Maize ZmRACK1 Is Involved in the Plant Response to Fungal Phytopathogens
AbstractThe receptor for activated C kinase 1 (RACK1) belongs to a protein subfamily containing a tryptophan-aspartic acid-domain (WD) repeat structure. Compelling evidence indicates that RACK1 can interact with many signal molecules and affect different signal transduction pathways. In this study, we cloned a maize RACK1 gene (ZmRACK1) by RT-PCR. The amino acid sequence of ZmRACK1 had seven WD repeats in which there were typical GH (glycine-histidine) and WD dipeptides. Comparison with OsRACK1 from rice revealed 89% identity at the amino acid level. Expression pattern analysis by RT-PCR showed that ZmRACK1 was expressed in all analyzed tissues of maize and that its transcription in leaves was induced by abscisic acid and jasmonate at a high concentration. Overexpression of ZmRACK1 in maize led to a reduction in symptoms caused by Exserohilum turcicum (Pass.) on maize leaves. The expression levels of the pathogenesis-related protein genes, PR-1 and PR-5, increased 2.5–3 times in transgenic maize, and reactive oxygen species production was more active than in the wild-type. Yeast two-hybrid assays showed that ZmRACK1 could interact with RAC1, RAR1 and SGT1. This study and previous work leads us to believe that ZmRACK1 may form a complex with regulators of plant disease resistance to coordinate maize reactions to pathogens.
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Wang, B.; Yu, J.; Zhu, D.; Chang, Y.; Zhao, Q. Maize ZmRACK1 Is Involved in the Plant Response to Fungal Phytopathogens. Int. J. Mol. Sci. 2014, 15, 9343-9359.
Wang B, Yu J, Zhu D, Chang Y, Zhao Q. Maize ZmRACK1 Is Involved in the Plant Response to Fungal Phytopathogens. International Journal of Molecular Sciences. 2014; 15(6):9343-9359.Chicago/Turabian Style
Wang, Baosheng; Yu, Jingjuan; Zhu, Dengyun; Chang, Yujie; Zhao, Qian. 2014. "Maize ZmRACK1 Is Involved in the Plant Response to Fungal Phytopathogens." Int. J. Mol. Sci. 15, no. 6: 9343-9359.