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Article

Synthesis and Biological Activity Evaluation of Novel β-Substituted Nitromethylene Neonicotinoid Analogues

Shanghai Key Laboratory of Chemical Biology, School of Pharmacy, East China University of Science and Technology, P.O. Box 544, 130 Meilong Road, Shanghai 200237, China
*
Author to whom correspondence should be addressed.
Molecules 2012, 17(9), 10014-10025; https://doi.org/10.3390/molecules170910014
Submission received: 25 June 2012 / Revised: 13 August 2012 / Accepted: 14 August 2012 / Published: 24 August 2012

Abstract

:
The structure-based design and synthesis of a series of novel neonicotinoid analogues are described. The novel neonicotinoid analogues were designed based upon the reaction of enamine derivatives with electron-withdrawing β-substituents with electrophilic thiocyanogen reagents. These compounds were characterized by spectroscopic methods. Bioassays indicated that some of the synthesized compounds exhibited excellent bioactivity against cowpea aphids (Aphis craccivora). The LC50 values of compounds 7, 9, 12, 13, 15, 17, 19, 20 and commercial imidacloprid were 0.01567, 0.00974, 0.02494, 0.01893, 0.02677, 0.01778, 0.0220, 0.02447 and 0.03502 mmol L−1, respectively, which suggested that they could be used as leads for future development of new insecticides.

1. Introduction

In recent years, neonicotinoid insecticides have been the fastest growing class of insecticides in modern crop protection, with widespread use against a broad spectrum of sucking and certain chewing pests [1,2,3,4,5]. Significant progress had been obtained on the chemistry of the insecticidal nicotinic agonists and the chemical biology of the nicotinic acetylcholine receptors (nAChRs), that is, chemorational approaches including physicochemical considerations, metabolism, resistance mechanisms, and chemical and structural biology aspects potentially expediting receptor structure-guided insecticide design [6,7,8,9,10,11,12].
Our past endeavors in this field have focused on systematic pharmacophore modifications of nitromethylene neonicotinoids that appeared to capture the “bioactive” conformation (Figure 1). Several types of nitromethylene neonicotinoids with the cis-configuration were presented; that is, neonicotinoids 1 with a tetrahydropyridine fixed cis-configurations; neonicotinoids 2 with bulky group fixed cis-configurations, neonicotinoids 3 with cis-configuration constructed by aza-Diels-Alder reactions, and divalent and oxa-bridged neonicotinoids 4 constructed from dialdehydes (Figure 1). Bioassays indicated that the synthesized cis compounds 14 exhibited excellent bioactivity against cowpea aphids [13,14,15,16]. According to our knowledge, the nitro groups in all commercialized neonicotinoids have a trans configuration, on which three proposals for mode of action are based [17]. The high bioactivity of compounds 14 implies that neonicotinoids in the cis configuration might bind to the receptor in a different way [13]. These efforts accordingly encourage the discovery of “superneonicotinoids” effective for imidacloprid-resistant pests and lepidoptera species.
Figure 1. .Structures of imidacloprid neonicotinoids and structurally related analogues.
Figure 1. .Structures of imidacloprid neonicotinoids and structurally related analogues.
Molecules 17 10014 g001
The structure derivative method through the organic reaction is another important tool for systematic pharmacophore modifications [18]. It had been theorized that the nitroenamine entities in these high active compounds play important roles for specific binding to the desired insect nAChRs site [19,20,21,22]. Encouraged by these reports, the modifications of nitroenamine entities to fix its configuration through organic reactions has attracted our attention. The chemistry of enamines, including push–pull enamines bearing an additional electron-acceptor group at the β-position, has been extensively developed, mainly as a result of electrophilic reactions that proceed predominantly at the β-position [23,24,25]. Electrophilic modification, followed by a set of transformations, have made enamines an integral part of synthetic organic chemistry methodology [26]. Additionally, electron-acceptor groups can participate in chemical transformations. According to this knowledge, a series of prototype compounds with extended N-substituted (thiocyanogen) substituents were designed in this paper (Figure 2). The choice of the thiocyanogen was predominantly governed by its traits as electrophilic reagent, and mainly by its ability to easily cyclize with the NH group in the imidazoline ring to fix the electron-withdrawing group in the cis position (Figure 2) [27].
Figure 2. .Structures of imidacloprid neonicotinoids and its structurally related analogues.
Figure 2. .Structures of imidacloprid neonicotinoids and its structurally related analogues.
Molecules 17 10014 g002

