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Int. J. Mol. Sci. 2013, 14(9), 18502-18519; doi:10.3390/ijms140918502
Abstract: The Paeonia root, with or without bark, are considered vital traditional Chinese medicine materials; the examples are those of Bai Shao, Chi Shao, and Dan Pi. In this study, we examine 24 genes and their expressions involved in the biosynthesis of paeoniflorin and its derivatives, which are active compounds of the Paeonia root, in Paeonia lactiflora and P. suffruticosa, as well as other related plants, Punica granatum, Rhus radicans, and Coriaria nepalensis. Our phylogenetic analyses suggest that these genes have functional diversity, and analysis of the transcriptional level shows paeoniflorin and gallic acid biosynthesis-related genes exhibit different transcription profiles in flowers, carpels, bark-free roots, and bark of P. lactiflora. The correlation analysis of gene expression and active compound contents support the idea that hydroxymethylglutaryl-CoA synthase and phosphomevalonate kinase in the mevalonate pathway and 3-dehydroquinate dehydratase/shikimate dehydrogenase in shikimate biosynthesis are potentially closely related to the accumulation of paeoniflorin and benzoylpaeoniflorin. Coupling gene diversity with chemical analysis, we show that paeoniflorin and its derived aromatic amino acids are predominant in bark.
In China, Korea, and Japan, a decoction of the dried root of Shao Yao (Paeonia lactiflora Pall.) is used to treat rheumatoid arthritis, systemic lupus erythematosus, hepatitis, dysmenorrhea, muscle cramps and spasms, and fever for more than 1200 years . In modern herb markets, the dry bark-free root of cultivated P. lactiflora is called Bai Shao (literally, “White Peony”) and the dry root with bark of the wild P. lactiflora is called Chi Shao (literally, “Red Peony”). However, consensus on their characteristics, including flower color, region of cultivation, medicinal processing, and origin of domestication, has yet to be reached . Dan Pi (literally, “Tree Peony Bark”), another traditional Chinese medicine material, is derived from the root cortex of P. suffruticosa and serves as a remedy for various diseases, including macula, epilepsy, and menstrual disorders .
Differences between Bai Shao, Chi Shao, and Dan Pi can be attributed to the functional difference of the barks. A water/ethanol extract of Bai Shao has been found to contain all glucosides of peony, which include paeoniflorin, albiflorin, oxypaeoniflorin, benzoylpaeoniflorin, oxybenzoylpaeoniflorin, paeoniflorigenone, lactiflorin, galloylpaeoniflorin, paeonin, paeonolide, and paeonol [4,5], most of which are monoterpeneglucosides. Paeoniflorin is most abundant (>90%) among them and accounts for the pharmacological effects observed for the total extract of peony glucosides in both in vitro and in vivo studies. Chi Shao shares similar chemical compounds with Bai Shao but also shows subtle differences from it . Dan Pi has been reported to exhibit antiproliferative, anti-inflammation, anti-infection, antihypertensive, antidiabetic, and neuroprotective activities . The active compounds of P. suffruticosa, such as paeonol and paeoniflorin, have also been previously identified [8,9]. Although all these three traditional Chinese medicine materials from Paeonia plants have paeoniflorin and gallic acid, as well as their derivatives, their different anatomic parts and the compound distributions contribute directly to their distinct pharmacological effects.
