Bioactive Pyridone Alkaloids from a Deep-Sea-Derived Fungus Arthrinium sp. UJNMF0008

Abstract

Eight new 4-hydroxy-2-pyridone alkaloids arthpyrones D–K (1–8), along with two known analogues apiosporamide (9) and arthpyrone B (10), were isolated from a deep-sea-derived fungus Arthrinium sp. UJNMF0008. The structures of the isolated compounds were elucidated on the basis of spectroscopic methods with that of 1 being established by chemical transformation and X-ray diffraction analysis. Compounds 1 and 2 bore an ester functionality linking the pyridone and decalin moieties first reported in this class of metabolites, while 3 and 4 incorporated a rare natural hexa- or tetrahydrobenzofuro[3,2-c]pyridin-3(2H)-one motif. Compounds 3–6 and 9 exhibited moderate to significant antibacterial activity against Mycobacterium smegmatis and Staphylococcus aureus with IC₅₀ values ranging from 1.66–42.8 μM, while 9 displayed cytotoxicity against two human osteosarcoma cell lines (U2OS and MG63) with IC₅₀ values of 19.3 and 11.7 μM, respectively.

1. Introduction

The increasing antibiotic resistance and high spreading rate of pathogenic bacteria have become severe public healthcare threats globally, and the efforts towards new antibacterial drugs remain a pressing task [1]. Marine fungi have been considered to be an invaluable resource for bioactive compounds and play an important role in the search for novel antimicrobial compounds [1,2,3]. Fungi of the genus Arthrinium are widely distributed throughout the world and have proven to produce diverse secondary metabolites with a variety of bioactivities, including cytotoxicity [4,5,6,7], antimicrobial [8], anti-HSV [9], AChE inhibitory [4], COX-2 inhibitory [10], syncytium formation inhibitory [11] and Maxi-K channel modulatory [12] activities, as well as lethality against brine shrimp [13].

During the course of our ongoing pursuit for antimicrobial compounds from marine-derived fungi, an Arthrinium sp. UJNMF0008 from a deep-sea sediment sample, collected in the South China Sea (17°55′00″ N, 115°55′31″ E; 3858 m depth), came to our attention owing to its strong inhibitory activity against Staphylococcus aureus. Further chemical investigation on a large-scale culture of this fungal strain led to the isolation of eight previously unreported 4-hydroxy-2-pyridone alkaloids. arthpyrones D–K (1–8), together with two previously reported co-metabolites, apiosporamide (9) [14,15] and arthpyrone B (10) [4]. The structures (Figure 1) of the isolated compounds were characterized on the basis of comprehensive spectroscopic analyses, and their absolute configurations were assigned by different means including ECD comparison, chemical transformation and X-ray crystallography. All the isolates were tested for their antimicrobial, cytotoxic and acetylcholinesterase (AChE) inhibitory activities. The assay results established that 3–6 and 9 were mild to significant antibacterial agents against Mycobacterium smegmatis and S. aureus, while 9 showed moderate cytotoxicity against two human osteosarcoma cell lines U2OS and MG63.

2. Results and discussion

2.1. Structure Elucidation

Compound 1 was obtained as white powder. The HR-ESIMS ion for 1 at m/z 444.2032 ([M − H]⁻, calcd. 444.2028) suggested a molecular formula of C₂₄H₃₁NO₇, which was 16 amu more than that of its co-metabolite arthpyrone B (10) [4], supportive of an oxygenated analogue. Detailed analysis of the ¹H and ¹³C NMR data (

Table 1. ¹H NMR (600 MHz) data for 1–4 (CD₃OD).

Positon	1	2	3	4
	δH, mult. (J in Hz)	δH, mult. (J in Hz)	δH, mult. (J in Hz)	δH, mult. (J in Hz)
1a	2.11, m	2.07, m	1.93, m	1.98, m
1b	1.08, m	1.07, m	0.90, m	0.96, m
2a	1.78, m	1.79, m	1.76, m	1.75, m
2b	1.01, m	1.01, m	1.05, m	1.03, m
3	1.52, m	1.54, m	1.52, m	1.49, m
4a	1.74, m	1.77, m	1.76, m	1.73, m
4b	0.78, q (12.2)	0.78, q (12.4)	0.81, q (12.3)	0.79, q (12.4)
5	1.80, m	1.82, m	1.83, m	1.84, m
6	5.41, brd (9.9)	5.41, brd (9.9)	5.41, brd (9.8)	5.38, brd (9.8)
7	5.61, ddd (9.9, 4.5, 2.8)	5.62, ddd (9.9, 4.4, 2.8)	5.61,m	5.57, ddd (9.8, 4.5, 2.7)
8	2.76, m	2.79, m	2.84, m	2.73, m
9	2.90, dd (11.7,5.9)	2.88, dd (11.6, 5.9)	4.44, dd (11.3, 5.8)	4.07, dd (11.3,5.7)
10	1.39, qd (11.3, 2.5)	1.38, qd (11.5, 2.3)	1.57, qd (11.2, 2.0)	1.54, qd (11.3, 2.6)
11	0.93, d (6.6)	0.93, d (6.6)	0.94, d (6.5)	0.92, d (6.5)
12	1.11, d (7.0)	1.08, d (7.1)	0.83, d (7.2)	0.76, d (7.1)
17	7.38, s	7.31, s	7.69, s	7.96, s
21	3.74, d (2.4)	3.71, d (7.0)	5.15, d (10.1)
22	4.60, brs	3.62, dd (7.0, 6.2)	3.91, dd (10.1, 2.9)	4.68, d (3.8)
23	3.84, ddd (11.6, 5.1, 2.4)	3.69, m	4.09, m	3.95, ddd (10.8, 3.8, 3.2)
24a	1.86, m	2.12, m	2.07, m	2.03, m
24b	1.34, m	1.54, m	1.72, m	1.89, m
25a	1.86, m	2.43, ddd (14.1, 7.9, 3.7)	2.88, m	2.69, m
25b	1.75, m	1.67, ddd (14.1, 9.3, 3.8)	1.51, m	2.51, m

and

Table 2. ¹³C NMR (150 MHz) data for 1–8 (CD₃OD).

