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Mar. Drugs 2017, 15(4), 115; doi:10.3390/md15040115

In Vitro Antioxidant Activities of Enzymatic Hydrolysate from Schizochytrium sp. and Its Hepatoprotective Effects on Acute Alcohol-Induced Liver Injury In Vivo

1
The Key Lab of Analysis and Detection Technology for Food Safety of the MOE, College of Chemistry, Fuzhou University, Fuzhou 350108, China
2
College of Biological Science and Technology, Fuzhou University, Fuzhou 350108, China
*
Author to whom correspondence should be addressed.
Academic Editors: Se-Kwon Kim and Anake Kijjoa
Received: 1 October 2016 / Revised: 6 April 2017 / Accepted: 7 April 2017 / Published: 10 April 2017
(This article belongs to the Special Issue Marine Proteins and Peptides)
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Abstract

Schizochytrium protein hydrolysate (SPH) was prepared through stepwise enzymatic hydrolysis by alcalase and flavourzyme sequentially. The proportion of hydrophobic amino acids of SPH was 34.71%. The molecular weight (MW) of SPH was principally concentrated at 180–3000 Da (52.29%). SPH was divided into two fractions by ultrafiltration: SPH-I (MW < 3 kDa) and SPH-II (MW > 3 kDa). Besides showing lipid peroxidation inhibitory activity in vitro, SPH-I exhibited high DPPH and ABTS radicals scavenging activities with IC50 of 350 μg/mL and 17.5 μg/mL, respectively. In addition, the antioxidant activity of SPH-I was estimated in vivo using the model of acute alcohol-induced liver injury in mice. For the hepatoprotective effects, oral administration of SPH-I at different concentrations (100, 300 mg/kg BW) to the mice subjected to alcohol significantly decreased serum alanine aminotransferase (ALT) and aspartate aminotransferase (AST) activities and hepatic malondialdehyde (MDA) level compared to the untreated mice. Besides, SPH-I could effectively restore the hepatic superoxide dismutase (SOD), catalase (CAT), and glutathione peroxidase (GSH-Px) activities and glutathione (GSH) level. Results suggested that SPH was rich in biopeptides that could be exploited as antioxidant molecules against oxidative stress in human body. View Full-Text
Keywords: Schizochytrium; protein hydrolysate; antioxidant; hepatoprotective effects; alcohol-induced liver injury Schizochytrium; protein hydrolysate; antioxidant; hepatoprotective effects; alcohol-induced liver injury
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Cai, X.; Yan, A.; Fu, N.; Wang, S. In Vitro Antioxidant Activities of Enzymatic Hydrolysate from Schizochytrium sp. and Its Hepatoprotective Effects on Acute Alcohol-Induced Liver Injury In Vivo. Mar. Drugs 2017, 15, 115.

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