2. Results and Discussion

2.1. Synthetic Chemistry

The synthetic route of the target compounds is summarized in Scheme 1. The chemical structures of the novel neonicotinoid analogues under study are depicted in Figure 3. The preparation of the designed compounds began with the common intermediates 5ah and 6ac which were synthesized according to the method of Kagabu et al. [19]. In the reaction of 5ah with thiocyanogen at 0 °C for 1.5 h, the corresponding β-thiocyanated products 714 were produced in 63%–82% yields. In this procedure, 5a appears to produce a mixture of 7 and cyclization product 15. Similarly, 5c and 5f also gave mixtures of aminothiazole and the relevant cyclization product. Compounds 7, 9 and 12 were synthesized by reducing the reaction time and reaction temperature without forming cyclization products. Then, these compounds 79 and 1113 were converted quantitatively into the corresponding cyclization products, 1520, by stirring at room temperature in dichloromethane solution containing 0.1% piperidine as catalyst. Unexpectedly, 10 and 14 could not be converted into the corresponding cyclization products. The substrates 6ac yielded only the corresponding cyclization products 2123 respectively under the same conditions. Whereas configuration of compounds 714 and 2123 cannot be determined easily by standard 1H- and 13C-NMR techniques, the compounds 1520 obtained from the reaction system are considered to be produced with retention of the configuration of the preformed enamines with electron-withdrawing β-substituents, followed by catalyzed cyclization. The structures of the title compounds were well characterized by 1H-NMR, 13C-NMR and HRMS.
Scheme 1. .Synthetic route for the title compounds 723.
Scheme 1. .Synthetic route for the title compounds 723.
Molecules 17 10014 g004
Figure 3. Chemical structures of novel neonicotinoid analogues under study.
Figure 3. Chemical structures of novel neonicotinoid analogues under study.
Molecules 17 10014 g003

2.2. Biological Activities

The insecticidal activities of the designed compounds against cowpea aphids are shown in Table 1. Most of the title compounds showed excellent insecticidal activities against cowpea aphids. For example, the LC50 values of compounds 7, 9, 12, 13, 15, 17, 19, and 20 were 0.01567, 0.00974, 0.02494, 0.01893, 0.02677, 0.01778, 0.0220 and 0.02447 mmol L−1, respectively, whereas that of imidacloprid was only 0.03502 mmol L−1 [16]. Activities varied significantly depending upon the patterns of substituents on the enamine and imidazole ring. It was observed that compounds with NO2 substituents (7, 15, 9 and 17) demonstrated better activities than that with CN substituents (8, 10 and 16). Generally, the imidazolidine substituents appear to be the important factor for insecticidal activities. A benzene substituent (compound 14) resulted in complete inactivity. Moreover, the cyclic addition of SCN substituents with the imidazolidine ring is unfavourable for high activities from the data analysis present of compounds 7, 9, 15 and 17.
Table 1. .Insecticidal activities of compounds 723 against cowpea aphids (Aphis craccivora).
Table 1. .Insecticidal activities of compounds 723 against cowpea aphids (Aphis craccivora).
CompoundsMortality (%, 500mg L−1)LC50 (mmol L−1)
71000.01567
834.6nt a
91000.00974
100nt
1123.98nt
121000.02494
131000.01893
1487.82nt
151000.02677
1632.4nt
171000.01778
1816.19nt
191000.02200
201000.02447
2157.6nt
221000.11839
2350.79nt
a Not tested.