Position	1	2	3	4	5	6	7	8
	δc, Type	δc, Type	δc, Type	δc, Type	δc, Type	δc, Type	δc, Type	δc, Type
1	30.7, CH2	30.8, CH2	31.0, CH2	30.8, CH2	31.0, CH2	31.0, CH2	30.4, CH2	30.4, CH2
2	36.5, CH2	36.5, CH2	36.6, CH2	36.7, CH2	36.6, CH2	36.6, CH2	30.8, CH2	34.6, CH2
3	34.3, CH	34.3, CH	34.4, CH	34.4, CH	34.4, CH	34.4, CH	42.0, CH	41.7, CH
4	43.0, CH2	43.0, CH2	43.2, CH2	43.2, CH2	43.2, CH2	43.2, CH2	37.4, CH2	79.6, CH
5	43.2, CH	43.2, CH	43.2, CH	43.4, CH	43.2, CH	43.2, CH	42.7, CH	49.8, CH
6	131.6, CH	131.6, CH	131.7, CH	131.7, CH	131.7, CH	131.7, CH	131.6, CH	127.1, CH
7	132.2, CH	132.2, CH	132.7, CH	132.7, CH	132.7, CH	132.6, CH	132.8, CH	133.3, CH
8	33.8, CH	33.7, CH	32.4, CH	32.9, CH	32.5, CH	32.5, CH	32.4, CH	32.0, CH
9	50.3, CH	50.3, CH	54.2, CH	55.5, CH	54.3, CH	54.3, CH	54.2, CH	54.1, CH
10	37.7, CH	37.7, CH	37.6, CH	38.0, CH	37.6, CH	37.6, CH	37.9, CH	35.9, CH
11	22.9, CH3	22.9, CH3	23.0, CH3	23.0, CH3	23.0, CH3	23.0, CH3	68.6, CH2	19.3, CH3
12	18.3, CH3	18.2, CH3	18.3, CH3	18.3, CH3	18.4, CH3	18.4, CH3	18.4, CH3	18.3, CH3
13	172.9, C	173.2, C	212.1, C	203.8, C	211.9, C	211.9, C	211.8, C	211.6, C
14	123.8, C	126.4, C	108.1, C	110.7, C	108.9, C	108.5, C	108.7, C	108.8, C
15	158.2, C	160.7, C	164.3, C	163.4, C	163.7, C	164.0, C	163.9, C	164.0, C
17	129.8, CH	132.2, CH	141.0, CH	133.3, CH	143.2, CH	144.2, CH	139.9, CH	139.9, CH
18	115.6, C	115.7, C	118.3, C	115.4, C	114.3, C	111.4, C	116.6, C	116.7, C
19	160.8, C	161.9, C	177.2, C	166.4, C	179.8, C	179.5, C	179.3, C	179.4, C
20	70.6, C	78.5, C	76.9, C	116.6, C	76.3, C	82.4, C	70.4, C	70.4, C
21	70.0, CH	77.3, CH	67.7, CH	156.2, C	78.7, CH	75.2, CH	60.5, CH	60.5, CH
22	85.2, CH	75.3, CH	74.1, CH	65.3, CH	86.6, CH	75.2, CH	57.6, CH	57.6, CH
23	71.6, CH	72.6, CH	71.3, CH	70.8, CH	72.4, CH	72.4, CH	67.1, CH	67.1, CH
24	27.7, CH2	27.6, CH2	27.1, CH2	26.7, CH2	28.2, CH2	26.1, CH2	25.8, CH2	25.8, CH2
25	37.6, CH2	31.1, CH2	31.2, CH2	18.4, CH2	30.4, CH2	23.7, CH2	31.6, CH2	31.6, CH2
OCH3					60.5, CH3	51.0, CH3

) indicated that 1 incorporated a skeleton similar to that of arthpyrone B (10) [4], with a likely additional ester group as supported by the NMR differences of the remarkably upfield shifted C-13 resonance (from 208.4 in 10 to 172.9 in 1) and of adjacent C-9, C-14, C-15, C-17 and C-19 signals. This deduction was further confirmed by inspection of 2D NMR data. The COSY correlations (Figure 2) of 1 revealed two spin coupling systems from H₂-1–H-10 (including branch fragments from H-3–H₃-11 and H-8–H₃-12) of a decalin moiety and from H-21–H₂-25. Subsequent examination of HMBC data (Figure 2) revealed key correlations from H-17 to C-14, C-15, C-18, C-19 and C-20, H-21 to C-18, C-20 and C-25, H-22 to C-19 and H-9 to C-13, leading to the construction of Fragments A and B that were identical to those in 10. The two fragments were linked via an ester bond as demonstrated by the MS difference (amu 16) and ¹³C NMR chemical shift variations for C-13 (Δδ_C −35.5) and C-14 (Δδ_C 10.9) between 1 and 10. The planar structure of 1 was thus established with an ester bridge between the pyridone and decalin moieties.

