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Int. J. Mol. Sci. 2014, 15(6), 9780-9788; doi:10.3390/ijms15069780

Multiplex Hydrolysis Probe Real-Time PCR for Simultaneous Detection of Hepatitis A Virus and Hepatitis E Virus

Institute for Viral Disease Control and Prevention, Chinese Center for Disease Control and Prevention, No. 155 Changbai Road, Beijing 102206, China
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Author to whom correspondence should be addressed.
Received: 11 February 2014 / Revised: 31 March 2014 / Accepted: 21 April 2014 / Published: 30 May 2014
(This article belongs to the Section Molecular Diagnostics)
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Abstract

Detection of hepatitis viral infections has traditionally relied on the circulating antibody test using the enzyme-linked immunosorbent assay. However, multiplex real-time PCR has been increasingly used for a variety of viral nucleic acid detections and has proven to be superior to traditional methods. Hepatitis A virus (HAV) and hepatitis E virus (HEV) are the major causes of acute hepatitis worldwide; both HAV and HEV infection are a main public health problem. In the present study, a one-step multiplex reverse transcriptase quantitative polymerase chain reaction assay using hydrolysis probes was developed for simultaneously detecting HAV and HEV. This novel detection system proved specific to the target viruses, to be highly sensitive and to be applicable to clinical sera samples, making it useful for rapid, accurate and feasible identification of HAV and HEV.
Keywords: multiplex TaqMan real-time PCR; hepatitis A virus; hepatitis E virus; identification; application multiplex TaqMan real-time PCR; hepatitis A virus; hepatitis E virus; identification; application
This is an open access article distributed under the Creative Commons Attribution License (CC BY 3.0).

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MDPI and ACS Style

Qiu, F.; Cao, J.; Su, Q.; Yi, Y.; Bi, S. Multiplex Hydrolysis Probe Real-Time PCR for Simultaneous Detection of Hepatitis A Virus and Hepatitis E Virus. Int. J. Mol. Sci. 2014, 15, 9780-9788.

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