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Mar. Drugs 2017, 15(10), 306; doi:10.3390/md15100306

Production of Fish Protein Hydrolysates from Scyliorhinus canicula Discards with Antihypertensive and Antioxidant Activities by Enzymatic Hydrolysis and Mathematical Optimization Using Response Surface Methodology

1
Grupo de Reciclado y Valorización de Materiales Residuales (REVAL), Instituto de Investigacións Mariñas (IIM-CSIC), r/Eduardo Cabello, 6, Vigo-36208 Galicia, Spain
2
Grupo de Bioquímica de Alimentos, Instituto de Investigacións Mariñas (IIM-CSIC), r/Eduardo Cabello, 6, Vigo-36208 Galicia, Spain
3
Instituto Nacional de Investigación y Desarrollo Pesquero (INIDEP), Paseo Victoria Ocampo N°1 Escollera Norte, C.C.175 - 7600 Mar del Plata, Argentina
4
Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET), Rivadavia 1917, 1033 Buenos Aires, Argentina
5
Departamento de Química Analítica y Alimentaria, Facultade de Ciencias de Ourense, Universidade de Vigo, Campus As Lagoas s/n, 32004 Ourense, Spain
*
Author to whom correspondence should be addressed.
Received: 25 August 2017 / Revised: 19 September 2017 / Accepted: 4 October 2017 / Published: 10 October 2017
(This article belongs to the Special Issue Bioconversion of Marine Resources)
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Abstract

Fish discards are of major concern in new EU policies. Alternatives for the management of the new biomass that has to be landed is compulsory. The production of bioactive compounds from fish protein hydrolysates (FPH) has been explored in recent years. However, the viability of Scyliorhinus canicula discards, which might account for up to 90–100% of captures in mixed trawler, gillnet, and longline industrial fisheries, to produce FPH from the muscle with bioactivities has still not been studied in terms of the optimization of the experimental conditions to enhance its production. The effect of pH and temperature on the hydrolysis of the S. canicula muscle was mediated by three commercial proteases using response surface methodology. Temperatures of 64.6 °C and 60.8 °C and pHs of 9.40 and 8.90 were established as the best hydrolysis conditions for Alcalase and Esperase, respectively. Optimization of the best conditions for the maximization of antihypertensive and antioxidant activities was performed. Higher Angiotensin-converting enzyme (ACE) activity was found with Esperase. The pH optimum and temperature optimum for antioxidants were 55 °C/pH8.0 for ABTS/DPPH-Esperase, 63.1 °C/pH9.0 for DPPH-Alcalase, and 55 °C/pH9.0 for ABTS-Alcalase. No hydrolysis was detected when using Protamex. View Full-Text
Keywords: Common Fishery Policy; fish discards; Scyliorhinus canicula muscle by-products; fish protein hydrolysates; enzyme hydrolysis; response surface methodology; antihypertensive activity; antioxidant activity Common Fishery Policy; fish discards; Scyliorhinus canicula muscle by-products; fish protein hydrolysates; enzyme hydrolysis; response surface methodology; antihypertensive activity; antioxidant activity
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This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. (CC BY 4.0).

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MDPI and ACS Style

Vázquez, J.A.; Blanco, M.; Massa, A.E.; Amado, I.R.; Pérez-Martín, R.I. Production of Fish Protein Hydrolysates from Scyliorhinus canicula Discards with Antihypertensive and Antioxidant Activities by Enzymatic Hydrolysis and Mathematical Optimization Using Response Surface Methodology. Mar. Drugs 2017, 15, 306.

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