The relative stereochemistry of 1 was assigned to be identical to that of arthpyrone B (10) based on analyses of NOESY data and ¹H–¹H couplings. More specifically, the key NOESY correlations from H-10 (δ_H 1.39) to both H-4b (δ_H 0.78) and H₃-12 (δ_H 1.11) (1,3-diaxial relationship) suggested that H-4b, H-10 and CH₃-12 were axially located on the same side. Meanwhile, the correlations from H-5 (δ_H 1.80) to both H-3 (δ_H 1.52) and H-9 (δ_H 2.90) also indicated their axial and co-facial positions. The relative configuration of Fragment B was thus defined with a trans-conjunction. In addition, the relative configuration of Fragment A was supported by the NOESY correlations from H-25a (δ_H 1.86) to both H-21 (δ_H 3.74) and H-23 (δ_H 3.84), as well as J_21,22 (2.4 Hz) and J_22,23 (2.4 Hz). Unfortunately, the stereochemical relationship between Fragments A and B could not be determined due to the interruption of the achiral pyridone unit. However, a further transformation of 10 to 1 (see the Experimental Section) via Baeyer–Villiger oxidation connected the chemical relationship between the two co-metabolites and also confirmed the structural assignment. Finally, a suitable single crystal of 1 was obtained from the MeOH-H₂O binary system and subject to X-ray diffraction analysis, which not only verified the structure of 1, but also established its absolute stereochemistry (Figure 3) as 3R, 5S, 8R, 9R, 10R, 20R, 21S, 22S, 23S (Flack parameter, 0.0(3)). Compound 1 was thus unambiguously characterized and was named arthpyrone D, following arthpyrones A−C, isolated from another fungus of the same genus [4]. It is interesting to point out that the assigned C-20 configuration for 1 is opposite that described for arthpyrones A and B [4]. By careful examination of the reported ORTEP view of arthpyrone A, it is clear that both arthpyrones A and B also bear a 20R absolute configuration, while the authors inverted the C-20 stereochemistry by mistake when drawing the structures in two dimensions.

Compound 2 was isolated as a pale yellow powder, and its molecular formula C₂₄H₃₃NO₈ was established from the HR-ESIMS ion at m/z 462.2143 ([M − H]⁻, calcd. 462.2133), being 18 mass units more than that of 1. The NMR data of 2 resembled those of 1 (Table 1 and Table 2), and the obvious differences were the downfield shifted signals of C-20 (δ_C 78.5 in 2 and 70.6 in 1) and C-21 (δ_C 77.3 in 2 and δ_C 70.0 in 1), along with the upfield shifted resonance of C-22 (δ_C 75.3 in 2 and δ_C 85.2 in 1). Further examination of COSY data (Figure 4) revealed two structural motifs of the decalin and CH-21–CH₂-25 part, which were the same as those in 1. The pivotal HMBC correlations (Figure 4) from H-9 (δ_H 2.88) to C-13, H-17 (δ_H 7.31) to C-14, C-15, C-18, C-19 and C-20 and H-21 (δ_H 3.71) to C-18, C-20 and C-25 were also consistent with those observed for 1. Therefore, the aforementioned MS and NMR differences, the absence of HMBC correlation from H-22 to C-19 and the more polar nature of 2 all supported the planar structure of 2 without the epoxy bridge between C-19 and C-22. The relative configuration of 2 was assigned on the basis of NOESY data (Figure 4). The configuration of the decalin moiety was identical to that of 1 as indicated by NOESY correlations and similar NMR data. The NOESY correlations from H-25b (δ_H 1.67) to both H-21 (δ_H 3.71) and H-23 (δ_H 3.69) suggested their axial and co-planar nature, while those from H-22 (δ_H 3.62) to both H-17 (δ_H 7.31) and H-24b (δ_H 1.54) supported that H-22, H-24b and the pyridone unit were axially located on the other side of the hexane ring. The structure of 2 was hence elucidated as shown in Figure 4.

