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Article

4-Methylenesterols from a Sponge Theonella swinhoei

by
Jheng-Kun Guo
1,2,
Ching-Ying Chiang
3,
Mei-Chin Lu
1,4,
Wen-Been Chang
1,2,* and
Jui-Hsin Su
1,4,5,*
1
National Museum of Marine Biology & Aquarium, Pingtung 944, Taiwan
2
Graduate Institute of Marine Biodiversity and Evolutionary Biology, National Dong Hwa University, Pingtung 944, Taiwan
3
Center of General Studies, National Kaohsiung Marine University, Kaohsiung 811, Taiwan
4
Graduate Institute of Marine Biotechnology, National Dong Hwa University, Pingtung 944, Taiwan
5
Asia-Pacific Ocean Research Center, National Sun Yat-sen University, Kaohsiung 804, Taiwan
*
Authors to whom correspondence should be addressed.
Mar. Drugs 2012, 10(7), 1536-1544; https://doi.org/10.3390/md10071536
Submission received: 11 June 2012 / Revised: 28 June 2012 / Accepted: 11 July 2012 / Published: 19 July 2012
Browse Figures
Versions Notes

Abstract

:
Three new 4-methylenesterols, theonellasterol K (1), acetyltheonellasterol (2) and acetyldehydroconicasterol (3), along with two known sterols, theonellasterol (4) and theonellasterone (5), were isolated from the sponge Theonella swinhoei. The structures of these compounds were elucidated on the basis of their spectroscopic data and comparison of the NMR data with those of known analogues. Compound 1 exhibited significant cytotoxic activity against HCT-116, K562 and Molt 4 cancer cell lines.

Graphical Abstract

1. Introduction

In recent years, marine sponges have emerged as one of the most prolific sources for discovery of novel secondary metabolites [1,2,3]. Some of these have been found to possess several kinds of biological activities, such as cytotoxic [4,5], antimicrobial [6], and antiviral properties [7]. 4-Methylenesterols were first reported in 1981 as being obtained from two marine sponges, Theonella conica and Theonella swinhoei, from the Red Sea [8]. Marine sponges of the genus Theonella are now known to be a rich source of novel 4-methylene sterols [8,9,10,11,12,13,14,15,16,17,18,19,20,21]. Some of these sterols display a variety of biological activities, and pharmacological evaluation has shown them to be modulators of two well-known nuclear receptors, FXR and PXR [16,17,18,19,20,21], and cytotoxic activity [9]. Furthermore, a recently published report indicates that theonellasterol (4) is a highly selective FXR antagonist that protects against cholestatic liver injury [21]. Research into the pharmacological properties of this class of natural products is of particular interest. Our investigation of the chemical constituents of the sponge Theonella swinhoei (Figure 1) yielded three new 4-methylene sterols, theonellasterol K (1), acetyltheonellasterol (2) and acetyldehydroconicasterol (3), along with two known sterols, theonellasterol (4) [8] and theonellasterone (5) [10] (Chart 1). The structures of 1–5 were established by detailed spectroscopic analysis, including extensive examination of 2D NMR (1H-1H COSY, HMQC and HMBC) correlations. The cytotoxicity of metabolites 1–5 against human colorectal carcinoma (DLD-1), human hormone-dependent breast cancer (T-47D), human colon adenocarcinoma (HCT-116), human breast adenocarcinoma (MCF-7 and MDA-MB-231), human chronic myelogenous leukemia (K562) and human T lymphoblast, acute lymphoblastic leukemia (Molt 4) was studied in order to discover bioactive compounds.
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Figure 1. Sponge Theonella swinhoei.
Figure 1. Sponge Theonella swinhoei.
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Chart 1. Structures of metabolites 1–5.
Chart 1. Structures of metabolites 1–5.
Marinedrugs 10 01536 g004

