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Article

Synthesis and Antitumor Activities of Chiral Dipeptide Thioureas Containing an Alpha-Aminophosphonate Moiety

1
Engineering Research Center for the Development and Application of Ethnic Medicine and TCM (Ministry of Education), Guizhou Medical University, Guiyang 550004, Guizhou, China
2
Guizhou Provincial Key Laboratory of Pharmaceutics, Guizhou Medical University, Guiyang 550004, Guizhou, China
3
School of Pharmacy, Guizhou Medical University, Guiyang 550004, Guizhou, China
4
National Engineering Research Center of Miao’s Medicines, Guizhou Medical University, Guiyang 550004, Guizhou, China
5
School of Pharmacy, Zunyi Medical College, Zunyi 563000, Guizhou, China
6
School of Basic Medicine, Guizhou Medical University, Guiyang 550004, Guizhou, China
*
Authors to whom correspondence should be addressed.
Molecules 2017, 22(2), 238; https://doi.org/10.3390/molecules22020238
Submission received: 18 December 2016 / Revised: 26 January 2017 / Accepted: 30 January 2017 / Published: 16 February 2017

Abstract

:
Thiourea derivatives demonstrate potent cytotoxic activity against various leukemias and many tumor cell lines. In our previous study, the combination of thiourea and phosphonate has been proven as an effective strategy for developing antitumor agents. Herein, we synthesized and evaluated a series of novel chiral dipeptide thioureas containing an α-aminophosphonate moiety as antitumor agents. Finally, we developed novel dipeptide thioureas 11d and 11f that showed comparable inhibition with that of Cisplatin against BGC-823 and A-549 cells, respectively.

Graphical Abstract

1. Introduction

It is well-known that thiourea derivatives demonstrate potent cytotoxic activity against various leukemias and many tumor cell lines [1,2], and it is believed that this is due to their good inhibitory activity against protein tyrosine kinases (PTKs) [3,4,5,6], receptor tyrosine kinases (RTKs) [7], DNA topoisomerase [8,9,10], sirtuins [11], carbonic anhydrase [12], vanilloid receptor [13], a family of G protein-coupled receptors (sst1-5) [14], etc. In the past few decades, many thiourea derivatives have been reported on regarding their synthesis, evaluation as anticancer agents, structure-activity relationships, and mechanism of action [15,16,17,18]. For example, the derivatives show enhanced anticancer activities when thioureas are conjugated with amino acids [2]. We also combined amino acid and α-aminophosphonate to develop novel thiourea derivatives, called pseudo-peptide thioureas [19], which turned out to be another type of potential anticancer agents. Other functionalized thioureas were structurally incorporated with other scaffolds [2], such as benzensulfonamide [20], chlorocolchicine [21], podophyllotoxin [22], pyrazole [23], benzimidazole [24], benzothiazole [25], etc.
On the other hand, peptide conjugated derivatives have attracted much attention in recent years because of their broad biological activity, biocompatibility, and the possibility of introducing structural diversity in them, which became an effective strategy to obtain novel chemical entities when small bioactive motifs are conjugated with peptides [26]. Over the years, the conjugated molecules, as novel chemotherapeutics, have made significant progress due to the accessibility of combining the structural features of two or more small bioactive motifs to achieve novel molecules with enhanced bioactivities.
Based on our previous study [19], we would like to further introduce one more amino acid to the pseudo-peptide thiourea containing an α-aminophosphonate moiety. Herein, we describe the synthesis of a series of novel chiral dipeptide thioureas containing α-aminophosphonate moieties. The structures of the synthesized compounds were characterized by IR (Infrared spectrum), 1H-NMR (Nuclear Magnetic Resonance), 13C-NMR, 31P-NMR, 19F-NMR, and elemental analysis studies. The anticancer potency of all of these novel thioureas was examined in the human gastric cancer cell line BGC-823 and the human non-small cell lung cancer cell line A-549. To the best of our knowledge, this is the first report on the synthesis and antitumor activity of these dipeptide thioureas containing α-aminophosphonate moieties. These novel thioureas may provide promising lead compounds for treating human cancer.

