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Sensors 2009, 9(1), 386-403; doi:10.3390/s90100386

Human NK Cell Up-regulation of CD69, HLA-DR, Interferon γ Secretion and Cytotoxic Activity by Plasmacytoid Dendritic Cells is Regulated through Overlapping but Different Pathways

Department of Immunology, Imperial College, Chelsea & Westminster Hospital, London, UK
Department of Immunobiology, King’s College London, Guys Hospital, London, UK
Author to whom correspondence should be addressed.
Received: 9 October 2008 / Revised: 24 December 2008 / Accepted: 7 January 2009 / Published: 9 January 2009
(This article belongs to the Special Issue Dendritic Sensors: From Dendrimer Molecules to Dendritic Cells)
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Human plasmacytoid dendritic cells secrete high levels of IFNa and are thus implicated in the activation of NK cells. Activated NK cells are characterised by the up-regulation of CD69 and MHC class II DR expression, secretion of IFN g and enhanced cytotoxicity. We show that pDC mediate these processes by different mechanisms, some of which overlap. Human NK cells were analysed after co-culture with immature or CpG-matured blood pDC or with supernatant from these cells. Maximal CD69 expression by NK cells was mediated by supernatant from mature pDC and did not require pDC contact. Up-regulation was due in part to IFNa but also to factors in IFNa negative supernatant from immature DC. HLA-DR expression was independent of secreted molecules but required contact with immature or mature DC. Enhanced NK cytotoxicity, measured by killing of K562 targets and expression of CD107a, was mediated by multiple factors including type I IFN, supernatant from immature pDC cultures and contact with immature or mature pDC. These factors act cumulatively to enhance cytotoxcity. Thus different parameters of pDC mediated NK cell activation are regulated by distinct pathways. View Full-Text
Keywords: dendritic cells; NK cells; cytokines; cytotoxicity dendritic cells; NK cells; cytokines; cytotoxicity

This is an open access article distributed under the Creative Commons Attribution License (CC BY 3.0).

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MDPI and ACS Style

Benlahrech, A.; Donaghy, H.; Rozis, G.; Goodier, M.; Klavinskis, L.; Gotch, F.; Patterson, S. Human NK Cell Up-regulation of CD69, HLA-DR, Interferon γ Secretion and Cytotoxic Activity by Plasmacytoid Dendritic Cells is Regulated through Overlapping but Different Pathways. Sensors 2009, 9, 386-403.

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