Compound 3 was assigned a molecular formula of C₂₄H₃₁NO₆ by HR-ESIMS m/z 428.2074 ([M − H]⁻, calcd. 428.2079), suggestive of an isomer of apiosporamide (9) [14,15]. Analysis of the NMR data (Table 1 and Table 2) for 3 revealed the presence of a decalin moiety as indicated by a close resemblance between its NMR data and those of 9, and the main NMR differences between the two co-metabolites observed for the left-side Fragment A. Further inspection of COSY data (Figure 5) confirmed the existence of the decalin motif and also established the same spin coupling system from H-21–H₂-25 as that in 9. In addition, the HMBC correlations (Figure 5) from H-9 (δ_H 4.44) to C-13 and C-14 verified the connection between Fragments A and B, while those from H-17 (δ_H 7.69) to C-14, C-15, C-18, C-19 and C-20, along with those from H-25b (δ_H 1.51) to C-20 and C-21, enabled the assembly of the pyridone ring and the cyclohexane moiety, as well as their linkage from C-18–C-20. Moreover, four exchangeable protons were observed in the ¹H NMR spectrum (Supplementary Information Table S1), measured in DMSO-d₆, which were assigned for NH, 20-OH, 22-OH and 23-OH, respectively. This was supported by HMBC and COSY correlations (Figure 5) from NH to H-17, 20-OH to C-20, H-22 to 22-OH and H-23 to 23-OH. The above-mentioned analyses accounted for all but one oxygen atom, which was ascribed to the formation of an epoxy bridge between C-19 and C-21, as was also supported by the downfield shifted chemical shift of C-19 (δ_C 177.2) and upfield shifted chemical shift of C-21 (δ_C 67.7) compared with those in 2 (Table 2). The relative configuration of 3 was established via analyses of NOESY data (Figure 5) and ¹H–¹H couplings (Table 1). The similarity between the ¹H NMR spectra of the decalin moiety of 3 and 9 supported the common relative stereochemistry, as was also confirmed by NOESY correlations. The crucial NOESY correlations from H-21–H-25a and H-22–H-24a indicated the 1,3-diaxial relationship of the two proton pairs, which in combination with the proton coupling constants of J₂₁,₂₂ (10.1 Hz) and J₂₂,₂₃ (2.9 Hz) defined the relative configuration of the polyoxygenated cyclohexane moiety. The structure of 3 was thereby characterized to feature a rare hexahydrobenzofuro[3,2-c]pyridin-3(2H)-one fragment with very few examples reported from nature [16,17,18].

The molecular formula of 4 was determined as C₂₄H₂₉NO₅ by HR-ESIMS m/z 410.1974 ([M − H]⁻, calcd. 410.1973), indicative of a dehydrated analogue of 3. Analysis of the NMR data (Table 1 and Table 2) for 4 confirmed this hypothesis, with diagnostic signals for two quaternary sp² carbons (δ_C 116.6 and 156.2) in 4 instead of the oxyquaternary sp³ carbons at δ_C 76.9 (C-20) and δ_C 67.7 (C-21) in 3. This observation led to the suggestion that the furan ring is formed between the cyclohexadiol and 2-piperidone rings and extended the conjugation system of the pyridone chromophore, which was further confirmed by the UV data of 4 with an absorption peak red-shifted to 367 nm. Further examination of COSY and HMBC correlations (Supplementary Information Figures S42 and S43) corroborated the aforementioned structural assignment. The similarity of the NMR data of the decalin moieties of 4 and 3 suggested the common relative configurations as also confirmed by NOESY correlations (Supplementary Information Figure S44). The crucial NOESY correlation from H-23–H-25b and proton coupling constants of J₂₂,₂₃ (3.8 Hz) and J₂₃,_24a (10.8 Hz) defined the relative configurations at C-22 and C-23 as shown in Figure 1.

Compounds 5 and 6 were assigned the same molecular formula of C₂₅H₃₅NO₇ by their HR-ESIMS m/z 460.2349 and 460.2337 ([M − H]⁻, calcd. 460.2341), respectively, suggestive of a pair of isomers. The NMR data (Table 2 and

Table 3. ¹H NMR (600 MHz) data for 5–8 (CD₃OD).

Positon	5	6	7	8
	δH, mult. (J in Hz)	δH, mult. (J in Hz)	δH, mult. (J in Hz)	δH, mult. (J in Hz)
1a	1.91, m	1.91, m	1.98, m	1.86, m
1b	0.87, m	0.88, m	0.90, m	0.90, m
2a	1.74, m	1.74, m	1.81, m	1.74, m
2b	1.03, m	1.03, m	1.05, m	1.14, dq (13.1, 3.5)
3	1.51, m	1.50, m	1.60, m	1.39, m
4a	1.74, m	1.74, m	1.84, m	2.73, dd (9.9, 9.9)
4b	0.79, q (12.3)	0.79, q (12.4)	0.82, m
5	1.81, m	1.81, m	1.82, m	1.77, m
6	5.39, brd (9.8)	5.40,brd (9.8)	5.42, brd (9.8)	6.00, brd (10.1)
7	5.59, ddd (9.8, 4.3, 2.7)	5.59, ddd (9.8, 4.4, 2.7)	5.60, ddd (9.8, 4.4, 2.5)	5.68, ddd (10.1, 4.4, 2.6)
8	2.82, m	2.81, m	2.83, m	2.81, m
9	4.41, dd (11.3, 5.8)	4.41, dd (11.1, 5.6)	4.43, dd (11.3, 5.7)	4.46, dd (11.2, 5.8)
10	1.55, m	1.55, m	1.58, m	1.71, m
11	0.92, d (6.5)	0.92, d (6.5)	3.39, dd (6.0, 1.2)	1.03, d (6.4)
12	0.81, d (7.2)	0.81, d (7.2)	0.82, d (7.2)	0.82, d (7.2)
17	8.00, s	7.77, s	7.57, s	7.56, s
21	3.87, d (7.9)	4.20, s	3.63, d (3.6)	3.63, d (3.8)
22	3.35, dd (9.8, 7.9)	3.65. m	3.41, t (3.6)	3.41, dd, (3.8, 2.8)
23	3.69, m	3.73, m	4.12, m	4.12, m
24a	1.91, m	1.98, m	1.81, m	1.81, m
24b	1.31, m	1.55, m	1.34, m	1.33, m
25a	2.82, m	2.59, m	2.20, m	2.20, m
25b	1.48, m	1.76, m	1.69, ddd (14.2, 10.2, 2.8)	1.69, m
OCH3	3.63, s	3.18, s

