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Int. J. Mol. Sci. 2010, 11(4), 1236-1252; doi:10.3390/ijms11041236

Whole Cell Imprinting in Sol-Gel Thin Films for Bacterial Recognition in Liquids: Macromolecular Fingerprinting

Faculty of Civil & Environmental Engineering, Division of Environmental, Water and Agricultural Engineering, Technion, Haifa 32000, Israel
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Received: 28 January 2010 / Revised: 26 February 2010 / Accepted: 22 March 2010 / Published: 24 March 2010
(This article belongs to the Special Issue Sol-Gel Technique)
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Abstract

Thin films of organically modified silica (ORMOSILS) produced by a sol-gel method were imprinted with whole cells of a variety of microorganisms in order to develop an easy and specific probe to concentrate and specifically identify these microorganisms in liquids (e.g., water). Microorganisms with various morphology and outer surface components were imprinted into thin sol-gel films. Adsorption of target microorganism onto imprinted films was facilitated by these macromolecular fingerprints as revealed by various microscopical examinations (SEM, AFM, HSEM and CLSM). The imprinted films showed high selectivity toward each of test microorganisms with high adsorption affinity making them excellent candidates for rapid detection of microorganisms from liquids.
Keywords: sol-gel; bacteria; protozoan parasite; whole cell imprinting; biosensors; sol-gel films sol-gel; bacteria; protozoan parasite; whole cell imprinting; biosensors; sol-gel films
This is an open access article distributed under the Creative Commons Attribution License (CC BY 3.0).

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Cohen, T.; Starosvetsky, J.; Cheruti, U.; Armon, R. Whole Cell Imprinting in Sol-Gel Thin Films for Bacterial Recognition in Liquids: Macromolecular Fingerprinting. Int. J. Mol. Sci. 2010, 11, 1236-1252.

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