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Article

Synthesis and Characterization of Some New Complexes of Magnesium (II) and Zinc (II) with the Natural Flavonoid Primuletin

by
Valentina Uivarosi
1,*,
Mihaela Badea
2,†,
Rodica Olar
2,†,
Constantin Drǎghici
3,† and
Ştefania Felicia Bǎrbuceanu
4
1
Department of General and Inorganic Chemistry, Faculty of Pharmacy, Carol Davila University of Medicine and Pharmacy, 6 Traian Vuia St, Bucharest 020956, Romania
2
Department of Inorganic Chemistry, Faculty of Chemistry, University of Bucharest, 90-92 Panduri St., Bucharest 050663, Romania
3
Center of Organic Chemistry C.D. Nenitzescu, Romanian Academy, 202 B Splaiul Independenţei, Bucharest 060023, Romania
4
Department of Organic Chemistry, Faculty of Pharmacy, Carol Davila University of Medicine and Pharmacy, 6 Traian Vuia St, Bucharest 020956, Romania
*
Author to whom correspondence should be addressed.
†
These authors contributed equally to this work.
Molecules 2013, 18(7), 7631-7645; https://doi.org/10.3390/molecules18077631
Submission received: 20 May 2013 / Revised: 17 June 2013 / Accepted: 19 June 2013 / Published: 1 July 2013
Browse Figures
Versions Notes

Abstract

:
Two new metal complexes formulated as [Mg(L)2(H2O)2]·H2O (1) and [Zn(L)2(H2O)2]·0.5H2O (2), where HL = 5-hydroxyflavone (primuletin), have been synthesized and characterized by elemental and thermal analyses, molar conductance, IR, UV-Vis, 1H- and 13C-NMR, fluorescence and mass spectra. In solid state, complexes had shown higher fluorescence intensities comparing to the free ligand, and this behavior is appreciated as a consequence of the coordination process.

