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Int. J. Mol. Sci. 2014, 15(6), 9481-9496; doi:10.3390/ijms15069481

A Novel VHH Antibody Targeting the B Cell-Activating Factor for B-Cell Lymphoma

1
Department of Lymphoma, Affiliated Hospital of Academy of Military Medical Sciences, Beijing 100071, China
2
Department of Blood Transfusion, Jinan Military General Hospital, Jinan 250031, China
3
Tianjin International Joint Academy of Biotechnology and Medicine, Tianjin Shengfa NabioTech Co., Ltd., Tianjin 300457, China
4
Department of Molecular and Cellular Pharmacology, School of Pharmaceutical Science and Technology, Tianjin University, Tianjin 300072, China
5
Division of Science Popularization, China Science and Technology Exchange Center, Beijing 100045, China
*
Authors to whom correspondence should be addressed.
Received: 21 April 2014 / Revised: 13 May 2014 / Accepted: 14 May 2014 / Published: 28 May 2014
(This article belongs to the Section Molecular Diagnostics)
View Full-Text   |   Download PDF [647 KB, 19 June 2014; original version 19 June 2014]   |  

Abstract

Objective: To construct an immune alpaca phage display library, in order to obtain a single domain anti-BAFF (B cell-activating factor) antibody. Methods: Using phage display technology, we constructed an immune alpaca phage display library, selected anti-BAFF single domain antibodies (sdAbs), cloned three anti-BAFF single-domain antibody genes into expression vector pSJF2, and expressed them efficiently in Escherichia coli. The affinity of different anti-BAFF sdAbs were measured by Bio layer interferometry. The in vitro biological function of three sdAbs was investigated by cell counting kit-8 (CCK-8) assay and a competitive enzyme-linked immunosorbent assay (ELISA). Results: We obtained three anti-BAFF single domain antibodies (anti-BAFF64, anti-BAFF52 and anti-BAFFG3), which were produced in high yield in Escherichia coli and inhibited tumor cell proliferation in vitro. Conclusion: The selected anti-BAFF antibodies could be candidates for B-cell lymphoma therapies.
Keywords: immune VHH phage display library; B cell-activating factor; sdAb immune VHH phage display library; B cell-activating factor; sdAb
This is an open access article distributed under the Creative Commons Attribution License (CC BY 3.0).

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MDPI and ACS Style

Wu, W.; Li, S.; Zhang, W.; Sun, J.; Ren, G.; Dong, Q. A Novel VHH Antibody Targeting the B Cell-Activating Factor for B-Cell Lymphoma. Int. J. Mol. Sci. 2014, 15, 9481-9496.

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