) for 5 revealed high similarity to those of apiosporamide (9) [14,15], and the major differences were attributable to the shielded signals for the oxirane ring in 9 instead of two oxymethines (δ_H 3.87 and 3.35; δ_C 78.7 and 86.6) and a methoxy group (δ_H 3.63; δ_C 60.5) in 5. Subsequent analysis of COSY and HMBC correlations (Supplementary Information Figures S50 and S51) corroborated the aforementioned observations with a key HMBC correlation from the methoxy protons to C-22 (δ_C 86.6) and established the planar structure of 5. The relative stereochemistry of 5 was determined to be identical to that of 2 based on the comparison of their NMR spectra, including crucial ¹H–¹H couplings between the corresponding chiral centers, which were further confirmed by examination of the NOESY spectrum (Supplementary Information Figure S52). Analysis of the NMR data (Table 2 and Table 3) for 6 suggested that it was a methoxy regio-isomer of 5, and the methoxy group was determined to locate at C-20 (δ_C 82.4) via the HMBC correlation from the methoxy protons (δ_H 3.18) to C-20. Detailed inspection of COSY and HMBC spectra (Supplementary Information Figures S57 and S58) verified the planar structure of 6, and the relative configuration was also determined to be the same as that of 5 based on analyses of key ¹H–¹H couplings and NOESY correlations (Supplementary Information Figure S59).

Compounds 7 and 8 were determined to possess the same molecular formula C₂₄H₃₁NO₇ by their HR-ESIMS m/z 444.2025 and 444.2030 ([M − H]⁻, calcd. 444.2028), respectively, indicative of oxygenated analogues of apiosporamide (9) [14,15]. Analysis of the NMR data (Table 2 and Table 3) for 7 confirmed this hypothesis with diagnostic signals for an oxymethylene (δ_H 3.39, 2H; δ_C 68.6) in 7 instead of the CH₃-11 group in 9. Good resemblance between the remaining NMR data for 7 and 9 suggested the assignment of common structural features between the two co-metabolites and indicated their same relative stereochemistry. The structure with the relative configuration of 7 was finally confirmed by further examination of 2D NMR data, especially COSY, HMBC and NOESY spectra (Supplementary Information Figures S64–S66). Similarly, the oxidation of 8 occurred at C-4 as supported by the COSY correlations from H-4 (δ_H 2.73) to H-3 (δ_H 1.39) and H-5 (δ_H 1.77), as well as the HMBC correlation from H₃-11 (δ_H 1.03) to C-4 (δ_C 79.6). The coupling constants of H-4 (dd, J = 9.9 Hz) indicated that it was axially located, and thus, the hydroxyl group was equatorially oriented. The low chemical shift of H-4 (<3.0 ppm) was due to its axial position, which located it in the shielding zone of C2-C3 and C5-C6 bonds. The similarity between the remaining NMR data for 8 and 7, especially key ¹H–¹H coupling constants, supported common structural features, including configurations of the stereocenters, between the two co-metabolites. The structure of 8 with the relative configuration was further confirmed by analysis of 2D NMR data (Supplementary Information Figures S71–S73).

Compounds 9 and 10 were identified to be the previously reported apiosporamide [14,15] and arthpyrone B [4], respectively, based on comprehensive spectroscopic analyses including MS, NMR, [α]_D and ECD. While the absolute configuration of 1 was established by X-ray analysis and chemical transformation, those of the other analogues were assigned mainly by analysis of their ECD data. The absolute configurations of 3 and 5–8, which have the same 3-acyl pyridone chromophore, were characterized by their ECD spectra, which are similar to that of 9 (Figure 6), whose absolute structure had been confirmed by total synthesis of its enantiomer [14,15]. The absolute configurations of the stereocenters of 2 and 4 were proposed on biogenetic grounds (Scheme 1).

With all these metabolites in hand, we were able to propose a plausible biosynthetic pathway for the new analogues. Compound 1 could be obtained from 10 via one-step Baeyer–Villiger oxidation, while selective oxidation of 9 at C-11 and C-4 would afford 7 and 8, respectively. The likely biosynthetic origin of 2–6 is outlined in Scheme 1. Protonation of the epoxy group of 9 would give the intermediate (i) that could undergo intramolecular nucleophilic substitution by 19-OH, followed by deprotonation to yield 3. Subsequent dehydration of 3 would furnish 4. The intermediate (i) could also experience ring opening by hydrolysis followed by deprotonation to afford the intermediate (ii), which was not obtained in the current study. The intermediate (ii) could produce 2 via Baeyer–Villiger oxidation or undergo selective methylation to yield 5 or 6.

2.2. Biological Activity

Compounds 1–10 were tested against two Gram-positive strains Mycobacterium smegmatis ATCC 607 and Staphylococcus aureus ATCC 25923, two Gram-negative strains Escherichia coli ATCC 8739 and Pseudomonas aeruginosa ATCC 9027 and a yeast Candida albicans ATCC10231. Compounds 3–6 and 9 exhibited antibacterial activity against M. smegmatis and S. aureus with IC₅₀ values ranging from 1.66–42.8 μM (

Table 4. Antibacterial activity of 3–6 and 9 (IC₅₀, μM) ^a.

Compounds	MS	SA
3	11.4	8.97
4	>50	42.8
5	19.4	8.37
6	35.3	14.1
9	2.20	1.66

^a Ceftriaxone sodium was used as a positive control (IC₅₀ < 1.0 μM); MS: Mycobacterium smegmatis ATCC607; SA: Staphylococcus aureus ATCC25923.