Graphical Abstract

1. Introduction

Primuletin (5-hydroxyflavone, 5-hydroxy-2-phenyl-4H-1-benzopyran-4-one, Figure 1) is a naturally occurring flavone, widely distributed in plants belonging to the Primula and Dionisya species [1,2].
Molecules 18 07631 g001 550
Figure 1. The structure of primuletin (5-hydroxyflavone).
Figure 1. The structure of primuletin (5-hydroxyflavone).
Molecules 18 07631 g001
As for other flavonoid compounds, the antioxidant properties as well as the effects on cell lines, receptors or enzymes have attracted a major interest in order to correlate them with biological activity.
The antioxidant activity of flavonoids is generally associated with three chemical features: (i) an ortho-dihydroxy structure in the B-ring, (ii) the presence of a 2,3 double bond in the C-ring, and/or (iii) the presence of a 4-oxo function in the C-ring [3]. Additionally, an OH group in position 3 of the C-ring was correlated with the antioxidant properties [4]. In the case of 5-hydroxyflavone, only two of these necessary conditions are met, and consequently it has not noticeable antioxidant activity [5,6]. The monoxydroxylated primuletin has a low or absent scavenging effect [7,8] correlated with a low cytotoxicity on B 16 melanoma cells, and induced little apoptosis in human leukemia cells [9] or even had not apoptotic effect [10].
On the other side, 5-hydroxyflavone can act as activator of calcium-activate and ATP-sensitive potassium channels; the hydroxyl group in position 5 seems to be a structural requirement for a possible interaction with calcium-activated potassium channels [11]. As consequence, 5-hydroxyflavone showed full vasorelaxing effects in a comparative study involving 17 different flavones.
From a series of 25 tested flavones, 5-hydroxyflavone proved to be had the highest androgen receptor (AR) antagonistic activity. Its action was threefold higher than that of flutamide, a well known AR antagonist used to treat prostate cancer [12].
The presence of a hydroxy group in position 5 confers to primuletin a superior activity on some enzymes comparing to unhydroxylated or methoxylated analogues. In this regard, the C(5)-hydroxyl group induce a better inhibition of phospholipase A2 group II A (PLA2–IIA) than their respective C(5)-unhydroxylated derivatives [13]. The inhibition of PLA2 leads to a decrease in eicosanoids levels, thereby reducing inflammation. The presence of OH function in C-5 position is responsible for the much higher activity of 5-hydroxyflavone comparing to 5-methoxyflavone in inhibition of nuclear factor κB (NF-κB) [14]. Since NF-κB is involved in inflammation, cell proliferation, apoptosis and angiogenesis [15], the molecules that interfere with NF-κB signaling may be useful as anti-inflammatory and anticancer agents.
Primuletin inhibits nitric oxide syntase-2 (NOS-2) induced in macrophages by lipopolysaccaride (LPS) from Escherichia coli serotype with putative antiatherogenic effect [16].
Inhibition of human cytocrome P450 1A1, 1A2, 1B1, 2C8, and 3A4 by a series of flavonoid derivatives, including 5-hydroxyflavone, was studied and structure-function relationships were established. Knowing that these enzymes are involved in the activation and detoxification of endogenous chemicals and xenobiotics, the substances that inhibit them can influence the human health. 5-hydroxyflavone is more active than flavone in inhibiting P450 1A1 and P450 1B1, less active than flavones in inhibiting of P450 1A2 and it is weak in inhibiting P450 3A4 and P450 2C9 [17].
Due to the presence of a chelating 5-hydroxy-4-keto group, 5-hydroxyflavone can act as a bidentate ligand toward metal ions. The complexation process of 5-hydroxyflavone with some metal ions was investigated mainly in solution, using spectroscopic techniques. Investigation of the solvent effects on Al(III)-5-hydroxyflavone complexes revealed that while in pure methanol a stoichimetry of 1:1 was obtained [18], in methanol/water medium at pH 6 a species with 1:2 metal:ligand stoichiometry was formed [19]. Complexes with 1:1 stoichiometry were obtained in pure methanol also for Pb(II) [20] and Zn(II) [21]. Several complexes were obtained in solid state, for example those with Co(II), Ni(II), Cu(II), V(III), and Fe(III) [22], and VO(II) [23]. A solid mixed ligands complex of Ru(II) with 5-hydroxyflavone and dimethyl sulfoxide was also reported [24]. Applications of metal complexes are mainly based on their luminiscent properties. Some examples are the use of 5-hydroxyflavone-Al(III) complex as fluorescent fluoride ion probe [25] and of [Be(5Fla)2] (Fla = 5-hydroxyflavonate ion) as emitting material in organic light-emitting diodes [26]. Although so far, biological applications of metal complexes of 5-hydroxyflavone have not been reported, some recent studies that evidenced the hypoglycemic activity of 3-hydroxyflavone complexes with Zn(II) [27] and VO(II) [28] are encouraging for investigating the biological activity of complexes with 5-hydroxylated analogue.
The present work adds to the efforts to obtain new complexes of 5-hydroxyflavone with potential biological activity. Two new solid compounds of Mg(II) and Zn(II) with 5-hydroxyflavone were obtained. The composition and structure of complexes were investigated by elemental and thermal analysis, IR, 1H- and 13C-NMR spectroscopy and by mass spectra analysis. The nature of complexes was determined by measuring the conductance of DMSO solutions. Fluorescence properties of complexes were determined in solid state and in solution of various solvents, comparing to those of the free ligand.

2. Results and Discussion

The yellow compounds obtained according to the general reaction depicted in Scheme 1 are hardly soluble in water, their solubility being presented in Table 1. Table 2 shows the analytical and molar conductance data for the complexes. The low values of the molar conductance suggest the non-electrolytic nature of the complexes.
Molecules 18 07631 g009 550
Scheme 1. The reaction scheme for the synthesis of the complexes.
Scheme 1. The reaction scheme for the synthesis of the complexes.
Molecules 18 07631 g009
Table
Table 1. Solubility in different solvents of the parent ligand and its complexes.
Table 1. Solubility in different solvents of the parent ligand and its complexes.
CompoundDMSODMFAcCNCHCl3MeOHEtOH
HLsolublesolublesolublesolublesoluble at mild heatingsoluble at mild heating
[Mg(L)2(H2O)2]·H2O
(1)		solubleslightly solubleslightly solublesoluble at mild heatingslightly solubleslightly soluble
[Zn(L)2(H2O)2]·0.5H2O
(2)		solublesolublesolublesolubleslightly solubleslightly soluble
Table
Table 2. Analytical and molar conductance data for the complexes.
Table 2. Analytical and molar conductance data for the complexes.
CompoundMolecular formulaMolecular weight
(g mol−1)		Anal. found (calcd.) (%)Molar conductance ΛM (Ω−1 cm2 mol−1)
CHM
[Mg(L)2(H2O)2]·H2O
(1)		MgC30H24O9552.8264.95 (65.18)4.24 (4.38)4.10 (4.39)2
[Zn(L)2(H2O)2]·0.5H2O
(2)		ZnC30H23O8.5584.9061.40 (61.60)4.12 (3.96)10.92 (11.18)3.5