3. Experimental

3.1. General

Melting points (mp) were recorded on a Büchi B540 apparatus (Büchi Labortechnik AG, Flawil, Switzerland) and are uncorrected. 1H-NMR and 13C-NMR spectra were recorded on a Bruker AM-400 (400 MHz) spectrometer with CDCl3 or DMSO-d6 as the solvent and TMS as the internal standard. Chemical shifts are reported in δ (parts per million) values. High-resolution mass spectra were recorded under electron impact (70 eV) condition using a MicroMass GCT CA 055 instrument. Analytical thin-layer chromatography (TLC) was carried out on precoated plates (silica gel 60 F254), and spots were visualized with ultraviolet (UV) light. The compounds 5ah and 6ac were synthesized according to the method of Kagabu et al. [19]. Unless otherwise noted, reagents and solvents were used as received from commercial suppliers. Yields were not optimized. All reactions were carried out under a protective atmosphere of drying nitrogen or utilizing a calcium chloride drying tube.

3.1.1. General Synthetic Procedure for 7–14

To a solution of 5ag (10 mmol) in dichloromethane (100 mL), a thiocyanogen (10 mmol) solution in dichloromethane (50 mL), which was previously prepared from lead thiocyanate and bromine at −15 °C, was added dropwise with stirring at −15 °C and then the mixture was stirred for 1.5 h at 0 °C. The reaction mixture was washed with cold water (3 × 50 mL) and brine (50 mL), and dried with sodium sulfate. The solvent was removed to give a crude solid, which was recrystallized from a mixture of dichloromethane/ether (2:1, v/v) to give 714.
2-Chloro-5-((2-(nitro(thiocyanato)methylene)imidazolidin-1-yl)methyl)pyridine (7). Yield: 92%, m.p.: 130.6–132.1 °C; 1H-NMR (DMSO-d6): δ 3.76 (br.s, 4H), 4.77 (s, 2H), 7.55 (d, J = 8.4 Hz, 1H), 7.86 (dd, J1 = 2.6 Hz, J2 = 8.4 Hz, 1H), 8.42 (d, J = 2.0 Hz, 1H), 9.43 (s, 1H) ppm; 13C-NMR (DMSO-d6): δ 162.6, 149.9, 149.1, 139.1, 131.8, 124.7, 112.3, 87.5, 50.7, 50.2, 42.7; HRMS (ES+) calcd for C11H1035ClN5O2S (M+H)+: 312.0244. Found: 312.0330. Calcd for C11H1037ClN5O2S (M+H)+: 314.0214 Found: 314.0284.
2-(1-((6-Chloropyridin-3-yl)methyl)imidazolidin-2-ylidene)-2-thiocyanatoacetonitrile (8). Yield: 63%, m.p.: 118.2–119.7 °C; 1H-NMR (DMSO-d6): δ 3.51–3.56 (m, 2H), 3.66–3.70 (m, 2H), 4.79 (s, 2H), 7.54 (d, J = 8.4 Hz, 1H), 7.78–7.80 (m, 1H), 8.11 (s, 1H), 8.36 (d, J = 2.4 Hz, 1H) ppm; 13C-NMR (DMSO-d6): δ 164.9, 150.0, 149.2, 139.2, 132.1, 124.8, 122.1, 113.8, 51.6, 47.4, 41.5, 31.2; HRMS (EI+) calcd for C12H1035ClN5S (M+): 291.0345. Found: 291.0344. Calcd for C12H1037ClN5S (M+): 293.0316 Found: 293.0318.
