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Sensors 2013, 13(1), 165-174; doi:10.3390/s130100165

Measurement of Urinary N-Telopeptides and Serum C-Telopeptides from Type I Collagen Using a Lateral Flow-Based Immunoassay

1
Department of Orthopaedic Surgery, Seoul National University Bundang Hospital, Kyungki 463-707, Korea
2
Korea Electronics Technology Institute, Yatap-dong, Bundang-gu, Sungnam, Kyungki 463-816, Korea
3
IM Electronics, Maetan-dong, Suwon, Kyungki 443-370, Korea
These authors contributed equally to this work.
*
Author to whom correspondence should be addressed.
Received: 5 November 2012 / Revised: 17 December 2012 / Accepted: 18 December 2012 / Published: 24 December 2012
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Abstract

Measuring bone turnover markers could detect early stages of osteoporosis and early responses to anti-osteoporotic treatments. Currently, commonly used bone turnover markers, N-telopeptides (NTx) and C-telopeptides (CTx), are measured using ELISA tests, which demands time and increases cost. Bone turnover markers need to be measured more easily for general use. Lateral flow-based immunoassay would be an appropriate method for this context. This study was performed to investigate the precision of a newly developed lateral flow-based immunoassay for measuring the urinary NTx and serum CTx, and their correlations with ELISA measurements. Urine NTx and serum CTx concentrations were determined by photoscan of newly developed strips, using a lateral flow-based immunoassay for 36 subjects (mean age 66.2 years, SD 7.5 years; four males and 32 females). Repeated measurement of urinary NTx and serum CTx were performed three times, using this technology for a precision test. The correlation of the lateral flow-based immunoassay with the ELISA measurements was analyzed. Precision of the newly developed lateral flow based immunoassay was 0.974 (ICC, 95% confidence interval, 0.955 to 0.986) and 0.995 (ICC, 95% confidence interval, 0.991 to 0.997) for urinary NTx and serum CTx, respectively. The correlation of lateral flow based immunoassay with ELISA was 0.913 for urinary NTx and 0.872 for serum CTx. These results suggest that measuring the urinary NTx and serum CTx, using a lateral flow-based immunoassay, is a relevant method for point-of-care testing and screening of bone resorption markers. View Full-Text
Keywords: osteoporosis; urine N-telopeptide; serum C-telopeptide; lateral flow immunoassay; bone turnover marker osteoporosis; urine N-telopeptide; serum C-telopeptide; lateral flow immunoassay; bone turnover marker
This is an open access article distributed under the Creative Commons Attribution License (CC BY 3.0).

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MDPI and ACS Style

Lee, K.M.; Lee, M.H.; Chung, C.Y.; Seong, W.K.; Lee, S.D.; Park, M.S. Measurement of Urinary N-Telopeptides and Serum C-Telopeptides from Type I Collagen Using a Lateral Flow-Based Immunoassay. Sensors 2013, 13, 165-174.

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