2.1. IR Spectra

IR spectra of the ligand and the complexes bring evidences of coordination of Mg(II) and Zn(II) ions to the 5-hydroxyflavone. The data are summarized in Table 3.
Table
Table 3. IR data (cm−1) for ligand and complexes.
Table 3. IR data (cm−1) for ligand and complexes.
Compoundν(O-H)ν(C=O)ν(C=C)ν(C-O) + δ(OH)ν(C-O-C)γw(H2O)
HL3,200–2,600 b, m1,654 s;
1,615 s		1,587 s1,357 m;
1,298 s		1,255 s-
[Mg(L)2(H2O)2]·H2O
(1)		3,600–2,600 b, m1,634 s1,583 s1,361 m;
1,297 w		1,251 s422 w
[Zn(L)2(H2O)2]·0.5H2O
(2)		3,600–2,600 b, m1,632 s1,580 s1,355 m;
1,297 w		1,250 s546 w
b: broad; m: medium; s: strong; w: weak.
In the high wavenumber region, the IR spectrum of 5-hydroxyflavone [Figure 1a] displays an intense broad band between 2,600 and 3,200 cm−1. This band is due to the strong intramolecular hydrogen bond involving the OH group, a characteristic feature of 5-hydroxylated chromones [29]. A sharp and intense band present in this region at 3,059 cm−1 corresponds to the stretching vibration ν(C-H). In the IR spectra of the complexes (Figure 2b,c) are found a broad band between 2,600 and 3,600 cm−1, assigned to the presence of water molecule in the structure of the complexes. Simultaneously, the ν(C-H) band is weakened and broadened by overlapping.
In the 1,550–1,750 cm−1 region, the ν(C=O) vibration of 5-hydroxyflavone generates two intense bands, placed at 1,654 and 1,615 cm−1. In the IR spectra of the complexes, a single strong band appears at around 1,633 cm−1. The displacement of ~20 cm−1 suggests the involvement of C=O group in coordination. The strong band characteristic for ν(C=C) which appears at 1,587 cm−1 in the IR spectrum of the ligand, is slightly shifted in the IR spectra of complexes, supporting that this bond is unaffected by coordination.
Between 1,000 and 1,500 cm−1 in the IR spectra bands associated with δ(OH) mode are presented, mixed with ν(C=O), ν(CC) and aromatic ring deformation. The strong band at 1,298 cm−1 from the coupled vibration ν(C-O) + δ(OH) [30] in the IR spectrum of 5-hydroxyflavone, appears very weakened in the IR spectra of complexes, that suggests its involvement in coordination in the deprotonated form. The ν(C-O-C) frequency is not shifted in the IR spectra of complexes comparing to the IR spectrum of the ligand, indicating that the ring oxygen is not involved in coordination.
The presence of the coordinated water in the structure of the complexes is indicated by the wagging frequencies at 422 for complex 1 and 546 cm−1 for complex 2, respectively [31].
Molecules 18 07631 g002 550
Figure 2. IR spectra of (a) 5-hydroxyflavone (HL). (b) [Mg(L)2(H2O)2]·H2O. (c) [Zn(L)2(H2O)2]·0.5H2O.
Figure 2. IR spectra of (a) 5-hydroxyflavone (HL). (b) [Mg(L)2(H2O)2]·H2O. (c) [Zn(L)2(H2O)2]·0.5H2O.
Molecules 18 07631 g002

