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Molecules 2017, 22(12), 1745; doi:10.3390/molecules22121745

Response of Ustilago maydis against the Stress Caused by Three Polycationic Chitin Derivatives

Instituto Politécnico Nacional, Escuela Nacional de Ciencias Biológicas, Departamento de Microbiología, Prolongación de Carpio y Plan de Ayala S/N, Col. Sto. Tomas, Del, Miguel Hidalgo, CP 11340 Ciudad de México, Mexico
Universidad Nacional Autónoma de México, Instituto de Fisiología Celular, Circuito exterior S/N, Ciudad Universitaria, CP 04510 Ciudad de México, Mexico
Universidad Nacional Autónoma de México, Facultad de Medicina, Departamento de Bioquímica, Circuito exterior S/N, Ciudad Universitaria, CP 04510 Ciudad de México, Mexico
Author to whom correspondence should be addressed.
Received: 10 August 2017 / Revised: 9 October 2017 / Accepted: 13 October 2017 / Published: 7 December 2017
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Chitosan is a stressing molecule that affects the cells walls and plasma membrane of fungi. For chitosan derivatives, the action mode is not clear. In this work, we used the yeast Ustilago maydis to study the effects of these molecules on the plasma membrane, focusing on physiologic and stress responses to chitosan (CH), oligochitosan (OCH), and glycol-chitosan (GCH). Yeasts were cultured with each of these molecules at 1 mg·mL−1 in minimal medium. To compare plasma membrane damage, cells were cultivated in isosmolar medium. Membrane potential (Δψ) as well as oxidative stress were measured. Changes in the total plasma membrane phospholipid and protein profiles were analyzed using standard methods, and fluorescence-stained mitochondria were observed. High osmolarity did not protect against CH inhibition and neither affected membrane potential. The OCH did produce higher oxidative stress. The effects of these molecules were evidenced by modifications in the plasma membrane protein profile. Also, mitochondrial damage was evident for CH and OCH, while GCH resulted in thicker cells with fewer mitochondria and higher glycogen accumulation. View Full-Text
Keywords: chitosan; oligochitosan; glycol-chitosan; Ustilago maydis; stress response chitosan; oligochitosan; glycol-chitosan; Ustilago maydis; stress response

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This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. (CC BY 4.0).

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Olicón-Hernández, D.R.; Uribe-Alvarez, C.; Uribe-Carvajal, S.; Pardo, J.P.; Guerra-Sánchez, G. Response of Ustilago maydis against the Stress Caused by Three Polycationic Chitin Derivatives. Molecules 2017, 22, 1745.

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