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Int. J. Mol. Sci. 2013, 14(10), 19474-19483; doi:10.3390/ijms141019474

Parts Per Trillion Detection of 7-Aminonitrazepam by Nano-Enhanced ELISA

1,* , 1
1 State Key Lab of Food Science and Technology, School of Food Science and Technology, Jiangnan University, Wuxi 214122, China 2 Animal, Plant & Food Inspection Center, Jiangsu Entry-Exit Inspection and Quarantine Bureau, Nanjing 200001, China
* Author to whom correspondence should be addressed.
Received: 10 July 2013 / Revised: 6 September 2013 / Accepted: 12 September 2013 / Published: 25 September 2013
(This article belongs to the Section Molecular Diagnostics)
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It is challenging to detect 7-aminonitrazepam (7-ANZP) residue in animal tissues simply and sensitively by the enzyme-linked sorbent immunoassay (ELISA) method. This paper demonstrates that utilizing a bioconjugate of gold nanoparticles and enzyme-labeled antibody as a signal probe increases the sensitivity of a traditional ELISA for 7-ANZP by nearly 20 times. The sensitivity of this ELISA for 7-ANZP was 5.6 pg/mL in buffer, and the limit of detection (LOD) of 0.18 µg/kg for 7-ANZP in urine could be achieved after the urine samples were simply hydrolyzed and diluted by buffer. This simple and sensitive method has potential application for improving the sensitivity of ELISA methods against various small molecules.
Keywords: 7-aminonitrazepam; ELISA; gold nanoparticle; bioconjugate 7-aminonitrazepam; ELISA; gold nanoparticle; bioconjugate
This is an open access article distributed under the Creative Commons Attribution License (CC BY) which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Peng, C.; Duan, X.; Song, S.; Xue, F. Parts Per Trillion Detection of 7-Aminonitrazepam by Nano-Enhanced ELISA. Int. J. Mol. Sci. 2013, 14, 19474-19483.

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