), while none of the tested compounds showed significant antimicrobial activity against E. coli, P. aeruginosa and C. albicans at 50 μM. Expanding our biological activity evaluations, these isolates were also screened for AChE inhibitory and cytotoxic activities against two human osteosarcoma cell lines U2OS and MG63. Only 9 displayed mild cytotoxicity against the two cell lines with IC₅₀ values of 19.3 and 11.7 μM.

3. Experimental Section

3.1. General Experimental Procedures

NMR spectra were recorded on a Bruker Avance DRX600 NMR spectrometer with residual solvent peaks as references (CD₃OD: δ_H 3.31, δ_C 49.00; DMSO-d₆: δ_H 2.50, δ_C 39.52). Optical rotations were measured on a Rudolph VI polarimeter. UV spectra were obtained using a Shimadzu UV-2600 spectrophotometer. The X-ray diffraction analysis was performed on an Oxford Gemini E Eos CCD detector with graphite monochromated Cu Kα radiation (λ = 1.54178 Å). ECD spectra were acquired on a Chirascan circular dichroism spectrometer (Applied Photophysics). ESIMS analyses were conducted on an Agilent 1260–6460 Triple Quad LC-MS spectrometer. HR-ESIMS spectra were obtained on an Agilent 6545 Q-TOF mass spectrometer. Semipreparative HPLC separations were carried out on an Agilent 1260 series using a YMC-Pack ODS-A column (250 × 10 mm, 5 μm). Column chromatography (CC) was performed on silica gel (200–300 mesh, Yantai Jiangyou Silica Gel Development Co., Yantai, China), reversed phase C₁₈ silica gel (Merck KGaA, Darmstadt, Germany) and Sephadex LH-20 gel (GE Healthcare Bio-Sciences AB, Uppsala, Sweden).

3.2. Fungal Material

The fungus strain UJNMF0008 was isolated from a marine sediment sample collected in the South China Sea (17°55′00″ N, 115°55′31″ E; 3858 m depth). This strain was identified as an Arthrinium sp. based on morphological traits and a molecular biological protocol by DNA amplification and comparison of its ITS region sequence with the GenBank database (100% similarity with Arthrinium sp. zzz1842 (HQ696050.1)). The BLAST sequenced data were deposited at GenBank (No. MG010382). The strain was deposited at China General Microbiological Culture Collection Center (CGMCCC), Institute of Microbiology, Chinese Academy of Sciences.

3.3. Fermentation and Extraction

Arthrinium sp. UJNMF0008 from a PDA culture plate was inoculated in 500-mL Erlenmeyer flasks containing 150 mL soluble starch medium (1% glucose, 0.1% soluble starch, 1% MgSO₄, 0.1% KH₂PO₄, 0.1% peptone and 3% sea salt) at 28 °C on a rotary shaker at 180 rpm for 3 days as seed cultures. Then, each of the seed cultures (20 mL) was transferred into autoclaved 1-L Erlenmeyer flasks with solid rice medium (each flasks contained 80 g commercially available rice, 0.4 g yeast extract, 0.4 g glucose, and 120 mL water with 3% sea salt). After that, the strain was incubated statically for 30 days at 28 °C.

After fermentation, the total 4.8 kg rice culture was crushed and extracted with 15.0 L 95% EtOH three times. The EtOH extract was evaporated under reduced pressure to afford an aqueous solution and then extracted with 2.0 L ethyl acetate three times to give 80 g crude gum.

3.4. Isolation and Purification

The extract was fractionated by a silica gel column eluting with step gradient CH₂Cl₂-MeOH (v/v 100:0, 98:2, 95:5, 90:10, 80:20, 70:30, 50:50 and 0:100, each 8.0 L) to give ten fractions (Fr.1–Fr.10) based on TLC and HPLC analysis. Fr.7 (12.2 g) was repeatedly applied to the silica gel column with step gradient CH₂Cl₂-(CH₃)₂CO (v/v 100:0–0:100) and divided into three subfractions (Fr.7-1–Fr.7-3). Fr.7-2 (5.8 g) was fractionated by the MCI gel column eluted with gradient MeOH-H₂O to give four subfractions (Fr.7-2-1–Fr.7-2-4). Fr.7-2-4-1 (68.5 mg) obtained from Fr.7-2-4 (0.9 g) via Sephadex LH-20 CC (in MeOH) was further purified by HPLC eluting with MeOH-H₂O (v/v 73:27, 2.5 mL min⁻¹) to give 9 (t_R = 34.6 min, 45.2 mg), while Fr.7-2-4-2 (40.2 mg) was further separated by HPLC eluting with MeOH-H₂O (v/v 71:29, 2.5 mL min⁻¹) to afford 4 (t_R = 22.2 min, 2.8 mg), 3 (t_R = 32.3 min, 1.6 mg) and 5 (t_R = 48.3 min, 2.5 mg). Fr.8 (15.9 g) was subject to silica gel CC with step gradient CH₂Cl₂-(CH₃)₂CO (v/v 100:0–0:100) and divided into eight subfractions (Fr.8-1–Fr.8-8). Fr.8-2 (3.2 g) was applied to the MCI column eluted with gradient MeOH-H₂O to give four subfractions (Fr.8-2-1–Fr.8-2-4). Fr.8-2-3 (0.9 g) was further divided by Sephadex LH-20 CC eluting with MeOH-CH₂Cl₂ (v/v 1:1) and then purified by HPLC eluting with MeOH-H₂O-CH₃CO₂H (v/v/v 50:50:10⁻⁴, 2.5 mL min⁻¹) to afford 7 (t_R = 21.1 min, 48.2 mg) and 8 (t_R = 23.9 min, 54.6 mg). Fr.8-2-4 (432.4 mg) was also fractionated by Sephadex LH-20 CC eluting with MeOH-CH₂Cl₂ (v/v 1:1) and then purified by HPLC eluting with MeOH-H₂O-CH₃CO₂H (v/v/v 75:25:10⁻⁴, 2.5 mL min⁻¹) to yield 6 (t_R = 33.1 min, 1.6 mg). Fr.8-3 (6.4 g) was applied to MPLC with an ODS column eluting with gradient MeOH-H₂O (v/v 50:50–70:30) to obtain 1 (3.3 g). Fr.8-8 (190.0 mg) was separated by Sephadex LH-20 CC eluting with MeOH-CH₂Cl₂ (v/v 1:1) to obtain two subfractions (Fr.8-8-1–Fr.8-8-2). Then, Fr.8-8-1 (122.6 mg) was purified by HPLC eluting with MeOH-H₂O-CH₃CO₂H (v/v/v 64:36:10⁻⁴, 2.5 mL min⁻¹) to yield 10 (t_R = 33.2 min, 52.6 mg), and Fr.8-8-2 (35.6 mg) was isolated by HPLC eluting with MeOH-H₂O-CH₃CO₂H (v/v/v 68:32:10⁻⁴, 2.5 mL min⁻¹) to yield 2 (t_R = 13.5 min, 4.6 mg).

