A Determination of Potential α-Glucosidase Inhibitors from Azuki Beans (Vigna angularis)
Abstract
:1. Introduction
2. Results and Discussion
2.1. Isolation of Active Compounds and Structural Determination
2.2. Alpha-Glucosidase Inhibition Activities
2.3. Fluorescence Spectra
3. Materials and Methods
3.1. Materials
3.2. Isolation and Identification of Active Compounds
3.3. Evaluation of α-Glucosidase Inhibitory Activity
3.4. Measurement of Fluorescence Spectra
4. Conclusion
Acknowledgements
References
- Yao, Y; Ren, G; Wang, JS; Chen, F; Wang, MF. Antidiatbetic of mung bean extracts in diabetic KK- Ay mice. J. Agric. Food Chem 2008, 56, 8869–8873. [Google Scholar]
- Krentz, AJ; Bailey, CJ. Oral antidiabetic agents current role in type 2 diabetes mellitus. Drugs 2005, 65, 385–411. [Google Scholar]
- Lebovitz, HE. Postprandial hyperglycemic state: Importance and consequences. Diabetes Res. Clin. Pract 1998, 40, 27–28. [Google Scholar]
- Puls, W; Keup, U; Krause, HP; Thomas, G; Hofmeister, F. Glucosidase inhibition: A new approach to the treatment of diabetes, obesity, and hyperlipoproteinaemia. Naturwissenschaften 1977, 64, 536–537. [Google Scholar]
- Lee, SH; Chun, HK; Park, HJ; Lee, YS. Supplementary effect of the high dietary fiber rice on blood glucose in diabetic KK-Ay mice. Korean J. Nutr 2004, 37, 75–80. [Google Scholar]
- Lin, PY; Lai, HM. Bioactive compounds in legumes and their germinated products. J. Agric. Food Chem 2006, 54, 3807–3814. [Google Scholar]
- Itoh, T; Kita, N; Kurokawa, Y; Kobayashi, M; Horio, F; Furuichi, Y. Suppressive effect of a hot water extract of adzuki beans (Vigna angularis) on hyperglycemia after sucrose loading in mice and diabetic rats. Biosci. Biotechnol. Biochem 2004, 68, 2421–2416. [Google Scholar]
- Hosoya, T; Yun, YS; Kunugi, A. Five novel flavonoids from Wasabia japonica. Tetrahedron 2005, 61, 7037–7044. [Google Scholar]
- Tanaka, JCA; Conceicao da Silva, C; Dias Filho, BP; Nakamura, V; Ernesto de Carvalho, J; Foglio, MA. Chemical constituents of Luehea divaricata Mart. (Tiliaceae). Quimica Nova 2005, 28, 834–837. [Google Scholar]
- Yang, P; Gao, F. Principles of Bioinorganic Chemistry; Science Press: Beijing, China, 2002; pp. 331–332. [Google Scholar]
- Zhang, XW; Zhao, FL; Li, KA. Progress in the research of the interaction between drug and serum protein in vitro. Chemistry 1999, 20, 1063–1067. [Google Scholar]
- Li, YQ; Zhou, FC; Gao, F; Bian, JS; Shan, F. Comparative evaluation of quercetin, isoquercetin and rutin as inhibitors of α-glucosidase. J. Agric. Food Chem 2009, 57, 11463–11468. [Google Scholar]
- Xie, MY; Jiang, M; Li, S; Liu, Y. Study on the interaction of β-1,2,3,4,6-Penta-O-galloyl- d-glucopyranose with human serum albumin. Acta Chem. Sin 2004, 62, 1460–1466. [Google Scholar]
- Nishioka, T; Wabata, J; Aoyama, Y. Baicalein, α-glucosidase inhibitor from Scutellaria baicalensis. J. Nat. Prod 1998, 61, 1413–1415. [Google Scholar]
- Yao, Y; Wei, S; Meijing, Z; Ren, G. Antioxidant and α-glucosidase inhibitory activity of colored grains in China. J. Agric. Food Chem 2010, 58, 770–774. [Google Scholar]
Extracts/compounds | IC50 |
---|---|
CH2Cl2-soluble | >500 |
EtOAc-soluble | 53.74 |
n-BuOH-soluble, | 173.69 |
Residual extract | >500 |
Vitexin | 0.4 |
Isovitexin | 4.8 |
Acarbose | 0.45 |
T (°C) | KSV/105 (L·mol−1) | Kq/1013 (L·mol−1·S−1) | R2 | |
---|---|---|---|---|
Vitexin | 25 | 1.38 | 1.38 | 0.9436 |
37 | 1.13 | 1.13 | 0.9627 | |
Isovitexin | 25 | 1.06 | 1.06 | 0.9829 |
37 | 0.98 | 0.98 | 0.9807 |
T (°C) | KA/105 (L·mol−1) | n | R2 | |
---|---|---|---|---|
Vitexin | 25 | 1.23 | 1.21 | 0.9865 |
37 | 1.37 | 1.24 | 0.9940 | |
Isovitexin | 25 | 1.19 | 1.09 | 0.9671 |
37 | 1.25 | 1.17 | 0.9513 |
© 2011 by the authors; licensee MDPI, Basel, Switzerland. This article is an open-access article distributed under the terms and conditions of the Creative Commons Attribution license (http://creativecommons.org/licenses/by/3.0/).
Share and Cite
Yao, Y.; Cheng, X.; Wang, L.; Wang, S.; Ren, G. A Determination of Potential α-Glucosidase Inhibitors from Azuki Beans (Vigna angularis). Int. J. Mol. Sci. 2011, 12, 6445-6451. https://doi.org/10.3390/ijms12106445
Yao Y, Cheng X, Wang L, Wang S, Ren G. A Determination of Potential α-Glucosidase Inhibitors from Azuki Beans (Vigna angularis). International Journal of Molecular Sciences. 2011; 12(10):6445-6451. https://doi.org/10.3390/ijms12106445
Chicago/Turabian StyleYao, Yang, Xuzhen Cheng, Lixia Wang, Suhua Wang, and Guixing Ren. 2011. "A Determination of Potential α-Glucosidase Inhibitors from Azuki Beans (Vigna angularis)" International Journal of Molecular Sciences 12, no. 10: 6445-6451. https://doi.org/10.3390/ijms12106445