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Open AccessCommunication
Sensors 2012, 12(12), 17320-17329; doi:10.3390/s121217320

An Ultrasensitive Chemiluminescence Biosensor for Carcinoembryonic Antigen Based on Autocatalytic Enlargement of Immunogold Nanoprobes

Department of Chemistry, Capital Normal University, Beijing 100048, China
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Author to whom correspondence should be addressed.
Received: 19 October 2012 / Revised: 27 November 2012 / Accepted: 5 December 2012 / Published: 13 December 2012
(This article belongs to the Section Biosensors)
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Abstract

A sensitive flow injection chemiluminescence assay for carcinoembryonic antigen (CEA) detection based on signal amplification with gold nanoparticles (NPs) is reported in the present work. The sandwich system of CEA/anti-CEA/goat-anti-mouse IgG functionalized Au nanoparticles was used as the sensing platform. In order to improve detection sensitivity, a further gold enlargement step was developed based on the autocatalytic Au deposition of gold nanoprobes via the reduction of AuCl4 to Au0 on their surface in the presence of NH2OH·HCl. AuCl4, which is a soluble product of gold nanoprobes, served as an analyte in the CL reaction for the indirect measurement of CEA. Under optimized conditions, the CL intensity of the system was linearly related to the logarithm of CEA concentration in the range of 100 pg∙mL−1 to 1,000 ng∙mL−1, with a detection limit of 20 pg∙mL−1. View Full-Text
Keywords: carcinoembryonic antigen; flow injection chemiluminescence; gold nanoparticle; enlargement carcinoembryonic antigen; flow injection chemiluminescence; gold nanoparticle; enlargement
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This is an open access article distributed under the Creative Commons Attribution License (CC BY 3.0).

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Hao, M.; Ma, Z. An Ultrasensitive Chemiluminescence Biosensor for Carcinoembryonic Antigen Based on Autocatalytic Enlargement of Immunogold Nanoprobes. Sensors 2012, 12, 17320-17329.

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