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Int. J. Mol. Sci. 2013, 14(5), 8719-8739; doi:10.3390/ijms14058719

Improving Pharmaceutical Protein Production in Oryza sativa

Graduate School of Biotechnology and Bioengineering, Yuan Ze University, 135 Yuan-Tung Road, Taoyuan 32003, Taiwan
Department of Life Sciences, National Central University, 300, Jhongda Rd., Taoyuan 32001, Taiwan
Author to whom correspondence should be addressed.
Received: 21 January 2013 / Revised: 14 April 2013 / Accepted: 15 April 2013 / Published: 24 April 2013
(This article belongs to the Special Issue Plant-Derived Pharmaceuticals by Molecular Farming 2012)
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Application of plant expression systems in the production of recombinant proteins has several advantages, such as low maintenance cost, absence of human pathogens, and possession of complex post-translational glycosylation capabilities. Plants have been successfully used to produce recombinant cytokines, vaccines, antibodies, and other proteins, and rice (Oryza sativa) is a potential plant used as recombinant protein expression system. After successful transformation, transgenic rice cells can be either regenerated into whole plants or grown as cell cultures that can be upscaled into bioreactors. This review summarizes recent advances in the production of different recombinant protein produced in rice and describes their production methods as well as methods to improve protein yield and quality. Glycosylation and its impact in plant development and protein production are discussed, and several methods of improving yield and quality that have not been incorporated in rice expression systems are also proposed. Finally, different bioreactor options are explored and their advantages are analyzed.
Keywords: bioreactor; glycosylation; inducible system; Oryza sativa; recombinant protein; suspension cells; transgenic plants bioreactor; glycosylation; inducible system; Oryza sativa; recombinant protein; suspension cells; transgenic plants
This is an open access article distributed under the Creative Commons Attribution License (CC BY 3.0).

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MDPI and ACS Style

Kuo, Y.-C.; Tan, C.-C.; Ku, J.-T.; Hsu, W.-C.; Su, S.-C.; Lu, C.-A.; Huang, L.-F. Improving Pharmaceutical Protein Production in Oryza sativa. Int. J. Mol. Sci. 2013, 14, 8719-8739.

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