2. Results and Discussion

The EtOAc extract of the freeze-dried specimen was fractionated by silica gel column chromatography and the eluted fractions were further separated utilizing normal phase HPLC to yield metabolites 1–5. The new compounds were given the trivial names theonellasterol K (1), acetyltheonellasterol (2) and acetyldehydroconicasterol (3). The known compounds were identified as theonellasterol (4) and theonellasterone (5).
Table
Table 1. 1H and 13C NMR data for 1–3.
Table 1. 1H and 13C NMR data for 1–3.
123
δH ( J in Hz) aδC (mult.) bδH ( J in Hz) aδC (mult.) bδH ( J in Hz) aδC (mult.) b
11.39 m; 1.82 m37.2 (CH2)1.38 m; 1.80 m36.5 (CH2)1.38 m; 1.79 m36.5 (CH2)
21.42 m; 1.98 m 32.7 (CH2)1.50 m; 1.94 m 29.6 (CH2)1.52 m; 1.94 m 29.6 (CH2)
34.01 dd (11.0, 5.0)73.2 (CH)5.15 dd (12.0, 5.0)74.8 (CH)5.15 dd (12.0, 5.0)74.8 (CH)
4 151.9 (C) 148.0 (C) 148.0 (C)
51.97 m45.1 (CH)1.89 d (13.0)49.5 (CH)1.90 m49.5 (CH)
62.07 m; 2.14 m25.1 (CH2)1.38 m; 1.84 m27.0 (CH2)1.40 m; 1.84 m27.0 (CH2)
75.68 d (5.5)125.0 (CH)2.25 m25.8 (CH2)2.25 m25.8 (CH2)
8 135.5 (C) 125.5 (C) 125.6 (C)
92.17 m45.4 (CH)1.80 m49.1 (CH)1.80 m49.1 (CH)
10 37.2 (C) 39.8 (C) 39.8 (C)
111.47 m; 1.64 m20.8 (CH2)1.48 m; 1.64 m20.4 (CH2)1.48 m; 1.64 m20.4 (CH2)
121.62 m; 1.82 m30.5 (CH2)1.14 m; 1.96 m37.3 (CH2)1.15 m; 1.96 m37.3 (CH2)
13 47.3 (C) 42.7 (C) 42.8 (C)
14 98.3 (C) 143.1 (C) 143.0 (C)
151.34 m; 26.9 (CH2)1.75 m; 29.2 (CH2)1.77 m; 29.2 (CH2)
2.06 m 2.47 dd (13.5, 1.0) 2.47 d (14.0)
161.62 m; 2.08 m24.8 (CH2)1.36 m; 1.60 m24.5 (CH2)1.36 m; 1.59 m24.5 (CH2)
171.85 m50.9 (CH)1.16 m56.7 (CH)1.17 m56.7 (CH)
180.74 s17.0 (CH3)0.84 s18.2 (CH3)0.85 s18.2 (CH3)
190.71 s13.6 (CH3)0.62 s13.1 (CH3)0.62 s13.1 (CH3)
201.37 m36.3 (CH)1.46 m34.9 (CH)1.50 m34.4 (CH)
210.89 d (7.0)19.0 (CH3)0.95 d (6.5)19.2 (CH3)0.96 d (6.5)19.1 (CH3)
221.00 m; 1.40 m34.0 (CH2)1.06 m; 1.41 m33.7 (CH2)1.24 m; 1.60 m34.4 (CH2)
231.04 m; 1.34 m26.7 (CH2)1.04 m; 1.32 m26.2 (CH2)1.90 m; 2.08 m30.8 (CH2)
240.94 m46.0 (CH)0.92 m46.1 (CH) 156.9 (C)
251.68 m29.0 (CH)1.68 m28.9 (CH)2.24 m33.8 (CH)
260.81 d (7.0)18.9 (CH3)0.82 d (8.5)19.0 (CH3)1.03 d (7.0)21.9 (CH3)
270.83 d (7.0)19.6 (CH3)0.83 d (7.0)19.5 (CH3)1.04 d (7.5)22.0 (CH3)
281.14 m; 1.32 m23.0 (CH2)1.16 m; 1.32 m23.0 (CH2)4.67 s; 4.73 s105.9 (CH2)
290.86 t (7.5)12.3 (CH3)0.86 t (7.5)12.3 (CH3)4.60 s; 4.89 s103.7 (CH2)
304.74 s; 5.18 s103.6 (CH2)4.60 s; 4.89 s103.7 (CH2)
3-OAc 2.13 s 21.2 (CH3)2.13 s 21.2 (CH3)
170.2 (C)170.2 (C)
14-OOH6.79 br s
a 500 MHz in CDCl3; b 125 MHz in CDCl3; c J values (Hz) are given in parentheses; d Numbers of attached protons were deduced by DEPT experiments.
