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Short Note

3-Ethyl-2-(ethylimino)-4-methyl-2,3-dihydro-1,3-thiazole-5-carboxylate Ethyl Ester

School of Pharmacy, Health Science Center, Xi’an Jiaotong University, Xi’an, Shaanxi 710061, China
*
Author to whom correspondence should be addressed.
Molbank 2016, 2016(4), M919; https://doi.org/10.3390/M919
Submission received: 2 November 2016 / Revised: 22 November 2016 / Accepted: 22 November 2016 / Published: 29 November 2016
(This article belongs to the Section Organic Synthesis)

Abstract

:
An efficient procedure to obtain the new compound 1a from ethyl acetoacetate (2a), NBS and N,N′-diethylthiourea (4a) was reported. In comparison with the traditional method to synthesize its analogues, this efficient, catalyst-free, and one-pot synthetic method is facile. The work-up procedure is easy and gives the pure target compound under milder reaction conditions in a relatively high yield of 75%.

1. Introduction

In view of the importance of functionalized 2,3-dihydrothiazole derivatives in both the organic synthesis [1] and the biological fields [2], several preparation methods have been developed [3,4,5,6]. The traditional synthetic route to this type of skeleton (1) usually involves two steps from β-keto esters (2), a halogenated reagent such as N-bromosuccinamide (NBS) or N-chlorosuccinamide (NCS), and thiourea or its derivatives (4) (Scheme 1) [7]. This method usually involves the tedious purification procedures of the halogenated intermediates (3a and 3b). In order to obtain diversely substituted 2,3-dihydrothiazole-4-carboxylate esters efficiently, an improved method employed isothiocyanate, primary alkylamine, and 2-chloro-1,3-dicarbonyl compounds as the starting materials [8]. The latter material could be changed into β-nitroacrylates in ionic liquid [9]. The main material isothiocyanates of those methods usually need to be synthesized and purified ahead of the usage, which hampers the wide use of this method. Recently, a method to synthesize 1,3-thiazol-2-imine derivatives from benzoylphenylthioureas and α-bromoketones generated in situ from the reaction of enolizable ketones with 1,1′-(ethane-1,2-diyl)dipyridinium bistribromide (EDPBT, or 1,2-dipyridinium ditribromide-ethane, DPTBE) has been reported [10,11].
The attempt in our research group to acquire the title compound—3-ethyl- 2-ethylimino-4-methyl-2,3-dihydro-1,3-thiazole-5-carboxylate ethyl ester (1a)—using the reported DBTBE method led to the complete recovery of the starting materials. The conventional synthesis of 1a via Scheme 1 involved a tedious work-up with very low overall yield, which could not be used efficiently.
As part of our recent studies on the new biological heterocyclic compounds [12,13], we now report a successful simple procedure to synthesize 1a from ethyl acetoacetate (2a), NBS, and N,N′-diethylthiourea (4a). While 1a shares a similar structure with the reported analogues [8], it was still reported for the first time by our research group in this paper.
Comparing with the traditional method of synthesizing its analogues, this efficient, catalyst-free, and one-pot synthetic method is facile; the work-up procedure is easy and gives the pure target compound under milder reaction conditions with a relatively high yield of 75%. This method offers an alternative way to provide 2,3-diethyl-2,3-dihydro-1,3-thiazole (1a) instead of the traditional two-step method from disubstituted thioureas and β-keto ester derivatives (Scheme 2).
The methods developed in this paper will enrich the limited arsenal for efficiently constructing various functionalized 2,3-dihydro-1,3-thiazoles, which would be a benefit for further exploration of their potent unknown biological activities. In addition, this method is expected to be useful for the expedient synthesis of a variety of heterocyclic compounds with a thiazole core in more complex structures other than the simple substituted 1a.

2. Experimental Section

Melting points were taken on an X-4 digital melting point apparatus (Shanghai Yice Apparatus & Equipments Co., Ltd, Shanghai, China) and are uncorrected. Elemental analyses were performed on a Carlo-Erba 1106 elemental analyzer (Thermo Scientific, Waltham, MA, USA). IR spectra were recorded on a Nicolet FT-IR 360 spectrophotometer (Thermo Scientific, Waltham, MA, USA). 1H-NMR and 13C-NMR spectra were determined on a Bruker AM-400 (400 MHz) spectrometer (Brucker Company, Fällanden, Switzerland) with tetramethylsilane (TMS) as an internal standard. Chemical shifts are reported in δ. Mass spectra were measured on a HP5988A instrument (Hewlett-Packard, Palo Alto, CA, USA) by direct inlet at 70 eV. All materials were obtained from commercial suppliers and used as received.