2.2. UV-Vis Spectra

The diffuse reflectance electronic spectra of 5-hydroxyflavone exhibit two intensive absorption bands originated from π–π* transitions; the band centred at 396 nm (band I) is due to the transition localized within the B ring of cinnamoyl system, whereas the one centred at 280 nm (band II) may be assigned to transitions in the ring A of benzoyl system [32]. UV-Vis data for ligand and complexes are presented in Table 4.
The characteristic features in the spectra of complexs are the bathochromic shifts of the two bands of the ligand, due to the extension of the conjugated system with the complexation, as can be shown in Figure 3.
Table
Table 4. UV-Vis data for ligand and complexes.
Table 4. UV-Vis data for ligand and complexes.
Compoundλmax (nm)
Band IBand II
HL396280
[Mg(L)2(H2O)2]·H2O (1)406.5284
[Zn(L)2(H2O)2]·0.5H2O (2)403.5283
Molecules 18 07631 g003 550
Figure 3. Diffuse reflectance electronic spectra of (a) 5-hydroxyflavone (HL). (b) [Mg(L)2(H2O)2]·H2O. (c) [Zn(L)2(H2O)2]·0.5H2O.
Figure 3. Diffuse reflectance electronic spectra of (a) 5-hydroxyflavone (HL). (b) [Mg(L)2(H2O)2]·H2O. (c) [Zn(L)2(H2O)2]·0.5H2O.
Molecules 18 07631 g003

2.3. 1H-NMR and 13CNMR Spectra

Complexation of Mg(II) and Zn(II) to 5-hydroxyflavone was investigated by 1H- and 13C-NMR, using DMSO-d6 as solvent. The 1H chemical shifts for ligand and complexes are presented in Table 5 and were assigned based on the literature data [33], while the 13C- chemical shifts and their assignment [34] are done in Table 6. The main difference observed in the 1H-NMR spectrum of complexes is the absence of chemical signal of hydrogen from 5-OH phenolic group. Some of the other proton signals are shifted to lower frequencies relative to the free ligand or are broadened due to the fact that coordination increases the planarity of flavonoid molecule, therefore decreasing the mobility of the protons [35]. Slightly shifts of the chemical signals of carbon atoms belonging to the ring A and C are observed in the 13C-NMR spectra of complexes comparing to that of the free ligand. Instead, the signals of carbon atoms from the side ring B are practically unchanged, and these observation support the involvement of the chemical groups (phenolic OH and carbonyl C=O) on the rings A and C in complexation.
Table
Table 5. The 1H chemical shifts (ppm) for ligand and complexes.
Table 5. The 1H chemical shifts (ppm) for ligand and complexes.
Compoundδ of 1H (J, Hz)
H-3H-6H-7H-8H-3′H-4′H-5′H-2′H-6′OH
HL7.1 (s)6.8 (d, 8.3)7.6 (t, 8.1)7.2 (d, 8.1)7.6 (m)8.1 (dd, 7.8, 1.7)12.55 (s)
(1)6.8 (s)6.4(bd, 9.9)7.3 (t, 8.2)6.3 (bd, 8.2)7.6 (m)8.0 (dd, 8.0, 2.6)-
(2)7.0 (s)6.5 (d, 8.4)7.4 (t, 8.4)6.7 (d, 8.4)7.6 (m)8.1 (dd, 8.2, 1.7)-
Table
Table 6. The 13C chemical shifts (ppm) for ligand and complexes.
Table 6. The 13C chemical shifts (ppm) for ligand and complexes.
Compoundδ of 13C
C-2C-3C-4C-5C-6C-7C-8C-9C-10
HL164.1105.7183.2159.8111.0135.9107.5155.9110.1
(1)171.4106.2180.9161.1116.9134.996.8158.2113.4
(2)171.4106.0183.1162.1117.1135.499.7157.78112.2
Compoundδ of 13C
C-1′C-2′C-6′C-3′C-5′C-4′
HL130.53126.6129.2132.3
(1)131.11126.1129.2131.5
(2)130.72126.3129.2131.9

