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Int. J. Mol. Sci. 2013, 14(2), 4349-4360; doi:10.3390/ijms14024349

PG-2, a Potent AMP against Pathogenic Microbial Strains, from Potato (Solanum tuberosum L cv. Gogu Valley) Tubers Not Cytotoxic against Human Cells

Research Center for Proteineous Materials, Chosun University, Gwangju 501-759, Korea
Department of Bioinformatics, Kongju National University, Kongju 314-701, Korea
College of Agriculture and Life Sciences, Chonnam National University, Gwangju 501-759, Korea
Department of Biotechnology, Chosun University, Gwangju 501-759, Korea
Author to whom correspondence should be addressed.
Received: 16 December 2012 / Revised: 16 January 2013 / Accepted: 6 February 2013 / Published: 21 February 2013
(This article belongs to the Special Issue Plant-Derived Pharmaceuticals by Molecular Farming 2012)
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In an earlier study, we isolated potamin-1 (PT-1), a 5.6-kDa trypsin-chymotrypsin protease inhibitor, from the tubers of a potato strain (Solanum tuberosum L cv. Gogu Valley). We established that PT-1 strongly inhibits pathogenic microbial strains, but not human bacterial strains, and that its sequence shows 62% homology with a serine protease inhibitor. In the present study, we isolated an antifungal and antibacterial peptide with no cytotoxicity from tubers of the same potato strain. The peptide (peptide-G2, PG-2) was isolated using salt-extraction, ultrafiltration and reverse-phase high performance liquid chromatography (RP-HPLC). Matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF/MS) showed the protein to have a molecular mass of 3228.5 Da, while automated Edman degradation showed the N-terminal sequence of PG-2 to be LVKDNPLDISPKQVQALCTDLVIRCMCCC-. PG-2 exhibited antimicrobial activity against Candida albicans, a human pathogenic yeast strain, and Clavibacter michiganensis subsp. michiganensis, a plant late blight strain. PG-2 also showed antibacterial activity against Staphylococcus aureus, but did not lyse human red blood cells and was thermostable. Overall, these results suggest PG-2 may be a good candidate to serve as a natural antimicrobial agent, agricultural pesticide and/or food additive.
Keywords: peptide-G2; RP-HPLC; N-terminal sequence; human pathogenic; antimicrobial agent peptide-G2; RP-HPLC; N-terminal sequence; human pathogenic; antimicrobial agent
This is an open access article distributed under the Creative Commons Attribution License (CC BY 3.0).

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Kim, J.-Y.; Gopal, R.; Kim, S.Y.; Seo, C.H.; Lee, H.B.; Cheong, H.; Park, Y. PG-2, a Potent AMP against Pathogenic Microbial Strains, from Potato (Solanum tuberosum L cv. Gogu Valley) Tubers Not Cytotoxic against Human Cells. Int. J. Mol. Sci. 2013, 14, 4349-4360.

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