Ethyl 3-ethyl-2-(ethylamino)-2,3-dihydro-4-methylthiazole-5-carboxylate (1a)

To a mixture of ethyl acetoacetate (2, 6.50 g, 50.0 mmol) in water (30.0 mL) and tetrahydrofuran (THF) (28.0 mL) cooled to −5–0°C was added NBS (10.7 g, 60.0 mmol, 1.20 equiv.). The reaction mixture was stirred at room temperature for 1.0 h, and thin-layer chromatography (TLC, petroleum ether-ethyl acetate v/v = 2:1) showed the disappearance of 2. N,N′-diethylthiourea (4a, 6.60 g, 50.0 mmol, 1.00 equiv.) was added, and the reaction mixture was heated to 95 °C for 19 h. After cooling to room temperature, the reaction mixture was filtered to remove an insoluble red precipitate, and then NH3.H2O (8.0 mL) was added to the filtrate. The resulting yellow solid was collected via filtration under the reduced pressure. The filter cake was washed with water (100 mL × 3), recrystallized from ethyl acetate, then dried to give the target compound as the yellow cubic crystals (1a, 9.10 g, 75%), mp. 63–64 °C, 1H-NMR (CDCl3, 400 MHz): δ 1.23–1.29 (m, 6H, NCH2CH3× 2), 1.33 (t, 3H, OCH2CH3), 2.57 (s, 3H, thiazole-4-CH3), 3.17 (q, 2H, 3-N-CH2CH3), 3.89 (q, 2H, 2-N-CH2CH3), 4.26 (q, 2H, OCH2CH3); 13C-NMR (100 MHz, CDCl3): δ 12.72 (thiazole-4-CH3), 13.32 (thiazole-3-N-CH2CH3), 14.38 (-OCH2CH3),15.36 (=NCH2CH3), 38.82 (thiazole-3-NCH2CH3), 48.91 (=NCH2CH3), 60.41 (OCH2CH3), 98.62 (thiazole-5-C),147.16 (thiazole-2-C), 155.74 (thiazole-4-C), 162.38 (O=C). MS: m/z 243 (M + H+). Elemental Analysis. Calcd for C11H18N2O2S: C, 54.52; H, 7.49; N, 11.56. Found: C, 54.41; H, 7.36; N, 11.42.

3. Conclusions

To summarize, a new compound with the name of 2-ethyl-2-ethylimino-4-methyl-2,3-dihydro- 1,3-thiazole-5-carboxylate ethyl ester was synthesized efficiently via an improved one-pot reaction with a relatively high yield. This method might be useful in further synthesis of the compounds with the similar structure skeleton.

Supplementary Materials

The following are available online at www.mdpi.com/1422-8599/2016/4/M919. The original spectra of the title compound were provided in Figures S1 and S2.
Supplementary File 1Supplementary File 2Supplementary File 3Supplementary File 4

Acknowledgments

The authors are grateful to the Scientific Research Foundation for the Returned Overseas Chinese Scholars, State Education Ministry, P. R. China (2011) and the Shaanxi Province Science and Technology Research and Development Program of China, International Cooperation (2013KW31-04) for the financial supports.

Author Contributions

Ge Meng was responsible for designing the synthesis method and strategy. Mei Wang synthesized the title compound for the first time using the new one-pot reaction. Ya-Nan Cheng repeated this procedure two years later. Kai Chen repeated the NMR spectral analysis of this compound. Jing Tong has synthesized this compound for the further biological evaluations. Jie-He Zhang has done the biological activity screening test on this compound.

Conflicts of Interest

The authors declare no conflict of interest.

References

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Scheme 1. The reported general synthesis route of 2,3-dihydrothiazole derivatives.
Scheme 1. The reported general synthesis route of 2,3-dihydrothiazole derivatives.
Molbank 2016 m919 sch001
Scheme 2. The efficient one-pot synthesis of the title compound (1a).
Scheme 2. The efficient one-pot synthesis of the title compound (1a).
Molbank 2016 m919 sch002

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MDPI and ACS Style

Meng, G.; Wang, M.; Cheng, Y.-N.; Chen, K.; Tong, J.; Zhang, J.-H. 3-Ethyl-2-(ethylimino)-4-methyl-2,3-dihydro-1,3-thiazole-5-carboxylate Ethyl Ester. Molbank 2016, 2016, M919. https://doi.org/10.3390/M919

AMA Style

Meng G, Wang M, Cheng Y-N, Chen K, Tong J, Zhang J-H. 3-Ethyl-2-(ethylimino)-4-methyl-2,3-dihydro-1,3-thiazole-5-carboxylate Ethyl Ester. Molbank. 2016; 2016(4):M919. https://doi.org/10.3390/M919

Chicago/Turabian Style

Meng, Ge, Mei Wang, Ya-Nan Cheng, Kai Chen, Jing Tong, and Jie-He Zhang. 2016. "3-Ethyl-2-(ethylimino)-4-methyl-2,3-dihydro-1,3-thiazole-5-carboxylate Ethyl Ester" Molbank 2016, no. 4: M919. https://doi.org/10.3390/M919

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