2. Results

According to our pseudo-peptide thioureas containing α-aminophosphonate moieties described previously [19], we planned to introduce glycine or L-proline to obtain the novel thioureas 10 and 11 (Figure 1), respectively. The synthesis is outlined in Figure 1. Substituted benzylamine 1 was coupled with commercially available N-Boc protected glycine 2 or N-Boc protected L-proline 3 in the presence of O-benzotriazol-1-yl-N,N,N’,N’-tetramethyluronium hexafluorophosphate (HBTU). The Boc groups of the resulting compounds 4 and 5 were removed by trifluoroacetic acid (TFA) in CH2Cl2. Similarly, the dipeptides 7 and 8 were achieved by coupling with amino acid 6 after the deprotection. The key intermediate, O,O’-dialkylisothiocyanato (phenyl) methylphosphonate 9, was prepared as described previously [19]. Finally, these novel thioureas 10 and 11 were obtained by nucleophilic addition of α-phosphonate isothiocyanate to the intermediate 9. The structures, yields, and melting points of all these novel thioureas 10 and 11 are displayed in Table 1. All these novel thioureas were obtained in modest to excellent yields (from 53.9% to 98.4%).
With these novel thioureas in hands, we then selected two human cancer cell lines, BGC-823 and A-549, which are derived from stomach cancer and non-small cell lung cancer (NSCLC), respectively, and they were used to evaluate the anti-proliferation activities of thioureas 10 and 11 with a series concentration of 500 to 3.9 µM by comparison with a commercial anticancer drug, Cisplatin. As shown in Table 2, by the conventional 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay, thioureas 10 and 11 both demonstrated the capability of inhibiting the proliferation of BGC-823 and A549 cells with IC50 values in the range of 20.9 to 103.6 µM and 19.2 to 112.5 µM, respectively. Overall, thiourea 11 with the incorporation of L-proline demonstrated better anti-cancer activities in these two cancer cell lines than that of thiourea 10 with the incorporation of glycine (10gl vs. 11af, Table 2). Among the O,O′-Dialkylphosphonates derived from the ethyl, n-propyl, or iso-propyl groups of these compounds, different substituted phosphonate esters demonstrated no significant effect on the antitumor activities (Table 2). However, in some cases where R2 = n-propyl, the IC50 values were higher with respect to the other cases (for example: 10b vs. 10a,c; 10g vs. 10h,i for BGC-823, Table 2), which suggested that the n-propyl group may have a negative effect. Phenylalanine containing thiourea 10 or 11 (R1 = L-Ph) demonstrated better antitumor activity than that of phenylglycine containing thiourea 10 or 11 (R1 = L-Bn) (10gl vs. 10af, Table 2). Additionally, the para-fluorinated benzyl thioureas elicited superior antitumor activity to non-substituted benzyl thioureas (11df vs. 11ac; 10df vs. 10ac; 10jl vs. 10gI; except for 11d vs. 11a for A549, Table 2). Notably, thiourea 11d showed comparable inhibition with that of Cisplatin against BGC-823 cells (IC50 = 20.9 µM vs. 15.1 µM), while thiourea 11f showed the highest inhibitory activity, close to that of Cisplatin, against A-549 cells (IC50 = 19.2 µM vs. 17.6 µM).

3. Materials and Methods

3.1. Materials

Reagents were obtained from Aldrich or Acros (Waltham, MA, USA) in the highest purity available and were used as supplied. The melting points of the products were determined on a XT-4 binocular microscope (Beijing Tech Instrument Co., Beijing, China) and were not corrected. The IR spectra were recorded on a Bruker VECTOR22 spectrometer in KBr disks (Billerica, MA, USA). 1H- and 13C-NMR spectra were recorded on a JEOL-300 NMR spectrometer (Otemachi, Chiyoda, Tokyo, Japan) at room temperature using tetramethylsilane (TMS) as an internal standard for 1H- and 13C-NMR while using H3PO4 (85%) and CFCl3 as an external reference for 31P- and 19F-NMR, respectively. The reported 13C chemical shifts are those present in the spectrum, and the 31P-13C coupling (or 19F-13C coupling) has not been considered. Elemental analysis was performed on an Elementar Vario-III CHN analyzer (Elementar, Frankfurt, Germany). UV spectra were recorded on a VARIAN Cary-50 spectrometer (Santa Clara, CA, USA) using a cell path length of 1 cm. BIO-RAD, Model 680 Microplate Reader (Hercules, CA, USA) was used to record those absorptions after MTT assay. The reagents were all of analytical grade or were chemically pure. Analytical thin layer chromatography (TLC) was performed on silica gel GF254.

