Next Article in Journal
A Hierarchical Communication Architecture for Oceanic Surveillance Applications
Next Article in Special Issue
CdTe and CdSe Quantum Dots Cytotoxicity: A Comparative Study on Microorganisms
Previous Article in Journal
On the Biomimetic Design of Agile-Robot Legs
Previous Article in Special Issue
Overview of Stabilizing Ligands for Biocompatible Quantum Dot Nanocrystals
Article Menu

Export Article

Open AccessArticle
Sensors 2011, 11(12), 11335-11342; https://doi.org/10.3390/s111211335

Time-Resolved Fluorescence Immunoassay for C-Reactive Protein Using Colloidal Semiconducting Nanoparticles

1
Laboratory of Biophysics, University of Turku, 20520 Turku, Finland
2
Department of Physics, Tampere University of Technology, 33101 Tampere, Finland
3
Turku University of Applied Sciences, 20520 Turku, Finland
4
Fachbereich Physik and Wissenschaftliches Zentrum für Materialwissenschaften, Philipps Universität Marburg., 35037 Marburg, Germany
*
Author to whom correspondence should be addressed.
Received: 24 August 2011 / Revised: 18 October 2011 / Accepted: 14 November 2011 / Published: 28 November 2011
(This article belongs to the Special Issue Sensing with Quantum Dots)
View Full-Text   |   Download PDF [207 KB, uploaded 21 June 2014]

Abstract

Besides the typical short-lived fluorescence with decay times in the nanosecond range, colloidal II/VI semiconductor nanoparticles dispersed in buffer also possess a long-lived fluorescence component with decay times in the microsecond range. Here, the signal intensity of the long-lived luminescence at microsecond range is shown to increase 1,000-fold for CdTe nanoparticles in PBS buffer. This long-lived fluorescence can be conveniently employed for time-gated fluorescence detection, which allows for improved signal-to-noise ratio and thus the use of low concentrations of nanoparticles. The detection principle is demonstrated with a time-resolved fluorescence immunoassay for the detection of C-reactive protein (CRP) using CdSe-ZnS nanoparticles and green light excitation. View Full-Text
Keywords: colloidal nanoparticles; quantum dots; time-gated fluorescence; sensing; bioaffinity assays colloidal nanoparticles; quantum dots; time-gated fluorescence; sensing; bioaffinity assays
This is an open access article distributed under the Creative Commons Attribution License (CC BY 3.0).
SciFeed

Share & Cite This Article

MDPI and ACS Style

Härmä, H.; Toivonen, J.; Soini, J.T.; Hänninen, P.; Parak, W.J. Time-Resolved Fluorescence Immunoassay for C-Reactive Protein Using Colloidal Semiconducting Nanoparticles. Sensors 2011, 11, 11335-11342.

Show more citation formats Show less citations formats

Related Articles

Article Metrics

Article Access Statistics

1

Comments

[Return to top]
Sensors EISSN 1424-8220 Published by MDPI AG, Basel, Switzerland RSS E-Mail Table of Contents Alert
Back to Top