A commercial preparation of the total peony glucoside extract was approved by the State Food and Drug Administration of China as a disease-modifying drug for rheumatoid arthritis in 1998. From its chemical structure, we speculate that benzoylpaeoniflorin and galloylpaeoniflorin originate from paeoniflorin with benzoic acid and gallic acid, respectively (Figure S1). Paeoniflorin is a monoterpene glucoside in which the biosynthesis is controlled by the terpene pathways, including the mevalonate (MVA) and 1-deoxy-d-xylulose-5-phosphate/methyl-erythritol-4-phosphate DXP/MEP pathways (Figure 1). Both the MVA and DXP/MEP pathways lead to the synthesis of the universal precursor, isopentenyl-PP. In the MVA pathway, the key biosynthesis enzymes include hydroxymethylglutaryl-CoA synthase (HMGCS; 220.127.116.11), acetyl-CoA C-acetyltransferase (atoB; 18.104.22.168), HMG-CoA reductase (NADPH) (HMGCR; 22.214.171.124), mevalonate kinase (MVK; 126.96.36.199), and phosphomevalonate kinase (PMK; 188.8.131.52). The key biosynthesis enzymes in the DXP/MEP pathway include 1-deoxy-d-xylulose-5-phosphate synthase (DXPS; 184.108.40.206), 1-deoxy-d-xylulose-5-phosphate reductoisomerase (DXR; 220.127.116.117), 2-C-methyl-d-erythritol 4-phosphate cytidylyltransferase (ispD; 18.104.22.168), 4-diphosphocytidyl-2-C-methyl-d-erythritol kinase (ispE; 22.214.171.124), 2-C-methyl-d-erythritol 2,4-cyclodiphosphate synthase (ispF; 126.96.36.199), (E)-4-hydroxy-3-methylbut-2-enyl-diphosphate synthase (HDS; 188.8.131.52), 4-hydroxy-3-methylbut-2-enyl-diphosphate reductase (HDR; 184.108.40.206), and isopentenyl-diphosphate delta-isomerase (IDI; 220.127.116.11). Benzoic acid and gallic acid are also important compounds in aminobenzoate degradation (KO00627) under the catalytic activities of 3-deoxy-7-phosphoheptulonate synthase (aroA; 18.104.22.168), 3-dehydroquinate synthase (aroB; 22.214.171.124), and 3-dehydroquinate dehydratase/shikimate dehydrogenase (aroDE; 126.96.36.199/188.8.131.52).
A number of studies have reported the functional genes in P. lactiflora and P. suffruticosa. For instance, a mitochondrial phosphate transporter was isolated from a subtractive cDNA library from the bursting buds of P. suffruticosa and its expression in response to chilling treatment during the breakage of bud dormancy . Despite the fact that the expression pattern of flavonoid biosynthetic genes in the flower of herbaceous peony was investigated , data on genes related to the active compounds of Paeonia remain elusive.
In this paper, we obtained genes from the biosynthetic pathway of paeoniflorin and gallic acid in the P. lactiflora transcriptome. We also selected the transcriptome databases of three other plants, for comparative purposes, namely, Punica granatum, Rhus radicans, and Coriaria nepalensis, which contain gallic acid but not paeoniflorin (Table S1) [12–16]. To investigate specific genes related to the biosynthesis of paeoniflorin and gallic acid in P. lactiflora as evidence to distinguish Bai Shao and Chi Shao, we not only analyzed phylogenetic relationships of the relevant genes from P. lactiflora, P. suffruticosa, P. granatum, R. radicans, and C. nepalensis, but also their expressions, and contents of the active compounds in different anatomic parts of P. lactiflora.
2.1. Identification of 24 Paeoniflorin and Gallic Acid Biosynthesis-Related Genes in P. lactiflora and Other Related Plants
Using a systematic computational approach, we identified 24 novel paeoniflorin and gallic acid biosynthesis-related genes from the current P. lactiflora transcriptome assembly (Table 1). These genes are members of a group of 17 genes that encode all the enzymes involved in the biosynthesis of paeoniflorin and gallic acid (Figure 1). Only eight genes have full-length cDNAs, and their deduced amino acids showed different lengths, isoelectric points, molecular weights, subcellular localizations, and signal peptides. These genes share highly conserved domains and motifs with the genes in their gene family, respectively (Table 2).
The genes were identified based on sequence homology to known sequences and domains. For instance, PLDHS1 protein was recognized by its conserved domain of DAHP synthetase (class II, IPR002480). PLHMGCSs protein has the conserved active site (IPR000590) of Hydroxymethylglutaryl-coenzyme A synthase. PLACAT and PLDXP contain their conserved domains of Thiolase (IPR002155) Deoxyxylulose-5-phosphate synthase (IPR005477), respectively. We also identified many orthologous genes in other related plants, such as 28 in P. suffruticosa, 23 in P. granatum, 28 in R. radicans, and 38 in C. nepalensis, based on Blast comparison and annotations referred to KEGG and Interpro (Table S5). Not all orthologous genes were found; for instance, E184.108.40.206 was absent in the data from P. granatum.