1-((2-Chlorothiazol-5-yl)methyl)-2-(nitro(thiocyanato)methylene)imidazolidine (9). Yield: 75%, m.p.: 85.1–86.3 °C; 1H-NMR (DMSO-d6): δ 3.69–3.78 (m, 4H), 4.84 (s, 2H), 7.73 (s, 1H), 9.40 (s, 1H) ppm; 13C-NMR (DMSO-d6): δ 162.2, 151.6, 141.8, 135.9, 112.1, 87.3, 50.1, 46.3, 42.6; HRMS (ES+) calcd for C9H835ClN5O2S2 (M+H)+: 317.9808. Found: 317.9890. Calcd for C9H837ClN5O2S2 (M+H)+: 319.9778 Found: 319.9860.
2-(1-((2-Chlorothiazol-5-yl)methyl)imidazolidin-2-ylidene)-2-thiocyanatoacetonitrile (10). Yield: 52%, m.p.: 120.3–122.2 °C; 1H-NMR (DMSO-d6): δ 3.30–3.70 (m, 4H), 4.93 (s, 2H), 7.70 (s, 1H), 8.17 (s, 1H) ppm; 13C-NMR (DMSO-d6): δ 164.5, 151.3, 141.6, 135.8, 122.0, 113.5, 51.0, 43.0, 41.5, 31.8; HRMS (ES−) calcd for C10H835ClN5S (M−H)+: 295.9910. Found: 295.9829. Calcd for C10H837ClN5S (M−H)+: 297.9880 Found: 297.9803.
1-Benzyl-2-(nitro(thiocyanato)methylene)imidazolidine (11). Yield: 82%, m.p.: 102.3–104.2 °C; 1H-NMR (DMSO-d6): δ 3.72 (s, 4H), 4.75 (s, 2H), 7.32–7.42 (m, 5H), 9.40 (s, 1H) ppm; 13C-NMR (DMSO-d6): δ 162.3, 136.1, 129.2, 128.1, 127.6, 112.3, 86.9, 52.7, 50.3, 42.6; HRMS (ES+) calcd for C12H12N4O2S (M+H)+: 277.0681. Found: 277.0750.
1-((6-Chloropyridin-3-yl)methyl)-2-(nitro(thiocyanato)methylene)-hexahydropyrimidine (12). Yield: 70%, m.p.: 158.1–160.3 °C; 1H-NMR (DMSO-d6): δ 2.14 (t, J = 5.2, 2H), 3.38 (t, J = 5.6 Hz, 2H), 3.85 (t, J = 5.6 Hz, 2H), 4.06 (s, 2H), 7.57 (d, J = 8.0 Hz, 1H), 7.91 (dd, J1 = 8.4 Hz, J2 = 2.8 Hz, 1H), 8.45 (d, J = 2.4 Hz, 1H) ppm; 13C-NMR (DMSO-d6): δ 164.7, 158.9, 150.2, 146.2, 140.1, 131.0, 124.5, 106.5, 56.8, 47.2, 45.1, 19.1; HRMS (ES+) calcd for C12H1235ClN5O2S (M+H)+: 326.0400. Found: 326.0477. Calcd for C12H1237ClN5 O2S (M+H)+: 328.0371 Found: 328.0465.
5-((2-(Nitro(thiocyanato)methylene)imidazolidin-1-yl)methyl)-2-(trifluoromethyl)pyridine (13). Yield: 82%, m.p.: 140.1–142.3 °C; 1H-NMR (DMSO-d6): δ 3.76 (s, 4H), 3.38 (t, J = 5.6 Hz, 2H), 4.85 (s, 2H), 7.91 (d, J = 8.0 Hz, 1H), 8.04 (s, 1H), 8.75 (s, 1H), 9.45 (s, 1H) ppm; 13C-NMR (DMSO-d6): δ 162.7, 149.3, 137.2, 136.6, 121.1, 112.3, 87.7, 50.9, 50.8, 42.8; HRMS (ES+) calcd for C12H10F3N5O2S (M+H)+: 345.0507. Found: 345.0617.
(E-1-((6-Chloropyridin-3-yl)methyl)-2-(nitro(thiocyanato)methylene)-2,3-dihydro-1H-enzo[d]imid-azole (14). Yield: 74%, m.p.: 125.6–127.2 °C; 1H-NMR (DMSO-d6): δ 4.79 (s, 2H), 7.39–7.7 (m, 6H), 8.35 (d, J = 2.4 Hz, 1H) ppm; 13C-NMR (DMSO-d6): δ 150.1, 148.7, 148.6, 138.5, 132.5, 131.3, 130.4, 126.0, 125.4, 124.8, 113.8, 112.2, 112.0, 86.0, 55.4, 47.2; HRMS (ES−) calcd for C10H835ClN5S (M−H)+: 358.0244. Found: 358.0130. Calcd for C10H837ClN5S (M−H)+: 360.0214. Found: 297.0166.

