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Mar. Drugs 2014, 12(1), 1-16; doi:10.3390/md12010001

Investigating Diet as the Source of Tetrodotoxin in Pleurobranchaea maculata

Department of Biological Sciences, University of Waikato, Private Bag 3105, Hamilton 3240, New Zealand
Cawthron Institute, Nelson 7042, New Zealand
Department of Chemistry, Otago University, Dunedin 9054, New Zealand
Author to whom correspondence should be addressed.
Received: 11 October 2013 / Revised: 18 November 2013 / Accepted: 2 December 2013 / Published: 24 December 2013
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The origin of tetrodotoxin (TTX) is highly debated; researchers have postulated either an endogenous or exogenous source with the host accumulating TTX symbiotically or via food chain transmission. The aim of this study was to determine whether the grey side-gilled sea slug (Pleurobranchaea maculata) could obtain TTX from a dietary source, and to attempt to identify this source through environmental surveys. Eighteen non-toxic P. maculata were maintained in aquariums and twelve were fed a TTX-containing diet. Three P. maculata were harvested after 1 h, 24 h, 17 days and 39 days and TTX concentrations in their stomach, gonad, mantle and remaining tissue/fluids determined using liquid chromatography-mass spectrometry. Tetrodotoxin was detected in all organs/tissue after 1 h with an average uptake of 32%. This decreased throughout the experiment (21%, 15% and 9%, respectively). Benthic surveys at sites with dense populations of toxic P. maculata detected very low or no TTX in other organisms. This study demonstrates that P. maculata can accumulate TTX through their diet. However, based on the absence of an identifiable TTX source in the environment, in concert with the extremely high TTX concentrations and short life spans of P. maculata, it is unlikely to be the sole TTX source for this species.
Keywords: diet; Pleurobranchaea maculata; tetrodotoxin diet; Pleurobranchaea maculata; tetrodotoxin

This is an open access article distributed under the Creative Commons Attribution License (CC BY 3.0).

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MDPI and ACS Style

Khor, S.; Wood, S.A.; Salvitti, L.; Taylor, D.I.; Adamson, J.; McNabb, P.; Cary, S.C. Investigating Diet as the Source of Tetrodotoxin in Pleurobranchaea maculata. Mar. Drugs 2014, 12, 1-16.

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