2.2. Phylogenetic Analysis of Paeoniflorin and Gallic Acid Biosynthesis-Related Orthologs
Phylogenetic analysis shows that the genes we identified are those involved in the biosynthesis of terpenes and aminobenzoate degradation in Arabidopsis thaliana and Oryza sativa, and branches with bootstrap values above 50% were analyzed (Figures 2 and 3; Figures S2 and S3). We also observe significant sequence divergence, as it is related to genetic distance and functional relevance . One example is shown in Figure 2A, where PLDHS HTIP-2010562 and HTIP-2057123 (E220.127.116.11) are functionally diversified as their sequences change. Similarly, PLaroDE HTIP-2007063 and HTIP-2057051 (E18.104.22.168/22.214.171.124) may also be functionally differentiated (Figure 2C).
A pair of orthologous genes in P. lactiflora (PLDXP3, HTIP-2057315) and P. suffruticosa (JI447843) may also be evolved for differentiated functions as compared with the other genes (Figure S2). There are many orthologous genes among P. suffruticosa, P. granatum, R. radicans, and C. nepalensis, which exhibit conserved homology and may share their functions (Figure S3).
2.3. Tissue-Specific Accumulation of Active Compounds in P. lactiflora
To study the tissue-specific accumulation of the shared active compounds of medicinal plant group Paeonia, which include peony lactone glycosides, paeoniflorin, paeonol, benzoylpaeoniflorin, and benzoic acid, in both P. lactiflora and P. suffruticosa, we first determined the contents of these compounds in various plant tissues (leaves, stems, flowers, buds, carpels, fibrous roots, and roots with or without bark) of P. lactiflora (Figure 4). Peony lactone glycosides are the major compounds in leaves, stems, and carpels, but present in almost all tissues in variable contents. Both peony lactone glycosides and benzoylpaeoniflorin are the major compounds in flowers and buds.
As roots are the medicinal organs here, we divided roots into different portions for the analysis. The contents of peony lactone glycosides are higher in fibrous roots and upper roots than in the middle and lower roots and the fact suggests that the content of peony lactone glycosides is growth related. In addition, the contents of paeoniflorin and benzoylpaeoniflorin appeared bark-associated in that their contents are higher in the bark of lower roots than in the lower bark-free roots. Similarly, the contents of paeonol and benzoic acid are higher in the bark of middle and lower roots than in the bark-free middle and lower roots. Furthermore, our correlation analysis showed that the content of benzoic acid is closely related to that of benzoylpaeoniflorin (R = 0.99; Table S6). These results suggest that the accumulation of most compounds coincides with root growth and some are bark-associated.
2.4. Expression of Gallic Acid Biosynthesis-Related Genes in Different Tissues of P. lactiflora
Among the gallic acid biosynthesis-related genes, HTIP-2010562 and HTIP-2057123 (PLDHS, E126.96.36.199) exhibit different transcription profiles in flowers, carpels, bark-free roots, and bark. PLDHS1 (HTIP-2010562) has a higher transcription level than PLDHS2 (HTIP-2057123) in roots and bark, where active compounds accumulate, and this result suggests the functional importance of PLDHS1 in the synthesis of gallic acid. In addition, HTIP-2007063 and HTIP-2057051 (PLaroDE, E188.8.131.52/184.108.40.206) exhibit similar transcription profiles in different tissues. The transcription levels of PLaroDE1 and PLaroDE2 are higher in bark than in bark-free roots. The change in PLDHQS (HTIP-2010607) was only found in bark-free roots, bark of the lower roots and carpel (Figure 5).
2.5. Expression of Paeoniflorin Biosynthesis-Related Genes in Different Tissues of P. lactiflora
In the MVA pathway, the transcription levels of PLHMGCR (HTIP-20156491) and PLACAT are lower in roots than in flowers. However, the transcription level of PLACAT is higher in the bark-free root than in the bark. In addition, PLHMGS2 (HTIP-2056640), PLPMK (HTIP-2056266), and PLMVD1 (HTIP-2044815) exhibit similar transcription profiles that are higher in roots than in flowers (Figure 6). In the MEP/DXP pathway, PLDXR2 (HTIP-2006951), PLIspF (HTIP-2054028), PLHDS (HTIP-2007108), PLIspD (HTIP-2008416), PLHDR (HTIP-2056592), PLCMK (HTIP-2008205), and PLIDI (HTIP-2055407) display similar transcription profiles among all tissues (Figure S4). Some differential expressions were found for the three PLDXPSs, HTIP-2003675, HTIP-2057315, and HTIP-203301 (Figure S5).