3.1.2. General Synthetic Procedure for 15–20

The solution of 79 and 1113 (10 mmol) in dichloromethane (50 mL) containing 0.1% piperidine was stirred for 2 h at room temperature. After removal of the solvent, the residue was recrystallized from dichloromethane to give 1520 in quantitative yields.
1-((6-Chloropyridin-3-yl)methyl)-7-nitro-2,3-dihydroimidazo[1,2-c]thiazol-5(1H)-imine (15). Yield: 93%, m.p.: 136.4–138.5 °C; 1H-NMR (DMSO-d6): δ 3.85 (s, 2H), 4.08 (s, 2H), 5.27 (s, 2H), 7.58 (br.s, 1H), 7.91–7.92 (m, 1H), 8.47 (s, 1H), 8.91 (br.s, 1H) ppm; 13C-NMR (DMSO-d6): δ 150.6, 150.1, 149.7, 139.8, 132.0, 124.8, 99.7, 55.7, 49.4, 41.8; HRMS (ES+) calcd for C11H1035ClN5O2S (M+H)+: 312.0244. Found: 312.0321. Calcd for C11H1037ClN5O2S (M+H)+: 314.0279. Found: 314.0284.
1-((6-Chloropyridin-3-yl)methyl)-5-imino-1,2,3,5-tetrahydroimidazo[1,2-c]thiazole-7-carbonitrile (16). Yield: 82%, m.p.: 201.5–202.3 °C; 1H-NMR (DMSO-d6): δ 3.87 (s, 4H), 4.61 (s, 2H), 7.41 (d, J = 8.0 Hz, 1H), 7.73–7.76 (m, 1H), 8.38 (d, J = 2.0 Hz, 1H) ppm; 13C-NMR (DMSO-d6): δ 155.2, 153.0, 152.0, 149.3, 138.9, 129.1, 125.0, 114.7, 53.2, 47.8, 47.2, 41.0; HRMS (ES+) calcd for C12H1035ClN5S (M+H)+: 292.0345. Found: 292.0430. Calcd for C12H1037ClN5S (M+H)+: 294.0316. Found: 294.0410.
1-((2-Chlorothiazol-5-yl)methyl)-7-nitro-2,3-dihydroimidazo[1,2-c]thiazol-5(1H)-imine (17). Yield: 78%, m.p.: 150.3–151.4 °C; 1H-NMR (DMSO-d6): δ 3.81–4.16 (m, 4H), 5.34 (s, 2H), 7.77 (s, 1H), 8.96 (s, 1H) ppm; 13C-NMR (DMSO-d6): δ 152.3, 150.0, 142.0, 136.0, 99.9, 55.5, 45.2, 41.7; HRMS (EI+) calcd for C9H8ClN5O2S2 (M+): 316.9808. Found: 316.9807.
1-Benzyl-7-nitro-2,3-dihydroimidazo[1,2-c]thiazol-5(1H)-imine (18). Yield: 74%, m.p.: 82.3–84.4 °C; 1H-NMR (DMSO-d6): δ 3.81–4.06 (m, 4H), 5.26 (s, 2H), 7.32–7.43 (s, 1H), 8.96 (s, 1H) ppm; 13C-NMR (DMSO-d6): δ 150.2, 136.4, 129.2, 128.3, 55.5, 52.3, 41.6; HRMS (ES+) calcd for calcd for C12H12N4O2S (M+H)+: 277.0681. Found: 277.0746.
1-((6-Chloropyridin-3-yl)methyl)-8-nitro-1,2,3,4-tetrahydrothiazolo[3,4-a]pyrimidin-6-imine (19). Yield: 65%, m.p.: 165.3–167.4 °C; 1H-NMR (DMSO-d6): δ 2.01 (t, J = 5.2 Hz, 2H), 3.30 (t, J = 5.6 Hz, 2H), 3.73 (t, J = 5.6 Hz, 2H), 4.90 (s, 2H), 7.53 (d, J = 8.4 Hz, 1H), 7.92 (dd, J1 = 8.4 Hz, J2 = 2.4 Hz, 1H), 8.43 (d, J = 2.0, 1H), 9.19 (s, 1H) ppm; 13C-NMR (DMSO-d6): δ 153.5, 150.5, 150.0, 149.8, 139.9, 131.9, 124.4, 103.2, 56.5, 47.3, 41.6, 19.3; HRMS (ES+) calcd for C12H1235ClN5O2S (M+H)+: 326.0400. Found: 326.0476. Calcd for C12H1237ClN5O2S (M+H)+: 328.0371. Found: 328.0473.
7-Nitro-1-((6-(trifluoromethyl)pyridin-3-yl)methyl)-2,3-dihydroimidazo[1,2-c]thiazol-5(1H)-imine (20). Yield: 88%, m.p.: 148.2–149.3 °C; 1H-NMR (DMSO-d6): δ 3.85 (t, J = 8.2 Hz, 2H), 4.10 (t, J = 9.2 Hz, 2H), 5.36 (s, 2H), 7.94 (d, J = 8.4 Hz, 1H), 8.13 (d, J = 8.4 Hz, 1H), 8.81(s, 1H), 8.94 (s, 1H) ppm; 13C-NMR (DMSO-d6): δ 151.0, 149.8, 137.8, 136.8, 121.1, 56.0, 50.0, 41.8; HRMS (ES+) calcd for C12H10F3N5O2S (M+H)+: 346.0507. Found: 346.0634.

