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Molecules 2013, 18(9), 10609-10628; doi:10.3390/molecules180910609

Effect of Salicylic Acid and Structurally Related Compounds in the Accumulation of Phytoalexins in Cotyledons of Common Bean (Phaseolus vulgaris L.) Cultivars

1
Grupo de Química de los Productos Naturales y los Alimentos, Escuela de Química, Facultad de Ciencias, Universidad Nacional de Colombia-Sede Medellín, Calle 59ª 63-020 Autopista Norte, P.O. Box 3840, Medellín, Colombia
2
Química Orgánica de Productos Naturales, Facultad de Ciencias Exactas y Naturales, Universidad de Antioquia, Calle 70 N° 52-21, P.O. Box 1226, Medellín, Colombia
*
Author to whom correspondence should be addressed.
Received: 26 July 2013 / Revised: 29 August 2013 / Accepted: 29 August 2013 / Published: 2 September 2013
(This article belongs to the Section Natural Products)
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Abstract

In the present work, isoflavonoid phytoalexin production in response to the application of salicylic acid in cotyledons of four common bean (Phaseolus vulgaris) cultivars (SA) was evaluated. The time-course and dose-response profiles of the induction process were established by quantifying the isoflavonoids by HPLC. Cotyledons of anthracnose-resistant cultivars induced by SA produced substantially higher phytoalexin contents as compared to the susceptible ones. In addition, maximum levels of phytoalexins (50–100 fold increases) were reached between 96 and 144 h, and when a concentration of SA from 3.62 to 14.50 mM was used. The observations also indicate that there was a relatively good correlation between the phytoalexin contents and the inhibitory effect against C. lindemuthianum; the higher antifungal activity was observed during the first 48 hours for extracts from cotyledons treated with SA at 1.45 and 3.62 mM, and between 96 and 144 h after induction. Finally, compounds structurally related to SA (dihydro-quinazolinones and some imines) showed a strong elicitor effect. Moreover, induced extracts from cotyledons treated with these potential elicitors, besides the properly elicitors, displayed a weak to moderated antifungal activity. These compounds may be considered good candidates for developing of new phytoprotectants. Furthermore, phytoalexin-eliciting substances may contribute for selecting disease resistant cultivars.
Keywords: cultivars; elicitor; phaseollin; dose-response profiles; Colletotrichum lindemuthianum; antifungal activity; dihydro-quinazolinones cultivars; elicitor; phaseollin; dose-response profiles; Colletotrichum lindemuthianum; antifungal activity; dihydro-quinazolinones
This is an open access article distributed under the Creative Commons Attribution License (CC BY 3.0).

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MDPI and ACS Style

Durango, D.; Pulgarin, N.; Echeverri, F.; Escobar, G.; Quiñones, W. Effect of Salicylic Acid and Structurally Related Compounds in the Accumulation of Phytoalexins in Cotyledons of Common Bean (Phaseolus vulgaris L.) Cultivars. Molecules 2013, 18, 10609-10628.

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