2.4. Mass Spectra

The direct injection of a acetonitrile solution of the complex into a ESI interface leads to the protonated molecular ion of the ligand [M+H]+ m/z = 239, obtained as main ion. However, if a solution of complex in acetonitrile/water with 0.1% ammonia 9/1 in the +ESI-MS spectrum, the protonated molecular ion of the complex could be also observed (Figure 4). The fragments resulted by collision with argon at a pressure of 1.5 mTorr comply the natural isotopic abundances of the metallic elements (Table 7). The data from the mass spectra suggest the 1:2 molar ratio metal ion:ligand.
Molecules 18 07631 g004 550
Figure 4. Mass spectra of (a) [Mg(L)2(H2O)2]·H2O. (b) [Zn(L)2(H2O)2]·0.5H2O.
Figure 4. Mass spectra of (a) [Mg(L)2(H2O)2]·H2O. (b) [Zn(L)2(H2O)2]·0.5H2O.
Molecules 18 07631 g004
Table
Table 7. Nominal m/z of the ions observed in the positive ion mode ESI-MS fingerprint of the complexes and the identified structure.
Table 7. Nominal m/z of the ions observed in the positive ion mode ESI-MS fingerprint of the complexes and the identified structure.
MLn speciesProtonated molecular ion [MLn+H]+ (m/z)Fragment at 5 eV and 1.5 mTorr argon (m/z)Type of fragmentFragmentation
24MgL249926124MgL+[24MgL+H-L]+
25MgL250026225MgL+[25MgL+H-L]+
26MgL250126326MgL+[26MgL+H-L]+
64ZnL253930164ZnL+[64ZnL+H-L]+
66ZnL254130366ZnL+[66ZnL+H-L]+
68ZnL254330568ZnL+[68ZnL+H-L]+

2.5. Thermal Behavior

The results regarding the thermal decomposition of complexes are described in the following section and are summarized in the Table 8.
Table
Table 8. Thermal degradation data (in synthetic air) for the complexes.
Table 8. Thermal degradation data (in synthetic air) for the complexes.
ComplexStepThermal effectTemperature range/°CΔmexp/%Δmcalc/%Chemical process
[Mg(L)2(H2O)2]·H2O
(1)		1.Endothermic52–903.03.3H2O loss
2.Endothermic120–1603.13.3H2O loss
3.Endothermic170–2063.23.3H2O loss
4.Exothermic408–90056.3 Partial oxidative degradation of organic part
Residue (MgO + organic residue)34.4
[Zn(L)2(H2O)2]·0.5H2O
(2)		1.Endothermic54–751.71.60.5 H2O loss
2.Endothermic85–1286.16.22H2O loss
3.Exothermic300–75078.578.3Oxidative degradation of organic part
Residue (ZnO)13.713.9

2.5.1. Thermal Decomposition of [Mg(L)2(H2O)2]·H2O

Thermal decomposition of complex 1 undergoes in four well-defined steps (Figure 5, Table 8). The first step corresponds to the loss of one water molecule. This endothermic step occurs at low temperature confirming the lattice nature of these. The next two endothermic events, could be associated with the release of the two coordinated water molecules.
The anhydrous compound is stable over a large temperature range (206–408 °C). The last exothermic decomposition step corresponds to oxidative degradation of the organic part. Tacking into accord the aspect of TG curve and the value of mass loss we can conclude that the oxidative degradation of organic part is not finished at 900 °C.
Molecules 18 07631 g005 550
Figure 5. TG and DTA curves of [Mg(L)2(H2O)2]·H2O.
Figure 5. TG and DTA curves of [Mg(L)2(H2O)2]·H2O.
Molecules 18 07631 g005

2.5.2. Thermal Decomposition of [Zn(L)2(H2O)2]·0.5H2O

The analysis of TG and DTA curves (Figure 6) allowed us to establish the final formula of this compound Thus, the first endothermic mass loss in the 50–90 °C temperature range corresponds to the lattice water molecules release. The two coordinated water molecules are loss in a single endothermic step. The anhydrous complex is stable up to 300 °C when it starts the oxidative degradation of organic ligand. This exothermic step is a complex one, consisting in at least three events, as shown both TG and DTA curves. The final residue is zinc oxide as powder X-ray diffraction indicated.
Molecules 18 07631 g006 550
Figure 6. TG and DTA curves of [Zn(L)2(H2O)2]·0.5H2O.
Figure 6. TG and DTA curves of [Zn(L)2(H2O)2]·0.5H2O.
Molecules 18 07631 g006