3.1.3. General Synthetic Procedure for 21–23

To a solution of 6ac (10 mmol) in dichloromethane (50 mL) was added with stirring at −15 °C a dichloromethane solution of thiocyanogen (10 mmol) which had previously prepared from lead thiocyanate and bromine at −15 °C, and then the mixture was stirred for 1.5 h at room temperature. The reaction mixture was washed with cold water (3 × 50 mL) and brine (50 mL) and then dried with sodium sulfate. The solvent was evaporated in vacuo, and fractional precipitation of the residue from dichloromethane afforded 2123.
N-((6-Chloropyridin-3-yl)methyl)-N-ethyl-2-imino-3-methyl-5-nitro-2,3-dihydrothiazol-4-amine (21). Yield: 84%, m.p.: 120.3–121.4 °C; 1H-NMR (DMSO-d6): δ 1.14 (t, J =7.2 Hz, 3H), 3.17 (d, J = 7.2 Hz, 2H), 3.40 (s, 3H), 4.57 (s, 2H), 7.54 (d, J = 8.0 Hz, 1H), 7.92–7.95 (m, 1H), 8.49 (d, J = 2.0 Hz, 1H) ppm; 13C-NMR (DMSO-d6): δ 165.1, 150.7, 150.5, 140.9, 131.8, 124.8, 118.2, 51.9, 46.8, 36.1, 13.9; HRMS (EI+) calcd for C12H14ClN5O2S (M+): 327.0557. Found: 327.0559.
N-((6-Chloropyridin-3-yl)methyl)-2-imino-3-methyl-5-nitro-2,3-dihydrothiazol-4-amine (22). Yield: 82%, m.p.: 116.4–118.4 °C; 1H-NMR (DMSO-d6): δ 3.28 (s, 3H), 5.37 (s, 2H), 7.80 (s, 1H) ppm; 13C-NMR (DMSO-d6): δ 152.1, 142.5, 141.2, 42.5, 34.6, 33.3; HRMS (ES−) calcd for C8H835ClN5O2S2(M−H)+: 303.9808. Found: 303.9716. calcd for C8H837ClN5O2S2 (M−H)+: 305.9778. Found: 305.9703.
N-((6-Chloropyridin-3-yl)methyl)-3-ethyl-2-imino-5-nitro-2,3-dihydrothiazol-4-amine (23). Yield: 78%, m.p.: 115.4–117.2 °C; 1H-NMR (DMSO-d6): δ 1.23 (t, J = 7.2 Hz, 3H), 3.66 (q, J = 7.2 Hz, 2H), 5.34 (s, 2H), 7.81 (s, 1H); 13C-NMR (DMSO-d6): δ 152.1, 141.2, 134.7, 42.7, 42.1, 15.7; HRMS (ES−) calcd for C9H1035ClN5O2S2 (M-H)+: 317.9964. Found: 317.9870. calcd for C9H1037ClN5O2S2 (M−H)+: 319.9935. Found: 319.9836.

