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Sensors 2012, 12(3), 2969-2982; doi:10.3390/s120302969
Article

An Organic/Inorganic Hybrid Membrane as a Solid “Turn-On” Fluorescent Chemosensor for Coenzyme A (CoA), Cysteine (Cys), and Glutathione (GSH) in Aqueous Media

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Departamento de Química, Facultad de Ciencias, Universidad de Burgos, Plaza de Misael Bañuelos s/n, E-09001 Burgos, Spain
* Author to whom correspondence should be addressed.
Received: 17 January 2012 / Revised: 23 February 2012 / Accepted: 23 February 2012 / Published: 2 March 2012
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Abstract

The preparation of a fluorogenic sensory material for the detection of biomolecules is described. Strategic functionalisation and copolymerisation of a water insoluble organic sensory molecule with hydrophilic comonomers yielded a crosslinked, water-swellable, easy-to-manipulate solid system for water ‘‘dip-in’’ fluorogenic coenzyme A, cysteine, and glutathione detection by means of host-guest interactions. The sensory material was a membrane with gel-like behaviour, which exhibits a change in fluorescence behaviour upon swelling with a water solution of the target molecules. The membrane follows a “turn-on” pattern, which permits the titration of the abovementioned biomolecules. In this way, the water insoluble sensing motif can be exploited in aqueous media. The sensory motif within the membrane is a chemically anchored piperazinedione-derivative with a weakly bound Hg(II). The response is caused by the displacement of the cation from the membrane due to a stronger complexation with the biomolecules, thus releasing the fluorescent sensory moieties within the membrane.
Keywords: sensory materials; chemosensor; fluorogenic sensor; biomolecules; sensing biomolecules sensory materials; chemosensor; fluorogenic sensor; biomolecules; sensing biomolecules
This is an open access article distributed under the Creative Commons Attribution License (CC BY 3.0).

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Vallejos, S.; Estévez, P.; Ibeas, S.; García, F.C.; Serna, F.; García, J.M. An Organic/Inorganic Hybrid Membrane as a Solid “Turn-On” Fluorescent Chemosensor for Coenzyme A (CoA), Cysteine (Cys), and Glutathione (GSH) in Aqueous Media. Sensors 2012, 12, 2969-2982.

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