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Sensors 2012, 12(5), 6176-6185; doi:10.3390/s120506176
Article

luxS Mutant Regulation: Quorum Sensing Impairment or Methylation Disorder?

1,†
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1,†
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1,2
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1
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1
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1
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1,* , 1
 and
1,*
1 Department of Endodontics, Ninth People’s Hospital, Shanghai Key Laboratory of Stomatology, School of Medicine, Shanghai Jiao Tong University, No. 639 Zhizaoju Road, Shanghai 200011, China 2 Department of Endodontics, The Affiliated Stomatology Hospital of Tongji University, School of Stomatology, Tongji University, Shanghai 200072, China These authors contributed equally to this work.
* Authors to whom correspondence should be addressed.
Received: 19 March 2012 / Revised: 12 April 2012 / Accepted: 20 April 2012 / Published: 10 May 2012
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Abstract

AI-2–mediated quorum sensing has been identified in various bacteria, including both Gram-negative and Gram-positive species, and numerous phenotypes have been reported to be regulated by this mechanism, using the luxS-mutant strain. But the AI-2 production process confused this regulatory function; some considered this regulation as the result of a metabolic change, which refers to an important metabolic cycle named activated methyl cycle (AMC), caused by luxS-mutant simultaneously with the defect of AI-2. Herein we hypothesized that the quorum sensing system—not the metabolic aspect—is responsible for such a regulatory function. In this study, we constructed plasmids infused with sahH and induced protein expression in the luxS-mutant strain to make the quorum-sensing system and metabolic system independent. The biofilm-related genes were investigated by real-time polymerase chain reaction (PCR), and the results demonstrated that the quorum-sensing completed strain restored the gene expression of the defective strain, but the metabolically completed one did not. This evidence supported our hypothesis that the autoinducer-2-mediated, quorum-sensing system, not the AMC, was responsible for luxS mutant regulation.
Keywords: quorum sensing; AI-2; luxS; sahH quorum sensing; AI-2; luxS; sahH
This is an open access article distributed under the Creative Commons Attribution License (CC BY 3.0).
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Wang, Q.; He, Z.; Hu, Y.; Jiang, Y.; Ma, R.; Tang, Z.; Liang, J.; Liu, Z.; Huang, Z. luxS Mutant Regulation: Quorum Sensing Impairment or Methylation Disorder? Sensors 2012, 12, 6176-6185.

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