Theonellasterol K (1) was obtained as a white amorphous solid. The HRESIMS spectrum of 1 exhibited a pseudomolecular ion peak at m/z 481.3659 [M + Na]+, which established a molecular formula of C30H50O3, implying six degrees of unsaturation. IR absorptions were observed at 3362 and 3251 cm−1, suggesting the presence of hydroxy groups in 1. The structure of this compound was deduced from its 13C NMR and DEPT spectroscopic data (Table 1), which showed that the compound has 30 carbons, including six methyls, ten sp3 methylenes, one sp2 methylene, seven sp3 methines (including one oxymethine), one sp2 methine, three sp3 quaternary carbons and two sp2 quaternary carbons. From the 1H NMR spectrum of 1, resonances of one olefinic methine proton (δ 5.68, d, J = 5.5 Hz), two olefinic methylene protons (δ 5.18 and 4.74, each s) and one oxygenated methine (δ 4.01, dd, J = 11.0, 5.0 Hz) were observed. Moreover, the 1H NMR spectrum revealed the presence of one hydroperoxy proton resonating as a broad singlet at δH 6.79. The planar structure and all of the 1H and 13C chemical shifts of 1 were elucidated by 2D NMR spectroscopic analysis, in particular 1H-1H COSY and HMBC experiments (Figure 2). From the 1H-1H COSY correlations, it was found that one ring-juncture methine proton H-5 (δ 1.97) and one oxymethine proton H-3 (δ 4.01) exhibited allylic correlations with the exomethylene protons at C-30 (δH 4.74 and 5.18). In addition, 1H-1H COSY spectral analysis established five partial structures of consecutive proton spin systems (Figure 2). Further analysis of the HMBC correlations was employed successfully to establish the gross structure of 1 (Figure 2). Thus, 1 was found to possess two double bonds at C-7/C-8 and C-4/C-30, one hydroxy group at C-3 and one hydroperoxy group at C-14.
Marinedrugs 10 01536 g002 1024
Figure 2. Selected 1H-1H COSY (▬) and HMBC (→) correlations of 1.
Figure 2. Selected 1H-1H COSY (▬) and HMBC (→) correlations of 1.
Marinedrugs 10 01536 g002
The relative configuration of 1, elucidated mainly from the NOESY spectrum, was compatible with that of 1 ascertained using molecular mechanics calculations (MM2), which suggested the most stable conformations, as shown in Figure 3. In the NOESY spectrum of 1, the NOE correlations between one of the methylene protons at C-11 (δH 1.47) and both methyls (H3-18 and H3-19); H3-18 and H-20 as well as between H3-18, H3-19 and H-20 indicated that these protons adapt a β-orientation. H-5 was found to interact with H-3, but not with H3-19, revealing the β orientation of the hydroxy group at C-3. Moreover, the α-orientation of 14-OOH was further confirmed by the lower field chemical shift of H-17 (δH 1.85, m). Furthermore, the chemical shifts of the side chain from C-20 to C-29 in 1 were nearly identical to those of 4 and 5. Thus, the structure of steroid 1 was established. After determining the structure of 1, we discovered that its molecular framework has been obtained as a known 4-methylenesterol conicasterol H, which was isolated previously from sponge Theonella swinhoei [18].
Marinedrugs 10 01536 g003 1024
Figure 3. Computer-generated model of 1 using MM2 force field calculations and selected NOE correlations from C-1–C-21 of 1.
Figure 3. Computer-generated model of 1 using MM2 force field calculations and selected NOE correlations from C-1–C-21 of 1.
