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Int. J. Mol. Sci. 2013, 14(11), 22449-22461; doi:10.3390/ijms141122449

Evaluation of the Comet Assay for Assessing the Dose-Response Relationship of DNA Damage Induced by Ionizing Radiation

1
Institute of Radiation Medicine, Chinese Academy of Medical Sciences and Peking Union Medical College, Tianjin 300192, China
2
Tianjin Key Laboratory of Molecular Nuclear Medicine, First Clinical Department of Medical Emergency Response Center for Nuclear Accidents, Ministry of Health, Tianjin 300192, China
3
National Institute for Radiological Protection and Nuclear Safety, Chinese Center for Disease Control, Beijing 100088, China
4
Medical Emergency Response Center for Nuclear Accidents, Ministry of Health, Beijing 100088, China
5
Tianjin Key Laboratory of Food and Biotechnology, Tianjin University of Commerce, Tianjin 300134, China
6
National Institute of Radiological Sciences, Chiba 263-8555, Japan
These authors contributed equally to this work.
*
Author to whom correspondence should be addressed.
Received: 4 September 2013 / Revised: 25 October 2013 / Accepted: 30 October 2013 / Published: 14 November 2013
(This article belongs to the Collection Radiation Toxicity in Cells)
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Abstract

Dose- and time-response curves were combined to assess the potential of the comet assay in radiation biodosimetry. The neutral comet assay was used to detect DNA double-strand breaks in lymphocytes caused by γ-ray irradiation. A clear dose-response relationship with DNA double-strand breaks using the comet assay was found at different times after irradiation (p < 0.001). A time-response relationship was also found within 72 h after irradiation (p < 0.001). The curves for DNA double-strand breaks and DNA repair in vitro of human lymphocytes presented a nice model, and a smooth, three-dimensional plane model was obtained when the two curves were combined. View Full-Text
Keywords: radiation; dose-response relationship; DNA damage; comet assay radiation; dose-response relationship; DNA damage; comet assay
This is an open access article distributed under the Creative Commons Attribution License (CC BY 3.0).

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MDPI and ACS Style

Wang, Y.; Xu, C.; Du, L.Q.; Cao, J.; Liu, J.X.; Su, X.; Zhao, H.; Fan, F.-Y.; Wang, B.; Katsube, T.; Fan, S.J.; Liu, Q. Evaluation of the Comet Assay for Assessing the Dose-Response Relationship of DNA Damage Induced by Ionizing Radiation. Int. J. Mol. Sci. 2013, 14, 22449-22461.

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