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Sensors 2007, 7(10), 2484-2491; doi:10.3390/s7102484

D-galactose/D-glucose-binding Protein from Escherichia coli as Probe for a Non-consuming Glucose Implantable Fluorescence Biosensor

1
Laboratory for Molecular Sensing, IBP, CNR, Via Pietro Castellino, 111 80131 Naples, Italy
2
Department of Information Engineering, Seconda Università di Napoli, Italy
*
Author to whom correspondence should be addressed.
Received: 3 October 2007 / Accepted: 21 October 2007 / Published: 24 October 2007
(This article belongs to the Special Issue Optical Biosensors)
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Abstract

D-Galactose/D-glucose-binding protein from E. coli (GGBP) is a monomer thatbinds glucose with high affinity. The protein structure of GGBP is organized in twoprincipal domains linked by a hinge region that form the sugar-binding site. In this workwe show that the mutant form of GGBP at the amino acid position 182 can be utilized as aprobe for the development of a non-consuming analyte fluorescence biosensor to monitorthe glucose level in diabetes health care. View Full-Text
Keywords: Biosensor; Optical sensing; Glucose; Diabetes; Fluorescence; Proteins Biosensor; Optical sensing; Glucose; Diabetes; Fluorescence; Proteins
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Scognamiglio, V.; Aurilia, V.; Cennamo, N.; Ringhieri, P.; Iozzino, L.; Tartaglia, M.; Staiano, M.; Ruggiero, G.; Orlando, P.; Labella, T.; Zeni, L.; Vitale, A.; D’Auria, S. D-galactose/D-glucose-binding Protein from Escherichia coli as Probe for a Non-consuming Glucose Implantable Fluorescence Biosensor. Sensors 2007, 7, 2484-2491.

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