Int. J. Mol. Sci. 2013, 14(10), 20414-20426; doi:10.3390/ijms141020414
Characterization of 42 Microsatellite Markers from Poison Ivy, Toxicodendron radicans (Anacardiaceae)
1
Endemic Species Research Institute, Nantou 552, Taiwan
2
Department of Nursing, Meiho University, Pingtung 912, Taiwan
3
Department of Life Sciences, National Cheng Kung University, Tainan 701, Taiwan
4
Department of Biological Sciences, National Sun Yat-sen University, Kaohsiung 804, Taiwan
*
Authors to whom correspondence should be addressed.
Received: 21 August 2013 / Revised: 22 September 2013 / Accepted: 23 September 2013 / Published: 14 October 2013
(This article belongs to the Section Biochemistry, Molecular Biology and Biophysics)
Abstract
Poison ivy, Toxicodendron radicans, and poison oaks, T. diversilobum and T. pubescens, are perennial woody species of the Anacardiaceae and are poisonous, containing strong allergens named urushiols that cause allergic contact dermatitis. Poison ivy is a species distributed from North America to East Asia, while T. diversilobum and T. pubescens are distributed in western and eastern North America, respectively. Phylogreography and population structure of these species remain unclear. Here, we developed microsatellite markers, via constructing a magnetic enriched microsatellite library, from poison ivy. We designed 51 primer pairs, 42 of which successfully yielded products that were subsequently tested for polymorphism in poison oak, and three subspecies of poison ivy. Among the 42 loci, 38 are polymorphic, while 4 are monomorphic. The number of alleles and the expected heterozygosity ranged from 1 to 12 and from 0.10 to 0.87, respectively, in poison ivy, while varied from 2 to 8 and, from 0.26 to 0.83, respectively in poison oak. Genetic analysis revealed distinct differentiation between poison ivy and poison oak, whereas slight genetic differentiation was detected among three subspecies of poison ivy. These highly polymorphic microsatellite fingerprints enable biologists to explore the population genetics, phylogeography, and speciation in Toxicodendron.Keywords:
genetic diversity; microsatellite markers; poison ivy; poison oak; population structuring; Toxicodendron
This is an open access article distributed under the Creative Commons Attribution License (CC BY 3.0).
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MDPI and ACS Style
Hsu, T.-W.; Shih, H.-C.; Kuo, C.-C.; Chiang, T.-Y.; Chiang, Y.-C. Characterization of 42 Microsatellite Markers from Poison Ivy, Toxicodendron radicans (Anacardiaceae). Int. J. Mol. Sci. 2013, 14, 20414-20426.
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