Isolation and Identification of a Strain of Aspergillus Tubingensis With Deoxynivalenol Biotransformation Capability
Abstract
:1. Introduction
2. Results and Discussion
2.1. Isolation of NJA-1
2.2. Identification of NJA-1
2.3. The effect of transformation
2.4. Screening for DON biotransformation product by NJA-1
3. Experimental Section
3.1. Sampling
3.2. Culture media
3.3. Purification and isolation
3.4. Identification of NJA-1
3.4.1. The observation of conventional morphology and scanning electron microscope
3.4.2. Identification of NJA-1 with PCR
3.5 The effect of DON transformation
3.6 Sscreening for DON biotransformation
4. Conclusions
Acknowledgments
References
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No. of sample | Original concentration (mg/L) | Concentration after 7d cultivation. (mg/L) | Concentration after 14d cultivation. (mg/L) | Transformation efficiency at 14d (%) |
---|---|---|---|---|
1 | 40 | 20.32±0.26 | 3.28±0.02 | 91.8%* |
2 | 2 | 1.2±0.06 | 0.139±0.02 | 93.1%* |
3 | 1 | 0.56±0.16 | 0.035±0.05 | 96.5%* |
4 | 0.4 | 0.18±0.056 | 0.015±0.07 | 96.2%* |
negative control | 0 | 0 | 0 | 0 |
positive control | 0.5 | 0.498±0.003 | 0.497±0.01 | 0.6% |
© 2008 by the authors; licensee Molecular Diversity Preservation International, Basel, Switzerland. This article is an open-access article distributed under the terms and conditions of the Creative Commons Attribution license ( http://creativecommons.org/licenses/by/3.0/). This article is an open-access article distributed under the terms and conditions of the Creative Commons Attribution license ( http://creativecommons.org/licenses/by/3.0/).
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He, C.; Fan, Y.; Liu, G.; Zhang, H. Isolation and Identification of a Strain of Aspergillus Tubingensis With Deoxynivalenol Biotransformation Capability. Int. J. Mol. Sci. 2008, 9, 2366-2375. https://doi.org/10.3390/ijms9122366
He C, Fan Y, Liu G, Zhang H. Isolation and Identification of a Strain of Aspergillus Tubingensis With Deoxynivalenol Biotransformation Capability. International Journal of Molecular Sciences. 2008; 9(12):2366-2375. https://doi.org/10.3390/ijms9122366
Chicago/Turabian StyleHe, Chenghua, Yanhong Fan, Guofang Liu, and Haibin Zhang. 2008. "Isolation and Identification of a Strain of Aspergillus Tubingensis With Deoxynivalenol Biotransformation Capability" International Journal of Molecular Sciences 9, no. 12: 2366-2375. https://doi.org/10.3390/ijms9122366