3.2. Synthesis

A solution of O,O′-dialkylisothiocyanato (phenyl) methylphosphonate 9 [19] (1 mmol) in tetrahydrofuran (10 mL) was stirred, followed by drop wise addition of the intermediate 7 or 8 (1.1 mmol). The reaction mixture was stirred for 1 h at 23 °C, the solvent was removed by evaporation, and the crude product was purified by flash chromatography on silica using a mixture of petroleum ether and ethyl acetate as the eluent to yield the compounds 10al and 11af in 53.9%–98.4% yields. All final compounds were confirmed by 1H-NMR, 13C-NMR, IR, and elemental analysis.
10a: C29H35N4O5PS, white solid, yield 91.6%, m.p. 179–180 °C, [α] D 20 = +57 (c = 0.12, CHCl3) IR ν: 3291, 3084, 3065, 3032, 2982, 2928, 2908, 1652, 1535, 1497, 1453, 1352, 1336, 1226, 1050, 1026 cm−1; 1H-NMR (300 MHz, CDCl3) δ: 8.61 (s, 1H, NH), 8.02 (s, 1H, NH), 7.93 (s, 1H, NH), 7.45–7.09 (m, 15H, ArH), 6.51 (dd, J = 22.3, 9.2 Hz, 1H, NH), 6.38 (dd, J = 22.2, 9.8 Hz, 1H, NCH-P), 4.93 (s, 1H, NCH), 4.18 (dd, J = 26.5, 4.8Hz, 4H, 2OCH2), 4.03–3.83 (m, 2H, NCH2-Ar), 3.70–3.52 (m, 2H, NCH2), 1.09 (t, J = 7.0 Hz, 3H, CH3), 1.03–0.92 (m, 3H, CH3) ; 13C-NMR (75 MHz, CDCl3) δ: 183.7, 171.5, 168.5, 137.9, 137.4, 135.1, 128.9, 128.5, 128.4, 127.6, 127.3, 63.4, 62.6, 61.9, 54.0, 43.4, 43.1, 16.1; 31P-NMR δ: 21.2 ppm; Anal. Calcd. (Analysis Calculated) for C29H35N4O5PS: C 60.17, H 5.72, N 9.99; Found: C 59.78, H 6.05, N 9.62.
10b: C31H39N4O5PS, white solid, yield 81.5%, m.p. 155–157 °C, [α] D 20 = +44 (c = 0.10, CHCl3) IR ν: 3295, 3083, 3064, 3031, 2969, 2935, 1654, 1541, 1497, 1454, 1355, 1227, 1204, 1058, 1012 cm−1; 1H-NMR (300 MHz, CDCl3) δ: 8.10 (d, J = 96.3 Hz, 2H, 2NH), 7.48–6.94 (m, 15H, ArH), 6.33 (s, 1H, NCH-P), 6.21 (s, 1H, NCH), 4.06 (dt, J = 82.5, 52.6 Hz, 2H, NCH2-Ar), 3.52 (s, 2H, NCH2), 3.31 (s, 2H, OCH2), 2.93 (s, 2H, OCH2), 1.57 (s, 4H, 2CH2), 1.40–1.35 (m, 2H, 2NH), 0.87 (d, J = 7.4 Hz, 6H, 2CH3); 13C-NMR (75 MHz, CDCl3) δ: 183.5, 171.5, 169.0, 138.0, 135.2, 129.0, 127.5, 127.1, 69.4, 69.0, 62.6, 53.8, 43.2, 42.1, 23.8, 23.6, 10.1, 9.9; 31P-NMR δ: 21.3 ppm; Anal. Calcd. for C31H39N4O5PS: C 60.60, H 6.61, N 8.79; Found: C 60.97, H 6.44, N 9.17.
10c: C31H39N4O5PS, white solid, yield 78.6%, m.p. 203–204 °C, [α] D 20 = +69 (c = 0.14, CHCl3) IR ν: 3323, 3269, 3089, 3031, 2980, 2932, 1678, 1639, 1537, 1512, 1453, 1224, 1002 cm−1; 1H-NMR (300 MHz, CDCl3) δ: 8.26 (s, 2H, 2NH), 8.03 (s, 2H, 2NH), 7.58–7.11 (m, 15H), 6.32 (d, J = 9.0 Hz, 1H, NCH-P), 4.73 (s, 1H, NCH), 4.49 (s, 2H, 2OCH), 4.31 (t, J = 30.3Hz, 2H, NCH2-Ar), 4.11 (d, J = 5.7 Hz, 2H, NCH2), 1.21–1.00 (m, 12H, 4CH3); 13C-NMR (75 MHz, CDCl3) δ: 171.4, 168.5, 138.0, 135.5, 129.0, 127.8, 127.3, 127.0, 72.7, 72.4, 61.8, 53.7, 43.4, 43.1, 24.4, 23.9, 23.6, 23.2; 31P-NMR δ: 19.4 ppm; Anal. Calcd. for C31H39N4O5PS: C 61.31, H 6.18, N 9.43; Found: C 60.97, H 6.44, N 9.17.
10d: C29H34FN4O5PS, white solid, yield 93.3%, m.p. 74–75 °C, [α] D 20 = +98 (c = 0.11, CHCl3) IR ν: 3294, 3065, 3033, 2987, 2931, 2910, 1673, 1539, 1510, 1221, 1204, 1049, 1024, 977 cm−1; 1H-NMR (300 MHz, CDCl3) δ: 8.68 (s, 1H, NH), 8.45 (s, 1H, NH), 7.48–7.06 (m, 12H, ArH), 7.00 (s, 1H, ArH), 6.88 (s, 1H, ArH), 6.79 (s, 1H, NCH-P), 5.26 (s, 1H, NCH), 4.33 (m, 2H, NCH2-Ar), 3.78 (m, 2H, NCH2), 3.59 (d, J = 6.8 Hz, 2H, OCH2), 3.39 (s, 2H, OCH2), 2.35 (s, 1H, NH), 2.01 (s, 1H, NH), 1.22 (t, J = 6.1 Hz, 3H, CH3), 0.98–0.85 (m, 3H, CH3); 13C-NMR (75 MHz, CDCl3) δ: 183.7, 171.5, 169.0, 160.8, 137.1, 135.0, 133.8, 129.6, 129.1, 128.45, 128.0, 127.5, 127.3, 115.4, 115.1, 63.5, 62.8, 62.2, 53.8, 42.7, 42.2, 14.3, 12.0; 31P-NMR δ: 21.1; 19F-NMR δ: −115.7 ppm; Anal. Calcd. for C29H34FN4O5PS: C 58.24, H 5.45, N 9.51; Found: C 57.99, H 5.71, N 9.33.
