Inhibition of Inflammatory Mediators by Neobavaisoflavone in Activated RAW264.7 Macrophages
Abstract
:1. Introduction
2. Results and Discussion
Inflammatory mediator | ED50 [μM] |
---|---|
NO (nitrite) | 25.0 |
IL-1β | 23.11 |
IL-6 | 5.03 |
IL-12p40 | 5.23 |
IL-12p70 | 5.26 |
TNF-α | 18.80 |
3. Experimental
3.1. General
3.2. Cell Culture
3.3. Cell Viability Assay
3.4. Lactate Dehydrogenase Release Assay
3.5. Quantification of Nitric oxide Production
3.6. Detection of ROS and RNS Production by Chemiluminescence
3.7. Multiplex Bead-Based Cytokine Assay
3.8. Statistical Analysis
4. Conclusions
Acknowledgements
References and Notes
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Szliszka, E.; Skaba, D.; Czuba, Z.P.; Krol, W. Inhibition of Inflammatory Mediators by Neobavaisoflavone in Activated RAW264.7 Macrophages. Molecules 2011, 16, 3701-3712. https://doi.org/10.3390/molecules16053701
Szliszka E, Skaba D, Czuba ZP, Krol W. Inhibition of Inflammatory Mediators by Neobavaisoflavone in Activated RAW264.7 Macrophages. Molecules. 2011; 16(5):3701-3712. https://doi.org/10.3390/molecules16053701
Chicago/Turabian StyleSzliszka, Ewelina, Dariusz Skaba, Zenon P. Czuba, and Wojciech Krol. 2011. "Inhibition of Inflammatory Mediators by Neobavaisoflavone in Activated RAW264.7 Macrophages" Molecules 16, no. 5: 3701-3712. https://doi.org/10.3390/molecules16053701