2.6. Fluorescent Properties

The fluorescence emission spectra (Figure 7 and Figure 8) were recorded at two excitation wavelengths, 400 and 429 nm, respectively, both for ligand and complexes. The results presented in Table 9 let us draw the following conclusions: (i) 5-hydroxyflavone itself exhibits a strong fluorescence; (ii) at the excitation wavelength of 400 nm (an absorption maximum in the UV-Vis spectra of ligand and complexes), the fluorescence emission intensity of complexes was markedly higher than that of the ligand at ~600 nm, and a new band of very high intensity appeared at ~545 nm in the fluorescence emission spectra of complexes; (iii) at the excitation wavelength of 429 nm (descending branch of the peak in the UV-Vis spectrum), the fluorescence emission intensity of complexes was a little bit higher than that of the ligand at 642 nm, the new band of very high intensity appeared at ~ 548 nm in the fluorescence spectra of complexes was still present, while the fluorescence emission intensity of complexes was smaller than that of the ligand at 458 nm. The enhancement in the fluorescence intensity of complexes may be related to the formation of a chelate ring via coordination of the metal ion, which increases the rigidity of the ligand structure and enhances the fluorescence quantum yield by reducing the probability of non-radiative dissipation process. The new emission band that appeared in the emission spectra of complexes could be appreciated as a evidence for the formation of a new bond, a metal-ligand bond.
Molecules 18 07631 g007 550
Figure 7. The emission spectra at λexc = 400 nm of (a) 5-hydroxyflavone (HL). (b) [Mg(L)2(H2O)2]·H2O. (c) [Zn(L)2(H2O)2]·0.5H2O.
Figure 7. The emission spectra at λexc = 400 nm of (a) 5-hydroxyflavone (HL). (b) [Mg(L)2(H2O)2]·H2O. (c) [Zn(L)2(H2O)2]·0.5H2O.
Molecules 18 07631 g007
Molecules 18 07631 g008 550
Figure 8. The emission spectra at λexc = 429 nm of (a) 5-hydroxyflavone (HL). (b) [Mg(L)2(H2O)2]·H2O. (c) [Zn(L)2(H2O)2]·0.5H2O.
Figure 8. The emission spectra at λexc = 429 nm of (a) 5-hydroxyflavone (HL). (b) [Mg(L)2(H2O)2]·H2O. (c) [Zn(L)2(H2O)2]·0.5H2O.
Molecules 18 07631 g008
Table
Table 9. Fluorescence data for ligand and complexes.
Table 9. Fluorescence data for ligand and complexes.
CompoundExcitation wavelength
λexc = 400 nm		Excitation wavelength
λexc = 429 nm
Emission wavelength
λem (nm)		Relative fluorescence
intensity (a.u.)		Emission wavelength
λem (nm)		Relative fluorescence
intensity (a.u.)
HL540
599		82.43
174.87		456
545
642		>1000
73.23
323.29
[Mg(L)2(H2O)2]·H2O
(1)		545
598		510.61
379.94		459
548
642		824.65
576.89
331.28
[Zn(L)2(H2O)2]·0.5H2O
(2)		548
598		536.83
410		458
548
642		727.71
627.11
346.30