10e: C31H38FN4O5PS, white solid, yield 75.5%, m.p. 133–134 °C, [α] D 20 = +113 (c = 0.13, CHCl3) IR ν: 3290, 3066, 3036, 2972, 2938, 1673, 1540, 1511, 1454, 1352, 1337, 1223, 1062, 1010 cm−1; 1H-NMR (300 MHz, CDCl3) δ: 8.00 (s, 1H, NH), 7.82 (s, 1H, NH), 7.45–7.10 (m, 13H, ArH), 7.05 (s, 1H, ArH), 6.87 (d, J = 29.2 Hz, 2H, 2NH), 6.35 (s, 2H, NCH-P+ NCH), 4.42–3.95 (m, 2H, NCH2-Ar), 3.80 (s, 2H, NCH2), 3.64 (s, 2H, OCH2), 3.43 (d, J = 73.9 Hz, 2H, OCH2), 1.62 (s, 2H, CH2), 1.43 (d, J = 12.9 Hz, 2H, CH2), 0.79 (dt, J = 16.7, 7.4 Hz, 6H, 2CH3); 13C-NMR (75 MHz, CDCl3) δ: 183.8, 171.5, 168.6, 163.2, 137.1, 135.1, 129.5, 128.9, 128.7, 128.2, 128.1, 127.3, 115.4, 115.1, 69.5, 69.2, 62.7, 53.8, 42.1, 23.9, 23.6, 10.2, 10.0; 31P-NMR δ: 21.4; 19F-NMR δ: −115.8 ppm; Anal. Calcd. for C31H38FN4O5PS: C 59.36, H 5.87, N 9.12; Found: C 59.22, H 6.09, N 8.91.
10f: C31H38FN4O5PS, white solid, yield 77.8%, m.p. 176–177 °C, [α] D 20 = +85 (c = 0.14, CHCl3) IR ν: 3321, 3289, 3089, 3067, 2981, 2932, 1641, 1533, 1511, 1222, 999 cm−1; 1H-NMR (300 MHz, CDCl3) δ: 8.98 (s, 1H, NH), 8.45 (s, 1H, NH), 8.32 (d, J = 33.4 Hz, 2H, NH), 7.48–7.06 (m, 14H, ArH), 6.79 (s, 1H, NCH-P), 5.26 (s, 1H, NCH), 4.33 (m, 2H, NCH2Ar), 3.78 (m, 2H, NCH2), 3.59 (d, J = 6.8 Hz, 2H, OCH), 3.39 (s, 2H, OCH), 1.50–0.98 (m, 12H, 4CH3); 13C-NMR (75 MHz, CDCl3) δ: 183.6, 171.6, 168.6, 160.7, 138.2, 135.3, 133.9, 130.3, 129.5, 128.7, 127.9, 126.2, 115.9, 114.5, 73.4, 62.3, 55.3, 55.1, 43.1, 42.7, 24.7, 24.2, 23.6, 23.2; 31P-NMR δ: 19.5; 19F-NMR δ: −115.6 ppm; Anal. Calcd. for C31H38FN4O5PS: C 59.36, H 6.30, N 9.12; Found: C 59.22, H 6.09, N 8.91.
10g: C30H37N4O5PS, white solid, yield 66.8%, m.p. 167–168 °C, [α] D 20 = +47 (c = 0.10, CHCl3) IR ν: 3292, 3084, 3064, 3029, 2983, 2927, 1653, 1539, 1454, 1225, 1049, 1024, 976 cm−1; 1H-NMR (300 MHz, CDCl3) δ: 8.35 (s, 2H, 2NH), 8.04 (s, 2H, 2NH), 7.52–7.09 (m, 15H, ArH), 6.51 (s, 1H, NCH-P), 5.39 (d, J = 35.5 Hz, 1H, NCH), 4.39 (s, 2H, NCH-Ar), 4.10 (dd, J = 38.8, 23.9 Hz, 4H, 2OCH2), 3.77 (s, 1H, NCH), 3.57 (s, 1H, NCH), 2.99 (s, 1H, CHAr), 2.92 (s, 1H, CHAr), 1.06 (t, J = 7.0 Hz, 6H, 2CH3); 13C-NMR (75 MHz, CDCl3) δ: 183.2, 172.1, 168.2, 138.1, 136.5, 135.5, 129.4, 128.6, 128.1, 127.8, 127.3, 127.0, 63.7, 63.4, 59.8, 53.6, 43.4, 36.6, 16.2; 31P-NMR δ: 21.3 ppm; Anal. Calcd. for C30H37N4O5PS: C 60.61, H 6.07, N 9.86; Found: C 60.39, H 6.25, N 9.39.
10h: C32H41N4O5PS, white solid, yield 71.4%, m.p. 148–149 °C, [α] D 20 = +54 (c = 0.12, CHCl3) IR ν: 3294, 3084, 3064, 3031, 2969, 2935, 1654, 1542, 1497, 1454, 1355, 1336, 1227, 1056, 1007 cm−1; 1H-NMR (300 MHz, CDCl3) δ: 8.32 (d, J = 44.4 Hz, 2H, 2NH), 7.99 (s, 1H, NH), 7.51–7.03 (m, 15H, ArH), 6.92 (s, 1H, NH), 6.49 (s, 1H, NCH-P), 5.29 (s, 1H, NCH), 4.47 (m, 2H, NCH2-Ar), 4.38 (m, 2H, NCH2), 3.82 (m, 2H, OCH2), 3.62 (d, J = 30.8 Hz, 2H, OCH2), 3.19–2.85 (m, 2H, CH2Ar), 1.61 (d, J = 11.1 Hz, 2H, CH2), 1.49–1.29 (m, 2H, CH2), 0.98–0.79 (m, 3H, CH3), 0.71 (t, J = 7.3 Hz, 3H, CH3); 13C-NMR (75 MHz, CDCl3) δ: 183.2, 172.0, 168.1, 138.1, 136.5, 135.6, 129.4, 128.7, 128.2, 127.8, 127.5, 127.1, 69.2, 68.7, 53.8, 43.4, 39.5, 23.7, 10.3, 10.1; 31P-NMR δ: 21.4 ppm; Anal. Calcd. for C32H41N4O5PS: C 61.15, H 6.87, N 9.04; Found: C 61.52, H 6.61, N 8.97.
10i: C32H41N4O5PS, white solid, yield 70.4%, m.p. 72–73 °C, [α] D 20 = +76 (c = 0.11, CHCl3) IR ν: 3296, 3085, 3064, 3031, 2981, 2932, 1651, 1536, 1225, 998 cm−1; 1H-NMR (300 MHz, CDCl3) δ: 8.35 (s, 2H, 2NH), 8.04 (s, 2H, 2NH), 7.38–6.90 (m, 15H, ArH), 6.51 (s, 1H, NCH-P), 5.39 (d, J = 35.5 Hz, 1H, NCH), 4.39 (s, 2H, NCH-Ar), 3.99 (d, J = 4.4 Hz, 2H, 2OCH), 3.77 (s, 1H, NCH), 3.57 (s, 1H, NCH), 2.99 (s, 1H, CHAr), 2.92 (s, 1H, CHAr), 1.06 (t, J = 7.0Hz, 6H, 2CH3) ; 13C-NMR (75 MHz, CDCl3) δ: 183.1, 171.8, 171.2, 137.9, 136.3, 135.7, 129.2, 128.6, 128.3, 128.1, 127.5, 127.1, 126.9, 72.7, 72.3, 60.4, 53.6, 43.3, 42.6, 39.2, 24.1, 23.7, 23.0; 31P-NMR δ: 19.6 ppm; Anal. Calcd. for C32H41N4O5PS: C 61.91, H 6.28, N 9.35; Found: C 61.52, H 6.61, N 8.97.
