Next Article in Journal
The Use of Liposomes and Nanoparticles as Drug Delivery Systems to Improve Cancer Treatment in Dogs and Cats
Previous Article in Journal
Improved Performance of Magnetic Cross-Linked Lipase Aggregates by Interfacial Activation: A Robust and Magnetically Recyclable Biocatalyst for Transesterification of Jatropha Oil
Previous Article in Special Issue
Biodegradable Chitosan Decreases the Immune Response to Trichinella spiralis in Mice
Article Menu

Export Article

Open AccessArticle
Molecules 2017, 22(12), 1745; doi:10.3390/molecules22121745

Response of Ustilago maydis against the Stress Caused by Three Polycationic Chitin Derivatives

1
Instituto Politécnico Nacional, Escuela Nacional de Ciencias Biológicas, Departamento de Microbiología, Prolongación de Carpio y Plan de Ayala S/N, Col. Sto. Tomas, Del, Miguel Hidalgo, CP 11340 Ciudad de México, Mexico
2
Universidad Nacional Autónoma de México, Instituto de Fisiología Celular, Circuito exterior S/N, Ciudad Universitaria, CP 04510 Ciudad de México, Mexico
3
Universidad Nacional Autónoma de México, Facultad de Medicina, Departamento de Bioquímica, Circuito exterior S/N, Ciudad Universitaria, CP 04510 Ciudad de México, Mexico
*
Author to whom correspondence should be addressed.
Received: 10 August 2017 / Revised: 9 October 2017 / Accepted: 13 October 2017 / Published: 7 December 2017
View Full-Text   |   Download PDF [4218 KB, uploaded 7 December 2017]   |  

Abstract

Chitosan is a stressing molecule that affects the cells walls and plasma membrane of fungi. For chitosan derivatives, the action mode is not clear. In this work, we used the yeast Ustilago maydis to study the effects of these molecules on the plasma membrane, focusing on physiologic and stress responses to chitosan (CH), oligochitosan (OCH), and glycol-chitosan (GCH). Yeasts were cultured with each of these molecules at 1 mg·mL−1 in minimal medium. To compare plasma membrane damage, cells were cultivated in isosmolar medium. Membrane potential (Δψ) as well as oxidative stress were measured. Changes in the total plasma membrane phospholipid and protein profiles were analyzed using standard methods, and fluorescence-stained mitochondria were observed. High osmolarity did not protect against CH inhibition and neither affected membrane potential. The OCH did produce higher oxidative stress. The effects of these molecules were evidenced by modifications in the plasma membrane protein profile. Also, mitochondrial damage was evident for CH and OCH, while GCH resulted in thicker cells with fewer mitochondria and higher glycogen accumulation. View Full-Text
Keywords: chitosan; oligochitosan; glycol-chitosan; Ustilago maydis; stress response chitosan; oligochitosan; glycol-chitosan; Ustilago maydis; stress response
Figures

Figure 1

This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. (CC BY 4.0).

Scifeed alert for new publications

Never miss any articles matching your research from any publisher
  • Get alerts for new papers matching your research
  • Find out the new papers from selected authors
  • Updated daily for 49'000+ journals and 6000+ publishers
  • Define your Scifeed now

SciFeed Share & Cite This Article

MDPI and ACS Style

Olicón-Hernández, D.R.; Uribe-Alvarez, C.; Uribe-Carvajal, S.; Pardo, J.P.; Guerra-Sánchez, G. Response of Ustilago maydis against the Stress Caused by Three Polycationic Chitin Derivatives. Molecules 2017, 22, 1745.

Show more citation formats Show less citations formats

Note that from the first issue of 2016, MDPI journals use article numbers instead of page numbers. See further details here.

Related Articles

Article Metrics

Article Access Statistics

1

Comments

[Return to top]

Molecules EISSN 1420-3049 Published by MDPI AG, Basel, Switzerland RSS E-Mail Table of Contents Alert
Back to Top