3.4.1. Arthpyrone D (1)

Colorless crystals; mp 204–207 °C; [α]²⁶_D −23.8 (c 1.0, MeOH); UV (MeOH) λ_max (log ε) 213 (3.96), 289 (3.04) nm; ECD (0.05 mg mL⁻¹, MeOH) λ (Δε) 214 (46.2), 235 (3.4), 283 (1.6) nm; ¹H and ¹³C NMR data, Table 1 and Table 3; (+)-ESIMS m/z 446.1 [M + H]⁺; (−)-HR-ESIMS m/z 444.2032 [M − H]⁻ (calcd. for C₂₄H₃₀NO₇, 444.2028).

3.4.2. Arthpyrone E (2)

Pale yellow powder; [α]²⁶_D −28.9 (c 0.5, MeOH); UV (MeOH) λ_max (log ε) 211 (3.96), 279 (3.02) nm; ECD (0.05 mg mL⁻¹, MeOH) λ (Δε) 212 (27.0), 285 (−1.1) nm; ¹H and ¹³C NMR data, Table 1 and Table 3; (−)-ESIMS m/z 462.0 [M − H]⁻; (−)-HR-ESIMS m/z 462.2143 [M − H]⁻ (calcd. for C₂₄H₃₂NO₈, 462.2133).

3.4.3. Arthpyrone F (3)

Pale yellow powder; [α]²⁶_D −108.7 (c 0.2, MeOH); UV (MeOH) λ_max (log ε) 230 (2.87), 279 (2.35), 332 (2.56) nm; ECD (0.20 mg mL⁻¹, MeOH) λ (Δε) 258 (0.6), 316 (0.8), 341 (−0.7) nm; ¹H and ¹³C NMR data, Table 1 and Table 3; (−)-ESIMS m/z 428.0 [M − H]⁻; (−)-HR-ESIMS m/z 428.2074 [M − H]⁻ (calcd. for C₂₄H₃₀NO₆, 428.2079).

3.4.4. Arthpyrone G (4)

Pale yellow powder; [α]²⁶_D −76.2 (c 0.2, MeOH); UV (MeOH) λ_max (log ε) 228 (3.65), 262 (3.01), 367 (2.67) nm; ECD (0.10 mg mL⁻¹, MeOH) λ (Δε) 238 (2.5); 319 (0.7) nm; ¹H and ¹³C NMR data, Table 1 and Table 3; (−)-ESIMS m/z 410.0 [M − H]⁻; (−)-HR-ESIMS m/z 410.1974 [M − H]⁻ (calcd. for C₂₄H₂₈NO₅, 410.1973).

3.4.5. Arthpyrone H (5)

Pale yellow powder; [α]²⁶_D −90.2 (c 0.2, MeOH); UV (MeOH) λ_max (log ε) 230 (3.07), 277 (2.56), 328 (2.73) nm; ECD (0.20 mg mL⁻¹, MeOH) λ (Δε) 262 (0.4), 311 (2.7) nm;¹H and ¹³C NMR data, Table 2 and Table 3; (−)-ESIMS m/z 460.1 [M − H]⁻; (−)-HR-ESIMS m/z 460.2349 [M − H]⁻ (calcd. for C₂₅H₃₄NO₇, 460.2341).

3.4.6. Arthpyrone I (6)

Pale yellow powder; [α]²⁶_D −76.2 (c 0.1, MeOH); UV (MeOH) λ_max (log ε) 230 (3.14), 278 (2.51), 329 (2.78) nm; ECD (0.10 mg mL⁻¹, MeOH) λ (Δε) 262 (1.8), 312 (3.0) nm; ¹H and ¹³C NMR data, Table 2 and Table 3; (−)-ESIMS m/z 460.1 [M − H]⁻; (−)-HR-ESIMS m/z 460.2337 [M − H]⁻ (calcd. for C₂₅H₃₄NO₇, 460.2341).