Marinedrugs 10 01536 g003
Acetyltheonellasterol (2) was isolated as a white powder with the molecular formula C32H52O2, which possesses seven degrees of unsaturation, as indicated by HRESIMS (m/z 491.3862, [M + Na]+) and NMR spectroscopic data (Table 1). By comparison of the NMR data of 2 with those of 4, it was found that the 1H and 13C NMR data of 2 were very similar to those of 4, with the difference that 2 contains one more acetyl group relative to 4. The chemical shift of H-3 in 4 (δH 4.01) was shifted downfield (δH 5.15) in 2, suggesting that 2 is the 3-acetyl derivative of 4. We observed further that acetylation of 4 gave a product which was found to be identical to 2 by comparison of the physical and spectroscopic data. Thus, compound 2 was established as the 3-acetyl derivative of 4.
The new metabolite acetyldehydroconicasterol (3) was obtained as a white powder and possessed the molecular formula C31H48O2, as established from the HRESIMS and NMR data, implying eight degrees of unsaturation. Both the 1H and 13C NMR signals of 3 were found to be very closely related to those of compound 2, suggesting a very similar steroidal skeleton. By comparison of the NMR data of 3 with those of 2 (Table 1), it was found that an ethyl protons signal [δH 0.86 t, J = 7.5 Hz (H3-29); 1.16 m and 1.32 m (H2-28)] in 2 was replaced by two exomethylene proton signals (δH 4.73 and 4.67, each s) in 3. The only difference observed was that the 24-ethyl side chain of 2 was replaced by two exomethylene proton signals in 3. This was further confirmed by the HMBC correlations from H2-28 to C-23, C-24, and C-25. On the basis of the above analysis, the structure of 3 was established.
Finally, we used a 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide (MTT) assay to examine the cytotoxic activities of compounds 1–5 against DLD-1, T47D, HCT-116, MCF-7, MDA-MB-231, K562 and Molt 4 cancer cells. Cells were treated with different concentrations of 1–5 for 72 h. The results showed that compound 1, the most potent of compounds 1–5, exhibited cytotoxicity against DLD-1, T47D, HCT-116, MDA-MB-231, K562 and Molt 4, with IC50s of 12.9, 12.0, 6.3, 11.5, 11.4, 4.3, and 6.3 μg/mL, respectively. Furthermore, compound 2 exhibited weak cytotoxic activity against K562 and Molt 4 cancer cell lines (the IC50 values were 13.7 and 17.8 μg/mL for K562 and Molt 4, respectively). The other tested compounds were not cytotoxic (IC50 > 20 μg/mL) towards the above seven cancer cell lines (Table 2).
Table
Table 2. Cytotoxicity (IC50 μg/mL) of compounds 1–5 a.
Table 2. Cytotoxicity (IC50 μg/mL) of compounds 1–5 a.
Cell Lines
DLD-1T-47DHCT-116MCF-7MDA-MB-231K562Molt 4
112.912.06.311.511.44.36.3
2– c– c– c– c– c13.717.8
Doxorubicin b0.420.28 0.89 2.2 1.30.140.009
a Compounds 3–5 was inactive against all seven cell lines (IC50 > 20 μg/mL); b Clinical anticancer drug used as a positive control; c NA, not active at 20 μg/mL.

