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Int. J. Mol. Sci. 2012, 13(12), 17275-17289; doi:10.3390/ijms131217275

Optimization of Synthesis, Characterization and Cytotoxic Activity of Seleno-Capparis spionosa L. Polysaccharide

1
Research Center on Life Sciences and Environmental Sciences, Harbin University of Commerce, Harbin 150076, China
2
Engineering Research Center of Natural Anticancer Drugs, Ministry of Education, Harbin 150076, China
*
Author to whom correspondence should be addressed.
Received: 21 August 2012 / Revised: 17 November 2012 / Accepted: 4 December 2012 / Published: 17 December 2012
(This article belongs to the Section Green Chemistry)
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Abstract

In this study, an experiment was designed to optimize the synthesis of seleno-Capparis spionosa L. polysaccharide (Se-CSPS) by response surface methodology. Three independent variables (reaction time, reaction temperature and ratio of Na2SeO3 to CSPS) were tested. Furthermore, the thermal stability, particle size, shape and cytotoxic activity of Se-CSPS in vitro were investigated. The optimum reaction conditions were obtained shown as follows: reaction time 7.5 h, reaction temperature 71 °C, and ratio of Na2SeO3 to CSPS 0.9 g/g. Under these conditions, the Se content in Se-CSPS reached 5.547 mg/g, which was close to the predicted value (5.518 mg/g) by the model. The thermal stability, particle size and shape of Se-CSPS were significantly different from those of CSPS. Additionally, a MTT assay indicated that the Se-CSPS could inhibit the proliferation of human gastric cancer SGC-7901 cells in a dose-dependent manner. View Full-Text
Keywords: seleno-Capparis spionosa L. polysaccharide(Se-CSPS); reaction optimization; response surface methodology; characterization; cytotoxic activity seleno-Capparis spionosa L. polysaccharide(Se-CSPS); reaction optimization; response surface methodology; characterization; cytotoxic activity
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MDPI and ACS Style

Ji, Y.-B.; Dong, F.; Lang, L.; Zhang, L.-W.; Miao, J.; Liu, Z.-F.; Jin, L.-N.; Hao, Y. Optimization of Synthesis, Characterization and Cytotoxic Activity of Seleno-Capparis spionosa L. Polysaccharide. Int. J. Mol. Sci. 2012, 13, 17275-17289.

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