10j: C30H36FN4O5PS, white solid, yield 98.7%, m.p. 75–76 °C, [α] D 20 = +107 (c = 0.12, CHCl3) IR ν: 3291, 3077, 3065, 3032, 2987, 2931, 1673, 1615, 1542, 1511, 1221, 1049, 1025, 976 cm−1; 1H-NMR (300 MHz, CDCl3) δ: 8.24 (s, 1H, NH), 7.97 (s, 1H, NH), 7.47–6.77 (m, 14H), 6.44 (s, 1H, NCH-P), 5.28 (s, 1H, NCH), 4.36–3.84 (m, 4H, NCH2Ar+ NCH2), 3.60 (d, J = 56.9 Hz, 4H, 2OCH2), 3.03 (d, J = 37.2 Hz, 2H, CH2-Ar), 2.19–1.86 (m, 2H, 2NH), 1.24 (dd, J = 13.2, 6.2 Hz, 3H, CH3), 1.01 (d, J = 1. 8 Hz, 3H, CH3) ; 13C-NMR (75 MHz, CDCl3) δ: 183.4, 171.9, 171.4, 163.3, 136.4, 135.5, 134.0, 129.8, 128.9, 128.5, 128.1, 127.1, 115.4, 115.2, 60.6, 60.1, 53.7, 42.8, 42.1, 39.1, 17.6, 14.4; 31P-NMR δ: 21.1; 19F-NMR δ: −115.5 ppm; Anal. Calcd. for C30H36FN4O5 PS: C 58.35, H 6.13, N 8.87; Found: C 58.62, H 5.90, N 9.11.
10k: C32H40FN4O5PS, white solid, yield 94.3%, m.p. 95–96 °C, [α] D 20 = +131 (c = 0.13, CHCl3) IR ν: 3294, 3066, 3032, 2970, 2935, 1653, 1540, 1511, 1454, 1353, 1338, 1223, 1062, 1009 cm−1; 1H-NMR (300 MHz, CDCl3) δ: 8.27 (s, 2H, 2NH), 7.82 (s, 2H, 2NH), 7.56–6.77 (m, 14H, ArH), 6.49 (s, 1H, NCH-P), 5.30 (s, 1H, NCH), 4.39 (s, 2H, NCH2-Ar), 3.89–3.78 (m, 4H, 2OCH2), 3.51 (d, J = 7.2 Hz, 2H, NCH2), 3.02 (d, J = 54.6 Hz, 2H, CH2-Ar), 1.62 (d, J = 10.4 Hz, 2H, CH2), 1.50–1.41 (m, 2H, CH2), 1.00–0.83 (m, 3H, CH3), 0.73 (t, J = 6.2 Hz, 2H, CH3); 13C-NMR (75 MHz, CDCl3) δ: 183.3, 171.9, 168.2, 160.9, 136.4, 135.6, 133.9, 129.7, 129.3, 128.8, 128.2, 127.2, 115.5, 115.2, 69.3, 69.2, 60.1, 53.8, 42.7, 39.4, 24.0, 23.8, 10.1; 31P-NMR δ: 21.4; 19F-NMR δ: −115.6; ppm; Anal. Calcd. for C32H40FN4O5PS: C 59.59, H 6.01, N 8.94; Found: C 59.80, H 6.27, N 8.72.
10l: C32H40FN4O5PS, white solid, yield 95.0%, m.p. 62–63 °C, [α] D 20 = +86 (c = 0.12, CHCl3) IR ν: 3300, 3066, 3032, 2981, 2931, 2878, 1651, 1540, 1511, 1224, 1102, 999 cm−1; 1H-NMR (300 MHz, CDCl3) δ: 8.37 (s, 1H, NH), 8.12 (s, 1H, NH), 7.48–6.97 (m, 12H), 6.86 (d, J = 20.7 Hz, 2H, 2ArH), 4.53 (s, 1H, NCH-P), 4.28 (s, 1H, NCH), 4.07 (dd, J = 14.3, 7.2 Hz, 2H, NCH2-Ar + NCH2), 3.97 (s, 2H, 2OCH), 2.97 (d, J = 56.4 Hz, 2H, CH2Ar), 2.29 (s, 2H, 2NH), 1.27–1.11 (m, 12H, 4CH3); 13C-NMR (75 MHz, CDCl3) δ: 183.3, 171.9, 171.3, 163.2, 136.4, 135.9, 134.0, 129.4, 128.5, 128.1, 127.0, 115.4, 115.2, 72.7, 60.5, 53.6, 42.7, 39.3, 21.2; 31P-NMR δ: 19.3; 19F-NMR δ: −115.4 ppm; Anal. Calcd. for C32H40FN4O5PS: C 59.62, H 6.46, N 8.47; Found: C 59.80, H 6.27, N 8.72.
11a: C33H41N4O5PS, white solid, yield 92.6%, m.p. 103–104 °C, [α] D 20 = +115 (c = 0.12, CHCl3) IR ν: 3300, 3087, 3064, 3031, 2985, 2928, 2888, 1677, 1542, 1453, 1394, 1357, 1203, 1025 cm−1; 1H-NMR (300 MHz, CDCl3) δ: 8.81 (s, 1H), 8.58 (s, 1H), 8.43 (s, 1H), 7.47–6.98 (m, 15H), 6.27 (s, 1H), 5.40 (s, 1H), 4.54 (d, J = 57.8 Hz, 2H), 4.24 (s, 1H), 3.78 (d, J = 6.5 Hz, 2H), 3.60 (dd, J = 10.1, 6.6 Hz, 4H), 3.02 (d, J = 3.3 Hz, 2H), 1.92 (dd, J = 68.6, 32.5 Hz, 4H), 0.99 (dt, J = 28.1, 6.9 Hz, 6H); 13C-NMR (75 MHz, CDCl3) δ: 183.3, 172.2, 171.5, 138.6, 136.1, 135.9, 129.4, 128.83, 128.2, 128.0, 127.7, 127.5, 126.8, 64.0, 63.5, 60.2, 57.6, 53.6, 43.0, 42.0, 38.6, 24.9, 18.4, 11.9; 31P-NMR δ: 21.5 ppm; Anal. Calcd. for C33H41N4O5PS: C 62.41, H 6.85, N 8.46; Found: C 62.25, H 6.49, N 8.80.
11b: C35H45N4O5PS, white solid, yield 90.2%, m.p. 117–118 °C, [α] D 20 = +133 (c = 0.13, CHCl3) IR ν: 3423, 3087, 3060, 3033, 2993, 2955, 2887, 1676, 1542, 1456, 1405, 1364, 1204, 1030 cm−1; 1H-NMR (300 MHz, CDCl3) δ: 8.47 (d, J = 5.5 Hz, 2H), 8.23 (s, 1H), 7.53 (s, 1H), 7.33 (s, 1H), 7.27–6.99 (m, 13H), 6.35 (s, 1H), 5.23 (s, 1H), 4.33 (d, J = 19.6 Hz, 2H), 4.20 (s, 1H), 3.62–3.50 (m, 4H), 3.35 (d, J = 26.1 Hz, 2H), 3.10 (s, 2H), 2.75–2.56 (m, 4H), 1.80 (dd, J = 71.0, 33.4 Hz, 3H), 1.49–1.33 (m, 3H); 13C-NMR (75 MHz, CDCl3) δ: 170.8, 161.2, 160.8, 138.5, 134.3, 129.6, 129.2, 128.9, 128.4, 128.2, 127.6, 127.2, 126.7, 118.2, 115.3, 60.5, 58.8, 56.0, 53.4, 52.9, 42.8, 41.9, 38.3, 28.1, 24.6, 22.1, 17.5, 11.7; 31P-NMR δ: 21.2 ppm; Anal. Calcd. for C35H45N4O5PS: C 63.10, H 6.66, N 8.62; Found: C 63.23, H 6.82, N 8.43.