3. Experimental

All reagents and solvents were of analytical reagent grade and were used without further purification. 5-hydroxyflavone, MgCl2·6H2O and Zn(OAc)2·2H2O were purchased from Aldrich Chemical Co., Schnelldorf, Germany.
Elemental analyses were performed using a Perkin Elmer PE 2400 analyser (for C, H, N, S) and a Shimadzu AA 6300 spectrometer (for magnesium and zinc). The conductivity was measured with a Consort C830 (Turnhout, Belgium) conductimeter with an SK10T platinum electrode embedded in glass (cell constant 1.0 cm−1). IR spectra were recorded using KBr pellets on a FT-IR VERTEX 70 (Bruker) spectrometer in the range 400–4,000 cm−1. Electronic spectra by diffuse reflectance technique, with magnesium oxide as reference sample, were recorded in the range 200–600 nm, on a Jasco V 650 spectrophotometer. Fluorescence spectra were recorded on a Jasco FP 6500 spectrofluorometer. For UV-Vis and fluorescence measurements, the solid sample was diluted with magnesium oxide (~1 mg of solid sample in 50 mg MgO). The 1H and 13C-NMR spectra were recorded on a NMR Varian Gemini 300 BB spectrometer working at 300 MHz for 1H and 75 MHz for 13C in in DMSO-d6. All chemical shifts are reported in δ (ppm) using TMS as the internal standard. Mass spectra were recorded by electrospray ionization tandem mass spectrometry (ESI-MS) technique. Solutions of 5 mg/mL in acetonitrile/water with 0.1% ammonia in 9/1 (v/v) ratio were injected directly into the electrospray interface of a 1200 L/MS/MS (Varian) mass spectrometer using a Prostar 240 SDM (Varian) pump. Molecular ions scanning range (m/z) was 150–1,500. The heating curves (TG, and DTA) were recorded using a Labsys 1200 SETARAM thermobalance with a sample weight between 10–14 mg over the temperature range of 20–900 °C and a heating rate of 10 °C min−1. The measurements were carried out in synthetic air atmosphere (flow rate 16.66 mL min−1), using alumina crucible. The X-ray powder diffraction patterns were collected on a DRON-3 diffractometer with a nickel filtered Cu Kα radiation (λ = 1.5418 Å) in 2θ range of 5–70°, a step width of 0.05° and an acquisition time of 2 s per step.
Synthesis of complexes was carried out following the general procedure described in [36]: an ethanolic solution (25 mL) of 5-hydroxyflavone (2 mmol, 0.476 g), deprotonated with TEA (2 mmol, 0.28 mL) was added to an ethanolic solution (5 mL) of metal salt (1 mmol, 0.2033 g MgCl2·6H2O or 0.2195 g Zn(OAc)2·2H2O). The reaction mixture was refluxed for 3 h. The yellow product formed was filtered off, washed several times with small amounts of ethanol, and dried in air (Yield: 92.8% [Mg(L)2(H2O)2]·H2O, 81% [Zn(L)2(H2O)2]·0.5H2O).

4. Conclusions

Primuletin (5-hydroxyflavone) forms in the selected working conditions ions two new complexes with Mg(II) and Zn(II), which have been characterized by elemental analyses, thermal analysis (TG, DTA), conductometric measurements and several spectroscopic techniques (IR, UV-Vis, 1H- and 13C-NMR, mass spectra). From the experimental data, the composition and structure, as well as the non-electrolytic nature of complexes have been established. The obtained complexes possess strong fluorescent properties when excited at 400 or 429 nm, valuable for future applications of these complexes. The biological potential of the complexes also worth explored.

Acknowledgments

The authors thank the Carol Davila University of Medicine and Pharmacy from Bucharest for the financial support.

Conflicts of Interest

The authors declare no conflict of interest.

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  • Sample Availability: Samples of the compounds 1 and 2 are available from the authors.

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MDPI and ACS Style

Uivarosi, V.; Badea, M.; Olar, R.; Drǎghici, C.; Bǎrbuceanu, Ş.F. Synthesis and Characterization of Some New Complexes of Magnesium (II) and Zinc (II) with the Natural Flavonoid Primuletin. Molecules 2013, 18, 7631-7645. https://doi.org/10.3390/molecules18077631

AMA Style

Uivarosi V, Badea M, Olar R, Drǎghici C, Bǎrbuceanu ŞF. Synthesis and Characterization of Some New Complexes of Magnesium (II) and Zinc (II) with the Natural Flavonoid Primuletin. Molecules. 2013; 18(7):7631-7645. https://doi.org/10.3390/molecules18077631

Chicago/Turabian Style

Uivarosi, Valentina, Mihaela Badea, Rodica Olar, Constantin Drǎghici, and Ştefania Felicia Bǎrbuceanu. 2013. "Synthesis and Characterization of Some New Complexes of Magnesium (II) and Zinc (II) with the Natural Flavonoid Primuletin" Molecules 18, no. 7: 7631-7645. https://doi.org/10.3390/molecules18077631

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