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N"-[(3Z)-1-Acetyl-5-chloro-2-oxo-1,2-dihydro-3H-indol-3-ylidene]thiocarbonohydrazide

Institute of Chemistry, Faculty of Natural Sciences and Mathematics, Ss. Cyril and Methodius University, Gazi baba bb, 1000 Skopje, Macedonia
*
Author to whom correspondence should be addressed.
Molbank 2013, 2013(2), M798; https://doi.org/10.3390/M798
Submission received: 8 February 2013 / Accepted: 9 April 2013 / Published: 16 April 2013

Abstract

:
A novel synthetic methodology for preparation of thiocarbohydrazone by reacting thiocarbohydrazide with 1-acetyl-5-chloroisatin is described. The title compound was prepared by condensation of thiocarbohydrazide and substituted isatin in aqueous ethanol. The newly synthesized compound was characterized using 1H-NMR, 13C-NMR, FT-IR and mass spectrometry.

Graphical Abstract

1. Introduction

Isatin (indoline-2,3-dione), possessing an indole nucleus with two chemically distinct cyclic carbonyl groups, keto and lactam, has provoked tremendous interest due to its numerous biological and pharmacological activities. The growing importance of substituted isatins in the field of medicinal chemistry as potential chemotherapeutic agents and their implication for pro-drug design have been previously reported [1,2,3,4,5,6,7,8,9].
Particular attention has been paid to the structural perturbations caused by 5-substitution. Antimicrobial activity studies revealed that substitution in the 5th position of isatin with strong electron-donating atoms/groups, such as chlorine, bromine or fluorine, produced more active compounds in a series compared to the parent molecule [10,11,12]. This observation encouraged us to further study the reactions of 5-halo substituted isatin heterocycles with carbohydrazide and thiocarbohydrazide in order to obtain new derivatives [13].
In the last decades several isatin heterocycles with hydrazine moiety have been prepared that exhibited remarkable biological activity including antimicrobial, antitubercular, antiviral, anti-inflammatory, anticonvulsant, antihypertensive, hypoglycemic, cytotoxic, anticancer activity and enzymatic inhibition [14]. The Schiff-bases of the isatin analogs also showed inhibitory activity against eLF2 kinase activator [15], TNF-α, CDK2 [16] and SARS protease [17]. In addition, isatin-β-thiosemicarbazone derivatives possess a range of chemotherapeutical activities [18,19].
Considering the fact that the thiocarbohydrazone group is necessary for antimicrobial activity [20], we have investigated the carbonyl-amine reactions of some isatin derivatives and we have observed that N-ethylisatin-β-thiocarbohydrazone has cytostatic activity towards malignant melanoma cells [9]. Herein we report the synthesis and spectral characterization of the novel derivative N"-[(3Z)-1-acetyl-5-chloro-2-oxo-1,2-dihydro-3H-indol-3-ylidene]thiocarbonohydrazide (4).