2.6. Correlation of Gene Expression and Active Compound Contents
Among the five gallic acid biosynthesis-related genes of shikimate biosynthesis, only HTIP-2057051 and HTIP-2007063 encoding aroDE (E220.127.116.11/18.104.22.168) are closely related to the contents of benzoic acid and benzoylpaeoniflorin, with correlation coefficients higher than 0.8 (Table 3). In the MVA pathway, the transcription levels of PLHMGS1 (HTIP-2054545) and PLPMK (HTIP-2056266) were also deemed closely related to the contents of benzoic acid and benzoylpaeoniflorin, whereas HTIP-2056640 was closely related to the content of paeoniflorin (R = 0.89).
3.1. Preferential Expression of Gene Families in Bark
Historically, the function of P. lactiflora bark has always been a matter of debate. The analysis of the chemistry and genomics of Chi Shao and Bai Shao can help in defining it. Chemical analysis showed that the contents of paeoniflorin and benzoylpaeoniflorin are higher in the lower parts of the bark. The contents of paeoniflorin in the upper and middle parts of the bark were not significantly different from that in roots without bark, which can be attributed to the weight ratio of bark to whole roots. The transcription levels of PLaroDEs (HTIP-2057051 and HTIP-2007063) in shikimate biosynthesis as well as PLMVK (HTIP-2009308), PLPMK (HTIP-2056266), and PLMVD1 (HTIP-2044815) in the mevalonate pathway were higher in bark than in roots without bark. The results of the transcription profiles showed that paeoniflorin and its derivative aromatic amino acid biosynthesis-related genes have higher expressions in bark, suggesting their higher contents therein. Our results from gene expression and chemical analysis showed that paeoniflorin and its aromatic amino acid derivatives were predominant in bark, thus supporting the view that bark is the discriminating factor. In addition, the transcription levels of PLACAT (HTIP-2011414) and PLDXPS1 (HTIP-203301) were higher in roots without bark than in bark, suggesting that other compounds may also have important functions in the pharmacological activity of Bai Shao.
3.2. Variations of Key Genes in Shikimate Biosynthesis and the MVA Pathway Lead to Active Compound Accumulation
Paeoniflorin and its aromatic amino acid derivatives, such as benzoylpaeoniflorin, are medicinally active compounds in P. lactiflora. In addition, the biosynthesis of paeoniflorin and benzoylpaeoniflorin is branched from glycolysis and involved shikimate and terpenoid biosynthesis. To investigate which genes govern the accumulation of paeoniflorin and its derivatives in P. lactiflora, we analyzed the root-associated expression of key genes in shikimate and terpenoidbiosyntheses, couple with the correlation of their transcriptions and active compound contents. In plants, the shikimate pathway leads to the biosynthesis of aromatic amino acids, and the bifunctional aroDE catalyzes the conversion of dehydroquinate into shikimate. Suppressed aroDE expression in tobacco reduces the content of aromatic amino acids and downstream products . In the root of P. lactiflora, HTIP-2057051 and HTIP-2007063 of the aroDE gene family (E22.214.171.124/126.96.36.199) in shikimate biosynthesis are closely related to the accumulation of benzoic acid and benzoylpaeoniflorin. Paeoniflorin is a monoterpene glucoside produced via the mevalonate-independent pathway, as with other monoterpenes . HMG-CoA synthaseof Arabidopsis is an enzyme of the mevalonate pathway involved in the biosynthesis of isoprenoids, such as sterols . An improvement in HMG-CoA synthase has been reported to increase MVA production in Escherichia coli . PMK was found to catalyze a key step in isoprenoid/sterol biosynthesis, converting mevalonate 5-phosphate and ATP to mevalonate 5-diphosphate and ADP . Expression analysis revealed the importance of HMG-CoA synthase, MVK, and other genes in the provision of precursors for rubber biosynthesis . Overexpression of HMGS reportedly up-regulates HMGR, leading to enhanced sterol content and stress tolerance in Arabidopsis . Our results also showed that HTIP-2054545 and HTIP-2056640 (the HMG-CoA synthase gene family, E188.8.131.52) and HTIP-2056266 (the PMK gene family, E184.108.40.206) in the mevalonate pathway are potentially closely related to the accumulation of paeoniflorin and benzoylpaeoniflorin. The content of paeoniflorin was used as a standard to evaluate the chemical quality of P. lactiflora in The Chinese Pharmacopoeia, and increasing the expression of the above-mentioned genes may improve the content of paeoniflorin in the roots and the chemical quality of P. lactiflora.
