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Int. J. Mol. Sci. 2013, 14(10), 20913-20929; doi:10.3390/ijms141020913

Overexpression of Ferredoxin, PETF, Enhances Tolerance to Heat Stress in Chlamydomonas reinhardtii

1
Department of Plant Medicine, National Pingtung University of Science and Technology, Pingtung 912, Taiwan
2
Graduate School of Biotechnology and Bioengineering, Yuan Ze University, Taoyuan 320, Taiwan
3
Institute of Plant and Microbial Biology, Academia Sinica, Taipei 115, Taiwan
4
Department of Life Science, National Taitung University, Taitung 684, Taiwan
*
Author to whom correspondence should be addressed.
Received: 31 August 2013 / Revised: 10 October 2013 / Accepted: 11 October 2013 / Published: 17 October 2013
(This article belongs to the Section Biochemistry, Molecular Biology and Biophysics)
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Abstract

Reactive oxygen species (ROS) produced by plants in adverse environments can cause damage to organelles and trigger cell death. Removal of excess ROS can be achieved through the ascorbate scavenger pathway to prevent plant cell death. The amount of this scavenger can be regulated by ferredoxin (FDX). Chloroplastic FDXs are electron transfer proteins that perform in distributing photosynthetic reducing power. In this study, we demonstrate that overexpression of the endogenous photosynthetic FDX gene, PETF, in Chlamydomonas reinhardtii could raise the level of reduced ascorbate and diminish H2O2 levels under normal growth conditions. Furthermore, the overexpressing PETF transgenic Chlamydomonas lines produced low levels of H2O2 and exhibited protective effects that were observed through decreased chlorophyll degradation and increased cell survival under heat-stress conditions. The findings of this study suggest that overexpression of PETF can increase the efficiency of ROS scavenging in chloroplasts to confer heat tolerance. The roles of PETF in the downregulation of the ROS level offer a method for potentially improving the tolerance of crops against heat stress. View Full-Text
Keywords: Chlamydomonas reinhardtii; ferredoxin; PETF; heat tolerance; ascorbate Chlamydomonas reinhardtii; ferredoxin; PETF; heat tolerance; ascorbate
This is an open access article distributed under the Creative Commons Attribution License (CC BY 3.0).

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MDPI and ACS Style

Lin, Y.-H.; Pan, K.-Y.; Hung, C.-H.; Huang, H.-E.; Chen, C.-L.; Feng, T.-Y.; Huang, L.-F. Overexpression of Ferredoxin, PETF, Enhances Tolerance to Heat Stress in Chlamydomonas reinhardtii. Int. J. Mol. Sci. 2013, 14, 20913-20929.

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Int. J. Mol. Sci. EISSN 1422-0067 Published by MDPI AG, Basel, Switzerland RSS E-Mail Table of Contents Alert
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