2. Results and Discussion

The title compound 4 was obtained via three steps as described in Scheme 1. The first step involved synthesis of isatin derivative 5-chloroindoline-2,3-dione (2) using a modified Sandmeyer methodology [21]. It was prepared with dehydrative cyclization of the precursor 4-chloroisonitrosoacetanilide (1) with concentrated H2SO4 at controlled temperature. Compound 1 was synthesized in very good yield by reaction of the respective aromatic amine 4-chloroaniline (a) with chloral hydrate and hydroxylamine hydrochloride.
In order to hinder the formation of isatin-like centrosymmetric dimers in the carbonyl-amine reaction of 2 with thiocarbohydrazide, the second step was N-substitution of 5-chloroindoline-2,3-dione (2) to obtain the corresponding 1-acetyl derivative (3). 1-Acetyl-5-chloroindoline-2,3-dione (3) was prepared by adapting a literature method [22].
Finally, in the carbonyl-amine condensation of the disubstituted isatin (3) with thiocarbohydrazide (TCH), synthesized according to the Taguchi method [23], 4 was obtained. In order to improve the yield of 4, the effect of the mole ratio of reactants and the amount of solvents was investigated. It was found that the corresponding isatin product was predominantly obtained by using equimolar amounts of 3 and TCH, in ethanol/water (3:1, v/v) as a solvent system.
The structure of the title compound (4) was confirmed using FT-IR, 1H-NMR, 13C-NMR and MSn.
Complex bands dominate in the 3,500–2,800 cm−1 spectral region of N"-[(3Z)-1-acetyl-5-chloro-2-oxo-1,2-dihydro-3H-indol-3-ylidene]thiocarbonohydrazide. The band at around 3,315 cm−1 can be attributed with great certainty to ν(NH). The most prominent bands in the spectrum of the synthesized compound (4) are the ones due to the carbonyl stretchings ν(C=O) of the keto and lactam group in the region around 1,700 cm−1. The absence of carbonyl (C=O) peak at around 1,750 cm−1 characteristic for the keto group in (3) could explain the formation of a thiocarbohydrazone.
The 1H-NMR spectra of (4) displayed three separate singlets at 14.15, 9.54 and 6.62 ppm which can be attributed to C=NN-H, C=NNH(CS)N-H and C=NNH(CS)NH-NH2 from the thiocarbohydrazide moiety, respectively. The assigment of the peak at δ 14.15 as C=NN-H is supported by its location at a lower field (indicative of an intramolecularly hydrogen-bonded proton) [24]. According to the spectral data analysis, the synthesized monothiocarbohydrazone is a Z-geometrical isomer.
The mass spectrum (MS) of (4) was obtained by electrospray ionization in the negative mode and it demonstrated a molecular ion [M−H] at m/z 310 as a base peak in MS1 with the corresponding M+2 peak at m/z 312 due to the 37Cl (Figure 1). The base peak in MS2 was observed at m/z 236 with isotopic peaks at 237 and 238, which results from cleavage of the –CSNHNH2 part of the molecule. Fragments at m/z 208, 194 and 166 with low intensity (1%–2%) were also observed in MS2. Further fragmentation of the ion at m/z 236 in MS3 led to two main fragments at m/z 194 and 166 followed by their corresponding isotopic peaks. The peak at m/z 194 is due to a loss of an acetyl group, and the peak at 166 to a further loss of CO, which occurs in amides as Claisen rearrangement [25].

3. Experimental

3.1. General

Melting points were determined using Koffler apparatus and were uncorrected. C, H elemental analysis was carried out with Coleman Model 33. N elemental analysis was carried out by the Dümas method. Infrared spectra (KBr pellets) were measured on a Perkin-Elmer System 2000 FT-IR. NMR spectra were recorded on Gemini NMR Spectrometer (200 MHz) determined in either DMSO-d6 or CDCl3 as solvent and using tetramethylsilane as internal standard. The mass spectrometer was a G2449A Ion-Trap Mass Spectrometer (Agilent Technologies Inc., Santa Clara, CA, USA) equipped with an electrospray ionisation (ESI) system and controlled by LCMSD software v.6.1. A syringe pump (kdScientific) was used for introducing the sample in the MS with a flow rate of 1.5 mL/h. Nitrogen was used as a nebulizing gas at pressure of 12 psi and the flow was adjusted to 5 L·min−1. The heated capillary and the voltage were maintained at 350 °C and 3.5 kV, respectively. MS data were acquired in the negative ionization mode. The full scan covered the mass range m/z 100–1,000. Collision–induced fragmentation experiments were performed in the ion trap using helium as collision gas, with voltage ramping cycle from 0.3 up to 2 V. Maximum accumulation time of the ion trap and the number of MS repetitions to obtain the MS average spectra were set at 300 ms and 5, respectively.
Preparative flash chromatography was performed using Merck silica gel 60 (230–400 mesh) and thin layer chromatography (TLC) was carried out on aluminum sheets with silica gel with fluorescent indicator (254 nm), obtained from Sigma-Aldrich. Spots were visualized using either UV-lamp at 254 nm or iodine.

