Modification of Low Molecular Weight Polysaccharides from Tremella Fuciformis and Their Antioxidant Activity in Vitro
Abstract
:1. Introduction
2. Experimental Section
2.1. Materials
2.2 Preparation of LTP
2.3. Modification of LTP
2.4. Assay for Antioxidant Activity
2.4.1. DPPH Radical Scavenging Activity of SLTP, LTP and TP
2.4.2. Hydroxyl Radical Scavenging Activity of SLTP, LTP and TP
2.4.3. Superoxide Anion radical Scavenging Activity of SLTP, LTP and TP
2.5. Statistical Analysis
3. Results and Discussion
3.1. Modification of LTP
3.2. Antioxidant activity
3.2.1. DPPH radical scavenging activity of SLTP, LTP and TP
3.2.2. Hydroxyl radical scavenging activity of SLTP, LTP and TP
3.2.3. Superoxide anion radical scavenging activity of SLTP, LTP and TP
4. Conclusions
NO. | Temperature (°C) | Degree of sulphate (DS) | Water solubility (mg/ml) |
---|---|---|---|
SLTPI | 60 | 0.41±0.03 | 23±4.04 |
SLTPII | 80 | 0.66±0.03 | 29±2.51 |
SLTPIII | 100 | 1.02±0.09 | 41±2.56 |
LTP | 11±2.01 | ||
TP | 3±0.47 |
Concentration | TP | LTP | SLTPI | SLTPII | SLTPIII | Vc | Acontrol |
---|---|---|---|---|---|---|---|
10 μg/ml | 0.486±0.012 | 0.461±0.017 | 0.427±0.006 | 0.407±0.013 | 0.340±0.008 | 0.433±0.008 | 0.553 |
20 μg/ml | 0.464±0.015 | 0.448±0.019 | 0.401±0.007 | 0.388±0.008 | 0.309±0.006 | 0.417±0.007 | |
50 μg/ml | 0.424±0.008 | 0.408±0.011 | 0.364±0.012 | 0.339±0.014 | 0.256±0.009 | 0.372±0.012 |
Concentration | TP | LTP | SLTPI | SLTPII | SLTPIII | Vc | Acontrol |
---|---|---|---|---|---|---|---|
10 μg/ml | 0.928±0.014 | 0.882±0.008 | 0.842±0.012 | 0.734±0.003 | 0.675±0.012 | 0.958±0.010 | 1.057 |
20 μg/ml | 0.808±0.006 | 0.778±0.009 | 0.695±0.007 | 0.572±0.009 | 0.503±0.013 | 0.889±0.006 | |
50 μg/ml | 0.736±0.005 | 0.691±0.003 | 0.591±0.007 | 0.487±0.005 | 0.360±0.006 | 0.844±0.006 |
Concentration | TP | LTP | SLTPI | SLTPII | SLTPIII | Vc | Acontrol |
---|---|---|---|---|---|---|---|
10 μg/ml | 0.588±0.013 | 0.547±0.009 | 0.528±0.013 | 0.503±0.009 | 0.482±0.010 | 0.545±0.009 | 0.758 |
20 μg/ml | 0.564±0.010 | 0.531±0.007 | 0.495±0.008 | 0.476±0.008 | 0.454±0.012 | 0.510±0.007 | |
50 μg/ml | 0.516±0.010 | 0.491±0.006 | 0.469±0.011 | 0.448±0.004 | 0.411±0.010 | 0.453±0.012 |
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Wu, Q.; Zheng, C.; Ning, Z.-X.; Yang, B. Modification of Low Molecular Weight Polysaccharides from Tremella Fuciformis and Their Antioxidant Activity in Vitro. Int. J. Mol. Sci. 2007, 8, 670-679. https://doi.org/10.3390/i8070670
Wu Q, Zheng C, Ning Z-X, Yang B. Modification of Low Molecular Weight Polysaccharides from Tremella Fuciformis and Their Antioxidant Activity in Vitro. International Journal of Molecular Sciences. 2007; 8(7):670-679. https://doi.org/10.3390/i8070670
Chicago/Turabian StyleWu, Qiong, Cheng Zheng, Zheng-Xiang Ning, and Bao Yang. 2007. "Modification of Low Molecular Weight Polysaccharides from Tremella Fuciformis and Their Antioxidant Activity in Vitro" International Journal of Molecular Sciences 8, no. 7: 670-679. https://doi.org/10.3390/i8070670