d, J = 8.0, Ar-H), 8.32 (1H, t, J = 5.6, CONH),8.96 (1H, s, CONHOH), 11.14 (1H, s, CONHOH); MS (ESI) m/z: 358 (M+H)⁺; HRMS (ESI) m/z: (M+H)⁺ calcd. for C₁₉H₂₄N₃O₄, 358.1761; found, 358.1771.

3.6. CLA-1 Up-Regulating Activity

CLA-1 up-regulating activity was analyzed as described previously [14]. Briefly, HepG2 cells stably transfected with pGL3-CLAP containing CLA-1 promoter region were seeded in 96-well plates at 5 × 10⁴ number/well in MEM (Hyclone, Logan, UT, USA) (100 μL) containing 10% FBS (Hyclone) and 600 μg/mL G418 (Invitrogen, Carlsbad, CA, USA). With ~80% confluence, the cells were washed once with PBS (pH 7.3, 137 mM NaCl, 2.7 mM KCl, 4.3 mM Na₂HPO₄, 1.4 mM KH₂PO₄), followed by incubation with MEM (200 μL) containing 0.1% vehicle (DMSO), positive control (2.5 μM SAHA or 3.0 μM TSA) or 10 μg/mL compounds. After 18 h incubation at 37 °C, cells were washed with PBS, and then the luciferase activity was detected using the Luciferase Assay System (Promega, Madison, WI, USA). EC₅₀ values of the compounds were determined by a dose-response assay. Briefly, the above mentioned HepG2 cells were treated with the indicated concentrations of the compounds and detected by luciferase assay. The does-response curves were obtained and the apparent EC₅₀ value for each compound was calculated using Sigma Plot 9.0.

3.7 Analysis for Cell Surface Expression by Flow Cytometry

Cell surface expression of CLA-1 was analyzed by flow cytometry as described previously [7]. Briefly, HepG2 cells were plated in 24-well dishes at 50,000 cells/well, followed by treatment for 24 h with 0.3 μM compounds or vehicle (0.1% DMSO). Then HepG2 cells were trypsinized from the plate, washed and resuspended in 4% paraform fixing solution, incubated overnight at 4 °C. After fixing, cells were blocked for 15 min at 4 °C in PBS containing 5% FBS. Cells were then incubated with monoclonal antibody to CLA-1 (BD Biosciences, San Jose, CA, USA) at a final dilution of 1:50 (4 °C, 1 h), followed by washing and staining with FITC-conjugated goat antibody to mouse IgG (1:100 dilution, 4 °C, 1 h). The cell suspension was centrifuged (800 × g, 3 min, 4 °C), the pellet was resuspended in PBS, and fluorescence intensity was analyzed using a BD FACSCalibur flow cytometer (BD Biosciences).

3.8 Analysis of Cellular Uptake of DiI-labeled HDL by Flow Cytometry

For the cellular DiI-HDL uptake by HepG2 cells assays, the cell pretreatment was the same as for the cell surface expression assay. Cells were incubated with DiI-HDL (2 μg/mL) and 0.3 μM compounds or vehicle for 12 h at 37 °C, then washed with PBS and incubated with PBS containing 0.5% bovine serum albumin (BSA) and 2 mM EDTA for 1 h at 4 °C, detached from the plate by gentle pipetting. The cell suspension was centrifuged (3 min, 800 g, 4 °C), the obtained pellet was resuspended in PBS, and DiI fluorescence was analyzed using a BD FACSCalibur flow cytometer (BD Biosciences).

4. Conclusions

A small library of hydroxamic acid compounds was constructed and the compounds were assessed for their up-regulating activity on CLA-1 expression in HepG2 cells. Among them, compound 1g showed the best potency, with EC₅₀ = 0.32 μM.