3.2. Preparation Procedures

All chemicals used for synthesis and purification were of p.a. grade (Merck). Commercial isatin was recrystallized twice from ethanol. Compounds 1, 2 and 3 are not commercially available and they were prepared by adapting reported methods [2,21,22]. The Sandmeyer methodology was modified as described below. 4-chloroisonitrosoacetanilide (1). The reaction mixture was refluxed for 30 min and the desired product was obtained by recrystallization from ethanol (86%) m.p. 160 °C (lit. [2] rec. EtOAc, 170–172 °C). 5-chloroindoline-2,3-dione (2). 4-Chloroisonitrosoacetanilide (1) was added to a pre-warmed concentrated sulfuric acid (70–75 °C), at such a rate as to maintain the reaction temperature between 85 and 90 °C. After the addition, the reaction mixture was heated at 95–100 °C for 15 min. The red product 2 was obtained via recrystallization from 50% aqueous acetic acid (92%). M.p. 250 °C (lit. [2] rec. EtOAc, 230–232 °C). The spectroscopic data matched those reported in the literature [2].
N"-[(3Z)-1-Acetyl-5-chloro-2-oxo-1,2-dihydro-3H-indol-3-ylidene]thiocarbonohydrazide. A solution of thiocarbohydrazide (0.53 g, 5 mmol) in 10 mL hot water was added in small aliquots to 1-acetyl-5-chloroindoline-2,3-dione (1.12 g, 5 mmol) in 30 mL of ethanol. The mixture was refluxed for five minutes and then was stirred overnight at room temperature. The resulting yellow precipitate was collected by vacuum filtration, washed with ethanol/water solution (3:1, v/v) and air dried. Bright yellow solid was obtained after recrystallization from ethanol in typical yield of 70%.
M.p.: 252–253 °C
FTIR (KBr, cm−1): ν(NH) 3319, 3192, 3174, 3140, ν(CO) 1672, ν(CO) lactam 1605.
1H-NMR (200 MHz, DMSO-d6): δ/ppm 14.15 (s, 1H, NH, C=N-NH-CS), 9.54 (s, 1H, NH CSNHNH2), 6.62 (s, 2H, CSNHNH2), 7.9–7.47 (m, 3H, Ar-H), 2.01 (s, 3H, CH3CO).
13C-NMR (200 MHz, DMSO-d6): δ/ppm 182.87 (C=S), 168.68 (C=O, NCOCH3), 148.64 (C=O, lactam), 143.34 (C=N), 136.38 (CAr), 130.71 (CAr), 130.18 (CAr), 127.59 (CAr), 125.63 (CAr), 125.00 (CAr), 24.15 (NCOCH3).
MS m/z: [M−H] at 310 (100%) represents the molecular ion with formula C11H1035ClN5O2S (minus one H due to the negative ionization mode). The corresponding isotopic peak (for 37Cl) was observed at m/z 312 (26.1%).
MS2 m/z: 236 (100%), 237 (7.5%), 238 (28.0%), 208 (1.5%), 194 (1%), 166 (1.5%).
MS3 m/z: 194 (91.1%), 195 (10.2%), 196 (20.5%), 166 (100%), 167 (11.7%), 168 (22.6%).
Anal. Calcd. for C11H10ClN5O2S (311.75): C, 42.38; H, 3.23; N, 22.46. Found: C, 42.22; H, 3.16; N, 22.25.

4. Conclusions

The isatin derivatives are very useful synthetic intermediates and can function as suitable building blocks for preparation of other biologically active compounds. The N"-[(3Z)-1-acetyl-5-chloro-2-oxo-1,2-dihydro-3H-indol-3-ylidene]thiocarbonohydrazide was synthesized in good yield and its identity was confirmed using FT-IR, 1H-NMR, 13C-NMR and MSn. The biological properties and the crystal structure of the newly synthesized 4 will be thoroughly investigated.