3.2. Biological Assay

All compounds were dissolved in acetone and diluted with water containing Triton X-100 (0.1 mg L−1) to obtain series concentrations of 500.0, 250.0, 125.0 mg L−1 and others for bioassays. Pea aphids (Aphis craccivora) were dipped according to our previously reported procedure [13,23]. Tender shoots of soybean with 40-60 healthy apterous adults were dipped in diluted solutions of the chemicals containing Triton X-100 (0.1 mg L−1) for 5 s, the superfluous fluid was removed, and the shoots were placed in the conditioned room (25 ± 1 °C, 50% RH). Water containing Triton X-100 (0.1 mg L−1) was used as control. Mortality was assessed after 24 h. Each treatment had three repetitions, and the data were adjusted and subjected to probit analysis as before.

4. Conclusions

In conclusion, a series of a novel class of neonicotinoids, in which the common nitromethylene pharmacophore was substituted by electrophilic thiocyanogen reagents, were designed and synthesized. Most of the compounds exhibited excellent insecticidal activities against cowpea aphids (A. craccivora), which implied that reaction of nitromethylene with the electrophilic system to obtain novel neonicotinoids analogues with high activities was feasible.

Acknowledgments

The work was financial supported by National Basic Research Program of China (973 Program, 2010CB126100), National High Technology Research Development Program of China (863 Program, 2011AA10A207), Key Projects in the National Science & Technology Pillar Program during the Twelfth Five-Year Plan Period (2011BAE06B05). National Natural Science Foundation of China (21002030), Natural Science Foundation of Shanghai (10ZR1407300), Shanghai Education Committee (12ZZ057) and the Fundamental Research Funds for the Central Universities. This work was also partly supported Shanghai Leading Academic Discipline Project, Project Number: B507 and Australia DC Foundation.

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  • Sample Availability: Samples of the title compounds are available from the authors.

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MDPI and ACS Style

Wang, B.; Cheng, J.; Xu, Z.; Xu, X.; Shao, X.; Li, Z. Synthesis and Biological Activity Evaluation of Novel β-Substituted Nitromethylene Neonicotinoid Analogues. Molecules 2012, 17, 10014-10025. https://doi.org/10.3390/molecules170910014

AMA Style

Wang B, Cheng J, Xu Z, Xu X, Shao X, Li Z. Synthesis and Biological Activity Evaluation of Novel β-Substituted Nitromethylene Neonicotinoid Analogues. Molecules. 2012; 17(9):10014-10025. https://doi.org/10.3390/molecules170910014

Chicago/Turabian Style

Wang, Baozhu, Jiagao Cheng, Zhiping Xu, Xiaoyong Xu, Xusheng Shao, and Zhong Li. 2012. "Synthesis and Biological Activity Evaluation of Novel β-Substituted Nitromethylene Neonicotinoid Analogues" Molecules 17, no. 9: 10014-10025. https://doi.org/10.3390/molecules170910014

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