3.3. Gene and Functional Diversity of Paeoniflorin and Gallic Acid Biosyntheses Pathway
The functional divergence of enzymes is often a result of gene duplication followed by sequence variation, known as neofunctionalization and subfunctionalization [26,27]. Paeoniflorin is unique to Paeonia but aromatic amino acids, such as gallic acid, are abundant in most of plants, e.g., P. lactiflora, P. suffruticosa, P. granatum, R. radicans, and C. nepalensis. Based on phylogenetic analysis, 14 paralogs and orthologs were used to predict the functional diversity of the active compounds. In gallic acid biosynthesis, PLaroDE paralogs could have the same function, and their orthologs were also found in P. suffruticosa, P. granatum, R. radicans, and C. nepalensis. According to the results of gene expression analysis, aroDE orthologs and paralogs had a similarly expression pattern in different tissues and are related to the contents of the aromatic amino acids. In paeoniflorin biosynthesis, PLDXPS paralogs may represent a distinct function based on phylogenetic analysis and showed diverse expressed patterns in different tissues. These findings suggest that the complexity of orthologs and paralogs in paeoniflorin and gallic acid biosynthesis can lead to differences in their functional diversity and chemical compound contents.
4. Experimental Section
4.1. Plant Materials
Fresh leaves, flowers, carpels, and roots of P. lactiflora were collected from the Medicinal Plant Garden of Beijing University of Chinese Medicine (Figure 7). The root trunk was divided into three parts, the upper, the middle, and the lower. Each root part was further divided into root without bark and bark only. Five plants were sampled and authenticated by Chunsheng Liu (Beijing University of Chinese Medicine, Beijing, China).
4.2. Gene Prediction and KEGG Annotation
The current transcriptomic assemblies of P. lactiflora, P. granatum, R. radicans, and C. nepalensis were from the 1 kP Project ( http://www.1kp-project.com) and the cDNA assemblies of P. suffruticosa were retrieved from the public database (GenBank; Available online: http://www.ncbi.nlm.nih.gov). The sequences were annotated based on KEGG  and orthologs of the paeoniflorin and gallic acid biosynthesis-related genes from various plant species were identified by using BLASTx  with an e-value cutoff of 10−5. All retrieved sequences from the Genscan web server ( http://genes.mit.edu/GENSCAN.html) were used for gene prediction . The predicted gene models were further examined and corrected manually by comparison with related genes identified from other plant species.
4.3. Sequence and Phylogenic Analyses
Theoretical isoelectric points and molecular weights were predicted using the Compute pI/MW tool on the ExPASy server ( http://web.expasy.org/compute_pi/) . The localizations of the deduced proteins were predicted on the TargetP 1.1 server ( http://www.cbs.dtu.dk/services/TargetP/) as well as iPSORT and PSORT programs ( http://ipsort.hgc.jp/). Conserved domains were searched against the Pfam protein family database with InterProScan ( http://www.ebi.ac.uk/Tools/pfa/iprscan/) . The conserved amino acids were analyzed by protein alignment using such tools as ClustalW  and checked manually . Phylogenetic analysis was performed by using ClustalW  and MEGA 5.0  for neighbor-joining analysis. The reliability of these tree topologies was evaluated with bootstrapping support of 1000 replicates .