Supplementary materials

Supplementary File 1Supplementary File 2Supplementary File 3

References

  1. Mariappan, P.; Srinivasan, R.; Kumarasamy, K.; Ganesan, S.; Velayutham, T.; Thangavel, S.; Kandasamy, K. Antioxidant and anticancer activities of isatin (1H-indole-2,3-dione), isolated from the flowers of Couroupita guianensis Aubl. Indian J. Med. Res. 2012, 136, 822–826. [Google Scholar]
  2. Islam, M.R.; Mohsin, M. Synthesis of isatin, 5-chloroisatin and their Δ2–1,3,4 oxadiazoline derivatives for comparative cytotoxicity study on brine shrimp. Bangladesh J. Pharmacol. 2007, 2, 7–12. [Google Scholar]
  3. Matesic, L.; Locke, J.M.; Bremner, J.B.; Pyne, S.G.; Skropeta, D.; Ranson, M.; Vine, K.L. N-phenethyl and N-naphthylmethyl isatins and analogues as in vitro cytotoxic agents. Bioorg. Med. Chem. 2008, 16, 3118–3124. [Google Scholar] [CrossRef] [PubMed]
  4. Gao, N.; Kramer, L.; Rahmani, M.; Dent, P.; Grant, S. The three-substituted indolinone cyclin-dependent kinase 2 inhibitor 3-[1-(3H-imidazol-4-yl)-meth-(Z)-ylidene]-5-methoxy-1,3-dihydro-indol-2-one (SU9516) kills human leukemia cells via down-regulation of Mcl-1 through a transcriptional mechanism. Mol. Pharmacol. 2006, 70, 645–655. [Google Scholar] [CrossRef] [PubMed]
  5. Wang, F.; Fang, Y.; Zhu, T.; Zhang, M.; Lin, A.; Gu, Q.; Zhu, W. Seven new prenylated indole diketopiperazine alkaloids from holothurianderived fungus Aspergillus fumigatus. Tetrahedron 2008, 64, 7986–7991. [Google Scholar] [CrossRef]
  6. Lane, M.E.; Yu, B.; Rice, A.; Lipson, K.E.; Liang, C.; Sun, L.; Tang, C.; McMahon, G.; Pestell, R.G.; Wadler, S. A novel cdk2-selective inhibitor, SU9516, induces apoptosis in colon carcinoma cells. Cancer Res. 2001, 61, 6170–6177. [Google Scholar] [PubMed]
  7. Patyna, S.; Laird, A.D.; Mendel, D.B.; O’Farrell, A.M.; Liang, C.; Guan, H.; Vojkovsky, T.; Vasile, S.; Wang, X.; Chen, J.; et al. SU14813: A novel multiple receptor tyrosine kinase inhibitor with potent antiangiogenic and antitumor activity. Mol. Cancer Ther. 2006, 5, 1774–1782. [Google Scholar] [CrossRef] [PubMed]
  8. Li, H.H.; Zheng, X.H.; Tan, J.Z.; Chen, L.L.; Liu, H.; Luo, X.M.; Shen, X.; Lin, L.P.; Chen, K.X.; Ding, J.; et al. Design, synthesis, antitumor evaluations and molecular modeling studies of novel 3,5-substituted indolin-2-one derivatives. Acta Pharmacol. Sin. 2007, 28, 140–152. [Google Scholar] [CrossRef] [PubMed]
  9. Juranić, Z.; Anastasova, F.; Juranić, I.; Stanojković, T.; Radulović, S.; Vuletić, N. Antiproliferative action of isatine-β-thiocarbohydrazone and N-ethylisatine-β-thiocarbohydrazone on human PBMC and on two neoplastic cell lines. J. Exp. Clin. Cancer Res. 1999, 18, 317–324. [Google Scholar] [PubMed]
  10. Bhavesh, R.N.; Kishor, S.P.; Manish, M.J.; Mayur, R.P. Synthesis and antimicrobial activity of some new isatin derivatives. Der Pharma Chemica 2011, 3, 367–372. [Google Scholar]
  11. Madhu; Priyanka, B.; Maharaj; Krishnaveni, J.; Brahmeswari, G.; Sarangapani, M.; Sammaiah, G. Synthesis and antimicrobial activity of some new isatin derivatives. J. Adv. Pharm. Sci. 2011, 1, 10–15. [Google Scholar]
  12. Naumov, P.; Anastasova, F. Experimental and theoretical vibrational stydy of isatin, its 5-(NO2, F, Cl, Br, I, CH3) analogues and the isatinato anion. Spectrochim. Acta A Mol. Biomol. Spectrosc. 2001, 57, 469–481. [Google Scholar] [CrossRef]
  13. Anastasova, F.B. Synthesis of Heterocyclic Azomethynes with Potential Biological Activity. Ph.D. Thesis, Faculty of Science, Sts. Cyril and Methodius University, Skopje, Macedonia, 1997. [Google Scholar]
  14. Pandeya, S.N.; Smitha, S.; Jyoti, M.; Sridhar, S.K. Biological activities of isatin and its derivatives. Acta Pharm. 2005, 55, 27–46. [Google Scholar]
  15. Natarajan, A.; Fan, Y.H.; Chen, H.; Guo, Y.; Iyasere, J.; Harbinski, F.; Christ, W.J.; Aktas, H.; Halperin, J.A. 3,3-Diaryl-1,3-dihydroindol-2-ones as antiproliferatives mediated by translation initiation inhibition. J. Med. Chem. 2004, 47, 1882–1885. [Google Scholar] [CrossRef] [PubMed]
