Next Article in Journal
Use of a Dynamic Enclosure Approach to Test the Accuracy of the NDIR Sensor: Evaluation Based on the CO2 Equilibration Pattern
Next Article in Special Issue
Enzyme-Linked Electrochemical Detection of PCR-Amplified Nucleotide Sequences Using Disposable Screen-Printed Sensors. Applications in Gene Expression Monitoring
Previous Article in Journal
Determination of Primary Spectral Bands for Remote Sensing of Aquatic Environments
Previous Article in Special Issue
Gold Nanoparticles With Special Shapes: Controlled Synthesis, Surface-enhanced Raman Scattering, and The Application in Biodetection
Article Menu

Export Article

Open AccessReview
Sensors 2007, 7(12), 3442-3458; doi:10.3390/s7123442

An Overview of Label-free Electrochemical Protein Sensors

School of Materials Science, Japan Advanced Institute of Science and Technology (JAIST),1-1 Asahidai, Nomi City, Ishikawa 923-1292, Japan
Dept. of Chemistry, University of Western Ontario, 1151 Richmond Street, London, Ontario, N6A 5B7, Canada
Nanobiotechnology and Biodevice Laboratory, Dept. of Applied Physics, Graduate School of Engineering, Osaka University, 8-1 Mihogaoka, Ibaraki, Osaka 567-0047, Japan
Author to whom correspondence should be addressed.
Received: 4 December 2007 / Accepted: 18 December 2007 / Published: 20 December 2007
View Full-Text   |   Download PDF [666 KB, uploaded 21 June 2014]   |  


Electrochemical-based protein sensors offer sensitivity, selectivity and reliabilityat a low cost, making them very attractive tools for protein detection. Although the sensorsuse a broad range of different chemistries, they all depend on the solid electrode surface,interactions with the target protein and the molecular recognition layer. Traditionally, redoxenzymes have provided the molecular recognition elements from which target proteins haveinteracted with. This necessitates that the redox-active enzymes couple with electrodesurfaces and usually requires the participation of added diffusional components, or assemblyof the enzymes in functional chemical matrices. These complications, among many others,have seen a trend towards non-enzymatic-based electrochemical protein sensors. Severalelectrochemical detection approaches have been exploited. Basically, these have fallen intotwo categories: labeled and label-free detection systems. The former rely on a redox-activesignal from a reporter molecule or a label, which changes upon the interaction of the targetprotein. In this review, we discuss the label-free electrochemical detection of proteins,paying particular emphasis to those that exploit intrinsic redox-active amino acids.
Keywords: label-free; electrochemical; sensor; protein; amino acids; biosensor; nanoparticles. label-free; electrochemical; sensor; protein; amino acids; biosensor; nanoparticles.
This is an open access article distributed under the Creative Commons Attribution License (CC BY 3.0).

Scifeed alert for new publications

Never miss any articles matching your research from any publisher
  • Get alerts for new papers matching your research
  • Find out the new papers from selected authors
  • Updated daily for 49'000+ journals and 6000+ publishers
  • Define your Scifeed now

SciFeed Share & Cite This Article

MDPI and ACS Style

Vestergaard, M.; Kerman, K.; Tamiya, E. An Overview of Label-free Electrochemical Protein Sensors. Sensors 2007, 7, 3442-3458.

Show more citation formats Show less citations formats

Related Articles

Article Metrics

Article Access Statistics



[Return to top]
Sensors EISSN 1424-8220 Published by MDPI AG, Basel, Switzerland RSS E-Mail Table of Contents Alert
Back to Top