Next Article in Journal / Special Issue
Cadmium-Induced Pathologies: Where Is the Oxidative Balance Lost (or Not)?
Previous Article in Journal
PAX2 Expression in Ovarian Cancer
Previous Article in Special Issue
The Role of Metallothionein in Oxidative Stress
Article Menu

Export Article

Open AccessArticle
Int. J. Mol. Sci. 2013, 14(3), 6106-6115; doi:10.3390/ijms14036106

Comparative Analysis of Serum (Anti)oxidative Status Parаmeters in Healthy Persons

1
Center for Health Protection, National Institute for Public Health and the Environment, Bilthoven, The Netherlands
2
Faculty of Medical Sciences, Goce Delcev University, Stip 2000, Macedonia
*
Author to whom correspondence should be addressed.
Received: 31 December 2012 / Revised: 5 March 2013 / Accepted: 7 March 2013 / Published: 18 March 2013
(This article belongs to the Special Issue Redox Signaling in Biology and Patho-Biology)
View Full-Text   |   Download PDF [204 KB, uploaded 19 June 2014]

Abstract

Five antioxidant and two oxidative stress assays were applied to serum samples of 43 healthy males. The antioxidant tests showed different inter-assay correlations. A very good correlation of 0.807 was observed between the ferric reducing ability of plasma (FRAP) and total antioxidant status (TAS) assay and also a fair correlation of 0.501 between the biological antioxidant potential (BAP) and TAS assay. There was no statistically significant correlation between the BAP and FRAP assay. The anti-oxidant assays have a high correlation with uric acid, especially the TAS (0.922) and FRAP assay (0.869). The BAP assay has a much lower and no statistically significant correlation with uric acid (0.302), which makes BAP more suitable for the antioxidant status. The total thiol assay showed no statistically significant correlation with uric acid (0.114). The total thiol assay, which is based on a completely different principle, showed a good and statistically significant correlation with the BAP assay (0.510) and also to the TAS assay, but to a lower and not significant extent (0.279) and not with the FRAP assay (−0.008). The oxy-adsorbent test (OXY) assay has no correlation with any of the other assays tested. The oxidative stress assays, reactive oxygen metabolites (ROM) and total oxidant status (TOS), based on a different principle, do not show a statistically significant correlation with the serum samples in this study. Both assays showed a negative, but not significant, correlation with the antioxidant assays. In conclusion, the ROM, TOS, BAP and TTP assays are based on different principles and will have an additional value when a combination of these assays will be applied in large-scale population studies. View Full-Text
Keywords: oxidation assays; antioxidant assays; total thiol assay; correlation oxidation assays; antioxidant assays; total thiol assay; correlation
This is an open access article distributed under the Creative Commons Attribution License (CC BY 3.0).

Scifeed alert for new publications

Never miss any articles matching your research from any publisher
  • Get alerts for new papers matching your research
  • Find out the new papers from selected authors
  • Updated daily for 49'000+ journals and 6000+ publishers
  • Define your Scifeed now

SciFeed Share & Cite This Article

MDPI and ACS Style

Jansen, E.H.; Ruskovska, T. Comparative Analysis of Serum (Anti)oxidative Status Parаmeters in Healthy Persons. Int. J. Mol. Sci. 2013, 14, 6106-6115.

Show more citation formats Show less citations formats

Related Articles

Article Metrics

Article Access Statistics

1

Comments

[Return to top]
Int. J. Mol. Sci. EISSN 1422-0067 Published by MDPI AG, Basel, Switzerland RSS E-Mail Table of Contents Alert
Back to Top