11c: C35H45N4O5PS, white solid, yield 81.8%, m.p. 89–90 °C, [α] D 20 = +89 (c = 0.12, CHCl3) IR ν: 3301, 3085, 3064, 3031, 2983, 2935, 2884, 1675, 1542, 1454, 1386, 1359, 1203, 999 cm−1; 1H-NMR (300 MHz, CDCl3) δ: 8.83 (d, J = 12.3 Hz, 2H), 8.30 (s, 1H), 7.98–6.59 (m, 15H), 6.19 (s, 1H), 5.53 (s, 1H), 4.68 (d, J = 13.0 Hz, 2H), 4.46 (s, 1H), 4.33 (d, J = 15.4 Hz, 2H), 4.16 (s, 1H), 3.36 (s, 1H), 3.23 (s, 1H), 3.05 (s, 1H), 1.98 (dd, J = 81.3, 17.0 Hz, 4H), 1.40–0.88 (m, 12H); 13C-NMR (75 MHz, CDCl3) δ: 183.6, 172.7, 172.6, 139.1, 139.1, 136.6, 129.8, 129.6, 128.8, 128.5, 128.4, 128.4, 127.3, 127.2, 126.7, 72.8, 72.4, 60.3, 57.8, 55.8, 48.2, 43.0, 39.1, 29.6, 25.1, 24.3, 23.5; 31P-NMR δ: 19.9 ppm; Anal. Calcd. for C35H45N4O5PS: C 63.48, H 6.66, N 8.71; Found: C 63.23, H 6.82, N 8.43.
11d: C33H40FN4O5PS, white solid, yield 93.5%, m.p. 97–98 °C, [α] D 20 = +121 (c = 0.11, CHCl3) IR ν: 3302, 3085, 3063, 3031, 2990, 2953, 2887, 1680, 1542, 1453, 1404, 1357, 1203, 1026, 720; 1H-NMR (300 MHz, CDCl3) δ: 9.03–8.50 (m, 2H), 8.33 (s, 1H), 7.19–6.92 (m, 12H), 6.72 (d, J = 8.7 Hz, 2H), 6.14 (s, 1H), 5.22 (s, 1H), 4.56–4.02 (m, 3H), 3.87 (d, J = 7.1 Hz, 2H), 3.64 (dd, J = 19.5, 10.5 Hz, 4H), 3.15 (s, 1H), 2.96 (d, J = 13.5 Hz, 1H), 1.81 (d, J = 9.7 Hz, 2H), 1.69 (d, J = 30.6 Hz, 1H), 0.91–0.81 (td, J = 16.0, 7.9 Hz, 6H); 13C-NMR (75 MHz, CDCl3) δ: 183.0, 171.6, 170.7, 160.5, 135.8, 135.4, 134.2, 128.5, 128.1, 127.6, 127.3, 126.5, 114.7, 114.5, 63.6, 63.1, 63.0, 59.9, 57.4, 41.8, 38.1, 29.1, 24.6, 20.6, 15.7, 13.8; 31P-NMR δ: 21.2; 19F-NMR δ: −115.8 ppm; Anal. Calcd. for C33H40FN4O5PS: C 60.40, H 6.37, N 8.95; Found: C 60.54, H 6.16, N 8.56.
11e: C35H44FN4O5PS, white solid, yield 53.9%, m.p. 107–108 °C, [α] D 20 = +137 (c = 0.13, CHCl3) IR ν: 3296, 3087, 3065, 3031, 2970, 2932, 2882, 1662, 1539, 1454, 1383, 1357, 1222, 1012; 1H-NMR (300 MHz, CDCl3) δ: 8.66–8.49 (m, 2H), 8.35 (s, 1H), 7.45–7.05 (m, 12H), 6.92 (d, J = 8.4 Hz, 2H), 6.10 (s, 1H), 5.25 (s, 1H), 4.51 (s, 1H), 4.30 (d, J = 46.8 Hz, 2H), 3.98 (d, J = 58.8 Hz, 2H), 3.53 (dd, J = 51.6, 32.8 Hz, 4H), 3.05 (s, 1H), 2.92 (s, 1H), 2.20 (s, 1H), 1.96–1.75 (m, 3H), 1.38 (dd, J = 17.7, 7.2 Hz, 2H), 1.21 (t, J = 7.1 Hz, 3H), 0.98–0.60 (m, 3H); 13C-NMR (75 MHz, CDCl3) δ: 173.1, 171.0, 169.5, 160.8, 136.4, 136.1, 134.3, 129.2, 128.6, 127.2, 115.5, 115.3, 69.2, 68.8, 60.3, 59.1, 54.3, 47.3, 45.4, 36.8, 28.4, 25.0, 22.5, 21.1, 10.0, 9.9; 31P-NMR δ: 21.6; 19F-NMR δ: −115.9 ppm; Anal. Calcd. for C35H44FN4O5PS: C 61.34, H 6.77, N 8.02; Found: C 61.57, H 6.50, N 8.21.
11f: C35H44FN4O5PS, white solid, yield 82.4%, m.p. 83–84 °C, [α] D 20 = +107 (c = 0.12, CHCl3) IR ν: 3299, 3087, 3066, 3031, 2980, 2934, 2879, 1676, 1542, 1453, 1386, 1353, 1223, 999; 1H-NMR (300 MHz, CDCl3) δ: 8.73–8.55 (s, 2H), 8.34 (s, 1H), 7.58–7.01 (m, 12H), 6.88 (d, J = 12.5 Hz, 2H), 6.17 (s, 1H), 5.45 (s, 1H), 4.54 (d, J = 37.1 Hz, 2H), 4.30 (s, 1H), 4.06 (d, J = 7.2 Hz, 2H), 3.62 (s, 1H), 3.46 (s, 1H), 3.03 (d, J = 7.5 Hz, 2H), 2.03–1.83 (m, 4H), 1.41–1.30 (m, 3H), 1.20 (t, J = 7.1 Hz, 3H), 1.17–1.08 (m, 3H), 0.86–0.81 (m, 3H); 13C-NMR (75 MHz, CDCl3) δ: 183.3, 172.3, 171.2, 160.6, 136.4, 136.0, 135.9, 128.9, 128.5, 127.7, 126.9, 115.2, 115.1, 72.6, 72.5, 60.4, 57.6, 53.6, 42.3, 42.0, 38.9, 28.3, 24.9, 24.2, 24.1, 23.2, 21.1; 31P-NMR δ: 19.8; 19F-NMR δ: −115.7 ppm; Anal. Calcd. for C35H44FN4O5PS: C 61.36, H 6.82, N 8.00; Found: C 61.57, H 6.50, N 8.21.

