Immunoanalysis Methods for the Detection of Dioxins and Related Chemicals
AbstractWith the development of biotechnology, approaches based on antibodies, such as enzyme-linked immunosorbent assay (ELISA), active aryl hydrocarbon immunoassay (Ah-I) and other multi-analyte immunoassays, have been utilized as alternatives to the conventional techniques based on gas chromatography and mass spectroscopy for the analysis of dioxin and dioxin-like compounds in environmental and biological samples. These screening methods have been verified as rapid, simple and cost-effective. This paper provides an overview on the development and application of antibody-based approaches, such as ELISA, Ah-I, and multi-analyte immunoassays, covering the sample extraction and cleanup, antigen design, antibody preparation and immunoanalysis. However, in order to meet the requirements for on-site fast detection and relative quantification of dioxins in the environment, further optimization is needed to make these immuno-analytical methods more sensitive and easy to use. View Full-Text
Scifeed alert for new publicationsNever miss any articles matching your research from any publisher
- Get alerts for new papers matching your research
- Find out the new papers from selected authors
- Updated daily for 49'000+ journals and 6000+ publishers
- Define your Scifeed now
Tian, W.; Xie, H.Q.; Fu, H.; Pei, X.; Zhao, B. Immunoanalysis Methods for the Detection of Dioxins and Related Chemicals. Sensors 2012, 12, 16710-16731.
Tian W, Xie HQ, Fu H, Pei X, Zhao B. Immunoanalysis Methods for the Detection of Dioxins and Related Chemicals. Sensors. 2012; 12(12):16710-16731.Chicago/Turabian Style
Tian, Wenjing; Xie, Heidi Q.; Fu, Hualing; Pei, Xinhui; Zhao, Bin. 2012. "Immunoanalysis Methods for the Detection of Dioxins and Related Chemicals." Sensors 12, no. 12: 16710-16731.