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Sensors 2008, 8(2), 1198-1211; doi:10.3390/s8021198

Dependence of Impedance of Embedded Single Cells on Cellular Behaviour

1,* , 2,* , 2 and 1
1 Biohybrid Systems, Fraunhofer Institute for Biomedical Engineering, Ensheimer Str. 48 66386 St. Ingbert Germany 2 Nanobioengineering Research Laboratory, Bioengineering Institute of Catalonia, Barcelona Science Park, Josep Samitier 1-5, 08028 Barcelona, Spain 3 Electronics Department, University of Barcelona, Martí i Franquès 1, Planta 2 08028 Barcelona, Spain
* Authors to whom correspondence should be addressed.
Received: 31 January 2008 / Accepted: 20 February 2007 / Published: 21 February 2008
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Non-invasive single cell analyses are increasingly required for the medicaldiagnostics of test substances or the development of drugs and therapies on the single celllevel. For the non-invasive characterisation of cells, impedance spectroscopy whichprovides the frequency dependent electrical properties has been used. Recently,microfludic systems have been investigated to manipulate the single cells and tocharacterise the electrical properties of embedded cells. In this article, the impedance ofpartially embedded single cells dependent on the cellular behaviour was investigated byusing the microcapillary. An analytical equation was derived to relate the impedance ofembedded cells with respect to the morphological and physiological change ofextracellular interface. The capillary system with impedance measurement showed afeasibility to monitor the impedance change of embedded single cells caused bymorphological and physiological change of cell during the addition of DMSO. By fittingthe derived equation to the measured impedance of cell embedded at different negativepressure levels, it was able to extrapolate the equivalent gap and gap conductivity betweenthe cell and capillary wall representing the cellular behaviour.
Keywords: electrochemical impedance spectroscopy; microcapillary; single cell analysis. electrochemical impedance spectroscopy; microcapillary; single cell analysis.
This is an open access article distributed under the Creative Commons Attribution License (CC BY 3.0).

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Cho, S.; Castellarnau, M.; Samitier, J.; Thielecke, H. Dependence of Impedance of Embedded Single Cells on Cellular Behaviour. Sensors 2008, 8, 1198-1211.

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