3.4.7. Arthpyrone J (7)

Pale yellow powder; [α]²⁶_D −61.2 (c 1.0, MeOH); UV (MeOH) λ_max (log ε) 230 (3.72), 276 (3.17), 330 (3.43) nm; ECD (0.05 mg mL⁻¹, MeOH) λ (Δε) 210 (1.2), 227 (−23.1), 265 (7.5), 310 (9.7), 341 (−3.1) nm; ¹H and ¹³C NMR data, Table 2 and Table 3; (−)-ESIMS m/z 444.0 [M − H]⁻; (−)-HR-ESIMS m/z 444.2025 [M − H]⁻ (calcd. for C₂₄H₃₀NO₇, 444.2028).

3.4.8. Arthpyrone K (8)

Pale yellow powder; [α]²⁶_D −68.7 (c 0.5, MeOH); UV (MeOH) λ_max (log ε) 229 (3.50), 277 (2.98), 330 (3.18) nm; ECD (0.05 mg mL⁻¹, MeOH) λ (Δε) 210 (1.2), 227 (−13.7), 263 (4.3), 306 (6.3), 342(−2.3) nm; ¹H and ¹³C NMR data, Table 2 and Table 3; (−)-ESIMS m/z 444.0 [M − H]⁻; (−)-HR-ESIMS m/z 444.2030 [M − H]⁻ (calcd. for C₂₄H₃₀NO₇, 444.2028).

3.5. Chemical Transformation of 10 to 1

M-chloroperoxybenzoic acid (MCPBA) (31.1 mg, 0.18 mmol) was added to a solution of 10 (25.3 mg, 0.06 mmol) in methanol (2 mL), and the mixture was stirred at 25 °C overnight. Then, the reaction solution was concentrated under reduced pressure, and the residue was purified by HPLC eluting with MeOH-H₂O (v/v 60:40, 2.5 mL min⁻¹) to yield 10r (t_R = 49.7 min, 0.8 mg), which was consistent with 1 in all aspects.

3.6. Antimicrobial Assays

The antimicrobial activity was assayed against the Gram-positive bacterial strains Mycobacterium smegmatis ATCC 607 and Staphylococcus aureus ATCC 25923, Gram-negative Escherichia coli ATCC 8739 and Pseudomonas aeruginosa ATCC 9027 and yeast Candida albicans ATCC10231 by liquid growth inhibition in 96-well microplates. Briefly, precultures of the tested microorganisms were made by inoculating 10 mL of medium (LB medium for bacteria and YM medium for fungus) and incubated for 24 h at 37 °C for bacteria or 48 h at 28 °C for fungus. Then, the cell density was adjusted to 10⁴−10⁵ cfu mL⁻¹ by the corresponding broth. An aliquot of 200 μL of the microbial suspension was distributed in each well containing 2-fold serial dilution of the tested compounds. The plate was incubated at 37 °C for 12 h for the bacteria or at 28 °C for 48 h for the fungus, and the optical density of each well was measured at 600 nm spectrophotometrically. IC₅₀ values were defined as the concentration of a compound resulting in a 50% decrease in the number of microbial cultures compared to the blank control. The experiment was run in three replicates.

3.7. AChE Inhibitory Assay

The AChE inhibitory activities of compounds were tested by using the modified Ellman’s method as described previously [19].

3.8. Cytotoxic Assay

The cytotoxicity of compounds was evaluated using human osteosarcoma U2OS and MG63 cell lines by the MTT method as described previously [20]. Adriamycin was used as a positive control (IC₅₀ 21.1 and 3.56 nM for U2OS and MG63, respectively).

3.9. X-ray Diffraction Analysis of Arthpyrone D (1)

Compound 1 was crystallized in MeOH-H₂O at room temperature. The X-ray crystallographic data were obtained from an Oxford Gemini E Eos CCD detector equipped with a graphite monochromated Cu Kα radiation (λ = 1.54178 Å) at 293(2) K. The structure was solved with the XT structure solution program using intrinsic phasing and refined with the ShelXL refinement package using least squares minimization [21,22]. All non-hydrogen atoms were refined anisotropically. The hydrogen atom positions were geometrically idealized and allowed to ride on their parent atoms. Crystallographic data for 1 were deposited at the Cambridge Crystallographic Data Centre (Deposition No.: CCDC 1813453).

4. Conclusions

Until now, about twenty 4-hydroxy-2-pyridone alkaloids with a decalin ring have been reported and showed significant cytotoxicity, antimicrobial activity and AChE inhibitory activity, but only arthpyrones A–C were isolated from marine-derived fungus [6,14,15,23,24,25,26,27,28,29,30]. Our current study contributed eight new members (1–8) to this structural class from a deep-sea-derived fungus Arthrinium sp. Among this series of fungal metabolites, the ester bridge that connected the pyridone and decalin fragments in 1 and 2 was reported for the first time, while 3 and 4 incorporating the rare hexa- or tetrahydrobenzofuro[3,2-c]pyridin-3(2H)-one ring system were also the first examples of this class of compounds. Our biological activity evaluations established selective compounds as antibacterial agents against Gram-positive M. smegmatis and S. aureus and/or cytotoxic agents toward human osteosarcoma cell lines U2OS and MG63. Knowledge of these rare pyridone alkaloids will be of great interest to the scientific community.