3.3. Antitumor Activity

All tested compounds were dissolved in DMSO and subsequently diluted in the culture medium in indicated final concentrations before treatment of the cultured cells. Tested cells were plated in 96-well plates at a density 2 × 104 cells/well/100 µL of the proper culture medium and were treated with the compounds at 3.9–500 µM for 72 h. In parallel, the cells treated with 0.1% DMSO served as a control. An MTT [3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide] assay was performed 4 h later, according to the instructions. This assay was based on the cellular cleavage of MTT into formazane which is soluble in the cell culture medium. Any absorbance caused by formazan was measured at 595 nm with a microplate reader (BIO-RAD, model 680), which was directly proportional to the number of living cells in the culture. Two types of cells were used in these assays, BGC-823 (human gastric cancer) and A-549 (non-small cell lung cancer) cell lines, provided by ATCC (American Type Culture Collection) and cultivated in RPMI 1640 (for BGC-823 and A-549) supplemented with 10% fetal bovine serum. Tissue culture reagents were obtained from Gibco BRL. The experiment was performed in triplicate.

4. Conclusions

In summary, this study is based on our previous work [19]. Our attempt was to incorporate glycine or rigid amino acids such as L-proline into the previous pseudo-peptide thiourea containing an α-aminophosphonate moiety. A series of novel chiral dipeptide thioureas containing α-aminophosphonate moieties 10al and 11af were synthesized in high yield (Table 1). All these novel thioureas could inhibit tumor cell lines (BGC-823 and A-549) below 100 µM (Table 2) by the MTT assay. Although MTT is not a direct reflection on cell proliferation specifically and other orthogonal assays may need to be performed [27], the thioureas 11d and 11f showed comparable inhibition with that of Cisplatin against BGC-823 and A-549 cells, respectively (Table 2). Finally, we can conclude their structure-activity relationship; overall, the rigid amino acid of incorporated L-proline is helpful for antitumor activity. L-phenylalanine containing thioureas also showed better antitumor activities than that of L-phenylglycine containing thioureas. Our results also indicate that the antitumor activity could be improved by introducing an electron-withdrawing group in the para position of the terminal phenyl group of the dipeptide thioureas. This will guide us to design and obtain more and more potent antitumor agents. The mechanism of antitumor activity for these novel dipeptide thioureas is under further investigation.

