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Sensors 2013, 13(4), 5109-5116; doi:10.3390/s130405109
Article

Performance Improvement of the One-Dot Lateral Flow Immunoassay for Aflatoxin B1 by Using a Smartphone-Based Reading System

,
*  and
Post-Harvest & Food Engineering Division, Department of Agricultural Engineering, National Academy of Agricultural Sciences, Suwon 441707, Korea
* Author to whom correspondence should be addressed.
Received: 6 February 2013 / Revised: 25 March 2013 / Accepted: 10 April 2013 / Published: 18 April 2013
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Abstract

This study was conducted to develop a simple, rapid, and accurate lateral flow immunoassay (LFIA) detection method for point-of-care diagnosis. The one-dot LFIA for aflatoxin B1 (AFB1) was based on the modified competitive binding format using competition between AFB1 and colloidal gold-AFB1-BSA conjugate for antibody binding sites in the test zone. A Smartphone-based reading system consisting of a Samsung Galaxy S2 Smartphone, a LFIA reader, and a Smartphone application for the image acquisition and data analysis. The detection limit of one-dot LFIA for AFB1 is 5 μg/kg. This method provided semi-quantitative analysis of AFB1 samples in the range of 5 to 1,000 μg/kg. Using combination of the one-dot LFIA and the Smartphone-based reading system, it is possible to conduct a more fast and accurate point-of-care diagnosis.
Keywords: lateral flow immunoassay; aflatoxin B1; Smartphone; point-of-care lateral flow immunoassay; aflatoxin B1; Smartphone; point-of-care
This is an open access article distributed under the Creative Commons Attribution License (CC BY 3.0).
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Lee, S.; Kim, G.; Moon, J. Performance Improvement of the One-Dot Lateral Flow Immunoassay for Aflatoxin B1 by Using a Smartphone-Based Reading System. Sensors 2013, 13, 5109-5116.

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