3. Experimental Section

3.1. General Experimental Procedures

Optical rotation values were measured with a Jasco P-1010 digital polarimeter. IR spectra were recorded on a Varian Digilab FTS 1000 Fourier transform infrared spectrophotometer. The NMR spectra were recorded on a Varian Mercury Plus 400 FT-NMR (or Varian Unity INOVA 500 FT-NMR) instrument at 400 MHz (or 500 MHz) for 1H-NMR and 100 MHz (or 125 MHz) for 13C-NMR, respectively, in CDCl3. ESIMS were obtained with a Bruker APEX II mass spectrometer. Gravity column chromatography was performed on silica gel (230–400 mesh, Merck). TLC was carried out on precoated Kieselgel 60 F254 (0.2 mm, Merck) and spots were visualized by spraying with 10% H2SO4 solution followed by heating. High-performance liquid chromatography (HPLC) was performed using a system comprised of a Hitachi L-7100 pump and a Rheodyne 7725 injection port. A preparative normal phase column (250 × 21.2 mm, 5 μm) was used for HPLC.

3.2. Animal Material

The specimen of Theonella swinhoei was collected by scuba divers at a depth of 15–20 m from coral reefs off the coast of Pingtung, Taiwan. A voucher specimen was deposited in the National Museum of Marine Biology and Aquarium, Taiwan (specimen no. 2011SP-2). Taxonomic identification was performed by Prof. Wen-Been Chang of the National Museum of Marine Biology & Aquarium, Pingtung, Taiwan.

3.3. Extraction and Separation

The sponge Theonella swinhoei (2.2 kg fresh wt) stored frozen and then freeze dried. The freeze-dried material (590 g) was minced and extracted exhaustively with EtOAc (5 × 2 L). The EtOAc extract was evaporated to yield a residue (11.9 g), which was subjected to open column chromatography on silica gel eluting with n-hexane (H)-EtOAc (E) gradient and EtOAc (E)-acetone (A) gradient, to give 12 fractions: Fr-1 (eluted by H-E 100:1), Fr-2 (eluted by H-E 50:1), Fr-3 (eluted by H-E 30:1), Fr-4 (eluted by H-E 20:1), Fr-5 (eluted by H-E 10:1), Fr-6 (eluted by H-E 8:1), Fr-7 (eluted by H-E 5:1), Fr-8 (eluted by H-E 3:1), Fr-9 (eluted by H-E 1:1), Fr-10 (eluted by EtOAc), Fr-11 (eluted by E-A 1:1) and Fr-12 (eluted by acetone). Fraction 2 (250 mg), was subjected to normal phase HPLC (Hibar 250 × 21.2 mm, Supelco, silica gel 60, 5 μm), using n-hexane–EtOAc (25:1) as eluent, to afford four subfractions (A1–A4). Subfraction A1 (50 mg) was separated by normal phase HPLC using n-hexane–EtOAc (25:1) to afford 2 (25.5 mg, 0.21% dry wt of extract) and 3 (1.8 mg, 0.015% dry wt of extract). Subfraction A3 (120 mg) was also purified by normal phase HPLC using n-hexane–EtOAc (15:1) to afford 5 (55 mg, 0.46% dry wt of extract). Fraction 5 (2.5 g), was further separated by silica gel open column chromatography with gradient elution (n-hexane–EtOAc, 10:1 to 5:1) to afford 4 (1.2 g, 10.1% dry wt of extract). Fraction 7 (250 mg), was further separated by normal phase HPLC (n-hexane–EtOAc, 5:1) to yield six subfractions (B1–B6). Subfraction B3 (30 mg) was separated by normal phase HPLC using n-hexane–acetone (6:1) to afford 1 (12.5 mg, 0.11% dry wt of extract).
Theonellasterol K (1): white powder; [α]24D = +82 (c 0.2, CHCl3); IR (neat) νmax 3362, 3251, 2956, 2870, 1644, 1451 and 1376 cm−1; 1H and 13C NMR data, see Table 1; ESIMS m/z 481 [50, (M + Na)+]; HRESIMS m/z 481.3659 (calcd. for C30H50O3Na, 481.3657).
Acetyltheonellasterol (2): white powder; [α]24D = −3 (c 1.0, CHCl3); IR (neat) νmax 2957, 1744, 1644, and 1373 cm−1; 1H and 13C NMR data, see Table 1; ESIMS m/z 491 [80, (M + Na)+]; HRESIMS m/z 491.3862 (calcd. for C32H52O2Na, 491.3865).
Acetyldehydroconicasterol (3): white powder; [α]24D = −5 (c 0.1, CHCl3); IR (neat) νmax 2927, 1742, and 1376 cm−1; 1H and 13C NMR data, see Table 1; ESIMS m/z 475 [100, (M + Na)+]; HRESIMS m/z 475.3555 (calcd. for C31H48O2Na, 475.3552).
Acetylation of 4: A solution of 4 (10.0 mg) in pyridine (0.2 mL) was mixed with Ac2O (0.2 mL), and the mixture was stirred at rt for 24 h. After evaporation of excess reagent, the residue was subjected to column chromatography over Si gel using n-hexane–EtOAc (7:1) to yield 2 (10.5 mg, 95%). The specific rotation [[α]24D = −3 (c 0.5, CHCl3)] was in full agreement with that of the natural product 2.