4.4. RNA Extraction and PCR Analysis
Total RNA was extracted from plant tissues in Trizol reagent (Invitrogen, New York, CA, USA) and pretreated with RNase-Free DNase (Promega, Madison, WI, USA) to eliminate genomic DNA contamination. RNA integrity was analyzed on 1% agarose gel. RNA quantity was determined with a NanoDrop 2000C spectrophotometer (Thermo Scientific, Waltham, MA, USA). Total RNA was reverse-transcribed with Reverse Transcriptase MMLV (D6130; Takara, Dalian, China) from flowers, carpels, bark-free roots, and bark. PCRs were performed in triplicate by using SYBR Premix Ex Taq kits (DRR066A; TaKaRa, Dalian, China) and following the manufacturer’s instructions on an ABI 7500 Real-Time PCR System (ABI, Austen, TX, USA). Gene-specific primers (Table S2) were designed using primer designing tools, particularly Primer3 ( http://frodo.wi.mit.edu/primer3/) . Plactin (GenBankJN105299; Available online: http://www.ncbi.nlm.nih.gov) was chosen as an endogenous control in studying gene expressions in various samples of P. lactiflora. Standard deviations were calculated from the three PCR replicates. The specificity of amplification was assessed by melting curve analysis, and the relative abundance of genes was determined based on the comparative Ct method as suggested in ABI 7500 Software v2.0.1 (ABI, Austen, TX, USA).
4.5. Chemical Analysis
The dried samples were separately comminuted with a miller. Each solid sample (40 mesh, 0.50 g) was accurately weighed and extracted with 50 mL of 50% aqueous methanol by ultrasonication for 30 min. The extract was cooled to 25 °C, diluted with 50 mL 50% aqueous methanol, and then filtered with a 0.45-μm Millipore filter membrane (Millipore, MA, USA).
The HPLC system used was an Agilent 1200 LC Series (Agilent Technologies, Palo Alto, CA, USA) consisting of an online vacuum degasser (G1379B), an SL binary pump (G1312B), an autosampler (GB67C), a thermostatic column compartment (G1316B), and a diode-array detector (G1315C) coupled with an analytical workstation. The column configuration was an Agilent TC-C18 column (5 μm, 250 mm × 4.6 mm). The sample injection volume was 10 μL. The detection wavelength was set at 230 nm for analysis with the flow rate at 1.0 mL min−1, and the column temperature was maintained at 25 °C. The mobile phase consisted of deionized water/formic acid (A; 98:2, v/v) and acetonitrile (B). The elution conditions are shown in Table S3. To determine the linearity of the chromatographic techniques, we constructed calibration plots of eight standards on the basis of peak areas (y) using seven concentration solutions (x). All plots were linear in the examined ranges, and linear ranges were shown at different concentrations for the standard compounds (μg/mL). The r value in Table S4 refers to the correlation coefficient of the equation. All standard compounds, which were purchased from the National Institutes for Food and Drug Control, showed good linearity (r > 0.9995) in a relatively wide concentration range.
4.6. Correlation Analysis
Correlation analysis conducted with SPSS 13.0 (IBM Corporation, http://www.ibm.com/software/analytics/spss/) was dependent on the contents of active compounds and the transcript levels of paeoniflorin and gallic acid biosynthesis related genes in the roots of P. lactiflora.
We examine 24 genes and their expressions involved in the biosynthesis of paeoniflorin and its derivatives in Paeonia medicinal materials. We also associated metabolic pathways involved in processing active medicinal compounds to the expressions of their catalytic enzymes. Our study shows that paeoniflorin and its derived aromatic amino acids are predominant in bark.
Acknowledge data from the 1kP project and the project was funded by Natural Science Foundation of China (81001605).
Conflicts of Interest
The authors declare no conflict of interest.
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|Enzyme||Name||Contig No.||Length (bp)|
|Name||Length (aa)||Molecular weight (KD)||Isoelectric point||Subcellular Localization||Signal peptide||Domains (# of domains)|
|DHS1||528||58.7||8.06||Mit.||No||DAHP synthetase, class II(IPR002480)|
|HMGCS1||453||50.6||6.10||Cytosol||No||Hydroxymethylglutaryl-coenzyme A synthase, active site(IPR000590)|
|HMGCS2||465||51.3||5.98||Cytosol||No||Hydroxymethylglutaryl-coenzyme A synthase, active site(IPR000590)|
|DXPS2||714||77.0||6.60||Chl.||No||Deoxyxylulose-5-phosphate synthase (IPR005477)|
|DXPS3||719||78.9||6.17||Cytosol||No||Deoxyxylulose-5-phosphate synthase (IPR005477)|
|IspF||231||25.0||7.89||Chl.||No||2-C-methyl-d-erythritol 2,4-cyclodophosphate synthase (IPR003526)|
|Enzyme||Gene||Peony lactone glycosides||Paeoniflorin||Benzoic acid||Benzoyl paeoniflorin||Paeonol|
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