Next Article in Journal
Antioxidant and Protective Mechanisms against Hypoxia and Hypoglycaemia in Cortical Neurons in Vitro
Next Article in Special Issue
Phosphosite Mapping of HIP-55 Protein in Mammalian Cells
Previous Article in Journal
Evaluation of Osseointegration of Titanium Alloyed Implants Modified by Plasma Polymerization
Article Menu

Export Article

Open AccessCommunication
Int. J. Mol. Sci. 2014, 15(2), 2465-2474; doi:10.3390/ijms15022465

Mass Spectrometry Analysis Coupled with de novo Sequencing Reveals Amino Acid Substitutions in Nucleocapsid Protein from Influenza A Virus

1
Key Laboratory of Cardiovascular Molecular Biology and Regulatory Peptides, Ministry of Health/ Key Laboratory of Molecular Cardiovascular Sciences, Ministry of Education/ Beijing Key Laboratory of Cardiovascular Receptors Research, Institute of Vascular Medicine, Peking University Third Hospital, Beijing 100191,China
2
Key Laboratory of Zoonosis, Ministry of Education, Jilin University, Changchun 130062, Jilin, China
3
Center for Disease Control and Prevention, Changchun 130025, Jilin, China
4
Central Laboratory, Jilin University Second Hospital, Changchun 130041, Jilin, China
*
Author to whom correspondence should be addressed.
Received: 31 December 2013 / Revised: 26 January 2014 / Accepted: 29 January 2014 / Published: 11 February 2014
(This article belongs to the Special Issue Mass Spectrometry Application in Biology)
View Full-Text   |   Download PDF [596 KB, uploaded 19 June 2014]   |  

Abstract

Amino acid substitutions in influenza A virus are the main reasons for both antigenic shift and virulence change, which result from non-synonymous mutations in the viral genome. Nucleocapsid protein (NP), one of the major structural proteins of influenza virus, is responsible for regulation of viral RNA synthesis and replication. In this report we used LC-MS/MS to analyze tryptic digestion of nucleocapsid protein of influenza virus (A/Puerto Rico/8/1934 H1N1), which was isolated and purified by SDS poly-acrylamide gel electrophoresis. Thus, LC-MS/MS analyses, coupled with manual de novo sequencing, allowed the determination of three substituted amino acid residues R452K, T423A and N430T in two tryptic peptides. The obtained results provided experimental evidence that amino acid substitutions resulted from non-synonymous gene mutations could be directly characterized by mass spectrometry in proteins of RNA viruses such as influenza A virus.
Keywords: nucleocapsid protein; influenza virus A virus; amino acid substitution; mass spectrometry nucleocapsid protein; influenza virus A virus; amino acid substitution; mass spectrometry
This is an open access article distributed under the Creative Commons Attribution License (CC BY 3.0).

Supplementary material

Scifeed alert for new publications

Never miss any articles matching your research from any publisher
  • Get alerts for new papers matching your research
  • Find out the new papers from selected authors
  • Updated daily for 49'000+ journals and 6000+ publishers
  • Define your Scifeed now

SciFeed Share & Cite This Article

MDPI and ACS Style

Li, Z.; Sun, W.; Wu, D.; Gao, X.; Sun, N.; Liu, N. Mass Spectrometry Analysis Coupled with de novo Sequencing Reveals Amino Acid Substitutions in Nucleocapsid Protein from Influenza A Virus. Int. J. Mol. Sci. 2014, 15, 2465-2474.

Show more citation formats Show less citations formats

Related Articles

Article Metrics

Article Access Statistics

1

Comments

[Return to top]
Int. J. Mol. Sci. EISSN 1422-0067 Published by MDPI AG, Basel, Switzerland RSS E-Mail Table of Contents Alert
Back to Top