Acknowledgments

We are grateful for financial support from the National Natural Science Foundation of China (Nos. 81160385, 21302027 and 21662010), One Thousand Talents Program of Guizhou Province (2013), the Innovation Team of Natural Science Foundation of the Department of Education of Guizhou Province (No. QJHRCTDZ[2015]57), the Projects of Natural Science Foundation of the Department of Education of Guizhou Province (No. QJHKYZ[2015]371, QKHSYZ[2012]3103 and QSZHZ[2012]89), and the Innovation Team of Science and Technology of the Natural Drug Resources Research of Guizhou Province (No. QKHRCTD[2015]4025).

Author Contributions

J.L. and Y.W. conceived and designed the experiments; P.L. and J.H. performed the experiments; Y.L. (Yongjun Li) and H.Z. analyzed the data; B.H. and Y.L. (Yan Li) wrote the paper.

Conflicts of Interest

The authors declare no conflict of interest.

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  • Sample Availability: Samples of the compounds 11d and 11f are available from the authors.
Figure 1. Synthetic route for novel thioureas 10 and 11.
Figure 1. Synthetic route for novel thioureas 10 and 11.
Molecules 22 00238 g001
Table 1. Structure, yield, and melting point of novel thioureas 10 and 11.
Table 1. Structure, yield, and melting point of novel thioureas 10 and 11.
CompoundXR1R2Yield (%) m.p. (°C)
10aHL-PhEt91.6179–180
10bHL-Phn-Pr81.5155–157
10cHL-Phi-Pr78.6203–204
10d4-FL-PhEt93.374–75
10e4-FL-Phn-Pr75.5133–134
10f4-FL-Phi-Pr77.8176–177
10gHL-BnEt66.8167–168
10hHL-Bnn-Pr71.4148–149
10iHL-Bni-Pr70.472–73
10j4-FL-BnEt98.775–76
10k4-FL-Bnn-Pr94.395–96
10l4-FL-Bni-Pr95.062–63
11aHL-BnEt92.6103–104
11bHL-Bnn-Pr90.2117–118
11cHL-Bni-Pr81.889–90
11d4-FL-BnEt93.597–98
11e4-FL-Bnn-Pr53.9107–108
11f4-FL-Bni-Pr82.483–84
Table 2. IC50 values of thioureas 10 and 11 for BGC-823 and A-549 cells.
Table 2. IC50 values of thioureas 10 and 11 for BGC-823 and A-549 cells.
CompoundXR1R2IC50/(μmol·L−1)
BGC-823A-549
10aHL-PhEt54.8 ± 3.263.2 ± 2.1
10bHL-Phn-Pr87.3 ± 7.1112.5 ± 7.9
10cHL-Phi-Pr61.5 ± 2.274.3 ± 6.6
10d4-FL-PhEt51.9 ± 3.551.5 ± 3.8
10e4-FL-Phn-Pr58.1 ± 2.056.2 ± 4.2
10f4-FL-Phi-Pr47.2 ± 4.343.4 ± 3.1
10gHL-BnEt53.5 ± 1.867.5 ± 3.4
10hHL-Bnn-Pr103.6 ± 8.958.3 ± 4.1
10iHL-Bni-Pr42.7 ± 2.146.1 ± 3.3
10j4-FL-BnEt38.3 ± 3.335.2 ± 2.5
10k4-FL-Bnn-Pr44.1 ± 3.149.5 ± 5.1
10l4-FL-Bni-Pr24.8 ± 2.641.7 ± 3.0
11aHL-BnEt31.4 ± 2.023.7 ± 2.2
11bHL-Bnn-Pr45.2 ± 1.634.6 ± 2.1
11cHL-Bni-Pr39.7 ± 3.340.8 ± 3.9
11d4-FL-BnEt20.9 ± 2.830.3 ± 1.8
11e4-FL-Bnn-Pr37.5 ± 3.729.5 ± 3.4
11f4-FL-Bni-Pr25.6 ± 4.119.2 ± 2.3
Cisplatin 15.1 ± 2.317.6 ± 3.1

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MDPI and ACS Style

Liu, J.; Liao, P.; Hu, J.; Zhu, H.; Wang, Y.; Li, Y.; Li, Y.; He, B. Synthesis and Antitumor Activities of Chiral Dipeptide Thioureas Containing an Alpha-Aminophosphonate Moiety. Molecules 2017, 22, 238. https://doi.org/10.3390/molecules22020238

AMA Style

Liu J, Liao P, Hu J, Zhu H, Wang Y, Li Y, Li Y, He B. Synthesis and Antitumor Activities of Chiral Dipeptide Thioureas Containing an Alpha-Aminophosphonate Moiety. Molecules. 2017; 22(2):238. https://doi.org/10.3390/molecules22020238

Chicago/Turabian Style

Liu, Jingzi, Peng Liao, Junfeng Hu, Hong Zhu, Yonglin Wang, Yongjun Li, Yan Li, and Bin He. 2017. "Synthesis and Antitumor Activities of Chiral Dipeptide Thioureas Containing an Alpha-Aminophosphonate Moiety" Molecules 22, no. 2: 238. https://doi.org/10.3390/molecules22020238

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