3.4. Cytotoxicity Testing

Cell lines were purchased from the American Type Culture Collection (ATCC). Cytotoxicity assays of compounds 1–5 were performed using the MTT [3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide] colorimetric method [22,23].

3.5. Molecular Mechanics Calculations

Implementation of the MM2 force filed in Chem3D Pro software [24] was used to calculate the molecular models.

4. Conclusions

A series of new 4-methylenesterols were isolated from the sponge Theonella swinhoei [8,9,10,11,12,13,14,15,16,17,18,19,20,21]. Our continued investigation of the chemical constituents of sponge T. swinhoei has again led to the isolation of three new 4-methylenesterols (1–3) and two known 4-methylenesterols (4 and 5). Compound 1 exhibited significant cytotoxicity against HCT-116, K562 and Molt 4 cells, and moderate to weak cytotoxicity against DLD-1, MCF-7, and MDA-MB-231 cells. In addition, compound 2 exhibited weak cytotoxicity toward K562 and Molt 4 cell lines. However, the new compound 3 and the other known compounds had no significant activity. According to our research, metabolite 1 exhibited significant cancer cells inhibitory activity. This result suggests that 1 should be subjected to further biomedical investigation.

Acknowledgements

This work was supported by grants from the Ministry of Education (00C030205), the National Museum of Marine Biology & Aquarium and the National Science Council (NSC 101-2325-B-291-001), Taiwan, awarded to J.-H. Su.

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  • Samples Availability: Available from the authors.

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    MDPI and ACS Style

    Guo, J.-K.; Chiang, C.-Y.; Lu, M.-C.; Chang, W.-B.; Su, J.-H. 4-Methylenesterols from a Sponge Theonella swinhoei. Mar. Drugs 2012, 10, 1536-1544. https://doi.org/10.3390/md10071536

    AMA Style

    Guo J-K, Chiang C-Y, Lu M-C, Chang W-B, Su J-H. 4-Methylenesterols from a Sponge Theonella swinhoei. Marine Drugs. 2012; 10(7):1536-1544. https://doi.org/10.3390/md10071536

    Chicago/Turabian Style

    Guo, Jheng-Kun, Ching-Ying Chiang, Mei-Chin Lu, Wen-Been Chang, and Jui-Hsin Su. 2012. "4-Methylenesterols from a Sponge Theonella swinhoei" Marine Drugs 10, no. 7: 1536-1544. https://doi.org/10.3390/md10071536

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