  16. Matheus, M.E.; Violante, F.D.A.; Garden, S.J.; Pinto, A.C.; Fernandes, P.D. Isatins inhibit cyclooxigenase-2 and inducible nitric oxide synthase in a mouse macrophage cell line. Eur. J. Pharmacol. 2007, 556, 200–206. [Google Scholar] [CrossRef] [PubMed]
  17. Zhou, L.; Liu, Y.; Zhang, W.; Wei, P.; Huang, C.; Pei, J.; Yuan, Y.; Lai, L. Isatin compounds as noncovalent SARS coronavirus 3C-like protease inhibitors. J. Med. Chem. 2006, 49, 3440–3443. [Google Scholar] [CrossRef] [PubMed]
  18. Kang, I.J.; Wang, L.W.; Hsu, T.A.; Yueh, A.; Lee, C.C.; Lee, Y.C.; Lee, C.Y.; Chao, Y.S.; Shih, S.R.; Chern, J.H. Isatin-β-thiosemicarbazones as potent herpes simplex virus inhibitors. Bioorg. Med. Chem. Lett. 2011, 21, 1948–1952. [Google Scholar] [CrossRef] [PubMed]
  19. Hall, M.D.; Salam, N.K.; Hellawell, J.L.; Fales, H.M.; Kensler, C.B.; Ludwig, J.A.; Szakács, G.; Hibbs, D.E.; Gottesman, M.M. Synthesis, activity, and pharmacophore development for isatin-beta-thiosemicarbazones with selective activity toward multidrug-resistant cells. J. Med. Chem. 2009, 52, 3191–3204. [Google Scholar] [CrossRef] [PubMed]
  20. Kiran, G.; Maneshwar, T.; Rajeshwar, Y.; Sarangapani, M. Microwave-assisted synthesis, characterization, antimicrobial and antioxidant activity of some new isatin derivatives. J. Chem. 2013, 2013. [Google Scholar] [CrossRef]
  21. Da Silva, J.F.M.; Garden, S.J.; Pinto, A.C. The Chemistry of isatins: A review from 1975 to 1999. J. Braz. Chem. Soc. 2001, 12, 273–324. [Google Scholar] [CrossRef]
  22. Boechat, N.; Kover, W.B.; Bastos, M.M.; Romeiro, N.C.; Silva, A.S.C.; Santos, F.C.; Velverde, A.L.; Azevedo, M.L.G.; Wollinger, W.; Souza, T.M.L.; et al. Design, synthesis, and biological evaluation of new 3-hydroxy-2-oxo-3-trifluoromethylindole as potential HIV-1 reverse transcriptase inhibitors. Med. Chem. Res. 2007, 15, 492–510. [Google Scholar] [CrossRef]
  23. Zhou, J.; Wu, D.; Guo, D. Optimization of the production of thiocarbohydrazide using the Taguchi method. J. Chem. Technol. Biot. 2010, 85, 1402–1406. [Google Scholar] [CrossRef]
  24. Konstantinović, S.S.; Kapor, A.; Radovanović, B.C.; Deak, A. Synthesis, X-ray and antimicrobial activity of isatin-3-phenylhydrzone. Chem. Ind. Chem. Eng. Q. 2008, 14, 27–34. [Google Scholar] [CrossRef]
  25. Wang, H.-Y.; Xu, C.; Zhu, W.; Liu, G.-S.; Guo, Y.-L. Gas phase decarbonylation and cyclization reactions of protonated N-methyl-N-phenylmethacrylamide and its derivatives via an amide Claisen rearrangement. J. Am. Soc. Mass Spectrom. 2012, 23, 2149–2157. [Google Scholar] [CrossRef] [PubMed]
Scheme 1. Synthesis of N"-[(3Z)-1-acetyl-5-chloro-2-oxo-1,2-dihydro-3H-indol-3-ylidene]thiocarbonohydrazide (4).
Scheme 1. Synthesis of N"-[(3Z)-1-acetyl-5-chloro-2-oxo-1,2-dihydro-3H-indol-3-ylidene]thiocarbonohydrazide (4).
Molbank 2013 m798 sch001
Figure 1. ESI MS spectra of N"-[(3Z)-1-acetyl-5-chloro-2-oxo-1,2-dihydro-3H-indol-3-ylidene]thiocarbonohydrazide (4).
Figure 1. ESI MS spectra of N"-[(3Z)-1-acetyl-5-chloro-2-oxo-1,2-dihydro-3H-indol-3-ylidene]thiocarbonohydrazide (4).
Molbank 2013 m798 g001

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MDPI and ACS Style

Ristovska, N.; Anastasova, F.; Stefova, M. N"-[(3Z)-1-Acetyl-5-chloro-2-oxo-1,2-dihydro-3H-indol-3-ylidene]thiocarbonohydrazide. Molbank 2013, 2013, M798. https://doi.org/10.3390/M798

AMA Style

Ristovska N, Anastasova F, Stefova M. N"-[(3Z)-1-Acetyl-5-chloro-2-oxo-1,2-dihydro-3H-indol-3-ylidene]thiocarbonohydrazide. Molbank. 2013; 2013(2):M798. https://doi.org/10.3390/M798

Chicago/Turabian Style

Ristovska, Nataša, Frosa Anastasova, and Marina Stefova. 2013. "N"-[(3Z)-1-Acetyl-5-chloro-2-oxo-1,2-dihydro-3H-indol-3-ylidene]thiocarbonohydrazide" Molbank 2013, no. 2: M798. https://doi.org/10.3390/M798

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